Article Per Year (5 Year)
p-Index From 2020 - 2025
0.947
P-Index
This Author published in this journals
All Journal Journal of Tropical Biodiversity and Biotechnology The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Community Journal Jurnal Farmasetis
Febri Wulandari
Universitas Muhammadiyah Surakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Curcumin Analogs, PGV-1 and CCA-1.1 Exhibit Anti-migratory Effects and Suppress MMP9 Expression on WiDr Cells Febri Wulandari; Muthi' Ikawati; Mitsunori Kirihata; Jun-Ya Kato; Edy Meiyanto
The Indonesian Biomedical Journal Vol 13, No 3 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i3.1583

Abstract

BACKGROUND: Colon cancer is still a crucial concern in the development of chemotherapeutic drugs due to the drug resistance phenomenon and various side effects to patients. One of the newest compound that show anticancer activities against several cancer cells, Chemoprevention Curcumin Analog 1 (CCA-1.1), has increasingly been explored to overcome the limitation of conventional drugs.METHODS: We evaluated the anti-migratory effect of CCA-1.1 and Pentagamavunone-1 (PGV-1) by using WiDr colon cancer cells. The expression profiles of Tumor Protein 53 (TP53) and Matrix Metalloproteinase-9 (MMP9) in colon cancer were obtained from the UALCAN database. Survival outcomes of TP53 and MMP9 in colon cancer patients were analyzed using the Kaplan-Meier method. We used 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT), scratch wound healing, and gelatin zymography assays to observe the cytotoxic effect, anti-migratory activity, and MMP9 expression, respectively, in CCA-1.1 or PGV-1-treated cells.RESULTS: Level of MMP9 was found significantly overexpressed in the primary tumor and metastasis nodal, while TP53 mutation sample types were observed and influenced the survival outcome in colon cancer patients. CCA-1.1 and PGV-1 exhibited strong cytotoxic activity after 24 and 48 h treatment against WiDr cells. The migration assay demonstrated that PGV-1 and CCA-1.1 at 1 mM inhibited cell migration up to 40% after 48 h in single and combination with doxorubicin. The MMP9 expression was significantly inhibited by 0.5 mM CCA-1.1.CONCLUSION: This study emphasizes that the anti-migratory effect of CCA-1.1 is better than PGV-1 via MMP9 suppression on WiDr. Thus, CCA-1.1 is prominent to be developed as an anti-metastatic agent.KEYWORDS: chemopreventive curcumin analog 1.1 (CCA-1.1), PGV-1, WiDr cells, anti-migration, MMP9
Edukasi dan Pemeriksaan Terkait Risiko Penyakit Diabetes dan Asam Urat di Dusun Tinggen, Minggir, Sleman Febri Wulandari; Muhammad Maslakul Abid; Fiony Diva Rachmawati; Hardian Wahyu Widianto
I-Com: Indonesian Community Journal Vol 3 No 3 (2023): I-Com: Indonesian Community Journal (September 2023)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33379/icom.v3i3.3189

Abstract

Tingginya prevalensi penyakit populer, diantaranya diabetes dan asam urat, menjadi kewaspadaan awal untuk berusaha meminimalisir faktor risikonya. Di Sleman, kedua penyakit ini selalu menduduki peringkat lima besar di Puskesmas daerah setempat. Kegiatan edukasi dan pemeriksaan kesehatan dapat menjadi wadah untuk meningkatkan pengetahuan masyarakat terkait risiko penyakit populer, serta menekankan pentingnya pola hidup sehat sebagai tindakan pencegahan. Kegiatan ini dilakukan dengan penyuluhan dan pemeriksaan kadar gula darah dan asam urat, dilanjutkan konseling secara personal. Dilakukan pretest dan posttest sederhana untuk menilai efektivitas kegiatan edukasi. Hasil menunjukkan bahwa kegiatan edukasi terhadap 35 warga usia produktif mampu meningkatkan pemahaman masyarakat terkait penyakit populer, didukung dengan pemeriksaan kesehatan dan konseling personal yang dilakukan. Pemberdayaan masyarakat untuk menjalani pola hidup sehat dapat menjadi langkah untuk peningkatan kesehatan individu dan lingkungan.
The Synergistic Effect of Combination of Pentagamavunone-1 with Diosmin, Galangin, and Piperine in WiDr Colon Cancer Cells: In vitro and Target Protein Prediction Muthi Ikawati; Hajidah Musyayyadah; Yurananda Magnalia Putri; Ummi Maryam Zulfin; Febri Wulandari; Dyaningtyas Dewi Pamungkas Putri; Edy Meiyanto
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.80975

