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. Mujosemedi
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Uji Sitotoksik Kuantitatif Ekstrak Biji Srikaya (Annona squamosa L.) pada Kultur Sel Limfosit T Pasien SLE In Vitro Pratama, Anindhito Kurnia; Balgis, .; Mujosemedi, .
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Systemic Lupus Erythematosus is an autoimmune disease, mediated by lymphocytes. Methanol extract of Annona squamosa L. seed contains acetogenin, which can inhibit formation of ATP in the abnormal cell that acts as a source of energy that has selective properties of the abnormal cells only. The purpose of this study was to know the cytotoxic effects of Annona squamosa L. seed extract on T lymphocyte cell cultures of SLE patient in vitro. Methods: This study was an experimental laboratory research with post-test control group design. The sample was T lymphocyte cultures of SLE patients in the active phase. Cytotoxic effects of Annona squamosa L. seed extract on T lymphocyte cell culture of SLE patient in vitro were measured with the MTT assay then analyzed with regression analysis to determine the inhibitory concentration of fifty (IC50). Results: The IC50 of methanol extract of Annona squamosa L. seed on lymphocyte cell cultures of SLE patients was 0.17 µg/mL, active criteria and normal sample was 0.06 µg/mL., active criteria. While IC50 of cyclophosphamide for treatment of SLE samples was 16.80 µg/mL and for normal sample was 39.24 µg/mL. Conclusions: Based on this research, methanol extract of Annona squamosa L. seed had cytotoxic effects on  lymphocyte cell cultures of SLE patient in vitro with active IC50 criteria Keywords: SLE, Annona squamosa L. seed, cytotoxic, T lymphocyte, IC50
Uji Sitotoksik Ekstrak Biji Pare (Momordica charantia L.) pada Kultur Sel Limfosit T Pasien LES In Vitro Permana, Galih Indra; Balgis, .; Mujosemedi, .
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Systemic Lupus Erythematosus (SLE) is an autoimmune disease, mediated by lymphocytes. Wrong regulation in immune system causes autoreactivity of T lymphocytes and hyperactivity of B lymphocytes then causes inflammation in site organ. Extract of bitter melon seeds contains ribosome inactivating proteins (RIPs), momordin I, and momordin II which can induce apoptosis in the cells and have cytotoxic effects. This study is to examine the cytotoxic effects of bitter melon seeds extract on cultured T lymphocytes of SLE patient in vitro. Methods: This study was an experimental laboratory research with posttest control group design. Sample was culture of T lymphocytes from SLE patient with the active phase. T lymphocytes culture was divided in four groups that were bitter melon seeds extract, cyclophosphamide, control media, and control cells. Cytotoxic effects were measured with MTT assay then the data were analyzed with statistical regression analysis to determine the IC50. Results: IC50 of bitter melon seeds extract on culture of T lymphocytes cell of SLE patient was 1704.07 µg/ml and normal sample was 19.20 µg/ml. While IC50 of cyclophosphamide for treatment of SLE sample was 16.80 µg/ml and for normal sample was 39.24 µg/ml. Extract of bitter melon seeds had weak cytotoxic effect on T lymphocyte cell culture of SLE patient and moderately active cytotoxic effect on T lymphocyte cell culture of normal sample. Conclusion: Extract of bitter melon seeds had weak cytotoxic effects on T lymphocyte cell culture of SLE patient with IC50 was 1704.07 µg/ml. Keywords: SLE, bitter melon seed extract, cytotoxic, lymphocytes T
The Influence of Andropause on Incidence of Depression in Males at Subdistrict Jebres, Surakarta Iriyanti Maya Sari Barutu; . Mujosemedi; Andri Iryawan
Nexus Kedokteran Komunitas Vol 4, No 1 (2015): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: One of the symptoms in males with andropause is mood depression. Andropauses occurrence in a man may increase the incidence of depression. This research aimed to know the influence of andropause on incidence of depression in males at Subdistrict Jebres, Surakarta. Method: This observational analytic which used cross sectional method was held in June-July 2014. The subjects were males 45 years old or older who lived at Subdistrict Jebres, Surakarta. Sixty males who were chosen by purposive random sampling method as the subjects consisted of 30 males with andropause and 30 males without andropause. Data collected through questionarie were analyzed with Chi-Square and Odds Ratio. Result: The analysis showed that 20 out of 30 males with andropause had depression. But, only 7 out of 30 males without andropause had depression. The data analysis with ? = 0.05 showed p = 0.001 (p < 0.05); Odds Ratio = 6.571. There was a significant difference of depressions occurrence between males with andropause and males without andropause at Subdistrict Jebres, Surakarta. Conclusion: There is influence of andropause on incidence of depression in males at Subdistrict Jebres, Surakarta which the number of depression prevalence is bigger at andropauses group. Keywords: Andropause, depression, males
