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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Jurnal Pendidikan Biologi Undiksha Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Abdi Insani Journal of Marine and Coastal Science Jurnal Perikanan

Ketut Srie Marhaeni Julyasih

Universitas Pendidikan Ganesha

Author-ID : 386015

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics

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VARIASI DOSIS TEPUNG CANGKANG TELUR AYAM MENINGKATKAN JUMLAH DAUN DAN BERAT KERING TANAMAN KANGKUNG DARAT (Ipomoea reptans Poir var. mahar) Rosita Putri, Ni Putu Ulan; Julyasih, Ketut Srie Marhaeni; Ratna Dewi, NP Sri
Jurnal Pendidikan Biologi undiksha Vol 6, No 3 (2019)
Publisher : Undiksha

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Limbah cangkang telur ayam mengandung unsur hara yang baik untuk tanaman namun belum dimanfaatkan secara optimal di bidang pertanian. Penelitian ini bertujuan untuk mengetahui: (1) Perbedaan jumlah daun dan berat kering tanaman kangkung darat setelah diberikan tepung cangkang telur dengan variasi dosis yang berbeda, (2) Dosis tepung cangkang telur ayam yang paling efektif untuk meningkatkan jumlah daun dan berat kering tanaman kangkung darat). Penelitian ini adalah penelitian eksperimen sungguhan (true experimental research). Variasi dosis tepung cangkang telur ayam yang digunakan dalam penelitian ini adalah 0gr, 10gr, 15gr, 20gr, dan 25gr. Rancangan dasar yang digunakan yaitu Rancangan Acak Lengkap (RAL). Desain penelitian yang digunakan dalam penelitian ini adalah simple random sampling. Populasi dalam penelitian ini adalah tanaman kangkung darat yang telah tumbuh pada saat di semai yang berasal dari satu kemasan biji kangkung darat varietas Mahar. Sampel yang digunakan adalah tanaman kangkung darat yang telah berumur 7 hari sebanyak 30 tanaman. Data dianalisis dengan ANOVA satu arah dan uji lanjutÂ Beda Nyata Terkecil dengan taraf signifikansi 5%. Hasil penelitian ini adalah: (1) terdapat perbedaan jumlah daun dan berat kering Â tanaman kangkung darat setelah diberikan tepung cangkang telur ayam dengan variasi dosis yang berbeda. Berdasarkan dari hasil uji hipotesis bahwa angka signifikansi, p<0.05 artinya data berbeda bermakna dan 2) Hasil Beda Nyata Terkecil menunjukkan dosis tepung cangkang telur ayam 25 gram yang paling efektif untuk meningkatkan berat kering Â tanaman kangkung darat.

ISOLASI DAN IDENTIFIKASI JAMUR MIKROSKOPIS PADA RIZOSFER TANAMAN JERUK SIAM (Citrus nobilis Lour.) DI KECAMATAN KINTAMANI, BALI Ristiari, Ni Putu Nila; Julyasih, Ketut Srie Marhaeni; Suryanti, Ida Ayu Putu
Jurnal Pendidikan Biologi undiksha Vol 6, No 1 (2019)
Publisher : Undiksha

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Penelitian ini bertujuan untuk mengetahui genus jamur mikroskopis yang terdapat pada rizosfer tanaman jeruk siam (Citrus nobilis Lour.) di Kecamatan Kintamani, Bali. Penelitian mengacu pada pendekatan penelitian kualitatif dengan jenis penelitian yaitu penelitian deskriptif. Subjek pada penelitian ini adalah seluruh jamur mikroskopis pada rizosfer tanaman jeruk siam di perkebunan jeruk siam di Desa Kintamani. Adapun objek pada penelitian adalah genus jamur mikroskopis pada rizosfer tanaman jeruk siam yang diisolasi dari salah satu perkebunan jeruk siam di Desa Kintamani. Tahapan penelitian terdiri dari tahap persiapan, pelaksanaan, dan analisis data. Hasil penelitian yakni melalui pengamatan makroskopis dan mikroskopis diperoleh 12 isolat diantaranya berasal dari 4 genus yaitu Aspergillus (4 isolat), Penicillium (3 isolat), Mucor (1 isolat), dan Trichoderma (4 isolat). Berdasarkan hasil tersebut, dapat disimpulkan yaitu 12 isolat jamur mikroskopis yang ditemukan, memiliki karakteristik makroskopis dan mikroskopis yang berbeda-beda.

