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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Jurnal Tumbuhan Obat Indonesia Biotropika Research Journal of Life Science Natural B Berkala Penelitian Hayati

Retno Mastuti

Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University

Author-ID : 3163823

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Effect of Banana Homogenate on Shoot Regeneration of Ciplukan (Physalis angulata L.) Varni Apensa; Retno Mastuti
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In vitro culture techniques can be used as an alternative problem solving in an effort to provide Ciplukan plant as a raw material of herbal medicine in a sustainable manner. The purpose of this study was to observe the effect of banana homogenate (BH) added into culture medium on shoot regeneration of Ciplukan (Physalis angulata L.) and plantlet development until acclimatization stage. The experimental design used was a randomized factorial group. The first factor was the two varieties of banana fruit namely, Ambon and Raja. The second factor was concentration of BH with four levels, 0 (control), 2.5%, 5% and 7.5 % and each treatment combination was repeated five times. Explant used in this research was the third node of 2 weeks old in vitro shoot after subculture to MS0 medium. Data analysis used ANOVA test. Eight week after culture (WAC) medium containing BH induced higher number of shoot compare to medium without BH. The addition of 5% BH var. Ambon had significant effect on increasing shoot number. Well rooted plantlets produced in MS0 rooting medium also showed high survival rate (66.7%< - 100%) two weeks after transferred to greenhouse. Therefore, the addition of BH into in vitro medium can be an alternative method for micropropagation of Ciplukan.Keywords: Banana homogenate, Ciplukan, micropropagation, nodal explants.

Anatomical Characters of Shoot Apical Meristem (SAM) on Bulbil Porang (Amorphophallus muelleri Blume) At the End of Dormancy Period Muhammad Naufal Afifi; Nunung Harijati; Retno Mastuti
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Porang is one species member of the genus Amorphophallus. In Indonesia, Porang is cultivated extensively because it has a high economic value. There is three seeded material available for planting. One of seeded material is bulbil. Bulbil is a vegetative reproductive organ of Porang plants which is located in the branch of the leaf. Bulbil has 3 - 4 months dormancy period. The dormancy period will end with shoot growth. Morphologically, there can be found a rough structure in the form of bulges in bulbil. The bulge is spread throughout the bulbil surface and is a growing area of shoots. Shoots on bulbil will grow through the bulge and only grow on the adaxial part, contrary shoots do not grow from the abaxial part. This study aims to compare the anatomy of shoot apical meristem (SAM) in the adaxial and abaxial parts of bulbil at the end of the dormancy period, ie 100 days after harvest (DAH), 110 DAH, 120 DAH, and 130 DAH. Anatomical observations were carried out by making semi-permanent preparations. The results showed that the anatomy of the adaxial and abaxial parts of bulbil Porang had differences in the development of SAM, leaf primordia, primary thickening meristem (PTM) and procambium.Keywords: Anatomy, Bulbil Porang, Dormancy, Shoot Apical Meristem.

Effect of Place and Time Storage on the Quality of Tubers Amorphophallus muelleri Blume Putri Rabiah Al Adawiah; Rodiyati Azrianingsih; Retno Mastuti
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Porang tubers are plant commodities that physiologically susceptible to damage. Longtime storage can decrease the quality of tubers. Furthermore, the presence of fungi or bacteria makes tubers cannot be stored in a long time. Decreasing quality of Porang tuber became a problem because of the higher demand for porang tubers. This research was conducted to determine the suitable place and time storage of porang tubers. Therefore, farmers can control or reduce the decrease in tuber quality. Methods of this research were an observation of color change based on the Munsell Color System, odor observation, hardness observation using penetrometer, the rotten area of tuber was measured using Leaf Area Meter, shrinkage of tuber weight, the percentage of rotten and sprouted tubers, density, and moisture content by the oven method. The results of this study were analysed statistically by ANOVA test. The hardness of the tubers has a significant effect on the storage place (soil, rack, and floor) for period 14 weeks. Storage time has an effect on changes of the weight loss, moisture content and decay area. The longer the tuber stored the higher percentage of sprouted and rotten tubers. The color of porang tubers change and the odor in the part of the rotten tubers increase during the storage period in each storage place. Porang tubers should not be stored for more than two weeks and placed on a rack to minimize the damage of the tubers.Keywords: Physical change, porang tubers, storage time.

Co-Authors Aminatun Munawarti Bina Rizki Amalia Budi Waluyo Edia Fitri Dwinianti Estri Laras Arumingtyas Estri Laras Arumingtyas Hiroshi Miyake Imaniah Bazlina Wardani Jati Batoro Muhammad Naufal Afifi Nunung Harijati Nunung Harijati Nunung Harijati Nunung Harijati Nunung Harijati Nunung Harijati Nurul Chairiyah Nurul Chairiyah Putri Rabiah Al Adawiah Rodiyati Azrianingsih Takeshi Taniguchi Varni Apensa Wahyu Widoretno Wahyu Widoretno Yoji Takeoka

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