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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Jurnal Kedokteran Brawijaya MNJ (Malang Neurology Journal) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan YARSI Medical Journal
Mulyohadi Ali
Laboratorium Sentral Biomedik, Fakuktas Kedokteran Brawijaya
Health Professions Medicine & Pharmacology Neuroscience Public Health

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POTENSI EKSTRAK BAWANG DAYAK (Eleutherine sp) SEBAGAI OBAT HERBAL TERSTANDAR (OHT) PADA PENGOBATAN MEDIS Poerwosusanta, Hery; Ali, Mulyohadi; Noor, Zairin; Mintaroem, Karyono; Widjajanto, Edi
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 3, No 2 (2018)
Publisher : Akademi Farmasi ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8.262 KB)

Abstract

Bawang Dayak (Eleutherine sp) merupakan salah satu tanaman yang tumbuh liar di hutan Kalimantan yang berpotensi dikembangkan menjadi Obat Herbal Terstandard. Berdasarkan studi etnofarmakologi, tanaman ini dimanfaatkan masyarakat suku Dayak sebagai obat diabetes, stroke, kanker payudara, antihipertensi, mempercepat penutupan luka, dan pengobatan jantung. Meski sudah digunakan turun temurun dan riset yang berkelanjutan, bawang Dayak belum dimanfaatkan dokter formal untuk pengobatan klinis. Hal ini disebabkan belum adanya uji y akurat dan terstandar yang dapat digunakan sebagai bukti ilmiah. Pada sisi lain, masyarakat berpendapat bahwa penggunaan bawang Dayak sebagai jamu dapat berbahaya karena komplikasi akibat bercampur dengan Bahan Kimia Obat (BKO). Dengan demikian, bawang Dayak perlu diupayakan dalam Program Saintifikasi Jamu untuk mewujudkan jamu, obat herbal terstandar, dan fitofarmaka yang berkualitas, bermutu serta aman. Oleh karena itu, pada artikel ini akan dibahas beberapa riset tentang bioaktifitas bawang Dayak mulai fitokimia hingga tahapan uji praklinik
COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL Trisnawati, Ayu; Anasrulloh, Anasrulloh; Rianawati, Sri Budhi; Khotimah, Husnul; Ali, Mulyohadi; Susetya, Budi
Malang Neurology Journal Vol 5, No 1 (2019): January
Publisher : Malang Neurology Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2019.005.01.2

Abstract

Background: Parkinson disease is characterized with deposition of Lewy Bodies containing α–synuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-like 2 (Nrf2). Centella asiatica  (C.asiatica) contains antioxidant effect, inhibits the aggregation of α–synuclein and improves the locomotor on Parkinson-model animals so it needs to compare to the standard medication.Objective: To compare the C. asiatica extract and Pramipexole to the zebrafish Parkinson model by determining the locomotor activity, α–synuclein expression, and Nrf2.Methods: This study used six groups of zebrafish: negative control, rotenone rotenone [5 μg/L], pramipexole1, 2, 3 (rotenone + pramipexole [3,5] ng/mL, [7] ng/mL, [14] ng/mL), and C. asiatica(rotenone + C. asiatica [10] μg/mL). The observations of locomotor activity of day 0, 14, and 28 were continued to the α–synuclein immunohistochemical examination, and Nrf2 on the midbrain area.Results: There are significant differences in locomotor activity on day 28 among the C. asiatica group with rotenone (p<0,05), while there are no significant differences among the C. asiatica group with pramipexole [7] ng/mL and [14] ng/mL (p>0,05). α–synuclein expression of the C. asiatica group is the lowest and significantly different from all groups (p<0,05), while Nr2 had no significant differences (p>0,05).Conclusion: C. asiatica extract [10] μg/mL is equal to pramipexole [7] ng/mL and [14] ng/mL in improving locomotor activity, but C. asiatica extract holds excellence as it decreases α–synuclein expression better than pramipexole, while Nrf2 expression shows no differences.
EFEK KOMBINASI KLOROKUIN DAN N-ACETYL CYSTEINETERHADAP JUMLAH TROMBOSIT MENCIT GALUR BALB/C YANG DIINFEKSI Plasmodium berghei Mulyastuti, Yuanita; Widijanti, Anik; Ali, Mulyohadi; Iskandar, Agustin; Fitri, Loeki Enggar
Jurnal Kedokteran Brawijaya Vol 20, No 1 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.731 KB) | DOI: 10.21776/ub.jkb.2004.020.01.8

