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Protein efficiency of hydrolyzed feather meal substitution in sheep ration Puastuti, Wisri; Mathius, I Wayan
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 3 (2007)
Publisher : Indonesian Animal Sciences Society

The use of protein ration in ruminant is affected by the characteristic of protein. The present study was carried out to evaluate the efficiency of protein of hydrolyzed feather meal (HBA) in ration for growing sheep. Twenty-five young rams of 7-8 months old with an average body weight of 21.16 Â± 2.47 kg were used in this study. This study was conducted for 12 weeks based on randomized block design (5x5). The control treatment (R0) contained 72% of total digestible nutrient (TDN) and 15% total crude protein (CP), whereas R1-R4 treatments were fed on improved ration containing 75% TDN and 18% CP where HBA was used to substitute feed protein and as by pass protein source. The HBA substitution was carried out in 5 levels, namely: R0 = grass + concentrate with 0% HBA; R1 = grass + concentrate with 1.1% HBA; R2 = grass + concentrate with 2.2% HBA; R3 = grass + concentrate with 4.4% HBA and R4 = grass + concentrate with 8.5% The result showed that an increase of CP in ration and HBA substitution might increase CP consumption (P<0.01) but CP digestibility were not affected. HBA substitution increased (P<0.01) the average daily gain (ADG) from 90.91 g/head (R0) to 125.33 g/head (R1-R4) or increased 37.86%, but it did not affect the efficiency of protein ration. The respons of HBA substitution levels in ration (x) to ADG followed the equation, ADG = 1.641x2 + 15.778x + 99.372 (r2 = 72.37%). It is concluded that 8.5% HBA substitution in ration can substitute 100% soybean meal, and 2.2% HBA substitution in ration gives the best efficiency to support daily gain. Key Words: Protein, Feather Meal, Substitution, Sheep

Cacao pod based ration supplemented with organic Zn: Growth responce on sheeps Puastuti, Wisri; Yulistiani, Dwi; Mathius, I Wayan; Giyai, Fransiscus; Dihansih, Elis
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Cacao pod can be used as feed to replace grass. The aims of study was to evaluate the growth response of sheep fed on cacao pod based rations to replace grass and supplemented with organic Zn. Fresh cacao pod was chopped to the size of 1-2 cm. In untreated cacao pod (without ammoniation), chopped cacao pod was dried then milled, while in ammoniated cacao pod, the chopped cacao pod was mixed with urea, 1.5% (w/w) and kept in anaerobic conditions for 7 days. After 7 days it was dried and milled. Both, without ammoniated and ammoniated cacao pod were used for ration formulation. The rations were formulated in iso protein and iso energy (16% CP and 70% TDN). The study used 25 male lambs Sumatera Composite breed at age 7-8 months old with average body weight 18.8 Â± 2.86 kg. Experiments were conducted using a randomized block design with number of animal in each group was 5. Five treatment rations evaluated were: R = grass + concentrate (positive control); K = cacao pod + concentrate; KZ = cacao pod + concentrate + organic Zn; KU = cacao pod ammoniated + concentrate; KUZ = cacao pod ammoniated + concentrate + organic Zn. The treatment rations were offered for 10 weeks. The results showed that the consumption of cacao pod based rations was higher (P < 0.05) than the grass-basal ration (75.19 g/kg BW0,75 vs 70.22 g/kg BW0,75). Suplementation of Zn organic to ammoniated or untreated cacao pod resulted in no significant difference in DM, and CP intake. DM, OM, CP digestibility and N retention were relatively similar among the fifth rations from 53.13 to 63.91%, from 54.69 to 66.49%, from 66.0 to 79.28% and from 9.5 to 13.4 g/h respectively. R ration had similar NH3 levels with KU ration (21.57 mM vs 22.94 mM) but higher than the K, KZ and KUZ ration (16.45 mM, 16.62 mM and 13.61 mM). VFA concentration showed no difference among treatments (from 140.32 to 179.76 mM). Sheep fed ration R had similar ADG with K and KZ (92.19 g/h vs 83.13 g/h; 93.44 g/h) but higher than the ration KU and KZ (73.44 g/h and 61.72 g/h). From this study can be concluded that grass-based rations can be replaced by the cacao pod based rations supplemented with organic Zn and resulted in relatively similar ADG of 92.19 g/h vs 93.44 g/h. Key Words: Cacao Pod, Ammoniation, Organic Zn, Sheep

