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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal

Setyabudi Setyabudi

Dental Medicine Faculty, Airlangga University, Surabaya

Author-ID : 682485

Dentistry

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Surface roughness of nanofilled and nanohybrid composite resins exposed to kretek cigarette smoke Laksmiari Setyowati; S. Setyabudi; Johanna Chandra
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 1 (2018): March 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Cigarette smoking is a public health issue that may influence the physical properties of dental composites. Surface roughness is one of the physical properties of restorative materials potentially influencing their success. The use of nanofilled and nanohybrid composites in dentistry has increased substantially over the past few years. Purpose: The purpose of this study was to evaluate the surface roughness of nanofilled and nanohybrid composite resins exposed to kretek cigarette smoke. Methods: Twelve cylindrical specimens of each material were prepared and divided into two groups (n=6). In the control groups, the specimens were immersed in distilled water for 24 hours at 37°C, with the water being renewed daily. For the experimental groups, the specimens were exposed to kretek cigarette smoke on a daily basis, then washed and soaked in distilled water at 37°C. After 21 days, the specimens were measured using a Surface Roughness Tester and the data was then statistically analyzed. Results: An Independent-T Test revealed that there were statistically significant differences in the surface roughness between the control and experimental groups of both nanofilled and nanohybrid composites, as well as between the nanofilled experimental group and the nanohybrid experimental group. Conclusion: Exposure to kretek cigarette smoke can increase the surface roughness of nanohybrid composites to a significantly greater extent than nanofilled composites.

Perbedaan Daya Antibakteri Antara Kombinasi Calcium Hydroxide-Based Sealer-Amoxicillin Dan Resin-Based Sealer-Amoxicillin Terhadap Bakteri Enterococcus Faecalis Asarizka Bena; Agus Subiwahjudi; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background. About 24-77% of root canal treatment failure cases are caused by infection of Enterococcus faecalis due to the resistance factor and virulence of these bacteria. Calcium hydroxide-based sealer and resin-based sealer are two types of sealer often used for root canal obturation due to their antibacterial properties. But the antibacterial properties owned by calcium hydroxide-based sealer has no effect on Enterococcus faecalis. Therefore, the adjunct of local antimicrobial such as amoxicillin is needed to increase antibacterial activities of root canal obturation materials (sealers) especially for calcium hydroxide-based sealer. Purpose. The aim of this study was to compare the effectiveness of antibacterial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin against Enterococcus faecalis. Methods. This study is an experimental laboratory with post test only control group design using Enterococcus faecalis ATCC 29212. Agar diffusion test was used to check the antimicrobial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin. by measuring the inhibition zone diameter of each treatment. Results. Resin-based sealer-amoxicillin’s diameter of zone inhibition is 37,3 mm and calcium hydroxide-based sealer-amoxicillin’s diameter is 18,7 mm. Conclusion. Antibacterial activity of resin-based sealer-amoxicillin is significantly greater than calcium hydroxide-based sealer-amoxicillin.

Perbedaan kekuatan geser resin komposit nanohybrid dengan teknik water-wet bonding dan ethanol-wet bonding terhadap dentin Fara Dwiyanti; Ruslan Effendy; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Composite resins are widely used today to replace lost tooth structure. Bonding between dentin and resin is a complex process that is influenced by several factors; one of which is ambient humidity. During drying after acid etching, it is recommended not to leave the surface of preparation too dry or too wet. If the surface is too dry, collagen will shrink and collapse. As a result, the bonding material cannot penetrate into the cavity between the fibers. If the dentine is too wet, the bonding material will also be difficult to bind with the collagen, either chemically or mechanically. The amount of water around the collagen closely correlates with the moisture created around the dentin surface during the drying process and also whether or not a rubber dam was used during the preparation process. This study is aimed to compare water wet bonding and ethanol wet bonding technique to control humidity. One way of measuring the strength of bonding is to measure shear strength. Purpose: to compare water wet-bonding and ethanol wet-bonding technique.Materials and Methods: 32 samples of bovine teeth incisors were randomly divided into 2 group. Each group consists of sixteen samples. Group 1 is treated with water wet-bonding,group 2 is treated with ethanol wet-bonding. Results: There is significant difference between group 1 and group 2. Conclusion: Shear bond strength of the composite resin bonding on dentin with ethanol wet-bonding technique is higher than the shear bond strength of the composite bonding on dentin with water wet-bonding.