VARIASI KOMPOSISI MEDIA TANAM ARANG SEKAM, TANAH KOMPOS DAN PASIR TERHADAP BERAT KERING TANAMAN KANGKUNG DARAT (Ipomoea reptans Poir var. bangkok) ., NI MADE DWI RIANI SARASWATI; ., Dr. Ir. Ketut Srie Marhaeni Julyasih, M.; ., Ni Putu Sri Ratna Dewi, S.Pd., M.Pd.
Jurnal Pendidikan Biologi undiksha Vol 4, No 2 (2017)
Publisher : Undiksha

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Penelitian ini bertujuan untuk mengetahui: (1) Perbedaan berat kering tanaman kangkung darat (Ipomoea reptans Poir var. bangkok) dengan pemberian variasi komposisi media tanam arang sekam, tanah kompos dan pasir., (2) Komposisi media tanam yang paling efektif terhadap berat kering tanaman kangkung darat (Ipomoea reptans Poir var. bangkok). Penelitian ini menggunkan eksperimen sungguhan (true experimental). Variasi komposisi media tanam arang sekam, tanah kompos dan pasir yang digunakan dalam penelitian ini adalah kontrol (tanah) M0, 1:1:1 (M1), 1:1:2 (M2), 1:2:1 (M3), 1:2:2 (M4), 2:1:1 (M5), 2:2:1 (M6), 2:1:2 (M7) dengan 3 ulangan. Rancangan dasar yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL). Teknik pengambilan sampel yang digunakan dalam penelitian ini adalah simple random sampling. Populasi dalam penelitian ini adalah tanaman kangkung darat yang sudah tumbuh pada saat disemai yang berasal dari satu indukan biji kangkung darat (Ipomoea reptans Poir var. bangkok). Sampel yang digunakan adalah tanaman kangkung darat yang telah berumur 7 hari sebanyak 24 sampel. Data dianalisis dengan menggunakan ANOVA satu arah dan uji lanjut Beda Nyata Terkecil (BNT) dengan taraf signifikansi 5%. Hasil penelitian ini adalah: (1) Terdapat perbedaan berat kering tanaman kangkung darat setelah diberikan variasi komposisi media tanam arang sekam, tanah kompos dan pasir dengan komposisi yang berbeda., 2) Pemberian variasi komposisi media tanam arang sekam, tanah kompos dan pasir pada perlakuan M6 yang paling efektif untuk meningkatkan berat kering tanaman kangkung darat. Kata Kunci : arang sekam, tanah kompos, pasir, kangkung darat (Ipomoea reptans Poir var. bangkok), berat kering. This study aims to find out: (1) differences in dry weight of Ipomoea reptans Poir var. bangkok by giving variations in the composition of husk charcoal, compost and sand planting media, (2) the most effective planting media composition for dry weight Ipomoea reptans Poir var. bangkok plant. This research used true experimental. The variations in the composition of the husk charcoal, compost and sand planting media used in this study were control (soil) M0, 1:1:1 (M1), 1:1:2 (M2), 1:2:1 (M3), 1:2:2 (M4), 2:1:1 (M5), 2:2:1 (M6), 2:1:2 (M7) with 3 replication. The basic design used in this study was Complete Random Design (CRD). The sampling technique used in this study was simple random sampling. The population in this study were Ipomoea reptans Poir var. bangkok plants that had been grown at the time of sowing which came from one parent of Ipomoea reptans Poir var. bangkok seeds. The samples used were Ipomoea reptans Poir var. bangkok plants which were 7 days old as many as 24 samples. Data were analyzed using one-way ANOVA and the smallest significant difference (LSD) further test with a significance level of 5%. The results of this study were: (1) there were differences in dry weight of Ipomoea reptans Poir var. bangkok after a variety of compositions of husk charcoal, compost and sand with different compositions are given., 2) giving variations in the composition of the husk charcoal, compost soil and sand planting media in the M6 treatment with the ratio of composition 2: 2: 1 is the most effective for increase the dry weight of Ipomoea reptans Poir var. bangkok.keyword : husk charcoal, compost soil, sand, Ipomoea reptans Poir var. bangkok, dry weight.

