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Aktivitas Antibakteri Kitosan dari Tulang Rawan Cumi-Cumi (Loligo sp.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Laode Muhamad Hazairin Nadia; La Ode Huli; Waode Nilda Arifiana Effendy; Frets Jonas Rieuwpassa; Imra Imra; Nurhikma Nurhikma; Eko Cahyono
Jurnal FishtecH Vol 10, No 2 (2021)
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/fishtech.v10i2.14386

Abstract

Squid (Loligo sp.) is one of fisheries commodities export in Indonesia. Squid has cartilage that can be utilized in the food and non-food sectors. The inner skin of the squid cartilage contains chemical compounds are chitin and chitosan. The objectives of this research are to produce chitosan from squid cartilage and tested the antibacterial effectiveness of chitosan in bacteria Staphylococcus aureus and Escherichia coli. The research method was carried out in twophase; the manufacturing phase of chitosan from squid cartilage and the testing phase for the antibacterial activity of chitosan. The results of the first phase of this study indicate that the chitosan produced meets the quality standards of chitosan with water content 7.82% (bk), ash content 0.57% (bk), nitrogen content 3.18% (bk) and degree of deacetylation 87.43%. Further more the results obtained in the second phase showed that the higher of chitosan concentration, the greater of inhibition zone in the tested bacteria. The best antibacterial activity was found at 0.8% chitosan concentration, with an inhibition zone of 12.8 ± 0.06 mm in E. coli and 11.1 ± 0.12 mm in S. aureus. At a concentration of 0.8% showed a greater inhibitory value when compared to 70% alcohol.
Analisis Organoleptik dan B-Karoten Nugget Ikan Nila (Oreochromis sp.) dengan Penambahan Tepung Wortel (Daucus carota L) Waode Nilda Arifiana Effendy; Laode Muhamad Hazairin Nadia; Sri Rejeki; La Ode Huli
Jurnal FishtecH Vol 11, No 1 (2022)
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/fishtech.v11i1.15812

Abstract

Generally, nuggets on the market are made from chicken meat but today the price of chicken meat is high so it needs alternative raw materials in making nuggets. One of the raw materials that can be used for making nuggets is tilapia meat. Tilapia has high protein and a soft texture. To increase the attractiveness and nutritional value of nuggets, so vegetables can be added. Carrot is one of the vegetables that can be used as an added ingredient in tilapia fish nuggets because it contains ?-carotene which is a source of ?-tocopherol, dietary fiber, ascorbic acid, tocopherol, and natural antioxidants. The purpose of this study was to determine the effect of adding carrot flour with different concentrations to the organoleptic values and levels of ?-carotene of selected tilapia fish nuggets. This study uses a quantitative research method with a Completely Randomized Design (CRD) model with 4 treatment levels and 3 replications. The treatment in this study refers to the concentration of carrot flour were K0 (0%), K1 (8%), K2 (10%), and K3 (12%). Parameters observed were organoleptic (color, flavor, taste, and texture) and ?-carotene. The results showed that the addition of carrot flour had a significant effect on the organoleptic value of tilapia nuggets (p<0.05). The best treatment in this study was the addition of 12% carrot flour concentration (K3) with panelists acceptance rate at color 7.83 (like), flavor 7,96 (like), taste 7,52 (like) and texture 7,60 (like). K3 treatment was the selected tilapia nugget with the value of ?-carotene 12.09 ppm (bk) and 24.03 ppm (bb).
KARAKTERISTIK FISIK SURIMI IKAN NILA (Oreochromis sp. ) BERDASARKAN PERBEDAAN PROSES PENCUCIAN MENGGUNAKAN NaHCO3 DAN NaCl Laode Muhamad Hazairin Nadia; Illian Elvira; La Ode Huli; Wa Ode Nafilawati; Sri Rejeki; Waode Nilda Arifiana Effendy
Jurnal Fish Protech Vol 5, No 2 (2022): Jurnal Fish Protech Vol. 5 No. 2 Oktober 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55679/jfp.v5i2.28303