Kekasaran Permukaan Resin Komposit Nanofilled dan Nanohybrid Setelah Paparan Asap Rokok Kretek Johanna Chandra; Laksmiari Setyowati; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Cigarette smoking is a public health problem that may influence physical properties of dental composites. Surface roughness is one of the physical properties of restorative materials that can influence their success. The use of nanofilled and nanohybrid composites in dentistry has substantially increased over the past few years. Purpose: The purpose of this study was to evaluate the surface roughness of nanofilled and nanohybrid composite resins exposed to kretek cigarette smoke. Methods: Twelve cylindrical specimens were prepared of each material and divided into two groups (n=6). For the control groups, the specimens were immersed in distilled water for 24 hours at 37oC and the water was renewed daily. For the experimental groups, the specimens were exposed daily to kretek cigarette smoke, then washed and stored in distilled water at 37oC. After 21 days, specimens were measured using a Surface Roughness Tester and the data was statistically analyzed. Result: Independent-T Test revealed that there were statistically significant differences in the surface roughness between control and experimental groups both nanofilled and nanohybrid, and between experimental groups nanofilled and nanohybrid. Conclusion: The exposure to kretek cigarette smoke can significantly increase the surface roughness of nanohybrid composites more than nanofilled composites.

Perbedaan Daya AntibakteriEkstrak Kulit Kokoa (Theobroma cacao)dan NaOCl 2,5% terhadapPorphyromonas gingivalis Tamara Yuanita; Rifatul Jannah; Edhie Arif Pasetyo; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Since pulp infection plays an important role in the development of periradicular lesions, endodontic treatment should be directed to eliminate bacterial and theirproducts. However, currently 20% of cases of apical periodontitis are not resolved after root canal treatment and therefore required for new root canal disinfection. The most commonly used irrigation material today is NaOCl 2.5%. However, NaOCl has negative effects, including being toxic when the material is injected into the periradicular tissue causing extensive pain, bleeding and swelling. Until now, many drugs come from plants that are still produced from plant extracts. One of the plants that can be utilized is cocoa (Theobroma cacao). Cocoa contains active compounds, such as saponins, tannins, alkaloids, flanonoids, aromatic terpenoids, theobromins and other metabolites. Cocoa husk has been studied to have an antibacterial effect on Porphyromonas gingivalis which is the main bacterial cause of apical periodontal. However, the difference in antibacterial activity between cocoa husk extract and NaOCl 2.5% againstPorphyromonas gingivalis has not been studied. Porpuse:The aim of this study is to compare antibacterial activity of cocoa husk extract and NaOCl 2.5% againstPorphyromonas gingivalis.Method: This research was a laboratory experimental study. Porphyromonas gingivalis were swabbed to nutrient agar medium. Consequently, cocoa husk extract 25% and NaOCl 2.5% were placed in wells of 5mm diameter and nutrient agar medium. The diameter of the zone of inhibition around the test materials was measured after 24 hours.Result:Cocoa husk extract has lower mean inhibitory zone diameter (14.22) than NaOCl 2.5% (16.06). Conclusion:Cocoa husk extract has lower antibacterial activity against Porphyromonas gingivalis compared to NaOCl 2.5%.

