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Sudarsono Sudarsono
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry

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DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica) Meynarti Sari Dewi Ibrahim; Rr. Sri Hartati; Rubiyo Rubiyo; Agus Purwito; Sudarsono Sudarsono
Indonesian Journal of Agricultural Science Vol 14, No 2 (2013): October 2013
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v14n2.2013.p79-86

Abstract

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.
Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR Fetrina Oktavia; Kuswanhadi Kuswanhadi; Dini Dinarty; Widodo Widodo; Sudarsono Sudarsono
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.881

Abstract

The accession of the IRDB 1981 (PN’81) population is a newly introduced and an important rubber tree germplasm while the Wickham clone is a commercial variety one. The objectives of this study were to assess the genetic diversity and the population structure of PN’81 populations and the Wickham clones using 15 EST-SSR loci. Results of the analysis showed that the evaluated SSR primers yielded polymorphic markers. The gSSR 268 primer pairs yielded the most informative markers while HBE 280 primers generated the lowest ones. Results of the genetic diversity analysis supported that the PN’81 population belonged to a single large natural population of rubber trees while the Wickham clones belonged to a different group than that of PN’81. The population structure analysis of the rubber accessions was also in agreement with the results of the genetic diversity analysis. The experiment also indicated that PN’81 populations would be useful for future rubber breeding in Indonesia, especially as the sources of parent clones for rubber tree hybridization programs and rubber tree genetic resource conservation.
Genetic Diversity of Indonesian Physic Nut (J. curcas) Based on Molecular Marker Darmawan Saptadi; Rr. Sri Hartati; Asep Setiawan; Bambang Heliyanto; Sudarsono Sudarsono
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.694

Abstract

Various reports of molecular genetic diversity evaluation of physic nut (J. curcas) have given inconsistent results. Part of the reasons were because of the used of unrealiable markers. This study was conducted to evaluate genetic diversity of Indonesian physic nut germplasm using four types of molecular markers (RAPD, ISSR, SSR and SCAR markers). Twenty four J. curcas accessions planted in Pakuwon, Sukabumi, with various phenotypes were evaluated. Twenty eight SSR marker loci yielded monomorphic allele pattern and indicated that the evaluated accessions probably were all genetically homogeneous for the respective loci. Eight RAPD and 4 ISSR primers out of the total 31 tested primers produced scoreable markers and some (i.e. UBC 873, OPG 17, OPP 03 and OPQ 11 primers) generated polymorphics markers. Genetic similarity coefficiens among evaluated accessions ranged from 0.6 to 1.0 with a population mean of 0.9 indicating low diversity and narrow genetic background among accessions in all populations. Therefore, breeding program utilizing such population would only result in low genetic gain. Based on the evaluated SCAR markers, all accessions belonged to the non-toxic Mexican type of physic nut. This information is important inputs for designing future physic nut breeding strategies in Indonesia.
Co-Authors Agus Purwito Bambang Heliyanto Darmawan Saptadi Dini Dinarty Fetrina Oktavia Iskandar Iskandar Kuswanhadi Kuswanhadi Meynarti Sari Dewi Ibrahim Nurmegawati Nurmegawati Rr. Sri Hartati Rubiyo Rubiyo Setiawan, Asep S Widodo Widodo