Uji Sitotoksik Kuantitatif Ekstrak Biji Srikaya (Annona squamosa L.) pada Kultur Sel Limfosit T Pasien SLE In Vitro Anindhito Kurnia Pratama; . Balgis; . Mujosemedi
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Systemic Lupus Erythematosus is an autoimmune disease, mediated by lymphocytes. Methanol extract of Annona squamosa L. seed contains acetogenin, which can inhibit formation of ATP in the abnormal cell that acts as a source of energy that has selective properties of the abnormal cells only. The purpose of this study was to know the cytotoxic effects of Annona squamosa L. seed extract on T lymphocyte cell cultures of SLE patient in vitro. Methods: This study was an experimental laboratory research with post-test control group design. The sample was T lymphocyte cultures of SLE patients in the active phase. Cytotoxic effects of Annona squamosa L. seed extract on T lymphocyte cell culture of SLE patient in vitro were measured with the MTT assay then analyzed with regression analysis to determine the inhibitory concentration of fifty (IC50). Results: The IC50 of methanol extract of Annona squamosa L. seed on lymphocyte cell cultures of SLE patients was 0.17 g/mL, active criteria and normal sample was 0.06 g/mL., active criteria. While IC50 of cyclophosphamide for treatment of SLE samples was 16.80 g/mL and for normal sample was 39.24 g/mL. Conclusions: Based on this research, methanol extract of Annona squamosa L. seed had cytotoxic effects on lymphocyte cell cultures of SLE patient in vitro with active IC50 criteria Keywords: SLE, Annona squamosa L. seed, cytotoxic, T lymphocyte, IC50
Cytotoxic Assay of Soursop Leaf Ethanol Extract (Annona muricata Linn.) on Breast Cancer Cell Line (T47D) In Vitro Dwiana Ardianti; . Suyatmi; . Mujosemedi
Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Cancer is a genetical disorder which involves dynamic changes of genome, that it causes abnormal cell growth, invasion and metastasis of cell. Breast cancer is found mostly on women. There are many phytochemistry bioactive compounds found in soursop plant. One of the most important component is annonaceous acetogenins. This study aimed to determine the cytotoxic effect of soursop leaf ethanol extract on breast cancer cell line (T47D). Methods: This was experimental laboratory research. The cytotoxic effects of Soursop Leaf Ethanol Extract studied on cell line of breast cancer (T47D) in vitro were measured by MTT assay. The cytotoxic effect of the extract was compared to doxorubicyn. The data were analyzed to determine the inhibitory concentration of 50% (IC50). Results: IC50 of soursop leaf ethanol extract on breast cancer cell line (T47D) was 69.250mg/ml and doxorubicyn was < 0.195mg/ml. Conclusion: Based on the study, soursop leaf ethanol extract has a weak cytotoxic effect on breast cancer cell line (T47D) with IC50 = 69.25 mg/ml. Keywords: MTT assay, Soursop Leaf Ethanol Extract, Cytotoxicity, Breast cancer cell line (T47D).
Uji Sitotoksik Ekstrak Biji Pare (Momordica charantia L.) pada Kultur Sel Limfosit T Pasien LES In Vitro Galih Indra Permana; . Balgis; . Mujosemedi
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Systemic Lupus Erythematosus (SLE) is an autoimmune disease, mediated by lymphocytes. Wrong regulation in immune system causes autoreactivity of T lymphocytes and hyperactivity of B lymphocytes then causes inflammation in site organ. Extract of bitter melon seeds contains ribosome inactivating proteins (RIPs), momordin I, and momordin II which can induce apoptosis in the cells and have cytotoxic effects. This study is to examine the cytotoxic effects of bitter melon seeds extract on cultured T lymphocytes of SLE patient in vitro. Methods: This study was an experimental laboratory research with posttest control group design. Sample was culture of T lymphocytes from SLE patient with the active phase. T lymphocytes culture was divided in four groups that were bitter melon seeds extract, cyclophosphamide, control media, and control cells. Cytotoxic effects were measured with MTT assay then the data were analyzed with statistical regression analysis to determine the IC50. Results: IC50 of bitter melon seeds extract on culture of T lymphocytes cell of SLE patient was 1704.07 g/ml and normal sample was 19.20 g/ml. While IC50 of cyclophosphamide for treatment of SLE sample was 16.80 g/ml and for normal sample was 39.24 g/ml. Extract of bitter melon seeds had weak cytotoxic effect on T lymphocyte cell culture of SLE patient and moderately active cytotoxic effect on T lymphocyte cell culture of normal sample. Conclusion: Extract of bitter melon seeds had weak cytotoxic effects on T lymphocyte cell culture of SLE patient with IC50 was 1704.07 g/ml. Keywords: SLE, bitter melon seed extract, cytotoxic, lymphocytes T
Co-Authors . Balgis . Suyatmi Andri Iryawan Anindhito Kurnia Pratama Balgis . Dwiana Ardianti Galih Indra Permana Iriyanti Maya Sari Barutu