Compressive Strength Test on Calcium Hydroxide with Propolis Combination Ira Widjiastuti; S. Setyabudi; M. Mudjiono; Erika Setyowati
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide is a dental material used as a gold standard for pulp capping materials. However, calcium hydroxide has several weaknesses which cause many researchers to look for alternative ingredients that come from nature. Propolis in the field of dentistry has long been used because of its ability as an anti-inflammatory, anti-microbial, anti-fungal, and can cure scars. The combination of calcium hydroxide and propolis is proven to have good biocompatibility and anti-bacterial properties. One of the requirements of pulp capping material is to have sufficient compressive strength. Therefore, a research to test the compressive strength value of the combination of calcium hydroxide with propolis is needed. Purpose: To find out the difference in compressive strength of the combination of calcium hydroxide-propolis with a ratio of 1: 1, 1: 1.5, and 1: 2. Methods: The study used 4 treatment groups with each group consisting of 6 replications. Group 1 is a combination of calcium hydroxide-propolis with a ratio of 1: 1, group 2 with a ratio of 1: 1.5, group 3 with a ratio of 1: 2, and a positive control group using calcium hydroxide - sterile aquadest. Calcium hydroxide powder and propolis extract liquid is mixed according to comparison and printed on a cylindrical mold with the size of 4 mm x 6 mm. Then, the compressive strength was tested using an Autograph test instrument. Result: Compressive strength was smaller in group 3 compared to group 2, group 2 compared to group 1, and group 1 compared to the control group. Conclusion: In calcium hydroxide-propolis combination, the more propolis extract used in the combination the lower the compressive strength of the combination will be.

The effect of theobromine and NaF 2% exposure to enamel surface hardness after immersing in orange juice beverage Tamara Yuanita; Setyabudi Setyabudi; Qintan Sekar Adjani
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Consumption of orange juice which had acidic quality will cause a demineralization on enamel. The most effective way to prevent the demineralization process was by involving remineralization agents. Fluoride was effective to improve remineralization but has toxicity effect and caused fluorosis at certain dose. Another alternative for remineralization agent without providing side effects is theobromine contained in cocoa peel extract (Theobroma cacao). Objective: To explain the effect of theobromine and NaF 2% exposure on enamel surface hardness after immersion in commercial orange juice. Method: Twenty-seven cattle incisors were cut into a square shape 1x1 cm and then planted in a round resin mold then divided into three groups. All three groups were immersed in orange juice beverage for 60 minutes. The control group was immersed in distilled water, group I was given theobromine 200 mg / L, and group II was given 2% NaF after exposure for 96 minutes. Surface hardness measurements were carried out using the Wolpert Micro Vickers Testers tool. Results: There was a significant difference (p <0.05) between surface hardness of tooth enamel from the control group and theobromine group and there was also a significant difference between the control group and the NaF group. However, there was no significant difference (p> 0.05) in the NaF group with theobromine group. Conclusion: The effect of exposure to theobromine and NaF 2% on surface enamel hardness after immersion in orange juice beverage has the same result.

Effect of Hydrogel Epigallocatechin-3-Gallate (EGCG) to the Number of Fibroblast Cell Proliferation in the Perforation of Wistar Rat Tooth Pulp Ira Widjiastuti; Setyabudi setyabudi; Kun Ismiyatin; Djoko Agus Purwanto; Tiara Sukmawati
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Pulpitis can occur because the deep cavity preparation and it causes increasing of NO levels. Perforated teeth require direct pulp capping (DPC) treatment. The current standard DPC material is calcium hydroxide. However, several studies have found weaknesses of calcium hydroxide that can affect the success of DPC treatment and new, more biocompatible materials are needed. Epigallocatechin-3-gallate (EGCG) in green tea has many benefits, including antioxidant, anticolagenase, anticancer, anti-inflammatory and has the ability of radical scavenging to clean NO so that pulp healing can occur better by increasing the number of fibroblast cells that play a role in wound healing. Purpose: To determine the concentration of hydrogel EGCGs that are effective in increasing the number of fibroblast cell proliferation in the dental pulp perforation of Wistar rats. Method: This research is a laboratory experimental study with a randomized post test only control group design. Samples used in the study were 24 male Wistar rats which were divided into four groups, namely the negative control group and the treatment group were given EGCG 60 ppm, 90 ppm, and 120 ppm and were decapitated on the 7th day after treatment. The maxilla and the 1st molar were taken and decalcified, to process the HPA reading with HE staining. Observations were made using a microscope with a magnification of 400x. Results: There were significant differences in the treatment groups with 60 ppm and 90 ppm hydrogel hydrogels on the results of the Oneway ANOVA difference test (p <0.05). Conclusion: The concentration of hydrogel EGCG which is effective in increasing fibroblast cell proliferation is 90 ppm.