Abstract

Pentagamavunone-1 (PGV-1) is a curcumin analog with a prominent anti-cancer potency in vitro and in vivo for several cancer types, including colon cancer. Combining PGV-1 with natural compounds such as diosmin, galangin, and piperine can enhance its effectiveness due to their promising chemoprevention properties. We aimed to evaluate the effectiveness of combining PGV-1 with diosmin, galangin, or piperine for colon cancer by using in vitro and bioinformatic approaches to predict their target proteins. WiDr cells were used as a model for colon adenocarcinoma (COAD). The cell viability under a single or combination treatment of PGV-1 and diosmin, galangin, or piperine was evaluated using direct counting by the trypan blue exclusion test. SwissTargetProtein, UALCAN, and OncoLnc were utilized to predict target proteins of the compounds in COAD, the expression level of target proteins in COAD, and the survival rate of patients with overexpressed target proteins, respectively. The IC50 values for PGV-1, diosmin, galangin, and piperine were 2.8´10-2 µg/mL, 81 µg/mL, 7 µg/mL, and 172 µg/mL, respectively. All the tested natural compounds showed synergistic effects when combined with PGV-1 at low concentrations. Eleven proteins that were overexpressed in COAD were identified as potential targets. Overlapped predicted targets of PGV-1 and galangin or piperine were CDK1, MET, and TOP2A. The high expression of another set of predicted target proteins, SCD, CA9, and SQLE, led to lower survival rates in COAD patients. We concluded that combinations of PGV-1 with natural compounds can synergistically enhane its anti-cancer activity for colon cancer.
Integrative Bioinformatics Reveals the Lactate Dehydrogenase B (LDHB) Significance in Colon Adenocarcinoma Wulandari, Febri; Hanifa, Mila
Molecular and Cellular Biomedical Sciences Vol 7, No 2 (2023)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v7i2.346

Abstract

Background: Lactate dehydrogenase B (LDHB), a typical oxidoreductase for converting lactate to pyruvate in the glycolysis process, takes a complex function in the progression of cancer cells. Even so, the profile of LDHB relevance in colon adenocarcinoma (COAD) remains ambiguous. Hence this study analyzed the expression and co-expression profile of LDHB, and its immune correlation in COAD.Materials and method: The mRNA expression and co-expression of LDHB in COAD were retrieved from UALCAN. The immune infiltration levels of LDHB from B cells, CD4+ T cells, CD8+ T cells, macrophages, neutrophils, and dendritic cells in COAD were assessed using the TIMER database. For assessing gene ontology and the KEGG pathway, DAVID v6.8 was utilized. The protein-protein interaction of LDHB-correlated genes was analyzed using STRINGDB and Cytoscape.Results: Significantly high expression of LDHB in COAD was spotted in several sample types and associated with a poor overall survival rate. Further, LDHB corresponded to the level of CD4+, macrophages, and myeloid-derived suppressor cell (MDSC) immune infiltrating cells. The co-expression of LDHB was associated with several essential genes for cell cycle progression.Conclusion: The findings of this study indicate an upcoming involvement of LDHB in COAD tumorigenesis and prognosis. Additionally, this study highlights the immune correlation of LDHB in COAD as preliminary data in developing diagnosis and treatment with a novel immune checkpoint in COAD.Keywords: lactate dehydrogenase, colon adenocarcinoma, expression, survival, immune
Studi Docking Molekuler Senyawa [(5-prop-2-enylpyrimidin-2-yl) propanoate] dan [4-((1e)-buta-1,3-dienyl)phenol] Terhadap Protein Er-Α, Er-Β, dan IKK sebagai Agen Sitotoksik Hindami, Fathan Taqwim; Da’i, Muhammad; Fauzi, Ahmad; Wulandari, Febri
Jurnal Farmasetis Vol 13 No 3 (2024): Jurnal Farmasetis: Agustus 2024
Publisher : LPPM Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32583/far.v13i3.2316

