Articles
<![CDATA[The role of inducible Nitric Oxide Synthase in teeth periapical lesions immunopathogenesis caused by Enterococcus faecalis ]]>
<![CDATA[Tamara Yuanita]]>;
<![CDATA[Latief Mooduto]]>;
<![CDATA[Kuntaman Kuntaman]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 1 (2013): March 2013 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (198.797 KB)
|
DOI: 10.20473/j.djmkg.v46.i1.p14-17
<![CDATA[Background: Periapical lesions, are characterized by an immune response to the invading bacteria consequences periapical bone destruction. In root canal treatment failure was found Enterococcus faecalis (E. faecalis) as most species. iNOS found an important role in protection against infection, plays vital roles in fighting pathogens and contributing to disease pathology. Purpose: This study was to observed the role of iNOS in teeth periapical lessions immunopathogenesis caused by E. faecalis. Methods: The randomized post-only control group design used in this study, This study used 24 Wistar rats, were divided into three groups (each group consisted of 8 rats), as negative controls group is a normal teeth, in the positive controls group was made by drilling the upper right first molar to penetrate the dental pulp and was induced with 10µl BHI-b then filled with Glass Ionomer Cement (GIC) and the treatment group, after drilling the teeth, then inoculated with E. faecalis ATCC 29212 106 CFU into 10µl BHI-b then filled with GIC to prevent contamination. It takes 21 days to get periapical lesions and rat were sacrificed, and then the expression of iNOS was measured. Results: Statistical analysis using ANOVA found a significant differenced between control and treatment groups (p<0.05). Conclusion: This study concluded that iNOS role in teeth periapical lesions immunopathogenesis caused by E. faecalis.Latar belakang: Lesi periapikal merupakan hasil suatu respon imun untuk melawan invasi bakteri yang mengakibatkan destruksi tulang periapikal. Pada perawatan saluran akar yang mengalami kegagalan ditemukan Enterococcus faecalis sebagai spesies terbanyak. iNOS berperan penting untuk proteksi terhadap bakteri, mempunyai peran yang vital untuk melawan patogen dan berkonstribusi secara patologik untuk menyebabkan suatu penyakit. Tujuan: Penelitian ini bertujuan untuk mengobservasi peran iNOS secara imunohistokimia pada lesi periapikal tikus Wistar. Metode: Penelitian ini menggunakan disain randomized post-only control group, digunakan 24 ekor tikus Wistar yang dibagi menjadi tiga kelompok yang masing-masing terdiri dari 8 ekor tikus, sebagai kelompok kontrol negatif adalah gigi normal, pada kelompok kontrol positif dilakukan pengeboran pada gigi molar pertama rahang atas sampai menembus pulpa kemudian diinduksi 10µl BHI-b kemudian ditumpat (Glass Ionomer Cement) GIC dan pada kelompok perlakuan, setelah dilakukan pengeboran dilakukan induksi E. faecalis ATCC 29212 sebanyak 106 CFU ke dalam 10 µl BHI-b kemudian ditumpat GIC untuk mencegah kontaminasi. Diperlukan waktu 21 hari untuk mendapatkan lesi periapikal pasca perlakuan kemudian tikus dikorbankan lalu dihitung sel-sel yang mengekspresikan iNOS. Hasil: Analisis menggunakan ANAVA membuktikan bahwa ada perbedaan yang bermakna antara kelompok kontrol dan kelompok perlakuan (p<0,05). Kesimpulan: iNOS berperan pada imunopatogenesis lesi periapikal gigi akibat E. faecalis.]]>
<![CDATA[The cleanliness differences of root canal walls after irrigated with East Java propolis extract and sodium hypoclorite solutions ]]>
<![CDATA[Tamara Yuanita]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (680.264 KB)
|
DOI: 10.20473/j.djmkg.v50.i1.p6-9