Abstract

Penelitian ini bertujuan untuk mengetahui karakteristik fisik surimi ikan nila (Oreochromis sp.) dengan perbedaan proses pencucian menggunakan NaHCO3 dan NaCl. Penelitian ini menggunakan metode penelitian kuantitatif dengan model Rancangan Acak Lengkap (RAL) satu faktor perlakuan yaitu pencucian lumatan daging ikan nila yang terdiri dari   4 taraf perlakuan dan masing - masing dilakukan 3 kali pengulangan, perlakuannya yaitu perlakuan A (kontrol atau tanpa NaHCO3 dan NaCl),perlakuan B (NaHCO30,4% dan NaCl 0,3%), perlakuan C (NaHCO30,5% dan NaCl 0,3%) dan perlakuanD (NaHCO30,6% dan NaCl 0,3%). Parameter fisik yang diuji yaitu rendemen, derajat putih, Water Holding Capacity (WHC), uji lipat (folding test) dan uji gigit (teeth cutting test). Hasil penelitian menunjukkan bahwa pencucian dengan NaHCO3dan NaCl berpengaruh nyata terhadap nilai parameter fisik (kekuatan gel, derajat putih, WHC, uji lipat dan uji gigit) surimi ikan nila (p<0,05). Hasil rendemen tertinggi surimi ikan nila pada perlakuanD sebesar (34,98%), sedangkan terendah pada perlakuan A (33,13%). Hasil pengukuran dari parameter fisik menunjukkan bahwa perlakuan D sebagai perlakuan terbaik dengankekuatan gel 690,24 g.cm, derajat putih 66,94%, WHC 68,46%, uji lipat 4,89 dan uji gigit 8,75. Kata Kunci: ikan nila, karakteristik fisik, NaCl, NaHCO3, surimi
Application of Chitosan as Chlorine Substitution in Tilapia Fillet (Oreochromis sp.) Laode Muhamad Hazairin Nadia; Frets Jonas Rieuwpassa; Agustina Agustina; Reni Tri Cahyani; Wulandari Wulandari
Jurnal FishtecH Vol 11, No 2 (2022)
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/fishtech.v11i2.17178

Abstract

Chitosan has gained high interest in recent times to extend the shelf-life of fish product due to its nontoxicity, biodegradability, biocompatible and antibacterial activity. This research was aimed to determine the antibacterial effect of chitosan on the shelf-life of tilapia fillets at room temperature storage (25-27 oC). This study used a actorial randomized design (RAF) with two factors, namely antibacterial treatment consisting of 3 levels (positive control with distilled water immersion on tilapia fillets, immersion of tilapia fillets with 0.8% chitosan and negative control with 10 ppm chlorine immersion in tilapia fillet) and observation time consisted of 3 levels (0 hours, 6 hours and 12 hours observations). Antibacterial treatment consisted of  3 levels, namely. Each treatment was carried out in three replications. The data were analyzed using analysis of variance (ANOVA) and the duncan multiple range test (DMRT) at a 95% confidence level. The results of the research showed that chitosan was able to suppress bacterial growth and suppress the increase in TVB values at room temperature storage for 12 hours, indicated by the significant difference in the total number of bacteria and TVB values with chlorine and controls (p<0.05). These indicated that chitosan has a function as a natural antibacterial to replace chlorine in the shelf-life extension of tilapia fillets.
Application Of Foam-Mat Drying Technology On Production Of Indo-Pacific Tarpon (Megalops cyprinoides) Flavor Enhancer Reni Tri Cahyani; Sarita Elda Maudi; Hasmini Hasmini; Laode Muhamad Hazairin Nadia
Jambura Fish Processing Journal Vol 5, No 1 (2023): VOLUME 5 NOMOR 1, JANUARI 2023
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jfpj.v5i1.14053

Abstract

Fish have the potential to be a natural source of flavor. Hence, they can be used as the main ingredient in flavor enhancers. This research examines the characteristics of flavor enhancers made from Indo-pacific tarpon produced using foam-mat drying technology. The formulations were the ratio between the weight of fish fleshes and additives namely 30:70 (A); 40:60 (B); 50:50 (C); 60:40 (D); and 70:30 (E). The test parameters included yield, pH, Maillard reaction product, sensory (preference) and proximate value. The best formulation was based on proximate value. The results showed that the different formulations significantly affected the yield, pH, and Maillard reaction product (p0,05). The lower the fish flesh used in the formulation, the higher the yield and Maillard reaction product, while the pH decreased. The effectiveness index test based on sensory value (preference) showed that the formulation D (60:40) was the best formulation that contained water of 8.49 ± 0.30%; ash of 33.03 ± 0.10%; protein of 29.67 ± 0.26%; total fat of 2.00 ± 0.15% and carbohydrates of 26.74 ± 0.10%.
KARAKTERISTIK ORGANOLEPTIK DAN KIMIA SOSIS BERBASIS SURIMI IKAN NILA (Oreochromis sp. ) DENGAN PENAMBAHAN ISOLAT PROTEIN KEDELAI Laode Muhamad Hazairin Nadia; La Ode Huli; Reni Tri Cahyani; Wila Rumina Nento
Jurnal Fish Protech Vol 6, No 1 (2023): Jurnal Fish Protech Vol. 6 No. 1 April 2023
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v6i1.29196