Diacylglycerol Acyltransferase1 gene polymorphism and its association with milk fatty acid components in Holstein Friesian dairy cattle Asmarasari, Santi Ananda; Sumantri, Cece; Mathius, I Wayan; Anggraeni, Anneke
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 3 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v19i3.1078

Diacylglycerol acyltransferase 1 (DGAT1) gene is one of the major genes that has an important role in milk fat synthesis. This research was aimed at to identifying genetic polymorphism of the DGAT1 gene by PCR-RFLP method and its association to milk fatty acid components. Â Animals studied were Holstein Friesian (HF) cattle from BBPTU Baturraden (123 cows) and BPPT SP Cikole (36 cows). The length of PCR product of the DGAT1gene was 411 bp. Genotyping resulted in two types of alleles, namely K (411 bp) and A (203 and 208 bp); and two genotypes, namely KK (411 bp) and AK (203, 208 and 411 bp). For both locations, genotype frequency of AK (0.75) was higher than KK (0.25). The allele frequency of K (0.64) was higher than A (0.36). Heterozygosity of HF cattles at both locations was relatively high (Ho>He). The DGAT1 gene of the observed HF cattle was polymorphic. Result showed that there was an association between the DGAT1 polymorphism with unsaturated fatty acids especially in nervonat acid. The AK cows had a significant effect on unsaturated fatty acid content of which having a higher nervonat content (0.05%) (P<0.05) than that of the KK cows (0.03%). From the results, it is concluded that the DGAT1 gene can be functioned as a marker of selection for milk fatty acids. Key Words: DGAT1, PCR-RFLP, Holstein Friesian, Fatty Acids

Diacylglycerol Acyltransferase1 gene polymorphism and its association with milk fatty acid components in Holstein Friesian dairy cattle Santi Ananda Asmarasari; Cece Sumantri; I Wayan Mathius; Anneke Anggraeni
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 3 (2014): SEPTEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Diacylglycerol acyltransferase 1 (DGAT1) gene is one of the major genes that has an important role in milk fat synthesis. This research was aimed at to identifying genetic polymorphism of the DGAT1 gene by PCR-RFLP method and its association to milk fatty acid components. Animals studied were Holstein Friesian (HF) cattle from BBPTU Baturraden (123 cows) and BPPT SP Cikole (36 cows). The length of PCR product of the DGAT1gene was 411 bp. Genotyping resulted in two types of alleles, namely K (411 bp) and A (203 and 208 bp); and two genotypes, namely KK (411 bp) and AK (203, 208 and 411 bp). For both locations, genotype frequency of AK (0.75) was higher than KK (0.25). The allele frequency of K (0.64) was higher than A (0.36). Heterozygosity of HF cattles at both locations was relatively high (Ho>He). The DGAT1 gene of the observed HF cattle was polymorphic. Result showed that there was an association between the DGAT1 polymorphism with unsaturated fatty acids especially in nervonat acid. The AK cows had a significant effect on unsaturated fatty acid content of which having a higher nervonat content (0.05%) (P<0.05) than that of the KK cows (0.03%). From the results, it is concluded that the DGAT1 gene can be functioned as a marker of selection for milk fatty acids.