Antibiofilm activity of flavonoid mangosteen pericarp extract against porphyromonas gingivalis bacteria Aprillia Sonya Federika; Mandojo Rukmo; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background. Necrosis teeth need a procedure called the root canal treatment to eliminate microorganisms in root canal, so the inflammation process did not continue to the periapical.One of the microbes that exist in thenecrosis teethis Porphyromonas gingivalis, that capable of forming biofilm because it has the ability to communicate with bacteria signaling (quorum sensing). It is necessary to discover another antibiofilm agent as an irrigation material in root canal preparation deriving from nature. Flavonoidmangosteen pericarp extract can disrupt acyl-homoserine lactones (AHLs) thus quorum sensing bacteria will be disrupted and causing permanent attachment and the establishment of colonies on the surface of the teeth any barrier. Purpose. The aim of this study found out the antibiofilmactivity of flavonoid mangosteen pericarp extract against Porphyromonas gingivalis bacteria. Method. This studyhasa laboratory experimental with post-test only group design. Porphyromonas gingivalis ATCC 33277 was diluted according to Mc. Farland standard 106CFU/ml in Tryptic Soy Broth (TSB) medium and inserted into microtitterplate flexible U bottom. Porphyromonas gingivalis was incubatedfor 6x24 hour and checked with a simple staining to see the formation of biofilm. Flavonoid mangosteen pericarp extract was added in various concentrations and OD (optical density) readings done with a wavelength of 595 nm. Result. Flavonoid mangosteen pericarp extractshowed a decrease of OD value from control in all concentrations.Conclusion.Flavonoid mangosteen pericarp extract has antibiofilm activity against Porphyromonas gingivalis bacteria.

THE EFFECT OF CALCIUM HYDROXIDE-PROPOLIS ON THE NUMBER OF MACROFAG CELLS ON THE WISTAR RAT DENTAL WITH PULPA PERFORMANCE Setyabudi Setyabudi; Devi Eka Juniarti; Ira Widjiastuti; Maughfirah Shintya Fathori
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.58-61

Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Objective: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of wistar rats with pulp perforation. Method: This study used 30 samples of Wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwithcention. Macrophage cell is calculated histopathologically by using compound light microscope on the 3rd day with 400x magnification. Results: The Kruskal-Wallis test results showed significant differences in macrophages after the combination of calcium hydroxide and propolis (p = 0,000). The combined application of calcium hydroxide and propolis showed higher macrophages than the application of calcium hydroxide and the control group. Conclusion: Application of calcium hydroxide - propolis combination was proven increase the number of macrophage cells in Wistar Rats (Rattus Norvegicus) with pulp perforation

Co-Authors Agung Sosiawan Agus Subiwahjudi Ahmad Yudianto Andi Syahrimayani Aprillia Sonya Federika Arif Rahman Nurdianto Arif Rahman Nurdianto Asarizka Bena Devi Eka Juniarti Dian Agustin Wahjuningrum Djoko Agus Purwanto Dwikora Novembri Utomo Edhie Arif Pasetyo Erika Setyowati Fara Dwiyanti Fery Setiawan Heni Puspitasari Ira Widjiastuti Johanna Chandra Johanna Chandra Kun Ismiyatin Laksmiari Setyowati Laksmiari Setyowati M. Mudjiono Mandojo Rukmo Maughfirah Shintya Fathori Nadira Nurin Febrianti Nia Nur Haliza Qintan Sekar Adjani Ria Chusnita Rifatul Jannah Ruslan Effendy Sri Kunarti Sukaton, Sukaton Swadheena Patro Tamara Yuanita Tasya Nafatila Chalisha Tiara Sukmawati Virdah Dwi Dewaantari Yuline Krishartini

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