Abstract

Malaria is the most important of the parasitic diseases of humans. In Indonesia, about more than 70 million people live in area which is endemic to malaria, about 15 million new case ofmalaria occurred every year. In malaria immune response forms free radical which assist to eliminate the cause of disease but  also destroys endothelial cells at various organ. This oxidative damage plays an important role in the development of malarial thrombocytopenia. The aim of this research was to investigate the effect of Choroquine combine with N-Acetyl cysteine on the degree of parasitemia and platelets amount during malaria infection. Combination of Chloroquine and N-Acetyl cysteine have been tested by using experimental research method in male Balb/c mice which was infected by Plasmodium berghei. Measurement of the degree ofparasitemia was done everyday and the amount of the platelet as one of the nonspecific cellular immunity parameter at malaria was calculated once in three days. Treatment group were divided to eight groups, group of chloroquine(0,05 mg/gr), group of N-Acetylcysteine with dose 0,25 mg/gr, 0,5 mg/gr, and 1 mg/gr and also combination group of chloroquine and N-Acetyl cysteine with constant dose of chloroquine(0,05 mg/gr) and various dose of N-Acetyl cysteine(0,25 mg/gr, 0,5 mg/gr, and 1 mg/gr). One group consistedof infected mice without treatment as control group.
DIET ATEROGENIK PADA TIKUS PUTIH (Rattus novergicus strain Wistar) SEBAGAI MODEL HEWAN ATEROSKLEROSIS Murwani, Sri; Ali, Mulyohadi; Muliartha, Ketut
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1301.176 KB) | DOI: 10.21776/ub.jkb.2006.022.01.2

Abstract

Atherosclerosis is still a major health problem, because 20% of death in the world is caused by atherosclerosis diseases like stroke and myocardial infarct. One of animal models that was successful in atherosclerosis research was New Zealand white rabbit. The purpose of this preliminary research was to determine the atherogenic diet ofwhite rats (Rattus novergicus strain Wistar) as animal model of atherosclerosis especially to find fixed composition and time of the atherogenic diet taken.This research used male; 2 months age, 150-200 grams body weight of white rats Rattus novergicus strain Wistar. The rats were divided into5 groups in equal number, 4 rats respectively. Theywere a negative control group without diet treatment and 4 groups as treatment groups which were given hipercholesterol diet. Atherogenic diet composed of PAR-S, wheat flour, cholesterol, cholic acid, pork oil and water. Data were obtained by measuring of blood cholesterol level and foam cell formation.
EFFECT OF COMBINED THERAPY USING CHLOROQUINE AND VITAMIN C TO THE PERITONEAL MACROPHAGE FUNCTION IN BALB/C STRAIN MICE INFECTED BY Plasmodium berghei Fitri, Loeki Enggar; Suhendro, Wongso; Murwani, Sri; Muliartha, I Ketut Gede; Ali, Mulyohadi
Jurnal Kedokteran Brawijaya Vol 19, No 3 (2003)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1633.38 KB) | DOI: 10.21776/ub.jkb.2003.019.03.2

Abstract

In acute infection, malaria will induce cellular immunity by activating T lymphocytes and macrophages cells. This induction indirectly triggers free radicals production in order to eliminate the parasites from red blood cells, however high concentration of thismolecules can cause vital tissue pathological changes on host. In late phase of malarial infection, there are immunosupression on macrophages activity including antigen presenting and secretion of immunoregulated mediator. It has been anticipated, vitamin C as antioxidant would diminish the side effect of thesefree radicals during malarial infection and increase the immunity. To see the effect of combination chloroquin and vitamin C in hastening the recuperative process by decreasing parasitemia and increasing the phagocytosis function of macrofages during Plasmodium berghei infection. This study has been carried out using 3 groups of BALB/c mice, all group were inoculated with 1x107Plasmodium berghei infected red-blood cells. No drug was given on control group (IK). In experimental group we introduced an oral therapy ofchloroquin for 5 days in 1.4 mg/cc dosage and vitamin C for 7 days in 0.2 mg/cc dosages concurrently with a Plasmodium berghei inoculation (IKC). One group was only given chloroquin at the same dosage and no drug was given at the control group (IK).
Faktor-faktor yang Berpengaruh pada Akurasi Kode diagnosis di Puskesmas Rawat Jalan Kota Malang Hastuti, Endang Sri Dewi; Ali, Mulyohadi
Jurnal Kedokteran Brawijaya Vol 30, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (727.201 KB) | DOI: 10.21776/ub.jkb.2019.030.03.12

