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Kadar Protein Klebsiella pneumoniae Hasil Pemanasan 65 Derajat Celcius Irawan Sugoro; Devita Tetriana
Al-Kauniyah: Jurnal Biologi Vol 7, No 1 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.149 KB) | DOI: 10.15408/kauniyah.v7i1.2712

Abstract

Klebsiella pneumoniae is one of a coliform bacteria that causing mastitis. This disease were founded in dairy cows and can be prevented by vaccination. The research has been conducted to determine the inactive times, the protein concentration and profile of K. pneumoniae which inactivated by heating of 65oC as material of mastitis vaccine. The cells culture inactivated by the different times, i.e. 0, 10, 20, 30, 40, 50 and 60 minutes. The inactive times was determined by the drop test method, whereas the protein concentration of cells were determined by Lowry method. The results showed that the inactive times occured after 30 minute, and has a significant different on the protein concentration of bacteria cells that inactivated by the different times.
Quality of Elephant Grass (Pennisetum purpureum Schumach.) Gamma Irradiation Result in the Second Generation (MV2) Manhalush Intan Shafifi; Dasumiati Dasumiati; Deudeu Lasmawati; Marina Yuniawati Maryono; Junaidi Junaidi; Irawan Sugoro
Al-Kauniyah: Jurnal Biologi Vol 15, No 1 (2022): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i1.17008

Abstract

AbstrakRumput gajah (Pennisetum purpureum Schumach.) memiliki kadar protein kasar 6,26%. Ternak ruminansia membutuhkan protein kasar 10–15%. Cara alternatif untuk meningkatkan kadar protein kasar dan karakter agronomi dapat dilakukan dengan metode mutasi induksi menggunakan sinar gama. Penelitian ini bertujuan untuk mengetahui kadar protein kasar tertinggi dan melakukan karakterisasi aksesi hasil iradiasi gama untuk proses seleksi. Rancangan percobaan yang digunakan, yaitu rancangan acak kelompok faktorial. Faktor pertama adalah iradiasi dosis dengan taraf 0, 10, 20, 30, 40, dan 50 Gy. Faktor kedua adalah buku batang, yaitu buku batang atas, tengah, dan bawah. Hasil penelitian menunjukkan bahwa protein kasar tertinggi terjadi pada perlakuan buku tengah dengan dosis 50 Gy dengan nilai 17,21% dan peningkatan persentase sebesar 12,19%. Hasil berpengaruh nyata pada generasi kedua terjadi pada karakter agronomi jumlah tunas. Kadar protein kasar tertinggi terjadi pada buku tengah dosis 50 Gy. Perlakuan buku atas dosis 10 Gy memperoleh karakter jumlah tunas yang banyak pada generasi kedua. Peningkatan kadar protein kasar dan karakter agronomi rumput gajah yang diperoleh belum stabil, karena termasuk dalam generasi awal. Hasil penelitian ini dapat digunakan sebagai penelitian lanjutan untuk memperoleh tanaman rumput gajah dengan kadar protein kasar dan karakter agronomi rumput gajah yang stabil.Abstract Elephant grass (Pennisetum purpureum Schumach.) had crude protein 6.26%. Ruminant animals need 10–15% crude protein. An alternative way to increase the crude protein with induction by used gamma-ray irradiation. The purpose of this research were to determine the highest crude protein content and characterize accessions resulting from gamma irradiation for the selection process. The experimental design used a random block design. The first factor, gamma irradiation with level dose 0, 10, 20, 30, 40, and 50 Gy. The second factor, upper node, middle node, and lower node. The result were the highest crude protein occurred in the middle node with a dosage of 50 Gy with a value of 17.21% and a percentage increase 12.19%. The significant result occurred in agronomic characters of total of shoots in the second generation. The highest increase of crude protein occurred on the middle node dose of 50 Gy. Interaction of the upper node dose of 10 Gy was able to obtain the most shoots in the second generation. The increase in crude protein content and agronomic character of elephant grass was not stable because it was included in the early generation. The results of this research can be used as further research to obtain elephant grass with stable high crude protein content and stable elephant grass agronomic characters.
PRODUKSI GAS METANA BATUBARA DENGAN PEMANFAATAN MIKROBA CAIRAN RUMEN PADA BERBAGAI PERLAKUAN KONDISI DAN MEDIA Dahrul Effendi; Bambang Agus Widjayanto; Kosasih; Byan Muslim Pratama; Irawan Sugoro
Lembaran Publikasi Minyak dan Gas Bumi Vol. 51 No. 2 (2017): LPMGB
Publisher : BBPMGB LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Produksi Gas metana Batubara (GMB) dapat ditingkatkan dengan cara biologi, yaitu melalui teknik biostimulasidan bioaugmentasi yang dapat dilakukan secara in situ atau ex situ. Penelitian ini memanfaatkan mikroba cairanrumen yang diambil dari limbah rumah potong hewan.