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All Journal Jurnal Ilmu Dasar BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication Jurnal Biodjati LENSA (Lentera Sains): Jurnal Pendidikan IPA Abdimasku : Jurnal Pengabdian Masyarakat JPM: JURNAL PENGABDIAN MASYARAKAT Indonesian Chimica Letters Life Science and Biotechnology Metamorfosa: Journal of Biological Science Open Access DRIVERset

Esti Utarti

Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember

Author-ID : 2976204

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Transportation Other

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TRANSFORMASI GEN SOSUT1 PADA TANAMAN TEBU MENGGUNAKAN AGROBACTERIUM TUMEFACIENS STRAIN GV 3101 DAN PANGKAL TUNAS TEBU IN VITRO Edia, Edia F.D; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

Show Abstract | Download Original | Original Source | Check in Google Scholar

Gen SoSUT1 merupakan gen pengkode protein sucrose transporter yang memfasilitasi proses transpotasi sukrosa dari jaringan fotosintetik (source) ke jaringan pengguna (sink) pada tanaman tebu. Tujuan penelitian ini adalah mendapatkan tanaman tebu transforman melalui transformasi genetik menggunakan vektor Agrobacterium tumefaciens yang membawa gen SoSUT1. Eksplan pangkal tunas tebu in vitro diinfeksi dengan A. tumefaciens yang membawa konstruk pAct-SoSUT1 dan dilakukan seleksi pada media MS dengan penambahan antibiotik hygromycin.. Tanaman putatif transforman yang telah berhasil melewati proses seleksi dan diaklimatisasi, dilakukan isolasi DNA genom kemudian dianalisis PCR. Hasil analisis PCR dengan pasangan primer 1F/1R hpt II menunjukkan bahwa dari 24 tanaman tebu putatif transforman, didapatkan 15 tanaman tebu positif mengandung gen SoSUT1. Efektifitas rata-rata transformasi gen SoSUT1 menggunakan eksplan pangkal tunas tebu in vitro sebesar 6,8%. Kata Kunci: Agrobacterium tumefaciens, SoSUT1, sukrosa.

SCREENING AND IDENTIFICATION ALKALY-CELLULOLYTIC MOLDS FROM RICE STRAW ON COASTAL-FIELD OF WATU ULO JEMBER Esti Utarti; Su’udah Hasanah; Siswanto Siswanto
Jurnal ILMU DASAR Vol 13 No 1 (2012)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

About 28 molds were obtained from rice straw on coastal-field of Watu Ulo Jember, were screened for their cellulolytic activities at pH alkaline. In semiquantitatively screening on CMC and Avicel plate at pH 8 showed that 27 isolates have cellulolytic activities. Based on clearing zone in CMC plate, isolates 7, 9, 14, 19 and 24 have higher cellulase (CMC-ase) activities index at pH alkaline. Further, in quantitatively examination using rice straw in Basic Salt Mandel’s modification medium showed isolate 19 (0,60 U/ml) that identified as Aspergillus terreus have higher FP-ase activity than isolate 7 (0,56 U/ml), 9 (0,55 U/ml), 14 (0,53 U/ml) and 24 (0,56 U/ml).

Characterization of Crude Protease Bacillus sp 31 Esti Utarti; Lina Nurita; Sattya Arimurti
Jurnal ILMU DASAR Vol 10 No 1 (2009)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.094 KB)

Bacillus sp 31 was bacteria which produce protease. Characterization of protease from Bacillus sp 31 i.e. pH, temperature, influence of metal ion, enzyme kinetic and enzyme termostability is important to get optimal enzyme activity. Protease activity showed values 146.40 U/ml on pH 9 and optimal temperature 60°C by value. Protease activity increased by addition of 159.50 U/ml Fe2+, but its activity decreased by addition of Mg2+, Cu2+, Ca2+, Al2+, Zn2+ dan Mn2+. Maximal velocity (Vmax) of enzyme-catalysed reactions was 21.32 U/ml with Km 1.5x10-3 mg/ml (Michaels-Menten Kinetic). Protease was very stable at 60°C for 4 hours of incubation and 7 hours of half-time.

Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi Evi Umayah Ulfa; Esti Utarti; Izzay Afkarina; Sattya Arimurti; Kartika Senjarini
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Universitas Islam Bandung

Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.

KARAKTERSITIK KHAMIR AMILOLITIK DARI BERBAGAI MACAM BUAH Jefri Nur Hidayat; Siswanto Siswanto; Esti Utarti
LENSA (Lentera Sains): Jurnal Pendidikan IPA Vol. 2 No. 2 (2012): November 2012
Publisher : Faculty of Teaching and Education, University of Wiraraja

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24929/lensa.v2i2.150

Enam puluh juta ton amilum per tahun di Indonesia memiliki nilai jual yang rendah berdasarkan nilai ekonomi skala internasional. Peningkatan pemanfaatan amilum dapat dilakukan dengan menghidrolisis amilum menjadi glukosa secara enzimatik oleh mikrob amilolitik. Yeast amilolitik memiliki pertumbuhan yang lebih cepat, lebih efektif memecah amilum dari kapang dan dapat digunakan sebagai protein sel tunggal. Penelitian ini bertujuan untuk mendapatkan isolat yeast amilolitik yang berasal dari buah sukun, buah pisang, buah mangga dan buah durian. Hasil isolasi didapatkan 11 isolat yeast. Uji semikuantitatif dengan media SSA terhadap 11 isolat yeast, terdapat 5 isolat yang bersifat amilolitik yaitu isolat SiJi-P3, SiJi-P5, SiJi-S1, SiJi-S2, dan SiJi-M2. Isolat SiJi-P3 dan SiJi-P5 yang teridentifikasi sebagai Saccarhomyceteae mempunyai indek amilolitik terbesar.