Abstract

Akurasi kode diagnosis dalam rekam medik pasien kasus rawat jalan pada Puskesmas merupakan persyaratan penting sebagai sumber data epidemiologi karena besarnya kontribusi data Puskesmas sebagai layanan primer pada data nasional. Pengamatan dilapangan menunjukkan masih lemahnya akurasi kode diagnosis sesuai ICD-10 sehingaa penelitian inti dilakukan untuk mengeksplorasi keakuratan kode diagnosis penyakit berdasarkan ICD-10, serta menganalisa faktor-faktor mempengaruhi. Kajian cross sectional dilakukan di 5 Puskesmas Rawat Jalan Kota Malang, dengan faktor yang dikaji meliputi pengalaman kerja, pelatihan tentang kodefikasi diagnosis penyakit, ketersediaan buku ICD-10, ketersediaan SOP kode diagnosis dan pengetahuan tentang kodefikasi diagnosis penyakit, dan variabel terikatnya adalah akurasi kodefikasi diagnosis penyakit. Jumlah responden 15 koder terdiri dari 5 tenaga dokter, 5 tenaga dokter gigi dan 5 tenaga perawat yang berasal dari 5 Puskesmas dan kesemuanya terlibat dalam pengkodean diagnosis penyakit di Puskesmas. Jumlah sampel dokumen RM rawat jalan yang diperiksa keakuratannya dari setiap Puskesmas sebanyak 36 dokumen, yang diambil dengan metode purposive sampling. Hasil analisa keakuratan kode diagnosis menunjukkan >50% responden menunjukkan akurasi kodefikasi diagnosis yang rendah (<50%). Hasil uji Fisher's Exact Test secara menunjukkan hubungan signifikan pengalaman kerja mengkode, tersedianya standar operasional prosedur (SOP) kode diagnosis dan pengetahuan tentang kodefikasi diagnosis penyakit, terhadap akurasi kode. Pengetahuan koder tentang kodefikasi diagnosis penyakit merupakan faktor yang paling berperan terhadap keakuratan kodefikasi diagnosis penyakit.
Efek Ekstrak Propolis terhadap Ekspresi TNF-α, Apoptosis dan Nekrosis Jaringan Otak Tikus Model Traumatic Brain Injury (TBI) Dewi, Ashalia Chandra; Ali, Mulyohadi; Purnomo, Hari
Jurnal Kedokteran Brawijaya Vol 29, No. 2 (2016)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2016.029.02.4

Abstract

Ekstrak propolis merupakan produk lebah dengan berbagai kandungan aktif yang memiliki efek antioksidan, antiinflamasi, proteksi DNA, neuroprotektan dan immunomodulator. Propolis mampu menurunkan reaksi inflamasi melalui hambatan terhadap aktivitas TNF-α dan NF-κB, mampu menurunkan Bax dan caspase-3, dan meningkatkan Bcl-2, yang berperan pada jalur apoptosis. Propolis berpotensi sebagai terapi alternatif dalam menurunkan inflamasi, apoptosis dan nekrosis pada traumatic brain injury (TBI) via modulasi ekspresi TNF-α. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak propolis dalam berbagai dosis pada ekspresi TNF-α, apoptosis dan nekrosis pada jaringan otak tikus model TBI. Sampel dibagi menjadi 5 kelompok, yaitu: kontrol negatif, kontrol positif, kelompok model trauma dan diberi perlakuan propolis masing-masing dosis 50, 100, dan 200mg/kgBB/h. Pada akhir penelitian, tikus dikorbankan dan dibuat preparat otak untuk menilai ekspresi TNF-α, jumlah sel apoptosis dan area nekrosis. Ekspresi TNF-α, apoptosis dan nekrosis lebih rendah pada kelompok TBI yang mendapatkan ekstrak propolis 50, 100, dan 200mg/kgBB/h (p=0,000). Penelitian ini membuktikan bahwa ekstrak propolis berpengaruh dalam penurunan ekspresi TNF-α, apoptosis dan nekrosis jaringan otak tikus model TBI.Kata Kunci: Apoptosis, ekstrak propolis, ekspresi TNF-α, nekrosis, traumatic brain injury
STUDI SEROEPIDEMIOLOGIS CHLAMYDIA PNEUMONIAE DAN BEBERAPA MIKROORGANISME YANG DIDUGA MENYEBABKAN INFARK MIOKARD AKUT Murwani, Sri; Ali, Mulyohadi; Muliartha, Ketut; Purwanto, Purwanto; Susilawati, Ida; Nur Aini, Dinda; Yuni, Yuni
Jurnal Kedokteran YARSI Vol 15, No 1 (2007): JANUARI - APRIL 2007
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.634 KB) | DOI: 10.33476/jky.v15i1.1002

