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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY
S. Murtini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary Other

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The Clinical Symptom and Anatomical Pathology of Tolaki Chicken which Infected Newcastle Disease Viral Pagala, M. A.; Muladno, Muladno; Sumatri, C.; Murtini, S.
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

This study aim was observatiion the clinical and anatomic pathology Tolaki chicken infected virus Newcastle Disease (ND) as an affirmation analysis of the cause of the viral disease. A total of 30Tolaki chickens divided into 3 groups based on the Mx (Myxovirus) gene genotype. Mx gene is a genethat is antiviral, producing three genotypes (AA, AG and GG), respectively 10 chicks kept in separatecages. Challenge test performed by infection with a virus gene ND VII eye drops at a dose of 104CLD50 /0.5 ml / head. The results showed that in the first week of chicken genotype AA, AG and GGgenerally show the same clinical symptoms such as ducking, breakaway, face swollen, snoring.Chicken looks decreased appetite and green diarrhea are found in the feces. The number of chickensthat died at the AA and AG there are three tails, while the GG Chicken found 6 tail. In the secondweek the AA genotype was not found green diarrhea, while the AG and GG discovered greendiarrhea. The number of chickens that died at the AA and AG there are two tails, while GG was founddead three tails. Total chickens that died at the AA and AG found as many as 5 tails, while the GG asmany as 9 tails. Generally the infected chicken ND virus causes swelling of the spleen,bleeding/redness of the intestine and trachea in all genotypes. Swelling of the spleen in the AAgenotype was found to be 20%, AG and GG 60% of 100%. Spleen were obtained blackish color on theGG genotype of 44.44%. Based on changes in anatomic pathology in this study was obtainedcharacteristics ND diseased chickens, so the chickens have been infected with the virus diagnosedND. Patognomosis changes in anatomical pathology characterized by hemorhagi enteritis in thegastrointestinal tract (gut) and respiratory tract (trachea). Based on the results of the ND viruschallenge test to prove the group challenged chickens have been infected with ND virus. ND virusinfection attacks the intestinal organs, trachea, and spleen affecting the decline of the immunesystem in all genotypes. AA and AG genotypes have better protective properties against ND virusinfection than genotype GG.Key Words: Clinical Symptom, Anatomy Pathology, Tolaki Chicken, and ND Viral
Produksi kolostrum antivirus avian influenza dalam rangka pengendalian infeksi virus flu burung A. Esfandari; I. WT. Wibawan; S. Murtini; SD. Widhyari; B. Febram
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

This experiment was conducted to study the prospect of bovine colostrum utilization to produce specific antibody as passive immunotherapy against avian influenza. Pregnant Frisian Holstein cows were injected with commercial killed Avian Influenza (AI) vaccine given double doses subcutaneously three times every two weeks. Prior to vaccination, the cows were given immunomodulator 0.1 mg.kg-1 BW administered orally for three days. The animals then were injected by inactive H5N1 antigent without adjuvant intravenously to meet the dose of 104 HAU. Blood samples were collected to detect anti AI antibody using Enzyme Linked Jmmunosorbent Assay technique. Colostral samples were analysed to detect antibody against AI using Haemagglutination Inhibition technique. IgG stabilities were tested against enzyme, pH, and spray dried prosessing with inlet dan outlet temperature of 1400C and 520C.repectively. The colostral lgG efficacy on neutralizing H5N1 virus activity was determined in vitro (by using Serum Neutralization Test and protective titer measurement) and in ovo (challenge test by using Embryonic Chicken Egg). The result indicated that serum antibody against H5N1 was detected one week after the second vaccination. Titer of colostral antibody against H5N1 was high (28 ). Biological activity of colostral IgG remain stable at pH 5-7 and after spraying-drying prosessing, but decreased after treatment by trypsin and pepsin enzymes. The neutralization test showed that the fresh and spray dried colostral IgG against H5N1 were able to neutralize 107 EID50 AI virus H5N1 with neutralization index of 1.1 and 1.0, respectively. In conclusion, pregnant Frisian Holstein cows injected with commercial killed Avian Influenza (AI) vaccine were able to produce colostral lgG against AI H5Nl. Keywords: avian influenza, bovine colostrum, lgG, passive immunotherapy
Weight Performance of 4th Generation IPB-D3 Local Chickens Aged 1-3 Months and It`s Heritability Value I. Galib; C. Sumantri; S. Darwati; S. Murtini
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 12 No. 1 (2024): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.12.1.14-20

