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All Journal Plantropica: Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science
Darmawan Saptadi
Brawijaya University
Agriculture, Biological Sciences & Forestry

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Tahap Awal Seleksi Galur Murni Ercis (Pisum sativum L.) Populasi Lokal Boyolali dan Temanggung Berdasarkan Karakteristik Fisik Biji Fildza Abidah; Darmawan Saptadi; Budi Waluyo
PLANTROPICA: Journal of Agricultural Science Vol 6, No 1 (2021)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpt.2020.006.1.10

Abstract

Pea (Pisum sativum L.) is a nutritious and high-value vegetable. Plant cultivation and the selection of superior cultivars are two ways to increase pea production. One technique of plant breeding for improved varieties is to get some genotipes from local populations. The genetic potential of the pure line selection based on the physical features of the seeds is indicated agronomic and morphological variability. The purpose of this study is to investigate agronomic characteristics and heritability of pea lines developed based on physical seed selection in local population. This research used randomized block desig) consist 22 lines based on seed characteristics selection, which are 12 local lines from Boyolali and 10 local lines from Temanggung as a treatment and repeated three times. Research has been carried out on January 2019 to April 2019 and located at Abdulrachman Saleh Air Force Base Agricultural Experimental Green House, Malang. Agronomics characters that have medium variability are flowering day, length from stem to first pod, length of leaf, length between first and second pods, length of tendrils, length of stem, number of branches, number of bracts, number of seeds per plant,  weight of dry pods, weight of dry seeds per pod, length of dry seeds, width of dry seeds, and weight of dry seeds per plant. The high heritability are found inflowering day, day to harvesting dry pods, and length of nodes.
Genetic Diversity of Indonesian Physic Nut (J. curcas) Based on Molecular Marker Darmawan Saptadi; Rr. Sri Hartati; Asep Setiawan; Bambang Heliyanto; Sudarsono Sudarsono
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.694

Abstract

Various reports of molecular genetic diversity evaluation of physic nut (J. curcas) have given inconsistent results. Part of the reasons were because of the used of unrealiable markers. This study was conducted to evaluate genetic diversity of Indonesian physic nut germplasm using four types of molecular markers (RAPD, ISSR, SSR and SCAR markers). Twenty four J. curcas accessions planted in Pakuwon, Sukabumi, with various phenotypes were evaluated. Twenty eight SSR marker loci yielded monomorphic allele pattern and indicated that the evaluated accessions probably were all genetically homogeneous for the respective loci. Eight RAPD and 4 ISSR primers out of the total 31 tested primers produced scoreable markers and some (i.e. UBC 873, OPG 17, OPP 03 and OPQ 11 primers) generated polymorphics markers. Genetic similarity coefficiens among evaluated accessions ranged from 0.6 to 1.0 with a population mean of 0.9 indicating low diversity and narrow genetic background among accessions in all populations. Therefore, breeding program utilizing such population would only result in low genetic gain. Based on the evaluated SCAR markers, all accessions belonged to the non-toxic Mexican type of physic nut. This information is important inputs for designing future physic nut breeding strategies in Indonesia.
Co-Authors Bambang Heliyanto Budi Waluyo Fildza Abidah Rr. Sri Hartati Setiawan, Asep S Sudarsono Sudarsono