<![CDATA[Background: Root canal instrumentation produces smear layer that covers dentine tubules of the root canal surface. Smear layer is organic and inorganic particles that have to be removed. East Java propolis extracts contais of saponin components used as a natural surfactant. 2.5% NaOCl and 5% NaOCl solutions have been widely used for irrigation in root canal treatment. Purpose: The purpose of this study was to analyze the cleanliness of the root canal walls, irrigated with aquadest, 8% East Java propolis extract, 2.5% NaOCl and 5% NaOCl. Method: Forty extracted teeth with straight single root canals were randomly divided into four groups(n=10). The specimens were prepared with ProTaper. During instrumentation, the root canals were irrigated with different solutions: Control Group irrigated with aquadest; Group 1 irrigated with 8% East Java propolis extract; Group 2 irrigated with 2.5% NaOCl and Group 3 irrigated with 5% NaOCl. The root canals were cut at apical third and SEM scores were tested by using Mann-Whitney test at the significance level of p=0.05 and Median Control test. Result: The results of Mann-Whitney Test, there were significant differences between control group with Group 1, 2 and 3 (p<0.05). Based on the Median Control test, the value of 8% East Java propolis extracts was 1,000, which was the best value compared to 2.5% NaOCl, 5% NaOCl and aquadest. Conclusion: It can be concluded that 8% East Java propolis extract is the most effective solution for cleaning root canal walls compared with 2.5% NaOCl and 5% NaOCl.]]>
<![CDATA[Cytoxicity test of NaOCl and Mangosteen (Garcinia Mangostin L.) peel extract used as an irrigation solution in human periodontal ligament fibroblast cells (HPdLFc) ]]>
<![CDATA[Tamara Yuanita]]>;
<![CDATA[Dina Ristyawati]]>;
<![CDATA[Karlina Samadi]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 3 (2018): September 2018 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (398.227 KB)
|
DOI: 10.20473/j.djmkg.v51.i3.p133-137
<![CDATA[Background: Root canal irrigation is an important stage in root canal treatment as it is requires to eliminate necrotic and debris tissue as well as root canal wetting. Unfortunately, root canal irrigation can cause the material utilised to pass into the apical foramen leading to periapical complications. Consequently, the irrigation solution should have low toxicity. Sodium hypochlorite (NaOCl) is a commonly used irrigation solution since it has antibacterial properties. Moreover, NaOCl is also known to have the ability to dissolve necrotic tissue, vital pulp tissue and organic components of dentin and biofilms. Nevertheless, it can still cause damage when coming into contact with periapical tissues. On the other hand, Mangosteen peel extract (Garcinia mangostana L.), also has antibacterial activities. Hence, Mangosteen peel extract is assumed to be employable as an alternative irrigation solution. Purpose: This research aimed to reveal the toxicity levels of NaOCl and Mangosteen peel extract (Garcinia mangostin L.) used as irrigation solution in human periodontal ligament fibroblast cells (HPdLFc). Methods: HPdLFc were obtained from periapical tissues taken from one third of the first premolar teeth cultured. These cells were subsequently divided into several groups exposed to NaOCl and Mangosteen peel extract at certain concentrations. A toxicity test was then conducted using MTT assay. The results were analyzed with an Elisa reader. Cell deaths and LC50 were then calculated. Results: NaOCl became toxic at a concentration of 0.254 µl/ml or 0.025%, while Mangosteen peel extract became so at one of 2.099 ug/ml or 0.209%. Conclusion: NaOCl can be toxic at a concentration of 0.254 µl/ml or 0.025% and Mangosteen peel extract at one of 2.099 μg/ml or 0.209%.]]>
<![CDATA[Contrasting efficacy of cocoa POD HUSK extract and 8% propolis extract in maintaining of root canal wall cleanliness ]]>
<![CDATA[Tamara Yuanita]]>;
<![CDATA[Uli Sasi Andari]]>;
<![CDATA[Mandojo Rukmo]]>;
<![CDATA[S. Sukaton]]>;
<![CDATA[Deavita Dinari]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 3 (2019): September 2019 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.20473/j.djmkg.v52.i3.p159-162
