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All Journal JBIO: jurnal biosains (the journal of biosciences) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Borneo Journal of Medical Laboratory Technology Jurnal Kesehatan Rajawali Asian Journal of Health and Applied Sciences
NI'MATUL MURTAFI'AH
Institut Kesehatan Rajawali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Aktivitas Perombakan Selulosa Dan Pengendapan Logam Mn Pada Konsorsium Bakteri Pereduksi Sulfat Ni’matul Murtafi’ah
Jurnal Kesehatan Rajawali Vol 9 No 1 (2019): Maret
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54350/jkr.v9i1.7

Abstract

Latar Belakang: Erupsi Gunung berapi mengakibatkan terjadinya pencemaran lingkungan akibat penyebaran abu vulkanik. Abu vulkanik gunung berapi mempunyai unsur salah satunya berupa sulfat dan logam berat. Kandungan sulfat dan logam berat yang tinggi mengakibatkan pH bersifat asam. Tujuan Penelitian: untuk mengetahui pengaruh kadar serbuk gergaji Acacia mangium terhadap aktivitas dan karakter bakteri pada konsorsium Bakteri Pereduksi Sulfat dalam mereduksi sulfat dan logam Mn dengan partikel zeolit pada skala batch culture. Metode Penelitian: Penelitian dilaksanakan dengan metode biologi menggunakan Bakteri Pereduksi Sulfat (BPS) secara batch culture menggunakan bioreaktor menggunakan media Postgate B dengan konsentrasi sulfat 100 ppm dan Mn 10 ppm pada pH 4. Metode analisis data menggunakan Rancangan Acak Lengkap (RAL), perlakuan yang diberikan yaitu pemberian zeolit sebesar 20 gr/L sedangkan kadar serbuk gergaji sebesar 0%;1,25%; 2,5% dan 5%. Hasil: Hasil penelitian batch culture menunjukkan serbuk gergaji kayu akasia berpengaruh sangat nyata terhadap perubahan pH. Pemberian serbuk gergaji mengkatkan pH menjadi 6,5-6,91. meningkatkan Kadar serbuk gergaji 2,5% yang paling optimal meningkatkan effisiensi reduksi sulfat sebesar 63,90% dan effiensi reduksi Mn sebesar 54,80%. Simpulan: Serbuk gergaji Akasia dapat meningkatkan aktivitas reduksi sulfat dan logam Mn. Aktivitas konsorsium BPS bekerja secara optimal mereduksi konsentrasi sulfat dan logam Mn dengan penambahan kadar serbuk gergaji sebesar 2,5%.
ISOLASI BAKTERI SELULOLITIK PADA KONSURSIOM BAKTERI PEREDUKSI SULFAT DENGAN PENAMBAHAN SERBUK GERGAJI KAYU AKASIA Ni'matul Murtafia'ah
Jurnal Kesehatan Rajawali Vol 9 No 2 (2019): September
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54350/jkr.v9i2.37

Abstract

Latar Belakang: Pencemaran lingkungan menjadi salah satu masalah bagi kesehatan manusia. Pencemaran lingkungan dapat menurunkan pH yang dapat mempengaruhi proses fisiologi dan molekuler dengan menonaktifkan enzim, menggantikan unsur penting sehingga mengganggu integritas membran. Penurunan pH mengakibatkan peningkatan kandungan sulfat pada perairan sehingga mempengaruhi kestabilan ekosistem.Tujuan: untuk mengetahui karakter bakteri selulolitik pada konsorsium Bakteri Pereduksi Sulfat dalam mereduksi sulfat pada skala batch cultureMetode: : Penelitian dilaksanakan dengan metode bioremidiasi menggunakan Bakteri Pereduksi Sulfat (BPS) skala batch culture menggunakan bioreaktor menggunakan media Carboxymethyl cellulose (CMC). Konsorsium yang digunakan yaitu konsorsium yang efektif mereduksi logam Mn. Metode analisis data menggunakan Rancangan Acak Lengkap (RAL), perlakuan dengan pemberian zeolit sebesar 20 gr/L sedangkan kadar serbuk gergaji sebesar 0%;1,25%; 2,5% dan 5%.Hasil: Hasil inokulasi bakteri selulolitik melalui konsorsium BPS pada medium Postgate B yang di inokulasikan pada medium CMC yang paling optimal yaitu pada perlakuan Zc3KK1 pada pengenceran 10-2 dengan kode isolat FKK1 luas diameter zona bening 26,67mm dengan diameter koloni 5 mm.Kesimpulan: Bakteri dapat menghidrolisis selulosa baik secara aerob maupun anaerob yang ditunjukan terbentuknya zona bening dengan diameter maksimum sebesar 26,67 mm dengan penambahan serbuk gergaji 2,5%. Besar kecilnya ukuran zona bening dipengaruhi oleh aktivitas spesifik enzim di daerah amorf pada substrat menyebabkan CMC terhidrolisis secara efisien.
PENGARUH TINGKAT KEPATUHAN SUMBER DAYA MANUSIA TERHADAP MUTU INTERNAL PELAYANAN LABORATORIUM DI PUSKESMAS KABUPATEN GARUT Fitri Rahmi Fadhilah; Ni'matul Murtafia'ah
Jurnal Kesehatan Rajawali Vol 11 No 1 (2021): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.271 KB)

