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All Journal BERKALA SAINSTEK Journal of Mathematical and Fundamental Sciences Metamorfosa: Journal of Biological Science
Sutoyo Sutoyo
Department Of Biology, Faculty Mathematics And Natural Sciences, University Of Jember
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Mathematics Physics

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Phosphate Solubilizing Bacteria Adaptive to Vinasse Kahar Muzakhar; Sutoyo Sutoyo; Ahmad Bukhari Saragih
Journal of Mathematical and Fundamental Sciences Vol. 47 No. 2 (2015)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2015.47.2.8

Abstract

Microorganisms identified as phosphate solubilizing bacteria (PSB) adaptive to vinasse were successfully screened from sugarcane soil from an agriculatural estate in Jatiroto. By conducting a screening on Pikovskaya's agar medium (PAM), we found that five different isolates were detected as PSB (pvk-5a, pvk-5b, pvk-6b, pvk-7a, and pvk-8a). Of the five isolates only three could be grown and were found to be adaptive to vinasse based medium without any nutrients added (pvk-5a, pvk-5b and pvk-7a). The three isolates were  characterized as coccus and Gram negative with no endospores detected. We suggest that these three isolates can be used as biofertilizer agent to  support organic farming.
Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.) Azhari, Fitri; Winarsa, Rudju; Siswanto, Siswanto; Muzakhar, Kahar; Utarti, Esti; Sutoyo, Sutoyo; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.19034

Abstract

Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies.
AKTIVITAS PROTEOLITIK DAN FIBRINOLITIK ISOLAT BAKTERI DARI PERAIRAN PANTAI PAPUMA KABUPATEN JEMBER Setiawan, Arif; Arimurti, Sattya; Senjarini, Kartika; Sutoyo, Sutoyo
BERKALA SAINSTEK Vol 4 No 1 (2016)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v4i1.4457

Abstract

Berbagai jenis bakteri dari perairan pantai dapat dieksplorasi sebagai sumber penghasil senyawa penting. Sejauh ini, bakteri dari Perairan Pantai Papuma Kabupaten Jember yang memiliki aktivitas proteolitik dan fibrinolitik belum dilaporkan. Tujuan penelitian ini adalah menguji aktivitas proteolitik dan fibrinolitik pada sejumlah isolat bakteri. Metode penelitian meliputi uji aktivitas proteolitik menggunakan Media Skim Milk Agar (SMA) terhadap 23 isolat bakteri dan uji aktivitas fibrinolitik menggunakan Media Fibrin pada isolat yang diketahui memiliki aktivitas proteolitik. Metode uji aktivitas dilakukan secara semikuantitatif. Sebanyak 11 isolat bakteri memiliki aktivitas proteolitik. Isolat bakteri WU 021012* memiliki indeks proteolitik tertinggi yaitu 4,3. Isolat bakteri yang fibrinolitik diperoleh sebanyak 3 isolat. Isolat bakteri WU 021055* memiliki indeks tertinggi sebesar 11.
Kapang amilolitik asal Biosite Hutan Pelangi, Ijen Geopark, Indonesia Sutoyo Sutoyo; Safira Isti’nafil Islam; Esti Utarti; Sattya Arimurti; Siswanto Siswanto
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p10

Abstract

Vegetasi Hutan Pelangi, Ijen Geopark sudah terkonservasi sejak lama, sehingga kemungkinan di dalamnya terdapat diversitas mikrobiota yang potensial sebagai agen hayati pendegradasi berbagai substrat organik. Keberadaan dan pertumbuhan mikroba dalam lingkungan Hutan Pelangi berperan penting dalam membentuk proses antara lain siklus karbon, sehingga keberadaan mikroorganisme tersebut menarik untuk dikaji secara mikrobiologi. Penelitian ini bertujuan untuk mendapatkan isolat yang mampu mendegradasi substrat amilum secara semikuantitatif dan identifikasi secara morfologi sampai tingkat genus terhadap isolat terpilih. Penelitian diawali dengan isolasi, skrining kapang amilolitik serta identifikasinya secara fenotip terhadap isolat yang potensial aktivitas degradasinya tinggi. Hasil penelitian diperoleh 34 isolat kapang yang menunjukkan keragaman berbeda secara morfologi koloninya dan aktivitasnya dalam menghidrolisis amilum. Sejumlah 22 isolat (64,7%) menunjukkan aktivitas amilolitik dan 12 isolat (35,29%) merupakan kapang non-amilolitik. Hasil ANOVA rata-rata indeks aktivitas amilolitik isolat kapang dan uji lanjut Duncan menunjukkan bahwa isolat kapang IHP30, IHP10, dan IHP9 merupakan tiga isolat kapang amilolitik dengan indeks aktivitas amilolitik tertinggi. Indeks aktivitas masing-masing yaitu isolat kapang IHP30 sebesar 1,65 ± 0,14; IHP10 sebesar 1.47 ± 0,31; dan IHP9 sebesar 1.17 ± 0,06. Berdasarkan karakteristiknya, masing-masing teridentifikasi sebagai Penicillium sp. IHP30, Penicillium sp. IHP10, dan Aspergillus sp. IHP9
Co-Authors Ahmad Bukhari Saragih Arif Setiawan Azhari, Fitri Dwi Setyati Esti Utarti Kahar Muzakhar Kartika Senjarini S Siswanto Safira Isti’nafil Islam Saniyah Fatkhul Alim Sattya Arimurti Siswanto Siswanto Winarsa, Rudju