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All Journal Journal of Biomedicine and Translational Research Media Medika Muda
Arlita Leniseptaria Antari
Dept Mikrobiologi Fakultas Kedokteran Universitas Diponegoro
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Macrophage Activity Test of Pulmonary Tuberculosis Patients with Diabetes Mellitus (TB-DM) Arlita Leniseptaria Antari; Indah Saraswati; David Pakaya; Aryoko Widodo
Journal of Biomedicine and Translational Research Vol 2, No 2 (2016): December 2016
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.528 KB) | DOI: 10.14710/jbtr.v2i2.833

Abstract

Background: Control of pulmonary TB is getting more and more complicated as the number of patients with diabetes mellitus (DM) is increasing. The increasing prevalence of DM is followed by the increasing prevalence of pulmonary TB. Diabetes Mellitus patients have 4,7 times higher risk to develop pulmonary TB compared to patients without DM, since DM can increase the frequency and severity of an infection, including pulmonary TB.Aim: To analyze macrophage activity (phagocytosis, intracellular killing, and TNF-α synthesis) of TB-DM patients.Method: The research is an experimental study using a PBMC cultured sample from TB-DM patient's which undergo observation of macrophage activity (phagocytic, intracellular killing and TNF-α synthesis). The data were taken from microscopic observation of TB-DM patients, colony growth of viable Mycobacterium tuberculosis and the TNF-α level secreted by macrophages.Result: Microscopic observation showed that there are less amount of phagocytosed M. tuberculosis (in macrophage/intracellular level) and there is a little amount of formed vacuoles and giant cells. Furthermore, macrophages in TB-DM patients secrete low level of TNF- α, and there are more viable M. tuberculosis from this macrophage.Conclusions: Macrophages of TB-DM patients are less activated, with reduced phagocytic activity (due to the intrinsic defect of PMN) and reduced antigen presenting activity of phagocytes toward M. tuberculosis. Therefore, there is a need for a further study focused on macrophage activity enhancement on TB-DM patients against M. tuberculosis infection in DM patients.Keywords: macrophage, TB-DM, phagocytosis, intracellular killing, TNF- α. 
The Effectivity of Parsley (Petroselinum crispum) Extract on The Growth Inhibition of Candida Albicans Rafinda Naditya Arismunandar; Isniya Nosartika; Brigitta Natania Renata Purnomo; Arlita Leniseptaria Antari
Journal of Biomedicine and Translational Research Vol 7, No 3 (2021): December 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i3.12555

Abstract

Background: Denture stomatitis is a type of Candida-associated infection that mainly affects the palatal mucosa. Candida albicans is one of the normal flora that is considered to be the primary etiologic agent in the pathogenesis of denture stomatitis. Denture decontamination is necessary to prevent denture stomatitis. One method of decontamination is by immersing removable dentures in an antifungal solution. Parsley (Petroselinum crispum) is a medicinal plant showing antifungal activity. Objectives: To determine the effectivity of immersion of acrylic resin Plate in parsley extract on inhibition of growth of Candida albicans. Methods: The effectivity of immersion of heat-cured acrylic resin plates in parsley extract on inhibition of C. albicans growth was tested using an experimental laboratory study with a post-test-only control group design. Thirty samples were divided into 5 groups as 0.01%, 0.02%, and 0.05% parsley ethanol extract, 0.1% sodium hypochlorite, and sterile aquadest.  The number of colony forming units per mL was obtained after calculating the colonies on SDA media, allowing the minimum inhibitory concentration (MIC) obtained according to the formula. Results: 0.01% parsley ethanol extract is MIC with a mean of 7.4 CFU / ml, which inhibited the growth of C. albicans by 31.05%. The Kruskal Wallis test (p<0.001) results indicate that there is an effectivity of immersion of acrylic resin plate in parsley extract on inhibition of growth of C. albicans. Conclusions: The immersion of acrylic resin plates in parsley ethanol extract with a concentration of 0.01%, 0.02%, and 0.04% has effectivity on the inhibition of growth of C. albicans.
UJI INTRACELLULAR KILLING TERHADAP MYCOBACTERIUM TUBERCULOSIS DARI MAKROFAG PENDERITA DAN INDIVIDU SEHAT BERISIKO TUBERKULOSIS PARU Arlita Leniseptaria Antari; David Pakaya; Dahliatul Qosimah
Media Medika Muda Vol 1, No 3 (2016)
Publisher : Faculty of Medicine Diponegoro University

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Abstract

Latar belakang: Makrofag merupakan sistem pertahanan yang pertama pada infeksi tuberkulosis, dimana masuknya Mycobacterium tuberculosis ke dalam makrofag dan kemampuan bertahan hidup didalamnya merupakan elemen kunci dari patogenesis tuberculosis. Penelitian ini bertujuan untuk menguji perbedaan kemampuan intracellular killing terhadap Mycobacterium tuberculosis antara makrofag penderita dan individu sehat berisiko tuberkulosis paru.Metode: PBMC diisolasi dari buffy coat penderita dan individu sehat berisiko tuberculosis paru. Monosit (105 cell/ml) dikultur dalam 24-wells tissue culture plate berisi coverslip, kemudian ditambahkan RPMI 1640 yang disuplementasi 10% HI–PHS (Heat Inactivated Pooled Human Serum) dan diinkubasi pada 37°C, 5 % CO2. Pada akhir periode inkubasi, kultur dipanen, dibilas PBS, Mycobacterium tuberculosis yang terbebas kemudian dikultur dalam media padat Middlebrook 7H10 dan diinkubasi selama 7 hari, 10 haridan 14 hari. Koloni Mycobacterium tuberculosis yang viabel dihitung sebagai CFU (Colony Forming Units).Hasil: Hasil penelitian menunjukkan bahwa jumlah koloni Mycobacterium tuberculosis yang viabel setelah intracellular killing lebih banyak terdapat pada makrofag penderita tuberkulosis paru daripada makrofag individu sehat berisiko tuberkulosis paru. Hasil analisa dengan menggunakan uji Univariate Analysis of Variance menunjukkan adanya perbedaan yang signifikan (p < 0,01).Simpulan: Intracellular killing makrofag penderita tuberkulosis paru lebih rendah daripada individu sehat berisiko tuberkulosis paru. Hal tersebut ditunjukkan dengan lebih banyaknya jumlah koloni Mycobacterium tuberculosis yang viabel pada media Middlebrook 7H10 yang berasal dari makrofag penderita tuberkulosis paru. Kata kunci: Mycobacterium tuberculosis, makrofag, intracellular killing.
Co-Authors Aryoko Widodo Brigitta Natania Renata Purnomo David Pakaya Indah Saraswati Nosartika, Isniya Qosimah, Dahliatul Rafinda Naditya Arismunandar