Abstract

Compliance with human resources, in this case health workers, greatly affects the quality of health servicesin providing health services, therefore all health service processes are expected to refer to the establishedstandard operating procedures. Internal Quality Consolidation is a laboratory system to identify andminimize errors from Pre-Analytical, Analytical and Post-Analytical. This type of research aims todetermine the relationship between the level of compliance of human resources with the internal quality oflaboratory services at the Garut district health center. The method of this research is qualitative by usingthe analytical research method of rank sperman correlation with a total of 30 respondents conducted at thePuskesmas Laboratory in Garut district. The results of this study indicate that there is a relationship of 0.378between the level of compliance of human resources with the internal quality of laboratory services at theGarut district health center, with a level of closeness as evidenced by the coefficient of determination,namely 14.30%, the level of compliance of human resources with the internal quality of laboratory services.has a significant effect with p-value 0,040. The conclusion of this study is that there is a significant effectbetween the level of compliance of human resources to the internal quality of laboratory services at theGarut district health center.
THE EFFECT OF ZEOLITE ADMINISTRATION IN THE CONSORTIUM OF HEAVY METAL REDUCING BACTERIA Pb ON LIQUID WASTE OF TEXTILE INDUSTRY Ni'matul Murtafi'ah; Suci Rizki Nurul Aeni; Erika Setyowati
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 1 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i1.36353

Abstract

Textile industry wastewater treatment must be managed properly so as not to cause environmental pollution that has an impact on human health. Industrial wastewater treatment by utilizing microbial activity with the addition of zeolite with varying concentrations measured using UV-Vis Spectrophotometry. This study aims to find out the effect of variations in zeolite concentration on bacterial consortiums in reducing lead heavy metals (Pb) and find out the characteristics of bacteria reducing heavy metals Pb after added zeolite. The results of the study using UV-Vis Spectrophotometry showed that the concentration of zeolite 3% was able to reduce heavy metals Pb on the 14th day with consecutive levels of 0.473 with an initial lead content of 2,460 ppm. The most significant decrease occurred in the addition of a zeolite concentration of 3%. This is shown in its effectiveness value of 86.772%. From this study, it can be concluded that the administration of a concentration of 3% in a consortium of bacteria can lower the levels of heavy metals Pb. Based on the characteristics of bacteria in biochemical tests, namely the confectionery test (Maltose, Glucose, Sucrose) obtained positive results, negative Lactose test, red /red TSIA test, negative marker test, and positive. Basil-shaped gram-negative bacteria refer to the genus Desulfobacter.
Identifikasi Bakteri Pereduksi Logam Pb dalam Bioremediasi Sampel Air Sungai Citarum Menggunakan Analisis Gen 16s rRNA: Identification of Pb Metal Reducting Bacteria in Bioremediation from Citarum River Water Samples Using 16s rRNA Gene Analysis Ni'matul Murtafi'ah; Suci Rizki Nurul Aeni
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5102

Abstract

The bioremediation technique is an effort to remove the heavy metal Pb in samples of Citarum river water. High concentrations of heavy metals harm the environment, so Pb metal-reducing bacteria are needed as a pollution solution. Bioremediation uses bacteria. Bacteria are identified by molecular methods, namely sequencing analysis techniques, because they are more accurate. To determine the nucleotide sequence of Pb metal-reducing bacteria isolates and which bacterial species can reduce Pb metal. The methodology used is primary data collection, namely conducting research directly, namely the stages of bacterial purification through gram staining techniques, pure colonies, then proceeding to the DNA isolation stage. The isolated DNA is then electrophoresed. The electrophoresis results are then purified and proceed to the sequencing stage. The feasibility test results were to obtain pure DNA from the reducing bacteria. The next step was PCR amplification of the 16S rRNA gene of Pb-reducing bacteria. The research results started at the DNA isolation stage and obtained a purity of 1.80. The PCR results were in the form of amplification of the 16S rRNA gene of Pb-reducing bacteria. Electrophoresis obtained the results of DNA bands measuring ± 1400 bp—phylogenetic tree analysis with 99.60% relatedness to Bacillus cereus, which can reduce Pb. Bacillus cereus species can carry out detoxification mechanisms against the toxic effects of Pb metals, such as—biosorption, bioaccumulation, reduction, solubilization, precipitation (formation of extracellular complexes), and methylation.
Antibacterial effect of corarian seed (Coriandrum sativum L.) extract on Streptococcus mutans Kodariah, Liah; Ghasani, Firzanah; Murtafi'ah, Ni'matul
Asian Journal of Health and Applied Sciences Vol. 1 No. 1 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.645 KB) | DOI: 10.53402/ajhas.v1i1.7