ISOLASI DAN IDENTIFIKASI JAMUR ENDOFIT PADA TANAMAN JERUK SIAM (Citrus nobilis) DI DESA KINTAMANI BANGLI BALI ., Monika Megawati Ferdiana Dara; ., Dr. I Wayan Sukra Warpala,S.Pd., M.Sc.; ., Dr. Ir. Ketut Srie Marhaeni Julyasih, M.
Jurnal Pendidikan Biologi undiksha Vol 4, No 2 (2017)
Publisher : Undiksha

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Penelitian ini bertujuan untuk (1) mengetahui jumlah koloni jamur endofit yang terdapat pada satu gram sampel akar, batang dan daun tanaman jeruk siam (Citrus nobilis) yang terindikasi sehat dan sakit, (2) mengetahui genus jamur endofit yang terdapat pada sampel akar, batang, dan daun tanaman jeruk siam (Citrus nobilis). Penelitian ini menggunakan pendekatan kualitatif dengan jenis penelitian deskriptif. Subyek dalam penelitian ini adalah jamur endofit dan obyek dalam penelitian ini adalah isolat jamur endofit yang diisolasi dari bagian akar, batang dan daun tanaman jeruk siam (Citrus nobilis) dari perkebunan di Desa Kintamani, Bangli, Bali. Sampel diperoleh dengan menggunakan teknik purposive sampling. Jumlah koloni jamur endofit pada tanaman jeruk siam diperoleh dengan menggunakan teknik pengenceran Standart Plate Count dan identifikasi genus jamur endofit dengan melakukan pengamatan makroskopis dan mikroskopis pada isolat jamur endofit. Hasil penelitian menunjukkan (1) jumlah koloni jamur endofit pada bagian akar sebesar 4,8 x 105 CFU/gram, pada batang 2,9 x 105 CFU/gram dan pada daun sebesar 1,4 x 105 CFU/gram, (2) genus jamur endofit ditemukan sebanyak 11 isolat yang meliputi genus Aspergillus, Penicillium, Mucor dan Fusarium. Kata Kunci : Isolat jamur, Jamur endofit, Jeruk siam (Citrus nobilis). This study aims to (1) determine the number of endophytic fungal colonies needed in one gram sample of roots, stems and leaves of Siam Orange plants (Citrus nobilis) which are indicated to be healthy and infected, (2) find genus of endophytic fungi found in samples of roots, stems , and leaves of Siam Orange plant (Citrus nobilis). This study uses qualitative descriptive research. The subjects in this study were endophytic fungi and the object of this study was fungi isolates of endophytic that was isolated from the roots, stems and leaves of Siam Orange plant (Citrus nobilis) from plantations in the village of Kintamani, Bangli, Bali. Samples were obtained by using purposive sampling technique. The number of endophytic fungal colonies in Siam Orange plants was obtained by using the Standard Plate Count dilution technique and collecting endophytic fungi genus by making macroscopic and microscopic observations on endophytic fungal isolates. The results showed (1) the number of endophytic fungal colonies in the root section was 4.8 x 105 CFU / gram, on the stem 2.9 x 105 CFU / gram and on the leaves was 1.4 x 105 CFU / gram, (2) the genus Endophytic fungi were found as many as 11 isolates containing the genera Aspergillus, Penicillium, Mucor and Fusarium.keyword : Fungi isolates, Endophytic Fungi, Siam Orange (Citrus nobilis).

PENGEMBANGAN UNIT KEGIATAN BELAJAR MANDIRI BERBASIS PENDEKATAN STEM PADA MATERI SISTEM RESPIRASI DENGAN BERBANTUAN EDMODO UNTUK KELAS XI MIPA DI SMA Dwipayanti, Kadek Intan; Citrawathi, Desak Made; Julyasih, Ketut Srie Marhaeni
Jurnal Pendidikan Biologi undiksha Vol 7, No 2 (2020)
Publisher : Undiksha

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Penelitian ini bertujuan untuk mengembangkan bahan ajar berupa Unit Kegiatan Belajar Mandiri (UKBM) berbasis pendekatan STEM (Science, Technology, Engineering and Mathematics) pada materi sistem respirasi dengan bantuan e- learning edmodo sebagai pengiriman bahan ajar dan menguji kelayakan, kepraktisan dan keefektifan dari UKBM yang dikembangkan. Penelitian ini adalah penelitian pengembangan dengan menggunakan model ADDIE (analyze, design, development, implementation, dan evaluation). Subjek uji coba produk dalam penelitian ini adalah siswa kelas XI MIPA 3. Instrumen yang digunakan dalam penelitian adalah angket dan soal materi sistem respirasi. Data penelitian dianalisis dengan teknik analisis statistik deskriptif kualitatif dan analisis statistik deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa: (1) kelayakan UKBM berbasis pendekatan STEM dari ahli media mendapatkan presentase skor sebesar 90,6% dengan kriteria sangat layak dan dari ahli materi mendapatkan presentase skor sebesar 80,9% dengan kriteria layak, (2) kepraktisan UKBM berbasis pendekatan STEM mendapatkan presentase sebesar 89,8% dengan kriteria sangat praktis, (3) keefektifan UKBM berbasis pendekatan STEM mendapatkan presentase sebesar 77,78% dengan kriteria baik. Berdasarkan hasil penelitian tersebut, UKBM berbasis pendekatan STEM materi sistem respirasi dapat digunakan sebagai alternatif baru dalam bahan ajar di sekolah.

DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD) DISEASE BY POLYMERASE CHAIN REACTION (PCR) AND PROTEIN ANALYSIS USING SDS PAGE (A Review) I Gede Putu Wirawan; Ketut Srie Marhaeni Julyasih
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Citrus Vein Phloem degeneration (CVPD) is the most important disease and a major cause of yield loss citrus plantations in almost all countries, especially Asia and Africa. CVPD disease is caused by gram negative bacteria, Liberobacter asiaticum L. Pathogens can not be cultured in vitro, but can be detected by PCR using 16S rDNA fragment as a primer and by electron microscopy. The use of PCRusing specific primer pair can detect infected plants more accurately. Citrus plant that infected by CVPD disease contain a very specific protein that produced by the pathogen. The specific protein molecules can be detected by comparing the protein bands of infected citrus plants and the healthy one which have molecular weight of approximately 16 kDa and 66 kDa, whereas in healthy citrus plant that protein molecule was not found.

POTENTIAL EFFECT OF MACRO ALGA Caulerpa sp. AND Gracilaria sp. EXTRACT LOWERING MALONDIALDEHYDE LEVEL OF WISTAR RATS FED HIGH CHOLESTEROL DIET Ketut Srie Marhaeni Julyasih; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Seaweed has potential nutrient content such as carotenoids, vitamins, fatty acids, carbohydrates, minerals, and other essential substances. Carotenoids have important biological functions as an antioxidant, and immunostimulatory which can prevent the disease, anti-inflammatory, anti-stress, anti-aging, and protect the skin from the harmful effects of ultraviolet radiation. Seaweed generally consumed as a vegetable by people in Bali, known as the local name Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.).. So far there has been no report or results of research on the effects of extract ethanol of Bulung Boni (Caulerpa sp.) and Bulung Sangu (Gracilaria sp.) as an antioxidant that can prevent lipid peroxidation which can be seen in decreased level of MDA in liver tissue or blood plasma. Therefore it is necessary to determine of plasmaMDA level of Wistar rat after fed high cholesterol diet treated with extract ethanol of Caulerpa sp. and Gracillaria sp. This experimental study used completely randomized design. Research using total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol especially in negative control. The study consisted of negative control group (standard diet), positive control group (high cholesterol diet), high-cholesterol diet with Caulerpa sp. extract dose of 20 mg and 60 mg/100 g, high cholesterol diet with Gracilaria sp. extract dose of 20 mg and 60 mg/100 g body weight rat per day.The study resulted that rats fed high cholesterol diet with treated extract ethanol Caulerpa sp. and Gracilaria sp. with a dose of 20 mg and 60 mg per 100 g body weight rat / day had plasma MDA level significantly lower (p <0.05) compared with rats fed high cholesterol diet without treated with extract of Caulerpa sp. and Gracilaria sp.

INCREASING LOCAL FRUITS COMPETITIVENESS IN ENTERING THE TOURISM MARKET IN BALI I Gede Putu Wirawan; Ketut Srie Marhaeni Julyasih; Wayan Adiartayasa; I Nyoman Wijaya; I Putu Anom
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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The study focused on improving the fruit quality supported by handling of the cultivation, harvest and post-??harve st handling such as fruit packing, transportation, storage, and others, as well as marketing strategiesand regulations that are ne eded locally, so local Bali fruits can compete better in the Bali tourism market.The purposes of this study were to find some problems face by the local fruits to e nter the tourism marke t inBali. The local fruits studied were citrus, salacca, mangoes, mangosteen, banana, and papaya. This study wasthrough multiple surveys such as, inte rviews with farme rs, whole salers, fruit suppliers, and the manage rsof the hote ls and restaurants. The results of this study found some local fruits have e nte red the tourismmarket in Bali, in addition, local people in Bali te nd to consume the imported fruits for some culturalceremony, events and others.The results of this study showthat the main problems in the local fruits suchas citrus and banana were harvesting, packaging, storage, and transportation. The laboratory studies foundthat some microbes and inse cts infe cted local fruits due to the lackof handling such as packaging, storage,and transportation. The findings of this study was local fruits such as orange s and bananas face manyobstacles ranging from harvest, post-??harvest management, transportation, and the marketing strategies.Many problem has found on bananas such as damaged on storage, as well as citrus fruits. Improvements tothe appearance of the fruit has been tried to do by the application of emulsion of he rbal oils, oleic acid, wax,twee n, and the results showe d the fruits still in a fre sh condition afte r four weeks of treatme nt while theuntre ated fruits were rotten due tothe infe ction of microbes. The blackspots of insects ormicrobes infectionson the treated fruits can be eliminated as well. Some inse cts and microbial attacks were found that leads tofruit rot which can essentially be removed by tre atment with antimicrobial tre atment. This research tries tochart the effects on the competitiveness of local fruits, such as continuity of production, standardization andregulation which could be developed at the local level in the village community.

