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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal

Mandojo Rukmo

Department Of Conservative Dentistry, Faculty Of Dental MEdicene, Universitas Airlangga

Author-ID : 3196153

Dentistry

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UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21 Qurni Restiani; Mandojo Rukmo; Devi Eka Juniarti
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

EKSPRESI TNF-α DAN CALCINEURIN PADA ASIMTOMATIS APIKAL PERIODONTITIS AKIBAT INDUKSI Enterococcus faecalis Randy Carlos Sietho; Mandojo Rukmo; Edhie Arif Prasetyo; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background. Gram positive bacteria strain are the major cause of endodontic failure as asymptomatic apical periodontitis. One of the dominant group of bacteria is Enterococcus faecalis that still persistent in root canal system post endodontic therapy procedures. This bacteria has lipoteichoic acid on its membrane that can cause induction of cytokines expression such as Tumor Necrosing Factor-α (TNF-α) and Calcineurin Purpose. This experiment to demonstrated asymptomatic apical periodontitis that induced with Enteroccus faecalis produce raising amount of TNF-α and Calcineurin expression cells in pericapical tissue of wistar rat. Method. The upper right molar teeth of the rat was drilled until perforation then exposed by BHIB 10µl (control positive group), E.faecalis 106 CFU in BHIB 10µl (experimental group) and without drilling (control negative group) then observed until 21th days and counting the amount of TNF-α and Calcineurin expression cells. Conclusion.The results show that asymtomatic apical periodontitis that was induced E.faecalis produce increasing amount of TNF-α and Calcineurin expression cells in periapical tissue wistar rat.

Antibakteri Ekstrak Kulit Buah Kakao (Theobroma cacao) 6,25% dan NaOCl 2,5% Terhadap Bakteri Streptococcus sanguinis Sepdhyo Wahyu Nugroho; Mandojo Rukmo; Edhie Arief Prasetyo; Tamara Yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Streptococcus sanguinis is a gram-positive bacterium that infects and penetrates into dentinal tubules from a depth of 150 μm up to 792 μm. Chemicals, namely 2.5% NaOCl, are used to mitigate the growth of these bacteria via irrigation of the root canals, but 2.5% NaOCl still has disadvantages including irritating periradicular tissue, having an unpleasant odor, and being toxic. Due to these shortcomings, natural materials are expected to be used as alternatives. Cocoa peel extract has active tannin compounds, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial attributes; a concentration of 6.25% is counted as the minimum bactericidal concentration (MBC) of Streptococcus sanguinis. Aim: To compare the difference of antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) and 2.5% NaOCl against Streptococcus sanguinis. Method: This research is an in vitro experimental laboratory with post-test only control group design. The diffusion method was used on Streptococcus sanguinis planted in tubes containing BHIB, then cultured on petri dishes containing nutrient agar and divided into 3 parts, namely 6.25% cocoa peel extract, 2.5% NaOCl, and negative controls, then each Petri dishes were given a paper disc that had been dripped by 0.01 ml of each ingredient, then incubated in an incubator for 2x24 hours at 37°C; the diameter of the inhibitory zone formed was then observed using a caliper. Results: The average diameter of the inhibition zone formed on 6.25% cocoa peel extract was 19.2000 mm and 2.5% NaOCl was 17.2813 mm against Streptococcus sanguinis. Conclusion: The antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) is higher than 2.5% NaOCl against Streptococcus sanguinis.

