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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication LENSA (Lentera Sains): Jurnal Pendidikan IPA Indonesian Chimica Letters Life Science and Biotechnology

Esti Utarti

Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember

Author-ID : 2976204

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Education Environmental Science Health Professions Immunology & microbiology Mathematics Nursing Physics Public Health

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TRANSFORMASI GEN SOSUT1 PADA TANAMAN TEBU MENGGUNAKAN AGROBACTERIUM TUMEFACIENS STRAIN GV 3101 DAN PANGKAL TUNAS TEBU IN VITRO Edia, Edia F.D; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

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Gen SoSUT1 merupakan gen pengkode protein sucrose transporter yang memfasilitasi proses transpotasi sukrosa dari jaringan fotosintetik (source) ke jaringan pengguna (sink) pada tanaman tebu. Tujuan penelitian ini adalah mendapatkan tanaman tebu transforman melalui transformasi genetik menggunakan vektor Agrobacterium tumefaciens yang membawa gen SoSUT1. Eksplan pangkal tunas tebu in vitro diinfeksi dengan A. tumefaciens yang membawa konstruk pAct-SoSUT1 dan dilakukan seleksi pada media MS dengan penambahan antibiotik hygromycin.. Tanaman putatif transforman yang telah berhasil melewati proses seleksi dan diaklimatisasi, dilakukan isolasi DNA genom kemudian dianalisis PCR. Hasil analisis PCR dengan pasangan primer 1F/1R hpt II menunjukkan bahwa dari 24 tanaman tebu putatif transforman, didapatkan 15 tanaman tebu positif mengandung gen SoSUT1. Efektifitas rata-rata transformasi gen SoSUT1 menggunakan eksplan pangkal tunas tebu in vitro sebesar 6,8%. Kata Kunci: Agrobacterium tumefaciens, SoSUT1, sukrosa.

The Isolasi Aktinomiset Pelarut Fosfat Asal Perakaran Tembakau (Nicotiana tabacum L.) di Antirogo Jember Esti Utarti; Saniyah Fatkhul Alim; Dwi Setyati; S Sutoyo
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p09

Fosfor (P) merupakan makronutrien terpenting kedua yang dibutuhkan tanaman. Fosfat dalam tanah berada dalam bentuk terikat oleh ion Fe3+, Al3+ dan Ca2+ sehingga tidak tersedia bagi tanaman. Penelitian ini bertujuan untuk mengisolasi dan mendapatkan aktinomiset sebagai agen pelarut fosfat dari perakaran tembakau (Nicotiana tabacum L.). Jenis penelitian ini termasuk dalam penelitian eksplorasi yang dilanjutkan dengan pengamatan morfologi secara makroskopis dan mikroskopis isolat pelarut fosfat terbaik. Sampel penelitian diambil dari Desa Antirogo, Kecamatan Sumbersari, Kabupaten Jember. Sampel tanah diambil dari bagian perakaran tanaman tembakau. Isolasi aktinomiset dilakukan dengan metode pengenceran dan disebar pada media SCA. Pemurnian dilakukan pada media ISP-2 atau ISP-4. Uji aktivitas pelarutan fosfat menggunakan media Pikovskaya dan diukur indeks pelarutan fosfatnya. Sebanyak 71 isolat aktinomiset berhasil diisolasi dari tanah perakaran tembakau di daerah Antirogo. Hasil skrining menunjukkan bahwa terdapat 28 isolat (39,4%) dari 71 isolat yang diperoleh, memiliki kemampuan melarutkan fosfat. Lima isolat tertinggi berdasarkan rangking indeks pelarut fosfat terbaik dilakukan karakterisasi morfologi makroskopis dan mikroskopis. Berdasarkan bentuk rantai spora, empat isolat aktinomiset yang memiliki rangking indeks pelarutan fosfat tertinggi termasuk dalam genus Streptomyces. Kata kunci: aktinomiset, pelarut fosfat, perakaran, Antirogo Jember

SCREENING AND IDENTIFICATION ALKALY-CELLULOLYTIC MOLDS FROM RICE STRAW ON COASTAL-FIELD OF WATU ULO JEMBER Esti Utarti; Su’udah Hasanah; Siswanto Siswanto
Jurnal ILMU DASAR Vol 13 No 1 (2012)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

About 28 molds were obtained from rice straw on coastal-field of Watu Ulo Jember, were screened for their cellulolytic activities at pH alkaline. In semiquantitatively screening on CMC and Avicel plate at pH 8 showed that 27 isolates have cellulolytic activities. Based on clearing zone in CMC plate, isolates 7, 9, 14, 19 and 24 have higher cellulase (CMC-ase) activities index at pH alkaline. Further, in quantitatively examination using rice straw in Basic Salt Mandel’s modification medium showed isolate 19 (0,60 U/ml) that identified as Aspergillus terreus have higher FP-ase activity than isolate 7 (0,56 U/ml), 9 (0,55 U/ml), 14 (0,53 U/ml) and 24 (0,56 U/ml).

