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Nurhayati Ansori Mattjik

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Author-ID : 317964

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance

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Articles

1

Improvement of Selected Induction Culture Media on Callus Induction in Anther Culture of Anthurium and a Histological Study on its Callus Formation Winarto, Budi; Mattjik, Nurhayati Ansori; Purwito, Agus; Marwoto, Budi
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Improvement of selected induction culture media on callus induction in anther culture of anthurium and a histologicalstudy on its callus formation were studied at the tissue culture laboratory of the Indonesian Ornamental CropsResearch Institute from February to October 2008. The objectives of the study were to optimize selected media forcallus formation, reveal cell origin of callus derived from anther culture and shoot formation process. Selectedmedia improved in the study were 1) MMS-TBN containing 0,5 mg/l TDZ, 1,0 mg/l BAP and 0,01 mg/l NAA (Winartomedium, WM) and 2) MMS III supplemented with 1,5 mg/l TDZ, 0,75 mg/l BAP and 0,02 mg/l NAA (Winarto andRachmawati medium, WRM). Improvement treatments were carried out by omission and application of 2,4-D in 0.5mg/l and reduction of medium strength of full, half, quarter, one eighth, one sixteenth, and zero strength. Afactorial experiment was arranged using a randomized complete block design with four replications. Results ofthis study indicated that the highest callus induction was clearly established in WRM. The medium stimulatedpotential growth of anther (PGA) up to 81% with 49% of percentage of anther regeneration (PAR) and 2.7 number ofcallus formed per replication (NCF). Significant improvement in callus formation was also recorded by reduction ofmedium strength of WRM to one eighth compared to others. The reduction induced PGA up to 58% with 29% of PARand 1.8 NCF. From histological studies it was well recognized that regenerated callus on half anthers cultured wasoriginated from middle layer cells of anther wall. The morphogenic response of anther wall cells caused primarilyon no androgenesis effect in microspore cells.

Zat Pengatur Tumbuh NAA (Naphtaleine Acetic Acid) dan BAP (6-Benzyl Amino Purine) serta Air Kelapa untuk Menginduksi Organogenesis Tanaman Anthurium (Anthurium andraeanum Linden Ex Andre) Prihatmanti, Dyah; Mattjik, Nurhayati Ansori
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 1 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

purpose of this experiment was to investigate the effect of plant growth regulators NAA, HAP, and coconut , water in Anthurium andraeaum propagation by tissue culture. The experimental design was factorial arranged in randomized block design. First factor was NAA concentration consisted of 0.0 mg/l, 0.1 mg/l, 0.2 mg/l. Second factor was HAP concentration consisted of 0 mg/l, 1 mg/l, 2 mg/l. Third factor was coconut water consisted of 0%. 10%, 20%, 30%. Response of survive explant was callus formation especially on NAA 0.2 mg/l. HAP 1 mg/l and 2 mg/l resulted greening callus followed by shoots formation. Coconut water 0, 10, and 20% could promote culture growth. The combination of treatment coconut water 0%, 10%, NAA 0.2 mg}J, and HAP 1 mg/l and 2 mg/l stimulated embriogenesis of callus to be shoots, leaves and roots. Key words : Anthurium andraeanum, NAA (Naphtaleine Acetic Acid), HAP (6-Henzyl Amino Purine)

Uji Adaptasi Somaklonal Kentang (Solanum tuberosum L.) Kultivar Introduksi Mattjik, Nurhayati Ansori; Muharmoko, Slamet
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 29 No. 3 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

One of The Asian Vegetable Research and Development Center programs is to get superior varieties potato. The experiment was objected to study somaclonal variety of several introduced potato varieties in order to select superior varieties from them. The plant height, the number of node, and petiole length of Atlantic cultivar were significant different from somaclonal cultivars, even though the number of stem and internode length were not different. However the number and weight of tuber Red Pontiac, Desiree, Russet Burbank, and Eba significant different from those somaclonal cultivars, but not the Atlantic cultivar. According to the principal component analysis for seven quantitative variables of the 34 plants numbers, there were two principal variables. The both variables I.e. variable 1 and 2, were able to explain about 79.3 % of the total variation. Therefore using the first and second variables may be explained the most of data variation. Somaclonal variation induced by irradiation were able to improve morfologis characters and yield, especially for Atlantic cultivar. Each of Atlantic and Red Pontiac somaclonal cultivar number had far relationship with their mother plants. Key words: Somaclonal, Potato

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