Abstract

Dental plaque plays an important role in causing dental caries. In plaque formation, gram-positive coccus bacteria are found, one of which is Streptococcus mutans which can secrete toxins so that tooth cells are damaged. Dental plaque or dental caries can be controlled in number by controlling plaque with antimicrobial agents such as mouthwash. However, the continuous use of antimicrobials can cause side effects for users, including hypersensitivity reactions and resistance, so it is necessary to use natural ingredients as antibacterial alternatives. Therefore, this study used coriander seeds as an alternative to inhibit the growth of Streptococcus mutans bacteria. The purpose of this study was to determine the effectiveness of coriander seed extract (Coriandrum sativum L.) with varying concentrations on the growth of Streptococcus mutans bacteria. This study used the diffusion method as a bacterial test method for coriander seed extract. The concentrations tested in this study were 3%, 6% and 9%. Based on the results of the study showed that there was an inhibition zone in the test of coriander seed extract against Streptococcus mutans. At a concentration of 3% the average inhibition zone was 3.7 mm, the concentration of 6% was 4.7 mm and concentration of 9% by 6.06 mm. The results of this study concluded that coriander seed extract (Coriandrum sativum L.) was effective in inhibiting the growth of Streptococcus mutans bacteria.
Test of The Inhibitory Power of Lemongrass Extract Against The Growth of Candida albicans On Natural Resources Medium Murtafi'ah, Ni'matul; Syarofah, Zela Adeline; Pitono, Arie J.
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.59

Abstract

Candidiasis is an acute or chronic inflammation that causes disorders of the body's system caused by the fungus Candida albicans. Candida albicans is a common cause that does not cause disease in a person's normal immune system but can attack a person's poor system. Candidiasis can be treated with antifungal drugs, but prolonged and extensive use of antifungal drugs causes side effects. So alternative drugs are needed that can reduce these side effects. Lemongrass (Cymbopogon citratus) has pharmacological activity as an antifungal. Research Objectives: To determine the inhibition of lemongrass extract on the growth of Candida albicans in an SDA medium with concentrations of 0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The study used a descriptive research design. The method of testing the resistance is carried out by the good diffusion method. Lemongrass extract can inhibit the growth of Candida albicans fungus by forming an inhibition zone. At a concentration of 0% lemongrass extract, no inhibition zone was formed, a concentration of 0.2% formed an inhibitory zone of 10.5 mm, a concentration of 0.4% formed an inhibition of 6.1 mm, a concentration of 0.6% formed an inhibition zone of 14 mm, the concentration 0.8% formed an inhibition zone of 10.8 mm, a concentration of 15 formed an inhibitory zone of 27.6 mm.
Molecular identification of the bacterium acute conjunctivitis by the method of sequensing gen 16S rRNA Murtafi'ah, Ni'matul; Kafesa, Ally; Wahid, Aziz Ansori
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 1 (2024): Advancements in Biomedical Research: Insights from Medical Laboratory and Technol
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i1.5207

Abstract

Conjunctivitis is the most common eye disease, characterized by contextual inflammation, which can be caused by bacteria. The diagnosis of conjunctivitis is established based various factors, including the patients’s medical history, onset of eye symptoms, non-ocular symptoms, previous disease history, family medical history, allergies, and physical examination of the eyes, which may include assessments of visual acuity and vision field. Some cases of conjunctivitis require laboratory examinations to confirm the diagnosis. These may include cytological examination with Giemsa staining, Chlamydia Diagnostic Test, Nucleic acid amplification tests (NAATs) or Polymerase chain reaction (PCR), and microbiological tests. Microbiological examination helps identify the bacteria responsible for conjunctivitis and aids in treatment by prescribing antibiotics to suppress the growth of the infecting bacteria. While identification tests are not routinely performed, researchers often seek to determine the specific type of bacteria causing conjunctivitis infections, which may require several laboratory tests. Bacterial virulence plays a significant role, with genetic mutations potentially leading to severe infections of varying severity. Virulence genes encode proteins that express pathogenic properties. The species responsible for conjunctivitis can be definitively identified definitively through microbiological examination, utilizing methods such as the 16S Ribosomal RNA Sequencing (rRNA) technique, known for its accuracy and speed. This study aims to analyze the results of rRNA sequencing in cases of acute bacterial conjunctivitis caused by 16S rRNA genotyping. The research employed an exploratory metholodolot, with the results analyzed using the The Basic Local Alignment Search Tool (BLAST) tracking program database on the National Center for Biotechnology Information (NCBI) website. The findings revealed that Sphingomonas paucimobilis encoded the 16S rRNA using Universal Primary 27 F and 1492 R, obtained in a sequence size of 1351bp. The isolate demonstrated similarities to Sphingomonas paucimobilis.
Co-Authors Arie J. Pitono Erika Setyowati Fitri Rahmi Fadhilah Ghasani, Firzanah Kafesa, Ally Kodariah, Liah Suci Rizki Nurul Aeni Syarofah, Zela Adeline Wahid, Aziz Ansori