<![CDATA[Background: The existence of the smear layer, which can be produced during root canal instrumentation, may compromise the bond between filling material and the root canal walls. Therefore, the use of an effective root canal irrigation solution, a commonly employed form of which is sodium hypochloride (NaOCl), is important. Sodium hypochloride has several positive properties including effectiveness as a disinfectant agent and its ability to promote tissue-dissolution, although it is ineffective at cleaning the smear layer. There have been numerous recent studies of the application of phytomedicines in endodontics due to their advantages such as minimum toxicity and cost effectiveness. The saponin contained in both the propolis and cocoa pod husk acts as a surfactant that may lower surface tension and dissolve debris containing organic and anorganic materials. Purpose: The study aimed to provide evidence of the differences between root canal wall cleanliness when treated with 8% propolis extract and different concentrations of cocoa pod husk extract. Methods: 25 extracted teeth with single straight root canals were randomly divided into five categories (n=5). Sample preparation was performed using a rotary file and irrigated with different solutions. The first group was administered 2.5% NaOCl, the second group 8% propolis, the third group 3.12% cocoa pod husk extract, the fourth group 6.25% cocoa pod husk extract, and the fifth group 12.50% cocoa pod husk extract. The samples were then dissected into two sections at the apical third and their cleanliness scores subjected to a Mann-Whitney test with a significance level of p=0.05. Results: A significant difference was identified between all groups (p<0.05) and on the median control test, the highest value of 1.6 was recorded by the 6.25% cocoa pod husk extract, compared to the other four groups Conclusion: Cocoa pod husk extract demonstrates greater efficacy at cleaning root canal walls compared to 8% propolis extract.]]>
<![CDATA[Minimum inhibitory concentration of cocoa pod husk extract in Enterococcus faecalis extracellular polymeric substance biofilm thickness ]]>
<![CDATA[Tamara Yuanita]]>;
<![CDATA[Latief Mooduto]]>;
<![CDATA[Reinold Christian Lina]]>;
<![CDATA[Fajar Agus Muttaqin]]>;
<![CDATA[Ika Tangdan]]>;
<![CDATA[Revina Ester Iriani Marpaung]]>;
<![CDATA[Yulianti Kartini Sunur]]>
<![CDATA[ Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 4 (2019): December 2019 ]]>
Publisher : <![CDATA[Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.20473/j.djmkg.v52.i4.p215-218
<![CDATA[Background: Root canal treatment constitutes a treatment sequence for infected pulp to eliminate the etiological factors of pulp necrosis and periapical lesion. Enterococcus faecalis (E. faecalis) is an organism commonly found in a high proportion of root canal failure because of its ability to form biofilm. Degradation of extracellular polymeric substance (EPS) by oxidizing agents such as sodium hypochlorite is the first step in removing biofilm. However, the toxicity of sodium hypochlorite constitutes the main concern and, therefore, the safest alternative irrigants possible are required. The use of fruits, herbs and plants is widespread, especially in the fields of medicine and dentistry. Food crops are known to be rich in bioactive compounds, especially polyphenols, which have antioxidant and antimicrobial properties. Cocoa pod husk extract can, therefore, represent an alternative irrigant. Purpose: This study aimed to determine the minimum inhibitory concentration of cocoa pod husk extract in relation to the thickness of E. faecalis EPS biofilm. Methods: Four groups of E. faecalis cultured biofilm samples were analysed: group one contained E. faecalis without cocoa pod husk as a positive control; group two contained E. faecalis with 1.56% cocoa pod husk extract; group 3 contained E. faecalis with 3.125% cocoa pod husk extract; and group 4 contained E. faecalis with 6.25% cocoa pod husk extract. The biofilm thickness of all groups was measured by confocal laser scanning microscopy with statistical analysis subsequently undertaken by means of a post hoc test and Tukey HSD. Results: The average values of EPS biofilm thickness were as follows: group 1: 9500 nm; group 2: 8125 nm; group 3: 8000 nm; and group 4: 6375 nm. A post hoc Tukey HSD test indicated a significant difference between group 1 and group 4, while in group 2 and group 3 compared to group 1, there were no significant differences with the values of each being p = 0.340 and p = 0.267 (p>0.05). Conclusion: 6.25% cocoa pod husk extract reduces E. faecalis EPS biofilm thickness.]]>