Cacao pod based ration supplemented with organic Zn: Growth responce on sheeps Wisri Puastuti; Dwi Yulistiani; I Wayan Mathius; Fransiscus Giyai; Elis Dihansih
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 4 (2010): DECEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cacao pod can be used as feed to replace grass. The aims of study was to evaluate the growth response of sheep fed on cacao pod based rations to replace grass and supplemented with organic Zn. Fresh cacao pod was chopped to the size of 1-2 cm. In untreated cacao pod (without ammoniation), chopped cacao pod was dried then milled, while in ammoniated cacao pod, the chopped cacao pod was mixed with urea, 1.5% (w/w) and kept in anaerobic conditions for 7 days. After 7 days it was dried and milled. Both, without ammoniated and ammoniated cacao pod were used for ration formulation. The rations were formulated in iso protein and iso energy (16% CP and 70% TDN). The study used 25 male lambs Sumatera Composite breed at age 7-8 months old with average body weight 18.8 ± 2.86 kg. Experiments were conducted using a randomized block design with number of animal in each group was 5. Five treatment rations evaluated were: R = grass + concentrate (positive control); K = cacao pod + concentrate; KZ = cacao pod + concentrate + organic Zn; KU = cacao pod ammoniated + concentrate; KUZ = cacao pod ammoniated + concentrate + organic Zn. The treatment rations were offered for 10 weeks. The results showed that the consumption of cacao pod based rations was higher (P < 0.05) than the grass-basal ration (75.19 g/kg BW0,75 vs 70.22 g/kg BW0,75). Suplementation of Zn organic to ammoniated or untreated cacao pod resulted in no significant difference in DM, and CP intake. DM, OM, CP digestibility and N retention were relatively similar among the fifth rations from 53.13 to 63.91%, from 54.69 to 66.49%, from 66.0 to 79.28% and from 9.5 to 13.4 g/h respectively. R ration had similar NH3 levels with KU ration (21.57 mM vs 22.94 mM) but higher than the K, KZ and KUZ ration (16.45 mM, 16.62 mM and 13.61 mM). VFA concentration showed no difference among treatments (from 140.32 to 179.76 mM). Sheep fed ration R had similar ADG with K and KZ (92.19 g/h vs 83.13 g/h; 93.44 g/h) but higher than the ration KU and KZ (73.44 g/h and 61.72 g/h). From this study can be concluded that grass-based rations can be replaced by the cacao pod based rations supplemented with organic Zn and resulted in relatively similar ADG of 92.19 g/h vs 93.44 g/h. Key Words: Cacao Pod, Ammoniation, Organic Zn, Sheep

Protein efficiency of hydrolyzed feather meal substitution in sheep ration Wisri Puastuti; I Wayan Mathius
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 3 (2007): SEPTEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The use of protein ration in ruminant is affected by the characteristic of protein. The present study was carried out to evaluate the efficiency of protein of hydrolyzed feather meal (HBA) in ration for growing sheep. Twenty-five young rams of 7-8 months old with an average body weight of 21.16 ± 2.47 kg were used in this study. This study was conducted for 12 weeks based on randomized block design (5x5). The control treatment (R0) contained 72% of total digestible nutrient (TDN) and 15% total crude protein (CP), whereas R1-R4 treatments were fed on improved ration containing 75% TDN and 18% CP where HBA was used to substitute feed protein and as by pass protein source. The HBA substitution was carried out in 5 levels, namely: R0 = grass + concentrate with 0% HBA; R1 = grass + concentrate with 1.1% HBA; R2 = grass + concentrate with 2.2% HBA; R3 = grass + concentrate with 4.4% HBA and R4 = grass + concentrate with 8.5% The result showed that an increase of CP in ration and HBA substitution might increase CP consumption (P<0.01) but CP digestibility were not affected. HBA substitution increased (P<0.01) the average daily gain (ADG) from 90.91 g/head (R0) to 125.33 g/head (R1-R4) or increased 37.86%, but it did not affect the efficiency of protein ration. The respons of HBA substitution levels in ration (x) to ADG followed the equation, ADG = 1.641x2 + 15.778x + 99.372 (r2 = 72.37%). It is concluded that 8.5% HBA substitution in ration can substitute 100% soybean meal, and 2.2% HBA substitution in ration gives the best efficiency to support daily gain. Key Words: Protein, Feather Meal, Substitution, Sheep

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