Isolation and potency of Actinomycetes from rhizosphere of nutmeg (Myristica fragrans Houtt) Ferymon Mahulette; Esti Utarti; Tri Santi Kurnia
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.35632

Nutmeg (Myristica fragrans Houtt) is commonly cultivated by people in the forests of Moluccas Islands. This plant grows well on relatively infertile soil types. This is presumably due to the presence of symbiotic microbes in the root of nutmeg. The research aimed to isolate, characterize and test the potential of Actinomycetes from rhizosphere of nutmeg. Soil sample were taken from the nutmeg forest in Ambon Island. The Actinomycetes isolation using humic acid vitamin, continued with yeast malt agar (YMA) media. The testing of antibacterial and antifungal activities using YMA media, while cellulolytic activity, phosphate solubilizing, and xylanolytic activity using carboxyl methyl cellulose, Picovskaya agar, and birchwood agar or oat spelt xylan agar. A total of 12 isolates of Actinomycetes were isolated and dominated by Streptomyces with various types of aerial mycelia. The substrate mycelium looks brown and cream, while the aerial mycelium looks white and gray. These isolates had the highest inhibitory power against Escherichia coli and Fusarium oxysporum with indexes of 16.5 mm and 16.0 mm, respectively. The other isolates have the ability of cellulolytic, phosphate solubilizing, and xylanolytic with indexes 3.26, 3.87, and 1.2, respectively. The Actinomycetes isolates that were found can be used as starter to improve the biofertilizer formula for nutmeg.

Optimization of Centrifugation Speed and pH in Extraction of Uricase Enzyme from Goat Liver Handayani, Wuryanti; Esti Utarti; Riki Juni Krismiadi; AA. Istri Ratnadewi
Indonesian Chimica Letters Vol. 1 No. 2 (2022)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

The human body does not have an enzyme that can break down uric acid, so the accumulation of uric acid can cause disease. This problem can be overcome by uricolytic therapy by utilizing the activity of the uricase enzyme. In this study, the uricase enzyme was extracted from goat liver by optimizing the centrifugation speed and the extraction pH. The purpose of the optimization is to get maximum uricase activity. Uricase extraction to optimize centrifugation speed using borate buffer pH 8.5 then centrifuged at 7.000; 9.000; 11.000; 13.000 and 15.000 rpm with a temperature of 4oC. Furthermore, pH optimization was carried out using pH buffers 6, 7, 8, 9, 10 and 11 by centrifuging the optimum speed obtained. The crude extract obtained was further tested for its enzyme activity . The results showed that the highest uricase activity was achieved if the extraction was carried out at pH 8 using centrifugation at an optimum speed of 13,000 rpm. The higher uricase activity indicates that the extracted uricase concentration is increasing.

Growth Pattern and Degradation Activity of Caffeine-degrading Bacteria Consortium Suksma, Nadhea Ayu; Utarti, Esti; Arimurti, Sattya
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.32609

Caffeine-degrading bacteria can be used as agents to degrade caffeine, thereby reducing the concentration of caffeine in organic waste. The decomposition process is carried out by a single bacterium or a consortium of bacteria. Caffeine-degrading bacteria from Sempol, Bondowoso, namely Acinetobacter gerneri KAFS 47, Paracoccus denitrificans KAFS 16 and Pseudomonas plecoglossicida KAFS 34, could be used as a bacterial consortium to promote caffeine degradation. The aim of this study was to analyze associations between caffeine-degrading bacteria isolates, bacterial resistance to antibiotics, growth patterns, and caffeine degradation of a consortium of caffeine-degrading bacteria, and the correlation of bacterial growth with caffeine degradation. The research method used is an analysis of the association between isolates, the development of bacterial consortium growth patterns, and their analysis based on antibiotic resistance, patterning of caffeine degradation, and correlation test (Pearson) of bacterial growth with caffeine degradation. The result of the association test between bacteria showed that the three bacteria had the potential to be used as a consortium of caffeine-degrading bacteria. A. Gerneri, P. denitrificans, and P. plecoglossicida were resistant to the antibiotic cefixime (100 ppm), erythromycin (50 ppm), lincomycin (50 ppm), metronidazole (50 ppm), and sanprima (50 ppm). The growth of the bacterial consortium (54.779 CFU/mL) was higher than that of P. plecoglossicida (49.277 CFU/mL) and lower than that of A. gerneri (93.481 CFU/mL) and P. denitrificans (84.940 CFU/mL) in incubation time of 4 days. However, the consortium of bacteria and P. plecoglossicida were able to degrade caffeine 24 hours faster (3 days) than the other two single isolates (4 days) to degrade 2.5 g/L caffeine in media to 0%. Bacterial growth due to caffeine degradation has a perfect correlation value (>0. 950) and is negative.

