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All Journal Jurnal Natur Indonesia Biopropal Industri BERITA BIOLOGI Jurnal Pertanian Terpadu ANNALES BOGORIENSES Teknologi Indonesia Annales Bogorienses
Ruth Melliawati
LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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Journal : ANNALES BOGORIENSES

 1 
Glucoamylase Production by Aspergillus awamori KT-11 In Solid State Fermentation Using Cassava Peel as Substrate Perwitasari, Urip; Nuryati, Nuryati; Melliawati, Ruth; Yopi, Yopi
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.187 KB) | DOI: 10.14203/ab.v21i1.290

Abstract

In order to utilization of cassava peel waste this study tries to produce glucoamylase by solid state fermentation with Aspergillus awamori KT-11. Composition medium and drying technique are affecting the glucoamylase production. The highest glucoamylase activities were from cassava peel plus mineral medium. Activity glucomaylase in cassava peel plus mineral medium by oven drying was 365 U/mL and freeze dring was 452 U/mL.  It is conclud cassava peel plus mineral is a better substrate for glucoamylase production from A. awamori KT-11 in solid state fermentation. Powder of glucoamylase also proved capable of hydrolyzing starch-based biomass. 
Isolation of Endophytic Microbes from Gunung Halimun National Park, West Java, Indonesia and Bioassay Their Potency for Eradicating Microbial Crops Pathogen Sukara, Endang; Melliawati, Ruth
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/73

Abstract

Gunung Halimun National Park (GHNP) – West Java, Indonesia is the largest preserved primary forest in West Java. Diversity of plants and animals of this park have been studied intensively during the 15 years years. Diversity of endophytic microorganisms, however, has never been reported. Endophytic microbes are those who reside in the interspatial tissues of plants, have a capacity to produce array of secondary metabolites. This paper illustrates the occurrence of endophyte microbes in diverse flowering plants of GHNP. Total of 160 bacteria and 337 fungus were isolated from 86 plants species in the area. Out of that, 159 bacterial isolates and 320 fungus isolates survived in our collection and tested against four major microbial crops pathogen namely Xanthomonas campestris, Pseudomonas solanacearum, Colletotrichum glocosporoides and Fusarium oxysporum cubense. Plate Agar Test Assay method reveals that 51 among 159 bacterial isolates and 62 among 320 endophytic fungal isolates have an ability to inhibit the growth of microbial crop pathogens. Endophytic bacteria can inhibit more microbial crops pathogen but the inhibition ability is less compare to that of endophytic fungi. From our preliminary study clearly shows that endophytic microbes of GHNP should have potential value in developing biological control agent to combat microbial crop pathogens and eventually reduce the use of synthetic chemicals.
Enzyme Production From Cassava Peels by Aspergillus Awamori KT-11: The Making of Natural Sweetener From Several Tubbers Melliawati, Ruth; Rahman, Farida
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (565.264 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.20-29

Abstract

The use of cassava (Manihot esculenta Crantz) peel for enzyme production has not been widely used. The purpose of this study was to produce complex amylase enzymes from cassava peel by A. awamori KT-11 and apply them in the manufacture of natural sweeteners. Enzyme production is carried out on red and white cassava peel. Media of cassava peel sterilized, inoculated with 1%  A. awamori KT-11, incubated for 5 days, then dried at 50°C and mashed. Making sugar is done on cassava flour, sweet potato ( Ipomoea batatas L), taro (Colocasia esculenta) and cocoyam (Xanthosoma sagittifolium) with different concentrations of 10%, 15%, 20%, and 15% and 20% enzyme concentrations. The hydrolysis process is carried out for 3 days at 60°C. The enzyme activity in red cassava peel was 405,006 U/mL and white cassava peel was 321,239 U/ml. The sugar produced in cassava, taro, sweet potato, and Cocoyam was 101.38 mg/mL, 81.18 mg/mL, 55.929 mg/mL, and 42.874 mg/mL, respectively. The results of TLC showed that cassava and taro sugar  contain maltose, lactose and glucose, sweet potatoes contained glucose and dextrin and Cocoyam containing fructose. The sweetness level of sugar from cassava, taro, sweet potato and Cocoyam is 14 brix, 12 brix, 9 brix and 9 brix, respectively.
Co-Authors Apridah Cameliawati Djohan, Apridah Cameliawati Endang Sukara Harni, Harni Joko Purnomo NANIK RAHMANI Nuryati Nuryati Rahman, Farida Rohmattusolihat Rohmattusolihat, Rohmattusolihat Sulistiyowati, Eki Sunifah, Sunifah Urip Perwitasari, Urip YOPI YOPI