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All Journal Indonesian Journal of Cancer Chemoprevention International Journal of Educational Research Excellence (IJERE)
Naufa Hanif
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
Arts Humanities Biochemistry, Genetics & Molecular Biology Education Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Caesalpinia sappan L. Ethanolic Extract Decrease Intracellular ROS Level and Senescence of 4T1 Breast Cancer Cells Naufa Hanif; Adam Hermawan; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 10, No 1 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss1pp16-23

Abstract

Highly and uncontrolled cell proliferation on cancer cells may boost ROS level intracellular accumulation up significantly. Sappan heartwood (Caesalpinia sappan L.) have been known to have cytotoxicity effect toward several cancer cells. This research was conducted to develop Caesalpinia sappan L. heartwood ethanolic extract (CSE) as chemopreventive agent which can be used as cancer therapy, looked from antioxidant and anti-senescence activity toward 4T1 breast cancer cell. CSE was obtained through maceration using ethanol 70% as solvent. Cytotoxicity activity of CSE was done by using MTT assay with IC50 as parameter. This IC50 was used as basic to the next assay, ROS assay by using DCFDA staining flowcytometry to look ROS level intracellular of CSE toward 4T1 cell and senescence assay by using senescence associated β-galactosidase (SA β-gal) assay with % cell senescence as their parameter. CSE toxic to 4T1 cell that proved from IC50 value of 25 µg/mL. Single treatment of CSE on concentration 12,5; 25; and 37.5 µg/mL able to suppress ROS level intracellular. If compared with untreated cell, single treatment of CSE on concentration 6 and 12 µg/mL showed % cell senescence not significantly different. Based on this result, CSE is cytotoxic, has antioxidant and antisenescence activity, so it has great potential to developed as chemoprevention agent on cancer therapy.Keywords : Caesalpinia sappan L., 4T1 breast cancer line, Reactive Oxygen Species (ROS), anti-senescence
The Religious Practices of Indonesian Students Studying in Turkey Dalmi Iskandar Sultani; Cita Ayni Putri Silalahi; Sujarwo; Dinil Abrar; Naufa Hanif; Suci Amanda Putri; Fathin Nazirah Aulia
International Journal of Educational Research Excellence (IJERE) Vol. 3 No. 2 (2024): July-December
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijere.v3i2.933

Abstract

The practice of religion for Indonesian students studying in Turkey deserves the attention of academics. This is because conditions, student backgrounds, distance and time differences between two countries can be factors in the fluctuations in the practice of religion for students. This research aims to prove whether Indonesian students in Turkey still consistently implement Islamic teachings. This research uses a qualitative research method with a case study approach to obtain in-depth data and information findings. The data collection technique used was interviews. The interview aspect is an important factor for student informants, to explore in detail and in depth about religious practices in Turkey. The research findings show that 1) Indonesian students still practice Islamic teachings while in Turkey, 2) Students use Islamic teachings and values as guidelines for carrying out daily activities and activities in Turkey, and 3) Students believe that religion is the basic motivation and guidance for life. strong and persistent in completing studies in Turkey.
Co-Authors Adam Hermawan Cita Ayni Putri Silalahi Dalmi Iskandar Sultani Dinil Abrar Edy Meiyanto Fathin Nazirah Aulia Suci Amanda Putri Sujarwo