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All Journal Jurnal Vektor Penyakit
Junus Widjaja
Balai Litbang Kesehatan Donggala, Badan Litbangkes, Kementerian Kesehatan RI
Environmental Science Health Professions Immunology & microbiology Public Health Veterinary

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Program Pengendalian Filariasis di Kabupaten Donggala, Sulawesi Tengah Made Agus Nurjana; Hayani Anastasia; Junus Widjaja; Yuyun Srikandi; Anis Nur Widayati; Murni Murni; Phetisya Pamela Frederika Sumolang; Ade Kurniawan; Mujiyanto Mujiyanto; Resmiwaty Resmiwaty
Jurnal Vektor Penyakit Vol 14 No 2 (2020): Edisi Desember
Publisher : Balai Penelitian dan Pengembangan Kesehatan Donggala, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/vektorp.v14i2.2512

Abstract

Abstract Donggala district had successfully conducted Transmission Assesment Survey–1 despite two children found positive for Brugia malayi. This study aimed to determine the progress of the filariasis program in Donggala District, Central Sulawesi. It was conducted in Kabonga Kecil, Banawa Sub-District and Sabang, Dampelas Sub-District from February to November 2017. Data collection included finger blood surveys, detection of Brugia malayi DNA, mosquito surveys, and in-depth interviews. From 638 people tested for finger blood survey, none of them were positive for microfilaria. Twenty children were tested for Brugia malayi DNA and the results were negative. A total of 2.978 mosquitoes were caught from mosquito surveys which identified as Aedes, Anopheles, Armigeres, Culex, Mansonia, Aedomyia, Uranotaenia, and Coquillettidia. PCR examination results showed all mosquito negative for Brugia malayi. A comprehensive and integrated surveillance strategy with other programs that are cost-effective and sustainable must continue to be carried out therefore the elimination of filariasis in the Donggala district can be achieved. Abstrak Kabupaten Donggala lulus Transmission Assesment Survey-1 meskipun masih ditemukan dua anak positif antibodi Brugia malayi. Penelitian ini bertujuan untuk mengetahui capain program filariasis di Kabupaten Donggala, Provinsi Sulawesi Tengah. Dilaksanakan di Kelurahan Kabonga Kecil, Kecamatan Banawa dan Desa Sabang, Kecamatan Dampelas pada bulan Februari – November 2017. Kegiatan meliputi survei darah jari, deteksi DNA Brugia malayi, survei nyamuk dan wawancara mendalam. Sebanyak 638 masyarakat diperiksa darahnya hasilnya seluruhnya negatif microfilaria. Sebanyak 20 anak diambil sampel darah untuk diperiksa deteksi DNA Brugia malayi hasilnya negatif. Nyamuk tertangkap sebanyak 2.978 nyamuk dari genus Aedes, Anopheles, Armigeres, Culex, Mansonia, Aedomyia, Uranotaenia, dan Coquillettidia. Hasil pemeriksaan PCR menunjukkan seluruh nyamuk negatif Brugia malayi. Strategi surveilans yang comprehensif dan terintegrasi dengan program lain yang cost-effective dan berkesinambungan harus terus dilakukan agar eliminasi filariasis di kabupaten Donggala dapat tercapai.
Leptospirosis pada Tikus di Kabupaten Minahasa, Provinsi Sulawesi Utara Tahun 2016 Leonardo Taruk Lobo; Meiske Elisabeth Koraag; Junus Widjaja; Arum Sih Joharina; Ayu Pradipta Pratiwi
Jurnal Vektor Penyakit Vol 14 No 2 (2020): Edisi Desember
Publisher : Balai Penelitian dan Pengembangan Kesehatan Donggala, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/vektorp.v14i2.3189

Abstract

Abstract Leptospirosis is an endemic zoonotic disease and remains a health problem in Indonesia. The word’s third-heighest cases of leptospirosis. This study aimed to determine the type of mouse as an intermediate transmission of leptospirosis disease in Minahasa District, North Sulawesi. Trapping of rats was performed by using 100 mouse traps (live Trap) for two consecutive days across six ecosystems. Rats were identified by external morphological characteristics. Microscopic Agglutination Test (MAT) and Polymerase Chain Reaction (PCR) were conducted using rat kidney samples. The number of rats caught in the present study was 105 heads consisting of six species e.g Rattus tanezumi, Rattus exulans, Rattus hoffmanni, Bunomis coelestis, Bunomis fratorum, and Paruromys dominator. The proportion of rats identified as MAT-based leptospirosis reservoir was 0.9% while the PCR test was 1.9%. Rattus tanezumi identified as leptospirosis reservoirs were found were found in the NHDP (non forest near to sattlement) ecosystem. Rattus tanezumi trapped near the settlement was positive as leptospirosis reservoirs in the Minahasa District. Abstrak Leptospirosis merupakan penyakit zoonosis endemik dan masih menjadi masalah Kesehatan di Indonesia. Angka CFR penyakit ini dilaporkan sebesar 2,5 – 16,4% atau rata-rata 7,1% sehingga menempatkan Indonesia sebagai negara peringkat ketiga tertinggi di dunia. Penelitian ini bertujuan untuk mengetahui jenis tikus perantara penyakit leptospirosis di Kabupaten Minahasa Provinsi Sulawesi Utara. Penangkapan tikus dilakukan dengan menggunakan 100 perangkap tikus (live trap) selama 2 hari berturut-turut di enam ekosistem. Tikus diidentifikasi dengan melihat karakteristik morfologi eksternalnya kemudian dilakukan pemeriksaan secara biomolekuler yaitu uji Microscopic Aglutination Test (MAT) dan Polimerase Chain Reaction (PCR) terhadap sampel ginjal tikus. Jumlah tikus yang tertangkap sebanyak 105 ekor yang terbagi menjadi enam spesies yaitu: Rattus tanezumi, Rattus Exulans, Rattus hoffmanni, Bunomis coelestis, Bunomis fratorum, dan Paruromys dominator. Proporsi tikus yang teridentifikasi sebagai reservoir leptospirosis berdasarkan uji MAT adalah 0,9% dan sebesar 1,9% berdasarkan uji PCR. Spesies Rattus tanezumi teridentifikasi sebagai reservoir leptospirosis dan ditemukan di ekosistem Non Hutan Dekat Pemukiman (NHDP). Beberapa Rattus tanezumi yang tertangkap di lokasi dekat pemukiman teridentifikasi positif sebagai reservoir leptospirosis di Kabupaten Minahasa.
Co-Authors Ade Kurniawan Anis Nur Widayati Arum Sih Joharina Ayu Pradipta Pratiwi Hayani Anastasia Leonardo Taruk Lobo Made Agus Nurjana Meiske Elisabeth Koraag Mujiyanto Mujiyanto Murni Murni Phetisya Pamela Frederika Sumolang Resmiwaty Resmiwaty Yuyun Srikandi