Abstract

Acute myocardial infarction (AMI) is still a major health problem. Approximately 20% of death in the world was caused by atherosclerotic diseases like stroke, myocardial infarct. Recent studies explain the role of infection in the pathogenicity of AMI. The purpose of this study was to examine seroepidemiological study of Chlamydia pneumoniae infection related to AMI and its occurrence as pathogen burden-infection with other microorganisms. Subjects for this study were patients diagnosed clinically and laboratorically as AMI, from RSUD Saiful Anwar and RS. Lavalette,Malang. Blood samples were collected from each subject and serum was analyzed for IgG antibodies against C. pneumoniae, CMV, H. pylori and S. mutans. Clinical status was analyzed to find IMA conventional risk factors, such as smoking, hypertension, hypercholesterolemia, and diabetes mellitus. The result showed that the average age of these patients were 55 years and men were more common than women. All of these patients were infected by microorganism. Patients with infection and conventional risk factors were 51,9%, infection without conventional risk factors was 48,2%, and conventional risk factors for IMA without infection was 51, 85%. The most common conventional risk factor was smoking (37, 0%). Infection by S. mutans was detected in all patients (100%), C. pneumoniae 92,6%, CMV 92,6%, H. pylori 77,8%; however multiple infections were observed in most of the patients. It was concluded that microorganisms played rolesin the pathogenicity of IMA. It was suggested that the development of IMA might be contributed by single infection as well as multiple infections. However, multiple infections tend to be observed in most of the patients.
EFEK ISOFLAVON FITOESTROGEN DARI EKSTRAK Pueraria lobata TERHADAP MEMORI DAN AKTIVITAS KOLINERGIK DI HIPPOKAMPUS CA1 PADA TIKUS HIPOESTROGEN Safithri, Fathiyah; Ali, Mulyohadi
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 6, No 2 (2010): Desember 2010
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/sm.v6i2.1020

Abstract

Data from retrospective and case-conttrole showed that estrogen replacement therapy (ERT) could prevent ang delayed againstAlzheimer Disease (AD). The using of ERT is still controversial because of their long-term side effect. Isoflavon as a part of phytoestrogenhas potential effect as an alternative substitute for ERT. The aim of this study is to examine the effect of phytoestrogen from Pueraria lobataextract in increasing memory’s function and whether it occur by the increased of cholinergic activity in CA1 hippocampus. Exploration studyperformed by three step. First, determined genistein and daidzein concentration in Pueraria lobata extract, as basic of dose determining.Second, oophorectomy performed to made a hypoestrogenic rat’s model; and third, determined method memory’s function in rats. This studyused twenty five female Rattus novergicus strain Wistar, 10 -11 weeks of age, five rats were normal rats (N) as first group and twenty ratswere hypoestrogenic rats for 2nd, 3rd, 4th, and 5th groups. All of the hypoestrogenic rat were devided into 4 groups, each 5 rats. Hypoestrogenicrats group without Isoflavon Genistein Daidzein supplement (OVX), hypoestrogenic rats group with Isoflavon Genistein Daidzeinsupplement (OVX+IGD) dosage of 15, 30, and 60 mg/kgweight/day each. The IGD was given for 21 days. The Morris Water Morris(MWM) test was performed in 17th, 18th, 19th, 20th, and 21st day. On 22nd day rats were killed. Frozen section of the brain was performedimmediately. Cholinergic activities on CA1 hippocampus were examined with AChE staining. A significant difference was found onmemory’s function and cholinergic activities on CA1 hippocampus between OVX and OVX+IGD groups at all doses (p value = 0,000 ofeach). No significant difference on IGD effect toward brain weight (p=0,936) and pyramidal cell number on CA1 hippocampus (p=0,961).It was suggest that increased memory’s function on OVX+IGD groups were caused by the increased of cholinergic activities on CA1hippocampus.Key word : Isoflavones; phytoestrogen; Genistein; Daidzein; Pueraria lobata; Memory; cholinergic.
Co-Authors Agustin Iskandar Anak Agung Gede Sugianthara Anasrulloh, Anasrulloh Anik Widijanti Dewi, Ashalia Chandra Edi Widjajanto Fathiyah Safithri HARI PURNOMO Hastuti, Endang Sri Dewi I Ketut Gede Muliartha IDA Susilawati, IDA Karyono Mintaroem Ketut Muliartha Loeki Enggar Fitri Nur Aini, Dinda Poerwosusanta, Hery Purwanto Purwanto Sri Budhi Rianawati, Sri Budhi Sri Murwani Susetya, Budi Trisnawati, Ayu Wongso Suhendro Yuanita Mulyastuti YUNI, YUNI Zairin Noor