Characterization of Crude Protease Bacillus sp 31 Esti Utarti; Lina Nurita; Sattya Arimurti
Jurnal ILMU DASAR Vol 10 No 1 (2009)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.094 KB)

Bacillus sp 31 was bacteria which produce protease. Characterization of protease from Bacillus sp 31 i.e. pH, temperature, influence of metal ion, enzyme kinetic and enzyme termostability is important to get optimal enzyme activity. Protease activity showed values 146.40 U/ml on pH 9 and optimal temperature 60°C by value. Protease activity increased by addition of 159.50 U/ml Fe2+, but its activity decreased by addition of Mg2+, Cu2+, Ca2+, Al2+, Zn2+ dan Mn2+. Maximal velocity (Vmax) of enzyme-catalysed reactions was 21.32 U/ml with Km 1.5x10-3 mg/ml (Michaels-Menten Kinetic). Protease was very stable at 60°C for 4 hours of incubation and 7 hours of half-time.

Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi Evi Umayah Ulfa; Esti Utarti; Izzay Afkarina; Sattya Arimurti; Kartika Senjarini
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Universitas Islam Bandung

Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.

KARAKTERSITIK KHAMIR AMILOLITIK DARI BERBAGAI MACAM BUAH Jefri Nur Hidayat; Siswanto Siswanto; Esti Utarti
LENSA (Lentera Sains): Jurnal Pendidikan IPA Vol. 2 No. 2 (2012): November 2012
Publisher : Faculty of Teaching and Education, University of Wiraraja

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24929/lensa.v2i2.150

Enam puluh juta ton amilum per tahun di Indonesia memiliki nilai jual yang rendah berdasarkan nilai ekonomi skala internasional. Peningkatan pemanfaatan amilum dapat dilakukan dengan menghidrolisis amilum menjadi glukosa secara enzimatik oleh mikrob amilolitik. Yeast amilolitik memiliki pertumbuhan yang lebih cepat, lebih efektif memecah amilum dari kapang dan dapat digunakan sebagai protein sel tunggal. Penelitian ini bertujuan untuk mendapatkan isolat yeast amilolitik yang berasal dari buah sukun, buah pisang, buah mangga dan buah durian. Hasil isolasi didapatkan 11 isolat yeast. Uji semikuantitatif dengan media SSA terhadap 11 isolat yeast, terdapat 5 isolat yang bersifat amilolitik yaitu isolat SiJi-P3, SiJi-P5, SiJi-S1, SiJi-S2, dan SiJi-M2. Isolat SiJi-P3 dan SiJi-P5 yang teridentifikasi sebagai Saccarhomyceteae mempunyai indek amilolitik terbesar.

Skrining Aktinomisetes Pendegradasi Nikotin Pada Daun Tembakau (Nicotiana tabacum L.) Esti Utarti; Dina Amalia Syahidah; Sattya Arimurti
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p14

Kabupaten Jember merupakan salah satu sentral penghasil tembakau tertinggi di Jawa Timur dan pada tahun 2018 produksinya mencapai 163,267,5 ton. Produksi tanaman tembakau menghasilkan limbah organik yang berbahaya yaitu nikotin. Nikotin merupakan alkaloid beracun aktif, berminyak, tersusun atas unsur karbon, hidrogen, nitrogen, dan sangat larut terhadap alkohol, eter, minyak tanah, dan air. Kelarutannya berisiko mengalami leaching (pencucian) selama masa penyimpanan limbah tembakau. Hal tersebut mengakibatkan nikotin yang tercuci dapat mengalir ke dalam badan air sehingga membahayakan makhluk hidup dan lingkungan. Beberapa mikroorganisme mampu mendegradasi nikotin dengan memanfaatkannya sebagai sumber karbon dan nitrogen untuk pertumbuhannya, salah satunya aktinomisetes. Penelitian ini bertujuan untuk mendapatkan aktinomisetes asal rhizosfer tembakau yang memiliki aktivitas pendegradasi nikotin dan besar daya degradasinya. Penelitian dilakukan skrining 23 isolat aktinomisetes pendegradasi nikotin dengan indikator tumbuh aktinomisetes pada media hingga terdapat 3 isolat terpilih, uji degradasi nikotin pada media cair dengan metode titrasi dan daun tembakau dilakukan di Balai Pengujian dan Sertifikasi Mutu Barang (BPSMB) kota Surakarta, dan identifikasi morfologi secara mikroskopis isolat terpilih menggunakan cover slide method. Hasil skrining menunjukkan bahwa 5 isolat memiliki kemampuan tumbuh, 13 isolat mumpu tumbuh baik, 3 isolat tumbuh lebih baik dan 2 isolat tidak tumbuh. Isolat yang terpilih yaitu ATG 60, ATG 68, dan ATG 69 tumbuh lebih baik pada media nikotin (NIM). Daya degradasi nikotin media cair dan daun tembakau pada ATG 60 (2,33% dan 30,56%); ATG 68 (2,53% dan 31,16%); dan ATG 69 (2,1% dan 25,82%). Identifikasi struktur spora menunjukkan masing-masing isolat adalah Saccharopolyspora sedangkan ATG 68 dan 69 genus Streptomyces.

