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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Pendidikan Biologi Undiksha Jurnal Penelitian Pendidikan IPA (JPPIPA)
Ni Putu Dian Pertiwi
Universitas Pendidikan Ganesha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Materials Science & Nanotechnology Physics

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Identifikasi Spesies Ikan Pelagis yang Dijual di Pasar Kota Denpasar Menggunakan Marka Control Region Mitokondria (mtDNA) Ni Putu Dian Pertiwi
Jurnal Pendidikan Biologi Undiksha Vol. 9 No. 1 (2022)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Identification and inventarisation of fish species is important for fisheries data. Information regarding fish species sold within the local markets of Denpasar city-Bali have not been collected and written as a good data, especially identification using molecular methods and comparison between local name and species name. Therefore, this study aims to identify species of pelagic fish sold within markets of Denpasar city using control region mitochondrial (mtDNA) marker. A total of 7 samples of pelagic fish were collected from 2 markets within Denpasar city, in the form of full body fish, fish that has been boiled, and boiled fish body parts. DNA samples were amplified at the control region of mtDNA, and obtained the sequence length of 508-527 bp. Identification was conducted using BLAST methods and phylogenetic analysis. The result showed that the samples were identified as 4 species, i.e. Auxis rochei, Auxis thazard, Thunnus albacares, and Thunnus obesus. Through this study, we also revealed that fish with the local name “ikan tongkol” was the species of Auxis rochei, Auxis thazard, Thunnus albacares, meanwhile fish known as the local name “ikan cakalang” was the species of Thunnus obesus. The results of this study was expected to be a preliminary study about the diversity of fish sold within markets in Denpasar city, and also as the additional genetic data for pelagic fish within Bali waters. This will also act as the additional information for the methods of fish species identification.
Population Analysis of Bali Sardinella (Sardinella lemuru, Bleeker 1853) Landed in PPI Kedonganan using Length-Weight Data and Digital Analysis (ImageJ) Ni Putu Dian Pertiwi; Ida Ayu Purnama Bestari; M. Danie Al Malik; Mark Anthony Phuong
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 1 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.1.90-96

Abstract

Sardinella lemuru, one of the important pelagic fish in Bali Strait waters, has been reported to be in the overfishing condition. Various studies have been conducted to understand its population condition. Length-weight data has been also widely used to understand the population dynamic of S. lemuru, although data collection generally using manual direct measurement methods, which has the disadvantages if used on the enormous number of samples. Therefore, this research aims to understand the significance of S. lemuru length measurement using digital image analysis (ImageJ) compared with the manual measurement. Moreover, this research also aims to understand the population condition of S. lemuru landed in PPI Kedonganan analyzed using its length-weight data. The result indicated no significance difference between length measurement using manual methods and digital image methods, thus conclude that digital image data can be used to measure the fish length and represent the accurate measurement. Population condition analysis showed that S. lemuru population is in isometric condition (b=3); with indication of declining in the length growth pattern compared with previous research (L∞= 20.75 cm with K= 1.1); the mortality estimation showed that fishing mortality (F=1.27) was lower than natural mortality (M=2.22), with the survival rate higher on smaller size fish. This research result can be used as an addition to the S. lemuru fisheries data and can also be used as reference methods to help conduct sampling and data collection more efficiently.
Differences in the Percentage of Fusarium Wilt Disease Attacks and High Vegetative Growth of Purple Eggplant (Solanum melongena L.) Plants with Various Concentrations of Antagonist Fungus Trichoderma harzianum Ketut Srie Marhaeni Julyasih; I Putu Parwata; Ni Putu Dian Pertiwi
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.12090

Abstract

Microorganisms that have antagonistic properties against pathogens are an alternative as a material for control, such as Trichoderma sp. The use of biological agents has the potential to control plant diseases safely and environmentally friendly, Trichoderma sp is a fungus that can be a biocontrol agent because it is antagonistic to other fungi. Trichoderma sp is a fungus whose habitat is in the soil, including the Ascomycetes class which has green spores. This fungus has the potential for degradation of various heterogeneous substrates in the soil, positive interactions with the host, producing enzymes to improve plant nutrition. Until now, there has not been much research conducted on the competence of Trichoderma sp. isolates in inhibiting the development of Fusarium wilt disease. The purpose of this study was to test the difference in the percentage of Fusarium wilt disease attacks on eggplant plants and plant vegetative growth due to the provision of variations in the concentration of Trichoderma sp. The type of research conducted was a true experimental, with the research design used was a Completely Randomized Design using 6 treatments, namely with the treatment of Trichoderma sp. isolate concentration. grown on rice media with concentrations of 0%, 10%, 20%, 30% 40%, and 50%, each treatment was repeated 5 times so that there were 30 experimental units. The variables observed in this study were the differences in the percentage of Fusarium Wilt disease attacks and the high vegetative growth of purple eggplant plants. The data obtained were analyzed using the analysis of variance of the ANOVA test with a level of 5%. If there is a significant difference, it is continued with the Duncan test at a level of 5%. The results showed that there was a significant difference in the variation of T. harzianum concentration on the percentage of Fusarium wilt disease attacks, and the high growth of purple eggplant plants.
Co-Authors I Putu Parwata Ida Ayu Purnama Bestari Ketut Srie Marhaeni Julyasih M. Danie Al Malik Mark Anthony Phuong