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All Journal AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA CROP AGRO, Scientific Journal of Agronomy
I.G.B. Adwita Arsa
Nusa Cendana University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Journal : BERITA BIOLOGI

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ANALISIS FENETIK JAGUNG RAS LOKAL NUSA TENGGARA TIMUR UMUR GENJAH BERDASARKAN KARAKTER AGRONOMI DAN INTER SHORT SEQUENCE REPEATS [Phenetic analysis of Local Landraces of Early Maturity Maize from East Nusa Tenggara based on Agronomic Traits and Inter Short Sequence Repeats] Yulita, Kusumadewi Sri; Bora, Charles Y.; Arsa, IGB Adwita; Murniningsih, Tri
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.36 KB) | DOI: 10.14203/beritabiologi.v14i3.1835

Abstract

Maize having maturity age less than 80 HST (days after planting) has played an important roles as part of food security system for the people of NTT since they can be harvested earlier than common maize and usually consumed by children. Local landraces of maize are usually heterogenous homozygote due to traditional farming system where the farmers used their own harvested maize for generations resulted in low level of heterogeinity. This study was aimed to assess phenotypic and genetic variability among 12 accessions of local maize on the basis of their agronomic perfomance and Inter Short Sequence Repeats profiles. Two accessions of non- early maturity maize were included as control. Ten agronomic traits and eight ISSRs ‘s primers were used to perform a phenetic analysis using unweighted pair group method with aritmethical average(UPGMA).Clustering dendrogram based on agronomic characters suggested that all accessions were forming three groups on the basis of their maturity (very early mature, early mature, and intermediate mature).On the other hand, analysis using ISSR profiles resulted on random grouping across the accesions.All accessions of early mature age having taxonomic distance of 0.83 and genetic similarity of 57%. This indicated that even though the landraces were open pollinated which allowed cross pollinations during the flowering time, phenotipic and genotipic variations observed to be considerably low.
RESPON TANAMAN JAGUNG VARIETAS LOKAL NTT UMUR SANGAT GENJAH (PENA TUNU’ ANA’) TERHADAP CEKAMAN KEKERINGAN  Murningsih, Tri; Yulita, Kusumadewi Sri; Bora, Charles Y.; Arsa, I.G.B. Adwita
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1865

Abstract

In dry land areas such as Nusa Tenggara Timur (NTT), low production of maize may due to selection of using local varieties that are mostly drought tolerant.One of the local varieties known as Pena Tunu? Ana? have been harversted in very early age of 1.5 month, hence it was categorised as very early mature maize. This variety is therefore play an important role in the food security system for people in NTT as they can be harvested earlier than other varieties. However, no information has yet on how this variety response to drought stress. Drought stress is one of the factors causing the decline in maize production. This study was conducted at experimental house in Cibinong Science Centre (CSC) to evaluate responses of Pena Tunu? Ana? against drought stress treatment. The experiment was done in six degree of drought treatments, 0 (control), 4, 8, 12, 16 and 20 days with 4 replications. Parameters measured were soil water, relative leaf water,proline and sugar content in leaves and roots at each level of drought stressed. Results showed that with increasing level of drought stress,soil water and relative leaf water content were decreased while proline and sugar content were increased. At the same level of drought stress, accumulation of proline and sugar in the roots was higher than those in the leaves. Drought stress terminated at 16 days, where the roots content of proline reached 10.36 mg/g and sugar 110.91 mg/g, while the leaves content of proline was of 2.84 mg/g and sugar was of 38.44 mg/g. At the level of drought stress during 16 days, the plants suffered temporary wilting but refreshed after watering.
RESPON TANAMAN JAGUNG VARIETAS LOKAL NTT UMUR SANGAT GENJAH (PENA TUNU’ ANA’) TERHADAP CEKAMAN KEKERINGAN  Tri Murningsih; Kusumadewi Sri Yulita; Charles Y. Bora; I.G.B. Adwita Arsa
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1865

Abstract

In dry land areas such as Nusa Tenggara Timur (NTT), low production of maize may due to selection of using local varieties that are mostly drought tolerant.One of the local varieties known as Pena Tunu’ Ana’ have been harversted in very early age of 1.5 month, hence it was categorised as very early mature maize. This variety is therefore play an important role in the food security system for people in NTT as they can be harvested earlier than other varieties. However, no information has yet on how this variety response to drought stress. Drought stress is one of the factors causing the decline in maize production. This study was conducted at experimental house in Cibinong Science Centre (CSC) to evaluate responses of Pena Tunu’ Ana’ against drought stress treatment. The experiment was done in six degree of drought treatments, 0 (control), 4, 8, 12, 16 and 20 days with 4 replications. Parameters measured were soil water, relative leaf water,proline and sugar content in leaves and roots at each level of drought stressed. Results showed that with increasing level of drought stress,soil water and relative leaf water content were decreased while proline and sugar content were increased. At the same level of drought stress, accumulation of proline and sugar in the roots was higher than those in the leaves. Drought stress terminated at 16 days, where the roots content of proline reached 10.36 mg/g and sugar 110.91 mg/g, while the leaves content of proline was of 2.84 mg/g and sugar was of 38.44 mg/g. At the level of drought stress during 16 days, the plants suffered temporary wilting but refreshed after watering.
ANALISIS FENETIK JAGUNG RAS LOKAL NUSA TENGGARA TIMUR UMUR GENJAH BERDASARKAN KARAKTER AGRONOMI DAN INTER SHORT SEQUENCE REPEATS Kusumadewi Sri Yulita; Charles Y. Bora; IGB Adwita Arsa; Tri Murniningsih
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i3.1835

Abstract

Maize having maturity age less than 80 HST (days after planting) has played an important roles as part of food security system for the people of NTT since they can be harvested earlier than common maize and usually consumed by children. Local landraces of maize are usually heterogenous homozygote due to traditional farming system where the farmers used their own harvested maize for generations resulted in low level of heterogeinity. This study was aimed to assess phenotypic and genetic variability among 12 accessions of local maize on the basis of their agronomic perfomance and Inter Short Sequence Repeats profiles. Two accessions of non- early maturity maize were included as control. Ten agronomic traits and eight ISSRs ‘s primers were used to perform a phenetic analysis using unweighted pair group method with aritmethical average(UPGMA).Clustering dendrogram based on agronomic characters suggested that all accessions were forming three groups on the basis of their maturity (very early mature, early mature, and intermediate mature).On the other hand, analysis using ISSR profiles resulted on random grouping across the accesions.All accessions of early mature age having taxonomic distance of 0.83 and genetic similarity of 57%. This indicated that even though the landraces were open pollinated which allowed cross pollinations during the flowering time, phenotipic and genotipic variations observed to be considerably low.
Co-Authors Antonius S. S. Ndiwa Antonius SS Ndiwa Ariffin Ariffin Bora, Charles Y. Bora, Charles Y. Charles Y. Bora Charles Y. Bora Herianus J.D. Lalel Kusumadewi Sri Yulita KUSUMADEWI SRI YULITA Kusumadewi Sri Yulita Nurul Aini Shirly S. Oematan Tri Murningsih Tri Murningsih, Tri Tri Murniningsih Tri Murniningsih Yosep Seran Mau