<![CDATA[DAYA ANTI BAKTERI EKSTRAK KULIT NANAS (Ananas comosus) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis ANTIBACTERIAL POTENCY OF PINEAPPLE PEEL EXTRACT (Ananas comosus) ON Enterococcus faecalis GROWTH ]]>
<![CDATA[Rega Maurischa Arantika Putri]]>;
<![CDATA[Tamara Yuanita]]>;
<![CDATA[Mohammad Roelianto]]>
<![CDATA[ Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (267.736 KB)
|
DOI: 10.20473/cdj.v6i2.2016.61-65
<![CDATA[Background: Root canal infections is caused by the colonization of microorganisms. Microorganisms that commonly found in root canal failure is Enterococcus faecalis. These bacteria can be found in 80-90% of root canal infections. Currently, many plants are used as antibacterial drugs. Pineapple is one plant that can be used as antibacterial drugs. Pineapple peel has a variety of antibacterial chemical compounds, such as, bromelain, saponins, tannins and flavonoids that can inhibit the growth of Enterococcus faecalis bacteria. Aim: To obtain concentration of antibacterial from pineapple peel extract on Enterococcus faecalis growth. Methods: This study was an experimental laboratory with Post Test Only control group design using Enterococcus faecalis ATCC 29212 bacteria that were diluted based on Mc. Farland standard 1.5 x 108 CFU / ml with pineapple peel extract treatment concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0, 78% and then planted in nutrient agar media surface evenly. Results: At concentration of 3.125% pineapple peel extract showed that the growth of colonies was less than 90% of positive control and concentration of 6.25% pineapple peel extract had no visible Enterococcus faecalis bacteria growth as much as 99.9%. Conclusion: The pineapple peel extract (Ananas comosus) have antibacterial power on the growth of Enterococcus faecalis bacterial colonies with Minimal Inhibitory Concentration (MIC) of 3.125% and the Minimum Bactericidal Concentration (MBC) of 6.25%.]]>
<![CDATA[Perbedaan Kebocoran Tepi antara GIC Konvensional dan Resin Modified GIC pada Restorasi Kelas V (Difference on Microleakage of Conventional GIC and Resin Modified GIC in Class V Restoration) ]]>
<![CDATA[Aditya Rama Devara]]>;
<![CDATA[Cecilia G.J Lunardhi]]>;
<![CDATA[Tamara Yuanita]]>
<![CDATA[ Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (242.276 KB)
|
DOI: 10.20473/cdj.v6i2.2016.77-81
<![CDATA[Background. Microleakage is one of the challenging concerns in direct filling restorations. Restoration material should have good adaptation between the restoration and the cavity walls to seal the cavity in a good way. Glass ionomer cement (GIC) is one of restoration material which indicated for class V cavity. GIC has a good adherence to the cavity wall but fragile to liquid contamination during the setting time. Resin modified glass ionomer cement (RMGIC) is a hybrid glass ionomer cement with the addition of hidroxyethylmethacrylate (HEMA) in the liquid to increase the properties and endurance to liquid contamination. Aim. The aim of this study was to investigate the difference on microleakage of conventional GIC and resin modified GIC in class V restoration. Material and methods. Class V cavities (depth: 2 mm) were prepared on the cervical surface of 32 human first permanent premolars. Teeth were classified into three groups. Group 1: conventional GIC + varnish. Group 2:RMGIC + varnish. All cavities were restored, then stored in artificial saliva at 37ºC for 24 hours. The teeth were immersed in a 1% methylene blue dye solution for 24 hours, and then rinsed in running water, dried, and sectioned longitudinally. The section were assessed for microleakage of dye penetration by two independent evaluators using a digital microscope. Data were collected and statistically analyzed. Results. RMGIC showed no significant difference with conventional GIC. However, there is a slight difference, RMGIC has a slight lower microleakage than conventional GIC. Conclusion. RMGIC showed only slight lower microleakage than conventional GIC, but not significant.]]>
