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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Akuakultur Indonesia
. Maskur
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Agriculture, Biological Sciences & Forestry

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Identifikasi Genetik Menggunakan Marker Mikrosatelit dan Hubungannya dengan Sifat Kuantitatif pada Sapi . Maskur; . Muladno; B Tappa
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.283 KB)

Abstract

The occurrence of genetic changes in Bali and Brangus cattle crossing and it's relationship to quantitative traits (e.g. growth and birth weight) were identified using microsatellite markers. Sixteen microsatellite loci were amplified using polymerase chain reaction (PCR) technique, the amplified products were then separated on vertical discontinuous polyacrylamide gel electrophoresis (PAGE) and silver-staining method was applied to detect the fragment. The evaluation to the stability and allele inheritance pattern were checked using Chi-Square segregation analysis. The results of this research showed that allele numbers average detected in Brangus and Bali cattle and their progeny were 2.31, 2.56, and 2.75 respectively. At most microsatellite loci, the allele distributions incline to concentrate and to form bimodal trend. Of 16 micro satellite loci tested, 5 microsatelitte markers namely INRA 037, HEL 9, CSSM 66, INRA 035 and ETH 225 indicated a significant response to average daily gain. In terms of birth weight, a significant response was shown by INRA 037. Key word: microsatellite, PCR, genotype, inheritance, allele
A Novel Single Nucleotide Polymorphism in Exon 4 of Insulin-Like Growth Factor-1 Associated with Production Traits in Bali Cattle . Maskur; C Arman; C Sumantri; E Gurnadi; . Muladno
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.748 KB) | DOI: 10.5398/medpet.2012.35.2.96

Abstract

Insulin-like growth factor-1 (IGF-1) is one of the gene candidates that can be used in selection strategy by using DNA markers (marker assisted selection). Gene candidate strategy is a molecular biology techniques to identify quantitative trait loci directly, with the assumption that genetic variation associated to quantitative trait variation. This study was designed to identify any new mutations in exon 4 that can cause the  IGF-1 gene polymorphism and then affect the production traits on Bali cattle. Single nucleotide polymorphism (SNP) discovery was conducted by using the direct sequencing technique. Genetic variation of the genes candidate was identified by using PCR-RFLP technique. The results of this study indicate the presence of a new SNP in exon 4 of IGF-1 gene caused by the T/C transition, which can be identified using Rsa1 restriction enzyme. Genotypic polymorphism of IGF-1/Rsa1 has a significant influence on birth weight, weaning weight and average daily gain of Bali cattle. CC genotype had a birth weight rate, weaning weight and average daily gain of: 15.64±1.83; 83.15±9.00, and 0.439±0.07 respectively, higher than the TT and CT genotype. IGF-1/Rsa1 can be used as a genetic marker for selection of birth weight, weaning weight, and daily body weight gain. 
Program for Fish Germ Plasm Conservation in Inland Waters Maskur, .
Jurnal Akuakultur Indonesia Vol. 1 No. 3 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.184 KB) | DOI: 10.19027/jai.1.139-143

Abstract

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Co-Authors B Tappa C Arman C Sumantri E Gurnadi