Abstract

Bogor Agricultural University local chicken which named IPB-D3 is a prospective male line obtained from the chicken selection program IPB-D1. The establishment of IPB-D3 chickens aims to produce local chickens with fast growth weight. This research aims to evaluate the performance of local chickens from IPB-D3 Generation 4 (G4) and its heritability values. The observed samples include 10 males and 30 females of IPB-D3 chickens of Generation 3 (G3) aged 21-40 weeks as parent and 100 Day Old Chickens (DOC) IPB-D3 G4 up to 12 weeks old. The results obtained that IPB-D3 G4 chickens show an average body weight for males 1021.44 g and females around 648.33 g at the age of 12 weeks. The feed given to IPB-D3 G4 chickens is the same as the parents, namely it has nutrients below commercial feed because the feed given uses a mixture of local feed which are bran and corn which already used in Sinar Harapan Farm Sukabumi. Weight gain is the change in body weight that occurs over a specific period, and the weight gain of IPB-D3 G4 chickens is relatively fast, especially from the 2nd to the 3rd month, with a weight gain rate reaching 406.27 g. Growth rate from IPB-D3 G4 chickens experiences a slowdown at the age of 2 months and falls below their optimal growth rate. Another aspect to report is the mortality rateof IPB-D3 G4 chickens, which is quite high at the DOC and female age due to environmental conditions and disease attacks. However, the mortality rate decreases with the increasing age of the chickens, and no deaths are found in 12 weeks old male chickens. The heritability value of body weight in IPB-D3 G4 chickens is high at the age of 4 weeks and can be used as a basis for selection based on body weight.
Pemberian Anti-Myostatin pada Induk Serta Keragaman Gen Myostatin dan Asosiasinya terhadap Potongan Karkas Anak Ayam Sentul (F1) Fastawa, R.; Sumantri, C.; Gunawan, A.; Murtini, S.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 7 No. 1 (2019): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

Myostatin (MSTN), a member of the transforming growth factor β (TGF-β) superfamily, is a negative regulator of skeletal muscle growth. Improving muscle growth is very important to increase the economic value of Indonesian indigenous chicken especially in sentul chicken. This study was aimed to identify the polymorphism of the Myostatin gene in sentul indonesian indigenous chicken with maternal immunization against Myostatin  on post hatch and to evaluate its effect on carcass traits. In this study, 48 Sentul hens for up to 6 months have been divided into three groups with 16 birds in each group: CON, KLH, and KLH-MSTN. The CON group has not received immunization, the KLH group has been immunized with 1 mg of KLH, and KLH-MSTN has been immunized with 1 mg of KLH-MSTN conjugate intramuscularly. The effects of each group have been observed on F1 carcass production.The gene polymorphism was identified using PCR-RFLP and the effect of genotype on carcass chacteristics were analyzed using SAS General Linear Model Procedure. The product of amplification was 247 bp. This  locus was polymorphic in sentul chicken with two alleles (G and T) and three genotypes (GG, GT, TT). Association analysis were not significant in carcass characteristics. The result of this study showed that interaction of each group with genotype not significant on carcass weight of F1 chicken sentul each age group. F1 sentul chicken 12 week-old significant association of genotype was observed only in thighs weight while 20 week-old was significant in live weight, carcass weight, breast weight, wings weight, thighs weight, drum sticks weight, breast muscle weight, thighs muscle weight and drum sticks muscle weight. These results suggest that no effect genotype on maternal immunization against myostatin to stimulate carcass characteristic in sentul chickens.
Keragaman Gen NRAMP-1 dan INOS pada Ayam Sentul Seleksi Ardiyana, M.; Sumantri, C.; Murtini, S.; Sartika, T.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 6 No. 2 (2018): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

NRAMP-1 protein and INOS enzyme have been shown as deffense mechanism against bacteria and virus infection. These genes have not been investigated in Sentul Selection chickens. This study was aimed to determine the polymorphism of NRAMP-1 and INOS in Sentul Selected chickens. A number of 172 sentul selected chickens was observed for NRAMP-1 and INOS genes using PCR-RFLP. The frequency of genotypes and alleles, polymorphic information content and Hardy-Weinberg equilibrium status (HWE) were analyzed. Polymorphism in NRAMP1|SacI and INOS|AluI were observed. The population was in HWE for both genes. NRAMP1 gene frequencies had higher TC genotypes and INOS gene frequencies had higher CC genotypes (P<0.05) in Selected Sentul chickens. Furthermore, the NRAMP1 and INOS genes, the frequency of allele C was higher than the T allele. It could be concluded that NRAMP-1 and INOS gene has potential to propose has associated with immune traits in Sentul chicken.
Asosiasi c.248 A>G gen CD1B dengan IgY dan Titer ND pada Ayam Sentul Galur Sensi-1 Agrinak M. F. Al-Habib; S. Murtini; A. Gunawan; N. Ulupi; C. Sumantri
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 8 No. 1 (2020): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.8.1.30-35

