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Kadar Protein Klebsiella pneumoniae Hasil Pemanasan 65 Derajat Celcius Irawan Sugoro; Devita Tetriana
Al-Kauniyah: Jurnal Biologi Vol 7, No 1 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.149 KB) | DOI: 10.15408/kauniyah.v7i1.2712

Abstract

Klebsiella pneumoniae is one of a coliform bacteria that causing mastitis. This disease were founded in dairy cows and can be prevented by vaccination. The research has been conducted to determine the inactive times, the protein concentration and profile of K. pneumoniae which inactivated by heating of 65oC as material of mastitis vaccine. The cells culture inactivated by the different times, i.e. 0, 10, 20, 30, 40, 50 and 60 minutes. The inactive times was determined by the drop test method, whereas the protein concentration of cells were determined by Lowry method. The results showed that the inactive times occured after 30 minute, and has a significant different on the protein concentration of bacteria cells that inactivated by the different times.
Quality of Elephant Grass (Pennisetum purpureum Schumach.) Gamma Irradiation Result in the Second Generation (MV2) Manhalush Intan Shafifi; Dasumiati Dasumiati; Deudeu Lasmawati; Marina Yuniawati Maryono; Junaidi Junaidi; Irawan Sugoro
Al-Kauniyah: Jurnal Biologi Vol 15, No 1 (2022): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i1.17008

Abstract

AbstrakRumput gajah (Pennisetum purpureum Schumach.) memiliki kadar protein kasar 6,26%. Ternak ruminansia membutuhkan protein kasar 10–15%. Cara alternatif untuk meningkatkan kadar protein kasar dan karakter agronomi dapat dilakukan dengan metode mutasi induksi menggunakan sinar gama. Penelitian ini bertujuan untuk mengetahui kadar protein kasar tertinggi dan melakukan karakterisasi aksesi hasil iradiasi gama untuk proses seleksi. Rancangan percobaan yang digunakan, yaitu rancangan acak kelompok faktorial. Faktor pertama adalah iradiasi dosis dengan taraf 0, 10, 20, 30, 40, dan 50 Gy. Faktor kedua adalah buku batang, yaitu buku batang atas, tengah, dan bawah. Hasil penelitian menunjukkan bahwa protein kasar tertinggi terjadi pada perlakuan buku tengah dengan dosis 50 Gy dengan nilai 17,21% dan peningkatan persentase sebesar 12,19%. Hasil berpengaruh nyata pada generasi kedua terjadi pada karakter agronomi jumlah tunas. Kadar protein kasar tertinggi terjadi pada buku tengah dosis 50 Gy. Perlakuan buku atas dosis 10 Gy memperoleh karakter jumlah tunas yang banyak pada generasi kedua. Peningkatan kadar protein kasar dan karakter agronomi rumput gajah yang diperoleh belum stabil, karena termasuk dalam generasi awal. Hasil penelitian ini dapat digunakan sebagai penelitian lanjutan untuk memperoleh tanaman rumput gajah dengan kadar protein kasar dan karakter agronomi rumput gajah yang stabil.Abstract Elephant grass (Pennisetum purpureum Schumach.) had crude protein 6.26%. Ruminant animals need 10–15% crude protein. An alternative way to increase the crude protein with induction by used gamma-ray irradiation. The purpose of this research were to determine the highest crude protein content and characterize accessions resulting from gamma irradiation for the selection process. The experimental design used a random block design. The first factor, gamma irradiation with level dose 0, 10, 20, 30, 40, and 50 Gy. The second factor, upper node, middle node, and lower node. The result were the highest crude protein occurred in the middle node with a dosage of 50 Gy with a value of 17.21% and a percentage increase 12.19%. The significant result occurred in agronomic characters of total of shoots in the second generation. The highest increase of crude protein occurred on the middle node dose of 50 Gy. Interaction of the upper node dose of 10 Gy was able to obtain the most shoots in the second generation. The increase in crude protein content and agronomic character of elephant grass was not stable because it was included in the early generation. The results of this research can be used as further research to obtain elephant grass with stable high crude protein content and stable elephant grass agronomic characters.
PRODUKSI GAS METANA BATUBARA DENGAN PEMANFAATAN MIKROBA CAIRAN RUMEN PADA BERBAGAI PERLAKUAN KONDISI DAN MEDIA (Producing Methane Gas from Coal (CBM) by Utilizing Rumen Fluid Microbes in Various Media and Treatment Conditions) Dahrul Effendi; Bambang Agus Widjayanto; Kosasih Kosasih; Byan Muslim Pratama; Irawan Sugoro
Lembaran publikasi minyak dan gas bumi Vol 51 No 2 (2017)
Publisher : BBPMGB LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/LPMGB.51.2.17

Abstract

Produksi Gas metana Batubara (GMB) dapat ditingkatkan dengan cara biologi, yaitu melalui teknik biostimulasi dan bioaugmentasi yang dapat dilakukan secara in situ atau ex situ. Penelitian ini memanfaatkan mikroba cairan rumen yang diambil dari limbah rumah potong hewan. Cairan rumen mengandung konsorsium mikroba yang terdiri dari bakteri, protozoa dan fungi yang potensial dapat mendegradasi batubara untuk menghasilkan gas metana. Mikroba rumen memiliki kemampuan mencerna lignin dari tanaman yang merupakan materi asal mula batubara. Penelitian ini dilakukan dengan variasi konsentrasi campuran cairan rumen, jenis batubara, air formasi, suhu, dan tekanan. Hasil penelitian menunjukkan bahwa mikroba cairan rumen memiliki kemampuan untuk memproduksi gas metana, Volume gas tertinggi didapatkan dari batubara peringkat subbituminous pada suhu 60oC, tekanan 400 psi dan salinitas 35.000 ppm, yaitu diperoleh gas metana sejumlah 256 cf/ton dengan lama inkubasi 75 hari. Sedangkan pada batubara lignit dengan kondisi tekanan dan temperatur ruang (ambient condition), menghasilkan gas metana sebanyak 73,39 cf/ton dalam waktu 95 hari inkubasi. Produksi gas metana akan terus mengalami peningkatan sejalan dengan lama inkubasi dan tersedianya substrat batubara. Mikroba cairan rumen telah terbukti memiliki kemampuan untuk mendegradasi batubara menjadi gas metana sehingga potensi implementasinya dapat ditingkatkan dari skala laboratorium ke skala lapangan bawah permukaan untuk sumur CBM non-produktif. Production of Coalbed Methane (CBM) can be improved biologically, through biostimulation and bioaugmentation techniques which can be done by in situ or ex situ. This study utilizes microbial of the rumen fluid taken from animal slaughterhouse waste. Rumen fluid contains a microbial consortium of bacteria, protozoa and fungi that can potential to ferformace degradation of coal to produce the methane gas. Rumen microbes have the ability to digest lignin from plants that are the material of the origin of coal. This research was conducted with the variation of concentration of rumen fluid mixtures, coal type, water formation, temperature, and pressure. The results showed that rumen microbial fluid had the capability to produce methane gas. The highest gas volume was obtained from subbituminous coal rank at 60°C, 400 psi pressure and 35,000 ppm salinity, which produced methane gas volume of 256 cf / ton on 75 days incubation period. While from lignite coal of the ambient conditions was obtained methane gas volume as much as 73,39 cf / ton for 95 days of incubation time. The gas production will continue increasing in line with the incubation period and the availability of coal substrate. The rumen fluid microbes has been proven having capability to degradation of coal to producing methane gas so that potential implementation from laboratory scale to subsurface field scale for the non-productive CBM wells can be raised.