<![CDATA[EKSPRESI Interleukin-1 (IL-1) PADA PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis ]]>
<![CDATA[Yuliana Dwiwahyu Suryandari]]>;
<![CDATA[Ketut Suardita]]>;
<![CDATA[M. Mudjiono]]>;
<![CDATA[Tamara Yuanita]]>
<![CDATA[ Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (226.196 KB)
|
DOI: 10.20473/cdj.v7i2.2017.59-65
<![CDATA[Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-1. IL-1 is the proinflammation cytokine that is the key of host response bacteria invation and tissue damage. Also IL-1 could cause some indirectly tissue damage through the activation of MMPS. MMPs to stop the collagen formation. Purpose. The aim of this study is to know about the expression of IL- 1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of IL-1 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of IL-1 is increasing during the periapical tissue damage that induced by E.faecalis.]]>
<![CDATA[EKSPRESI Tumor Necrosis Factor alpha (TNF-α) DAN Transforming growth factors beta (TGF-β) PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI Enterococcus faecalis PADA TIKUS WISTAR ]]>
<![CDATA[Richard Fritzgerald]]>;
<![CDATA[Cecilia lunardhi]]>;
<![CDATA[Ruslan Effendy]]>;
<![CDATA[Tamara Yuanita]]>
<![CDATA[ Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (407.733 KB)
|
DOI: 10.20473/cdj.v7i2.2017.66-73
<![CDATA[Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation then lead to bone destruction. LTA stimulates immunology reaction that produce Tumor Necrosis Factor alpha (TNF-α) and Transforming growth factors beta (TGF-ß). TNF-α is a main mediator and also have an important role in inflamation response otherwise TGF-ß is working as a multifunction regulator of cell growth and differentiation during reforming and remodelling. Purpose. The aim of this study is to know about the expression of TNF-α and TGF-ß during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 30 male rats were randomly divided into 3 main groups, Group A (control negative) : normal tooth. Group B (control positive) : every tooth was induced only by sterile BHI-b. Group C (treated group) : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU). The animals were sacrificed 21 days later and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of TNF-α at treated group are higher than negative control and positive control but the expression of TGF-ß at treated group are higher than the negative control group but lower than positive control. Conclusion. From this study we know that the expression of TNF-α and TGF-ß are changing during the periapical tissue damage that induced by E.faecalis.]]>
<![CDATA[EKSPRESI TNF-α DAN CALCINEURIN PADA ASIMTOMATIS APIKAL PERIODONTITIS AKIBAT INDUKSI Enterococcus faecalis ]]>
<![CDATA[Randy Carlos Sietho]]>;
<![CDATA[Mandojo Rukmo]]>;
<![CDATA[Edhie Arif Prasetyo]]>;
<![CDATA[Tamara Yuanita]]>
<![CDATA[ Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (363.779 KB)
|
DOI: 10.20473/cdj.v7i2.2017.74-85
<![CDATA[Background. Gram positive bacteria strain are the major cause of endodontic failure as asymptomatic apical periodontitis. One of the dominant group of bacteria is Enterococcus faecalis that still persistent in root canal system post endodontic therapy procedures. This bacteria has lipoteichoic acid on its membrane that can cause induction of cytokines expression such as Tumor Necrosing Factor-α (TNF-α) and Calcineurin Purpose. This experiment to demonstrated asymptomatic apical periodontitis that induced with Enteroccus faecalis produce raising amount of TNF-α and Calcineurin expression cells in pericapical tissue of wistar rat. Method. The upper right molar teeth of the rat was drilled until perforation then exposed by BHIB 10µl (control positive group), E.faecalis 106 CFU in BHIB 10µl (experimental group) and without drilling (control negative group) then observed until 21th days and counting the amount of TNF-α and Calcineurin expression cells. Conclusion.The results show that asymtomatic apical periodontitis that was induced E.faecalis produce increasing amount of TNF-α and Calcineurin expression cells in periapical tissue wistar rat.]]>