Abstract

The aim of this study was to identify the polymorphism of the CD1B gene in c.248 in exon 3 and its association with IgY and ND titers in sentul chicken Sensi-1 Agrinak line. IgY is the main antibody of chickens while the ND titer is an ND specific antibody, both of which have an important role in the response of disease resistance. The Cluster of differentiation 1B (CD1B) gene is one of the major genes of disease resistance that functions in representing antigens through specific immune responses. This study used 17 sensi-1 chicken lines. The study was conducted by PCR sequencing method, ELISA test, and HI test. The result showed mutation on c248 A>G in exon 3. The SNP were polymorphic and Hardy Weinberg equilibrium. Thre were two A and G alleles and 3 genotypes namely AG, GG, and AA. The frequency of the GG genotype was higher (0.41) compared to other genotypes. The AG genotype were associated with IgY production and were not associated with ND titers.
Keragaman Gen BG1 sebagai Kandidat Gen Penciri Ketahanan Penyakit pada Calon Galur Ayam IPB-D2 N. N. Miraj; C. Sumantri; S. Murtini; N. Ulupi
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 10 No. 3 (2022): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.10.3.144-151

Abstract

The BG1 gene is a disease resistance candidate gene in chicken, which is located in the MHC region. The mechanism of the BG1 gene is known as an inhibitor of T lymphocyte cell activation. This study aims to identify the diversity of the SNP gene BG1 in the IPB-D2 chicken line. IPB-D2 chicken is a candidate for a new line from a cross from IPB-D1 chicken, which was selected for high body resistance and good productivity. A total of 27 DNA samples of IPB-D2 (G0) chickens and 58 samples of IPB-D2 (G2) chickens were sequenced to identify the diversity of BG1 genes. The study found 6 point mutations (g.4489 G>T, g.4712 C>T, g.4741 T>C, g.4747 T>C, g.4749 T>C, g.4761 G>A) and deletion of 2 nucleotide bases g.4758 - g.4759 delAT. The combination of 6 SNPs forms 4 haplotypes. The SNPs that changed the amino acid composition were SNP g.4489 G>T cys> phen, g.4712 C>T gln>codon stop, g.4749 T>C val>ala and g.4761 G> A gly> glu. All mutations are classified as polymorphic, low heterozygosity value, and not in Hardy-Weinberg equilibrium.
Production Performance of Debu and Kelabu Sentul Chicken at Different Igy Concentrations A. U. Khairiyah; C. Sumantri; S. Murtini; A. Anang
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 11 No. 2 (2023): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.11.2.73-79

Abstract

Sentul chicken is one of Indonesia’s native chickens that has high potential for meat and egg production performance. The production of Sentul chicken breeds with high body resistance is one of the solutions to fulfill this potential. This research aimed to ascertain the impact of various IgY concentrations (high, medium, and low) on the production of 90 Debu and 90 Kelabu Sentul chickens. Parameters observed included pre-laying and laying performance. Six replications of a completely randomized design were used to conduct the experiment. The Anova test was used to analyze the data that was collected. The findings revealed that different strains and sex of Debu and Kelabu Sentul chickens did not significantly affect the value of IgY concentration. In the entire population studied, only 12.09% of Debu Sentul chickens and 11. 32% of Kelabu Sentul chickens had low Igy concentration values. In pre-laying period, the difference in the types of chicken strains has no significant effect on the body weight gain and thefeed convertion. Chickens with high IgY concentrations performed worse during the laying phase than hens with low IgY concentrations. The research found that the population of Kelabu Sentul chickens with high IgY concentration value is more than Debu Sentul chickens, which makes them suitable to be used as breeders.
Co-Authors A. Anang A. Esfandari A. GUNAWAN A. U. Khairiyah Ardiyana, M. B. Febram C. SUMANTRI C. Sumantri Fastawa, R. I. Galib I. WT. Wibawan M. F. Al-Habib Muhammad Amrullah Pagala N. N. Miraj N. Ulupi S. Darwati SD. Widhyari Sumatri, C. T. Sartika