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The Use of Electrophoretic Isozymes to Detect Tungro Infected Rice Suranto Suranto; Arief A.; Supyani Supyani
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.643

Abstract

Tungro is one of the most common diseases of rice plants which caused by double infection of RTBV (Rice Tungro Bacilliform Virus) and RTSV (Rice Tungro Spherical Virus), and it gives a significant economic loss. These viruses are transmitted by green leaf hopper (Nephottetix virescens. Distant), and the interaction between host plant and the viruses were still quite difficult to be fully understood. In order to look at whether there are any differences between the healthy and tungro infected rice, this study was set to examine the character differences between the infected and the healthy plants based on isozyme banding pattern. The infected plants were collected from three districts around Surakarta (Sragen, Sukoharjo, Klaten) followed by Polyacrilamide Gel Electrophoresis (PAGE) to evaluate the isozyme banding patterns. There were peroxidase, esterase and acid phosphotase isozymes used. The results showed that the real differences of isozyme banding patterns of both healthy and tungro infected plants were discovered. In all cases, the Tungro infected rice had thicker and more band numbers compared to the healthy one. This evident suggested that Tungro infected rice could be detected early using PAGE method.
APLIKASI DIAGNOSA KERUSAKAN MESIN SEPEDA MOTOR BEBEK 4 TAK DENGAN METODE FORWARD CHAINING Supyani Supyani; Bebas Widada; Wawan Laksito Yuly Saptomo
Jurnal Teknologi Informasi dan Komunikasi (TIKomSiN) Vol 1, No 1 (2013): Jurnal TIKomSiN
Publisher : STMIK Sinar Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.041 KB) | DOI: 10.30646/tikomsin.v1i1.78

Abstract

Sistem pakar adalah program komputer yang berfungsi sebagai konsultan ahli untuk suatu bidang tertentu. Pemakai yang menggunakan program ini seolah-olah berhadapan langsung dengan pakar yang sebenarnya. Perencanaan sistem dalam membuat knowledge base memakai  Aturan if-then sebagai representasi pengetahuan. Pembuatan metode inferensi memakai metode forward chaining yang telah dimodifikasi sehingga sesuai dengan permasalahan.Implementasi program sistem pakar ini mengunakan bahasa pemograman PHP. Aplikasi ini akan menghasilkan jenis-jenis kerusakan yang terjadi pada sepeda motor serta solusi penanganan dari kerusakan tersebut. Adapun metode pengumpulan data yang dilakukan oleh penulis adalah metode wawancara, metode observasi dan metode studi pustaka. Tujuan dari penelitian ini adalah membantu menganalisa kerusakan mesin sepeda motor bagi mekanik pemula dan para siswa yang sedang prakerin, hasilnya tercipta sebuah aplikasi yang dapat mendiagnosa kerusakan mesin sepeda motor untuk membantu siswa yang sedang melaksanakan prakerin dalam mendiagnosa kerusakan mesin.Kata kunci : metode forward chaining, php, sistem pakar
Hubungan Karakteristik Biologis Gambut dengan Penyakit Busuk Batang yang Disebabkan Ganoderma pada Kelapa Sawit Supriyanto Supriyanto; Purwanto Purwanto; S H Poromarto; Supyani Supyani
Perkebunan dan Lahan Tropika Vol 11, No 1 (2021)
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/plt.v11i1.53357

Abstract

The relationship of biological properties of peat with oil palm basal stem rot (BSR) caused by Ganoderma.  Indonesia as the largest palm oil producer in the world, has the large percentage of oil palm on peatland. The serious problems of cultivating oil palm on peatland is the presence of BSR disease caused by Ganoderma. There are no effective methods to control the spread of this disease. Biological control is an environmentally friendly alternative method focus, but on peatland, the development of this method is constrained by lack of information about the effect of biological environmental factors. This study aims to determine the effect of the biological propertiess  of peat on the intensity of Ganoderma attacks on oil palm. The relationship between the intensity of Ganoderma attacks and biological properties of peat was carried out by correlation test. The correlation analysis showed that the biological properties  of peat has not correlated with the Ganoderma attacks, except Summed Dominant Ratio of vegetation which tends to positively correlate to the number of Ganoderma antagonists. These results indicate that peatland vegetation management by maintaining the diversity of vegetation around oil palm can help reduce Ganoderma attacks.