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All Journal Jurnal Kedokteran Brawijaya Jurnal Ilmiah Kedokteran Wijaya Kusuma YARSI Medical Journal
Ketut Muliartha
Laboratorium Sentral Biomedik, Fakuktas Kedokteran Brawijaya
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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REACTION BIOMARKER MONOCLONAL FRAGMENTATION OF COLLAGEN TYPE IV WITH BLOOD SERUM ACUTE MYOCARDIAL INFARCTION PATIENT RELATED INFECTION Muliartha, Ketut
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 1, No 1 (2012): Edisi Juli 2012
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

Penelitian ini bertujuan untuk mendeteksi penyakit jantung akut myocardial infark dengan menggunakan biomarker antibodi monoclonal fragmentasi kolagen tipe IV. Rupture plak aterosklerosis adalah terjadi karena degradasi kolagen tipe IV. Perviromonas gingivalis adalah salah satu faktor resiko yang terpenting untuk terjadinya akut myocard infark (AMI). Rancangan penelitian ini adalah ekperimental invitro dengan deksriptif cross sectional. Sampel penelitian adalah empat orang sehat (n=4) serta 12 penderita AMI (n=12). Untuk melihat ekpresi enzim MMP-9 dan fragmentasi kolagen tipe IV, kami menggunakan teknik western bloting. Untuk mengisolasi sel limfosit, kami mengimunisasi mencit dengan antigen fragmentasi kolagen tipe IV disertai dengan adjuvant. Selanjutnya dilakukan fusi antara sel limfosit dengan sel myeloma sehingga terbentuk hibridoma dan diuji dengan western bloting serta ELISA teknik. Kemudian dilakukan seleksi klon. Selanjutnya dilakukan insersi pada intra peritoneal sehingga terbentuk ascites. Hasil penelitian ini menunjukkan bahwa MMP-9 muncul pada 92 kDa dan fragmentasi kolagen tipe IV dengan band 60 – 80 kDa. Untuk menguji reaksi monoklonal antibodi fragmentasi kolagen tipe IV dilakukan uji 12 sampel penderita AMI. Hasilnya semua sampel AMI positif. Kesimpulan menunjukkan bahwa biomarker monoklonal antibodi fragmentasi kolagen tipe IV ternyata bereaksi positif pada penderita AMI.
DIET ATEROGENIK PADA TIKUS PUTIH (Rattus novergicus strain Wistar) SEBAGAI MODEL HEWAN ATEROSKLEROSIS Murwani, Sri; Ali, Mulyohadi; Muliartha, Ketut
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1301.176 KB) | DOI: 10.21776/ub.jkb.2006.022.01.2

Abstract

Atherosclerosis is still a major health problem, because 20% of death in the world is caused by atherosclerosis diseases like stroke and myocardial infarct. One of animal models that was successful in atherosclerosis research was New Zealand white rabbit. The purpose of this preliminary research was to determine the atherogenic diet ofwhite rats (Rattus novergicus strain Wistar) as animal model of atherosclerosis especially to find fixed composition and time of the atherogenic diet taken.This research used male; 2 months age, 150-200 grams body weight of white rats Rattus novergicus strain Wistar. The rats were divided into5 groups in equal number, 4 rats respectively. Theywere a negative control group without diet treatment and 4 groups as treatment groups which were given hipercholesterol diet. Atherogenic diet composed of PAR-S, wheat flour, cholesterol, cholic acid, pork oil and water. Data were obtained by measuring of blood cholesterol level and foam cell formation.
STUDI SEROEPIDEMIOLOGIS CHLAMYDIA PNEUMONIAE DAN BEBERAPA MIKROORGANISME YANG DIDUGA MENYEBABKAN INFARK MIOKARD AKUT Murwani, Sri; Ali, Mulyohadi; Muliartha, Ketut; Purwanto, Purwanto; Susilawati, Ida; Nur Aini, Dinda; Yuni, Yuni
Jurnal Kedokteran YARSI Vol 15, No 1 (2007): JANUARI - APRIL 2007
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.634 KB) | DOI: 10.33476/jky.v15i1.1002

Abstract

Acute myocardial infarction (AMI) is still a major health problem. Approximately 20% of death in the world was caused by atherosclerotic diseases like stroke, myocardial infarct. Recent studies explain the role of infection in the pathogenicity of AMI. The purpose of this study was to examine seroepidemiological study of Chlamydia pneumoniae infection related to AMI and its occurrence as pathogen burden-infection with other microorganisms. Subjects for this study were patients diagnosed clinically and laboratorically as AMI, from RSUD Saiful Anwar and RS. Lavalette,Malang. Blood samples were collected from each subject and serum was analyzed for IgG antibodies against C. pneumoniae, CMV, H. pylori and S. mutans. Clinical status was analyzed to find IMA conventional risk factors, such as smoking, hypertension, hypercholesterolemia, and diabetes mellitus. The result showed that the average age of these patients were 55 years and men were more common than women. All of these patients were infected by microorganism. Patients with infection and conventional risk factors were 51,9%, infection without conventional risk factors was 48,2%, and conventional risk factors for IMA without infection was 51, 85%. The most common conventional risk factor was smoking (37, 0%). Infection by S. mutans was detected in all patients (100%), C. pneumoniae 92,6%, CMV 92,6%, H. pylori 77,8%; however multiple infections were observed in most of the patients. It was concluded that microorganisms played rolesin the pathogenicity of IMA. It was suggested that the development of IMA might be contributed by single infection as well as multiple infections. However, multiple infections tend to be observed in most of the patients.
Co-Authors IDA Susilawati, IDA Mulyohadi Ali Nur Aini, Dinda Purwanto Purwanto Sri Murwani YUNI, YUNI