Abstract

Kanker merupakan salah satu penyebab kematian yang tergolong penyakit tidak menular dan kasusnya terus bertambah. Kanker payudara sering ditemukan pada wanita di seluruh dunia. 1’-Acetoxychavicol acetate (ACA) adalah fenilpropanoid dalam rimpang spesies jahe Alpinia. ACA menunjukkan aktivitas sitotoksik pada berbagai lini sel tumor, salah satunya MCF7 (sel kanker payudara) dengan nilai IC50 berkisar antara 5 - 50 μM tanpa toksisitas terhadap sel normal. Tujuan dilakukan penelitian ini adalah mengetahui senyawa turunan ACA memiliki aktivitas antikanker terhadap protein kanker payudara secara in silico dengan docking molekuler. Protein target yang digunakan adalah ER-α, ER-β, dan IKK. Senyawa uji yang digunakan adalah senyawa 1 [(5-prop-2-enylpyrimidin-2-yl) propanoate], senyawa 2 [4-((1e)-buta-1,3-dienyl)phenol], lalu terdapat tambahan doksorubisin (sebagai kontrol positif), dan metformin (sebagai kontrol negatif). Parameter docking berupa nilai afinitas, semakin kuat ikatan ligan terhadap reseptor, maka semakin baik prediksi docking molekuler dinyatakan dengan nilai afinitas ikatan yang semakin kecil. Parameter lain yaitu nilai Root Mean Square Deviation (RMSD). Pengujian dinyatakan valid ketika hasil RMSD ≤ 2 Å. Dilakukan visualisasi juga yang menghasilkan interaksi antara residu asam amino dengan ligan. Hasil nilai afinitas senyawa 1 dengan protein ER-α, ER-β, dan IKK berturut-turut -6.3 kkal/mol, -6.2 kkal/mol, dan -5.8 kkal/mol. Pada senyawa 2 berturut-turut -5.7 kkal/mol, -6.2 kkal/mol, dan -6.8 kkal/mol. Hasil RMSD senyawa 1 berturut-turut 0.587 Å, 1.15 Å, 1.047 Å dan senyawa 2 berturut-turut 0.177 Å, 1.146 Å, 1.295 Å. Hasil visualisasi pada senyawa 1 dan senyawa 2 menunjukkan kemiripan dengan doksorubisin (kontrol positif). Hasil tersebut menandakan senyawa 1 [(5-prop-2-enylpyrimidin-2-yl) propanoate] dan senyawa 2 [4-((1e)-buta-1,3-dienyl)phenol] berpotensi sebagai agen antikanker payudara.
Co-Authors Dyaningtyas Dewi Pamungkas Putri Edy Meiyanto Edy Meiyanto Fauzi Ahmad Muda Fiony Diva Rachmawati Hajidah Musyayyadah Hanifa, Mila Hardian Wahyu Widianto Hindami, Fathan Taqwim Jun-Ya Kato Mitsunori Kirihata Muhammad Da'i Muhammad Maslakul Abid Muthi Ikawati Muthi Ikawati Ummi Maryam Zulfin Yurananda Magnalia Putri