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All Journal Aquacoastmarine BERITA BIOLOGI
Huria Marnis
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Other

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Journal : Aquacoastmarine

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DETEKSI DAN ANALISIS EKSPRESI TRANSGEN (PhGH) PADA IKAN LELE DUMBO (Clarias gariepinus) TRANSGENIK F3 (Detection and Analysis Expression of transgene (PhGH) in Dumbo Catfish (Clarias gariepinus) Transgenic F3) Fery Jaksen Sihotang; Budi Utomo; Indra Lesmana; Huria Marnis
AQUACOASTMARINE Vol 3, No 4 (2015): AQUACOASTMARINE
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Pertanian Universitas Sumatera Utara

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Abstract

Fisheries output need tobe increased to meet the rising demands for fish incomingyears as the increasing of human population. Biotechnology approach has used forincreasing production on aquaculture sector. One of the advances biotechnologies thatused for aquaculture development is transgens technology. Transgenic fish is a speciesthat experience alteration of genetic structure that introduced from other organisms,with the result that change the their genetic function in accordance with the introducedgene. One of the things that support the development of transgenic fish strain formationis the ability of individual transgenic to bequeath the transgenes to the next generation,so that the genetic trait target genes stable in the resulting offspring. This study aims todetect and determine the expression of growth hormone gene (PhGH) on dumbo catfish(Clarias gariepinus) transgenic F3. Transgene detection performed on embryos andlarvae of catfish transgenic F3 using PCR with primers ACT 107 and PhGH2.Transgene expression was analyzed by using reverse transcriptase polymerase chainreaction (RT-PCR) methods with primers β-actin and ACT 107 and PhGH2. Theresults showed that the transgene was detected in some of the embryos and larvaeaccording to Mendel principle. β-actin primer amplification compared to primary ACT107 and PhGH2 showed that transgene can be expressed in some of the embryos andlarvae.Keywords: biotechnology, aquaculture, transgenic, detection, expression, growthhormone
PENGUJIAN PERILAKU PREDATOR DAN ANTI-PREDATOR PADA IKAN LELE (Clarias gariepinus Burchell, 1822) TRANSGENIK F3 Testing Predator and Anti-Predator Behavior in African Catfish (Clarias gariepinus Burchell, 1822) Transgenic F3 Putri Widyawati; Darma Bakti; Desrita Desrita; Huria Marnis
AQUACOASTMARINE Vol 3, No 4 (2015): AQUACOASTMARINE
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Pertanian Universitas Sumatera Utara

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Abstract

African catfish (Clarias gariepinus Burchell, 1822) transgenic containing Pangasius hypothalmus Growth Hormone (PhGH) has better performance compared with non-transgenic. However, before transgenic African catfish are released and utilized, their genetic impact on the natural environment must be examined. Predator avoidance is one of the major fitness traits determining potential environment risk. Transgene detection was performed using the PCR (Polymerase Chain Reaction) method and electrophoresis. Furthermore, the observation of the performance of growth including the growth of the length (the average length of the fish at the end - the average length of the fish at the beginning), the growth in weight (average weight of fish in the end – average fish at the beginning) and the survival rate (the number of fish that live in end / number of fish lived at the beginning). Results indicate that non-trangenic had better predator avoidance than transgenic African catfish. Non-transgenic has a better growth performance than transgenic African catfish and has a higher survival rate. Transgenic African catfish could be used for commercial aquaculture without affecting the natural environment because the increased susceptibility of transgenic to predators would most likely decrease or eliminate the transgenic genotype.Keywords: Clarias gariepinus, Transgenic, Environmental Risk
TRANSMISI TRANSGEN (PhGH) DAN PERFORMA PERTUMBUHAN IKAN LELE (Clarias gariepinus) TRANSGENIK F-3 Transmission of Transgene (PhGH) and Growth Performance Catfish (Clarias gariepinus) Transgenic F-3 Sultan Akbar Habibullah; Zulkifli Nasution; Yunasfi Djayus; Huria Marnis
AQUACOASTMARINE Vol 3, No 4 (2015): AQUACOASTMARINE
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Pertanian Universitas Sumatera Utara

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Abstract

Fish increasing in aquaculture production requires the fulfillment of the need toaccelerate the productivity. Transgenesis technology with the introduction of transgenes(PhGH) can be performed to produce the fish that grow faster and has better performance.Transgene detection was performed using the PCR (Polymerase Chain Reaction) method andelectrophoresis using seed of transgenic catfish F-3. Furthermore, the observation of theperformance of catfish transgenic F-3 by measuring the rate of fertilization (the number offertilized eggs / total eggs), hatchability rate (the number of hatched eggs / number offertilized eggs), the survival rate (the number of early fish / amount of fish end), theperformance of growth (weight average transgenic F-3 / weight average non-transgenic) andfeed efficiency (absolute growth rate / daily feed intake). Results indicate that thetransmission rate ranged from 5-75% with an average transmission in the female transgenikF-2 by 2.5% (15/600) and 14.4% (75/520) in the male transgenik F-2. Weight growth rate of1.7 fold compared with non-transgenic. F-3 transgenic catfish has a degree of fertilization0.37% smaller and has a hatching 4% higher than the non-transgenic. Transgenic catfish feedefficiency higher than non-transgenic This shows that transgenic catfish has betterperformance compared with non-transgenic.Keywords: Transgenic catfish F-3, transgene (PhGH), transmission, Polymerase ChainReaction, growth performance
Co-Authors Bambang Iswanto BUDI UTOMO Darma Bakti Desrita Desrita Fery Jaksen Sihotang Imron Imron, Imron Indra Lesmana Putri Widyawati Sultan Akbar Habibullah Suprapto, Rommy Yunasfi Djayus Zulkifli Nasution