Abstract

ABSTRACTThis study aims to determine the concentration of the selected soy protein isolate (SPI) emulsifying agent (the most preferred by panelists) and chemical characteristics of tilapia surimi sausage. This study used a quantitative research method with a completely randomized design (CRD) model with one treatment factor, namely the addition of soy protein isolate (GPA), which consisted of 4 levels of treatment, each of which was repeated 3 times, the treatment was treatment A (control or without the addition of SPI), treatment B (12% SPI addition), C treatment (13% SPI addition) and D treatment (14% SPI addition). The organoleptic parameters tested were appearance, color, aroma, taste and texture, while the chemical parameters were emulsion test and proximate analysis (water content, ash, protein and fat). The results showed that the sausages were selected based on the hedonic test or preference for adding soy protein isolate concentration (GPA) of 12% with an appearance parameter value of 7.18 (like), color of 7.06 (like), taste of 7.38 (like). ), aroma of 6.48 (rather like) and texture of 7.02 (like). Sausages with the addition of 12% GPA concentration had chemical characteristics in the form of emulsion stability of 82.18% and proximate analysis of water, ash, protein and fat content respectively of 60.40%, 0.61%, 15.97% and 1 .60% (fulfills SNI 7755:2013). Keywords: soy protein isolate, organoleptic, tilapia surimi, sausageABSTRAKPenelitian ini bertujuan untuk menentukan konsentrasi bahan pengemulsi  isolat protein kedelai (IPK) terpilih (yang paling disukai panelis) dan karakteristik  kimia dari sosis surimi ikan nila. Penelitian ini menggunakan metode penelitian kuantitatif dengan model Rancangan Acak Lengkap (RAL) satu faktor perlakuan yaitu penambahan isolat protein kedelai (IPK) yang terdiri dari 4 taraf perlakuan masing- masing dilakukan 3 kali pengulangan, perlakuannya yaitu perlakuan A (kontrol atau tanpa penambahan IPK), perlakuan B (penambahan IPK 12%) perlakuan C (penambahan IPK 13%) dan perlakuan D (penambahan IPK 14%). Parameter organoleptik yang diuji yaitu kenampakan, warna, aroma, rasa dan tekstur, sedangkan parameter kimia yaitu uji emulsi dan analisis proksimat (kadar air, abu, protein dan lemak). Hasil penelitian menunjukkan bahwa sosis terpilih berdasarkan uji hedonik atau kesukaan penambahan konsentrasi isolat protein kedelai (IPK) sebesar 12% dengan nilai parameter kenampakan sebesar 7,18 (suka), warna sebesar 7,06 (suka), rasa sebesar 7,38 (suka), aroma sebesar 6,48 (agak suka) dan tekstur sebesar 7,02 (suka). Sosis dengan penambahan konsentrasi IPK 12% memiliki karakteristik kimia berupa stabilitas emulsi sebesar 82,18% dan analisis proksimat kadar air, abu, protein dan lemak secara berturut-turut sebesar 60,40%, 0,61%, 15,97% dan 1,60% (memenuhi SNI 7755:2013). Kata Kunci: isolat protein kedelai, organoleptik, surimi ikan nila, sosis
Karakteristik Kimia dan Profil Asam Lemak Ikan Nila Merah (Oreochromis sp.) dari Umur Panen Berbeda Laode Muhamad Hazairin Nadia; Wulandari Wulandari; Yunialdi Hapynes Teffu; Fatur Rahman Rustan; La Ode Abdul Fajar Hasidu
Jurnal FishtecH Vol 12, No 1 (2023)
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/fishtech.v12i1.20056

Abstract

Red tilapia is in demand by the public as a consumption fish because it has several advantages, namely thick flesh and high nutritional value. This study aims to determine the chemical characteristics and fatty acid profile of red tilapia with harvesting ages of 16, 20 and 24 weeks. This research method used a completely randomized design (CRD) and each carried out 3 replications. The analysis used is the actorial pattern with one factor (16, 20 and 24 week harvesting age treatment). Data were analyzed using Analysis of Variance (ANOVA) and Duncan Multiple Range Test (DMRT) at 95% confidence level. The results showed that the water and protein content increased with increasing harvesting age for red tilapia, while the ash and fat content decreased. The highest water and protein content at 24 weeks of harvest were 80.17% and 17.85%, respectively. Fatty acids in red tilapia consist of 4 SAFA, 1 MUFA and 3 PUFA. The highest fatty acid content of red tilapia, namely palmitic fatty acid, was 27.26% at harvest age of 24 weeks and increased with increasing harvest age.