Antibiofilm activity of flavonoid mangosteen pericarp extract against porphyromonas gingivalis bacteria Aprillia Sonya Federika; Mandojo Rukmo; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background. Necrosis teeth need a procedure called the root canal treatment to eliminate microorganisms in root canal, so the inflammation process did not continue to the periapical.One of the microbes that exist in thenecrosis teethis Porphyromonas gingivalis, that capable of forming biofilm because it has the ability to communicate with bacteria signaling (quorum sensing). It is necessary to discover another antibiofilm agent as an irrigation material in root canal preparation deriving from nature. Flavonoidmangosteen pericarp extract can disrupt acyl-homoserine lactones (AHLs) thus quorum sensing bacteria will be disrupted and causing permanent attachment and the establishment of colonies on the surface of the teeth any barrier. Purpose. The aim of this study found out the antibiofilmactivity of flavonoid mangosteen pericarp extract against Porphyromonas gingivalis bacteria. Method. This studyhasa laboratory experimental with post-test only group design. Porphyromonas gingivalis ATCC 33277 was diluted according to Mc. Farland standard 106CFU/ml in Tryptic Soy Broth (TSB) medium and inserted into microtitterplate flexible U bottom. Porphyromonas gingivalis was incubatedfor 6x24 hour and checked with a simple staining to see the formation of biofilm. Flavonoid mangosteen pericarp extract was added in various concentrations and OD (optical density) readings done with a wavelength of 595 nm. Result. Flavonoid mangosteen pericarp extractshowed a decrease of OD value from control in all concentrations.Conclusion.Flavonoid mangosteen pericarp extract has antibiofilm activity against Porphyromonas gingivalis bacteria.

The effects of propylene glycol addition in the combination of calcium hydroxide and propolis on compressive and flexural strength Mandojo Rukmo; Nirawati Pribadi; Elisa Kusuma Wardani; Sukaton; Belinda Belinda Meilani Perdana Putri
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.32-37

Background: The mechanical properties of pulp-capping materials may affect their resistance to fracture during placement of a final restorative material or while supporting an overlying restoration over time when the mastication process is carried out. The combination of calcium hydroxide and propolis as a pulp capping material has the weakness on mechanical properties so it is necessary to add another material to improve its mechanical properties. Propylene glycol is used as a vehicle because it improves its handling property. Purpose: To explain how the compressive and flexural strength of the pulp capping material in the combination of calcium hydroxide, propolis, and propylene glycol compared without adding of propylene glycol. Methods: The study used 4 treatment groups with each group consisting of 7 replications. Control group is a combination of calcium hydroxide-propolis with a ratio of 1: 1.5, group 1 is a combination of calcium hydroxide-propolis-propylene glycol 20% with a ratio of 1:1.5:0.375, group 2 is a combination of calcium hydroxide-propolis-propylene glycol 30% with a ratio of 1:1.5:0.375, and group 3 is a combination of calcium hydroxide-propolis-propylene glycol 30% with a ratio of 1:1.5:0.375. Materials were mixed according to comparison and printed on a cylindrical mold with the size of 4 mm x 6 mm and a block mold with the size 65 mmx10 mmx6 mm. Then, the compressive and flexural strength was tested using an Autograph test instrument. Results: The statistical analysis was performed with ANOVA and Tukey HSD’s post-hoc test. There were statistically significant differences on compressive and flexural strength between groups (P<0.05). Conclusion: Addition of propylene glycol in the combination of calcium hydroxide and propolis have an influence of increasing of compressive and flexural strength, especially in the addition of 40% propylene glycol.

Co-Authors Adelina Kristanti Tandadjaja Afiono Agung Prasetyo Aprillia Sonya Federika Belinda Belinda Meilani Perdana Putri Deavita Dinari Devi Eka Juniarti Edhie Arief Prasetyo Edhie Arif Prasetyo Elisa Kusuma Wardani FX Ady Soesetijo, FX Ady Hendy Jaya Kurniawan Khilyat Ulin Nur Zaini Nirawati Pribadi Qurni Restiani Randy Carlos Sietho Ratna Puspita Hadi Risya Cilmiaty Risya Cilmiaty Rosita Rahmawati Sepdhyo Wahyu Nugroho Setyabudi Setyabudi Suhartono Taat Putra Sukaton, Sukaton Tamara Yuanita Uli Sasi Andari Widya Asmara

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