Isolation and potency of Actinomycetes from rhizosphere of nutmeg (Myristica fragrans Houtt) Ferymon Mahulette; Esti Utarti; Tri Santi Kurnia
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.35632

Nutmeg (Myristica fragrans Houtt) is commonly cultivated by people in the forests of Moluccas Islands. This plant grows well on relatively infertile soil types. This is presumably due to the presence of symbiotic microbes in the root of nutmeg. The research aimed to isolate, characterize and test the potential of Actinomycetes from rhizosphere of nutmeg. Soil sample were taken from the nutmeg forest in Ambon Island. The Actinomycetes isolation using humic acid vitamin, continued with yeast malt agar (YMA) media. The testing of antibacterial and antifungal activities using YMA media, while cellulolytic activity, phosphate solubilizing, and xylanolytic activity using carboxyl methyl cellulose, Picovskaya agar, and birchwood agar or oat spelt xylan agar. A total of 12 isolates of Actinomycetes were isolated and dominated by Streptomyces with various types of aerial mycelia. The substrate mycelium looks brown and cream, while the aerial mycelium looks white and gray. These isolates had the highest inhibitory power against Escherichia coli and Fusarium oxysporum with indexes of 16.5 mm and 16.0 mm, respectively. The other isolates have the ability of cellulolytic, phosphate solubilizing, and xylanolytic with indexes 3.26, 3.87, and 1.2, respectively. The Actinomycetes isolates that were found can be used as starter to improve the biofertilizer formula for nutmeg.

The HEMOLYSIS ACTIVITY OF BACTERIA ISOLATS FROM PELANGI FOREST OF IJEN GEOPARK Sattya Arimurti; esti Utarti; sutoyo sutoyo; siswanto siswanto; Tantri Raras Ayuningtyas
Life Science and Biotechnology Vol 1 No 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44097

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code IHP. These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). Safety evaluations based on the hemolysis reactions offer simple tests to ease the analysis of potential pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest for their hemolysis reactions. The hemolysis test was conducted using blood agar media, from which isolates with a negative (λ) reaction. Based on the results of hemolysis tests, 30 out of 153 bacterial isolates (19.60%) were found to be negative reactions. These bacteria are safe to proceed with for potential analysis.

Optimization of Centrifugation Speed and pH in Extraction of Uricase Enzyme from Goat Liver Handayani, Wuryanti; Esti Utarti; Riki Juni Krismiadi; AA. Istri Ratnadewi
Indonesian Chimica Letters Vol. 1 No. 2 (2022)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

The human body does not have an enzyme that can break down uric acid, so the accumulation of uric acid can cause disease. This problem can be overcome by uricolytic therapy by utilizing the activity of the uricase enzyme. In this study, the uricase enzyme was extracted from goat liver by optimizing the centrifugation speed and the extraction pH. The purpose of the optimization is to get maximum uricase activity. Uricase extraction to optimize centrifugation speed using borate buffer pH 8.5 then centrifuged at 7.000; 9.000; 11.000; 13.000 and 15.000 rpm with a temperature of 4oC. Furthermore, pH optimization was carried out using pH buffers 6, 7, 8, 9, 10 and 11 by centrifuging the optimum speed obtained. The crude extract obtained was further tested for its enzyme activity . The results showed that the highest uricase activity was achieved if the extraction was carried out at pH 8 using centrifugation at an optimum speed of 13,000 rpm. The higher uricase activity indicates that the extracted uricase concentration is increasing.

Co-Authors AA. Istri Ratnadewi Anggitasari, Dhanti Fatma Anja Meryandini Antonius Suwanto Azhari, Fitri Bambang Sugiharto Dina Amalia Syahidah Dwi Setyati Edia F.D Edia, Edia F.D Eva Tyas Utami Evi Umayah Ulfa Farrennina, Tasya Preira Ferymon Mahulette Ida Ayu Putu Sri Widnyani Izzay Afkarina Jefri Nur Hidayat Kahar Muzakhar Kartika Senjarini Lina Nurita Maggy T Suhartono Media Ningtyas, Rinda Nashrullah, Shafa Purniasari, Fina Yunita Putri, Amelia Fahreza Riki Juni Krismiadi S Siswanto S Sutoyo S. Siswanto Saniyah Fatkhul Alim Sattya Arimurti Sattya Arimurti Siswanto Siswanto Siswanto Siswanto Suksma, Nadhea Ayu Sutoyo Sutoyo Sutoyo Sutoyo Su’udah Hasanah Tantri Raras Ayuningtyas Tri Santi Kurnia Winarsa, Rudju Wuryanti Handayani

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