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All Journal Agroland : Jurnal Ilmu-Ilmu Pertanian AGRISAINS Proceeding Buffalo International Conference Agroland: Jurnal Ilmu-ilmu Pertanian Mitra Sains
Mirajuddin Mirajuddin
Universitas Tadulako
Agriculture, Biological Sciences & Forestry Education

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Journal : AGRISAINS

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ANGKA KEBUNTINGAN KAMBING LOKAL PALU HASIL IB_PERTAMA MENGGUNAKAN SPERMA CAIR SUPLEMENTASI Leucocephala TANNIN Mirajuddin, Mirajuddin
AgriSains Vol 12, No 2 (2011)
Publisher : FAPETKAN UNTAD

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Abstract

An experiment on the conception rates of local Palu goat artificially inseminated with sperms that were prepared in vitro with supplementation of tannin from Leucocephala with             a glucose extender was done in Palu and Sigi District, Central Sulawesi from June to November 2009. The sperm donors were 2 Ettawa Crosses aged about 2.5 years from which the sperms were collected with artificial vagina. Normal ejaculates (based on microscopic as well as macroscopic analyses) were prepared into liquid sperms suitable for artificial insemination (AI). A diluted sperm with 18.9% glucose extender served as the control (K0) and this was directly (day 0 after dilution) used to inseminate 19 PGF2α-synchronized goats. For treatment group, a dlituted sperm was supplemented with Leucocephala tannin (2.5% per ml sperms) and was used to inseminate 22 female goats after being frozen for 14 d at 14°C. Spermatozoa concentration for the two preparations was similar  (200 x 106 cell/ml/AI). It was found that the conception rate for the control group was 73.68% while for the treatment group was 95.45%.Key words : Artificial Insemination (AI), conception rate, Leucocephala Tannin, local goat Palu, sperm preparation.
PENENTUAN WAKTU IB TANPA SINKRONISASI ESTRUS PERIODE POSTPARTUM BERDASARKAN PROFIL HORMON ESTROGEN DAN LUTEINIZING HORMONE (LH) PADA KAMBING LOKAL Mirajuddin, Mirajuddin
AgriSains Vol 13, No 2 (2012)
Publisher : FAPETKAN UNTAD

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Research on “determination of AI timewithout estrus synchronization in post partum period beased on estrogen hormone profile and LH in local goat ", is part of the National Strategic Research Grant, which is funded by the University Tadulako in Fiscal Year 2010. The research was done at the Laboratory of Physiology and Reproduction, Faculty of Animal Science, University of Gajah Mada (UGM), Integrated Research and Testing Laboratory  UGM, Laboratory of Obsterics, Department of  Animal Reproductive Medicine, University of Airlangga,  group of Goat farmers in the city of Palu. This study aimed to find solutions to the failure of the AI implementation Central Sulawesi, due to the limitations of frozen sperm, liquid Nitrogen, and the expensiveness of estrus synchronization hormon. Post partum local goatwas used as experimental animal. Blood was taken for  analysis  estrogen and LH hormones  (immunometric assay) in one estrous cycle, namely in 36 -55 days after giving birth. Implementation of the AI (without estru synchronization) was based on the time and the highest concentration of estrogen (1000 ρg / ml, as treatment 1), the concentration of LH surge (160 ηg / ml: treatment 2), and on the first day after the LH surge (as treatment 3), while the implementation of the AI with estrus synchronization was as the control (treatment 0). The AI implementation was done by using liquid sperm of Etawah cros-bred goat  that were prepared with 2.5% tannin (leucocephala) supplementation per ml sperm. Conception rate (conception rate: CR) of AI was calculated based on the percentage of non pregnant femalesd (nonreturn Rate: NRR) of total females artificially inseminated, where the treatment 1 was 20%, 73.3% (treatment 2), 60% (treatment 3), and Control of  86.6%.Key words: Estrogen hormon and LH, Postpartum, AI, estrus synchronization, and local goat.
Co-Authors Danus Danus Fatmawati Saloko, Fatmawati M. Hamsun, M. Mochammad Imron Awalludin Nelly Kusrianty Yohan Rusiyanto Yunober Mberato