Potensi Alga Merah dan Alga Hijau untuk Menghambat Pertumbuhan Bakteri Eschericia coli KETUT SRIE MARHAENI JULYASIH; NI PUTU RISTIATI; IDA BAGUS PUTU ARNYANA
Agrotrop : Journal on Agriculture Science Vol 10 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

Potential of Red Algae and Green Algae to Inhibit Escherichia coli Bacteria Growth. The use of synthetic chemicals as a control for the growth of E. coli bacteria that causes of diarrhea children can cause adverse effects on health, so it needs natural control ingredients that do not cause impacts on human health. One natural controller to inhibit bacterial growth is by utilizing natural sources, including seaweed. The purpose of this study was to examine the potential of seaweed as an anti-bacterial agent of E. coli. The research used seaweed extract of Eucheuma spinosum, Euchuema cottonii, Gracilaria spp., and Caulerpa spp. The test parameters observed were the inhibition zone (mm) of each seaweed extract treatment using a ruler and measured the inhibition zone distance. The results showed that E.cottonii seaweed extract had the highest inhibitory ability with an inhibition zone diameter of 11.1 mm, including the moderately sensitive category, then Caulerpa spp. with a inhibition zone diameter of 10.0 mm, Gracilaria spp has the ability to inhibit E.coli bacteria with a inhibition zone diameter of 6.9 mm, including the insensitive category, and the lowest in seaweed E. spinosum with a inhibition zone diameter of 4.2 mm. including the insensitive category.

Potensi Rumput Laut Dalam Menghambat Pertumbuhan Jamur Aspergillus flavus KETUT SRIE MARHAENI JULYASIH; ARIKA PURNAWATI
Agrotrop : Journal on Agriculture Science Vol 9 No 1 (2019)
Publisher : Fakultas Pertanian Universitas Udayana

Potential of Seaweed in the Inhibition of Fungi Growth Aspergillus flavus. This use of synthetic chemicals as a controller of fungal growth in foodstuffs can have adverse effects on health, so it needs natural control ingredients that do not have an impact on human health. Various types of fungi such as Aspergillus flavus attack a lot of food items after harvest, these fungi can produce aflatoxin which is very toxic to consumers. Aflatoxin cannot be neutralized through cooking so efforts to avoid fungal contamination need to be done. One of the natural fungi controllers is by utilizing natural resources, including seaweed. Development of seaweed as an antifungal is an alternative that needs to be realized. For this reason, it is necessary to examine the potential inhibitory of seaweed A. flavus development. This study examined six types of seaweed like Gracilaria verrucosa, Euchema spinosum, Ulva lactuca, Ulva reticulata, Euchema edule, and Padina sp. The results showed that Padina sp. seaweed has a very strong potential inhibitory compared to other types of seaweed.

Co-Authors ., Monika Megawati Ferdiana Dara ., NI MADE DWI RIANI SARASWATI Andrianus Sembiring Arika Purnawati Bima Apry Anggara Desak Made Citrawathi Dwipayanti, Kadek Intan I GEDE PUTU WIRAWAN I Made Oka Riawan I NYOMAN WIJAYA I Putu Anom I Putu Parwata I Wayan Sukra Warpala Ida Ayu Putu Suryanti Ida Bagus Putu Arnyana Immanuel Pratama Kadek Intan Dwipayanti Luh Putu Candra Apriliani Monika Megawati Ferdiana Dara . Ni Komang Rossa Sri Savitri Ni Luh Astria Yusmalinda NI MADE DWI RIANI SARASWATI . Ni Putu Dian Pertiwi Ni Putu Dian Pertiwi, Ni Putu Dian Ni Putu Nila Ristiari Ni Putu Ristiati Ni Putu Sri Ratna Dewi Ni Putu Ulan Rosita Putri NP Sri Ratna Dewi Parista, Ni Made Sudiyasih Ratna Dewi, NP Sri Ristiari, Ni Putu Nila Rita Rachmawati Rosita Putri, Ni Putu Ulan WAYAN ADIARTAYASA

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