Using Lignosellulose Waste as a Xylanase Production Media of Mold Isolated from Rice Straw of Coastal-field Utarti, Esti; Siswanto, S.
Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Hemicellulose is one of lignocellulose waste component, so that xylanase is one of importance enzyme of lignocellulose waste biodegradation. Molds as main decomposer lignosellulose waste has enzyme activities higher than yeast and bacteria. The aim of the research is to find mold that have xylanolitic activity using lignocellulose waste as media production. The research consist of isolations and screening mols from coastal-field of watu Ulo Jember, xylanase production using lignocellulose waste and idntification of mold which has the highes xylanase activity. A total of 66 molds isolated from rice straw in coastal-field of Watu Ulo Jember. There were screened for their xylanase activity. In semiquantitatively screen on Oat Spelt Xylan plate, the result showed that 62 have xilanolytic activities. Based on clearing zone production, isolates ESW A1 (3.2), ESW A5 (3.1), ESW C 16 (3.26), ESW D4 (3.0) and ESW D15 (3.21) have xilanase activity index higher than others. Furthermore, quantitative analysis using wheat bran, rice straw and baggase in basic salt Mandel’s modification media showed that xylanase activity of isolate ESW D4 was higher on rice straw 3% as substrate production with activity 2.66 U/mL. Isolate ESW D4 identified as Aspergillus foetidus so that called as Aspergillus foetidus ESW D4. Keywords: rice straw, coastal-field, Aspergillus foetidus ESW-D

Identifikasi Aktinomiset Selulolitik dan Xilanolitik Indigenous Utarti, Esti; Suwanto, Antonius; Suhartono, Maggy T; Meryandini, Anja
BERKALA SAINSTEK Vol 8 No 1 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i1.15941

Lignoselulosa merupakan penyusun utama dinding sel tumbuhan, sehingga keberadaannya berlimpah di alam. Aktinomiset indigenous yang memiliki aktivitas selulolitik dan xilanolitik ekstraseluler berpeluang sebagai agens biokonversi limbah berlignoselulosa menjadi produk bermanfaat. Penelitian ini bertujuan untuk mendapatkan aktinomiset potensial yang memiliki aktivitas selulolitik dan xilanolitik. Penelitian ini diawali dari isolasi dan pemurnian aktinomiset indigenous asal lahan perkebunan kelapa sawit dan Taman Nasional Bukit Duabelas Jambi.Tahapan selanjutnya adalah penapisan aktivitas selulolitik dan xilanolitik dari aktinomiset, uji pertumbuhan aktinomiset pada mikrokristalin selulosa, dan identifikasi aktinomiset potensial berdasarkan karakter morfologi, fisiologi dan biokimia. Hasil penelitian menunjukkan bahwa aktinomiset isolat S2 yang diisolasi dari lahan perkebunan kelapa sawit mempunyai aktivitas selulolitik dan xilanolitik lebih baik dari keempat isolat lain. Aktinomiset isolat S2 juga mampu tumbuh secara lebih baik pada mikrokristalin selulosa. Aktivitas selulolitik, xilanolitik dan kemampuan tumbuh pada mikrokristalin selulosa dari aktinomiset isolat S2 menunjukkan potensinya sebagai agens pendegradasi material belignoselulosa. Berdasarkan identifikasi morfologi, fisiologi dan biokimia, aktinomiset isolat S2 tergolong dalam genus Streptomyces.

Co-Authors AA. Istri Ratnadewi Achmad Sjaifullah Andani, Kadek Novi Anggitasari, Dhanti Fatma Anja Meryandini Antonius Suwanto Ayuningtyas, Tantri Raras Azhari, Fitri Bambang Sugiharto Belkis, Malika Dina Amalia Syahidah Dwi Setyati Edia F.D Edia, Edia F.D Eva Tyas Utami Evi Umayah Ulfa Farrennina, Tasya Preira Ferymon Mahulette Hafan Sutawardana, Jon Handoko, Dhimas Rizky Ida Ayu Putu Sri Widnyani Izzay Afkarina Jefri Nur Hidayat Kahar Muzakhar Kartika Senjarini Lina Nurita Maggy T Suhartono Mashuri Mashuri Medayani, Rani Dian Media Ningtyas, Rinda Mulia Hakam Nashrullah, Shafa Nistiandani, Ana Nur Widayati Purniasari, Fina Yunita Putri, Amelia Fahreza Rendy Setiawan Riki Juni Krismiadi Rondhianto Rondhianto S Siswanto S. Siswanto Safira Isti’nafil Islam Saniyah Fatkhul Alim Sattya Arimurti Shafa Nashrullah Siswanto Siswanto Siswanto Siswanto Siswoyo Siswoyo Suksma, Nadhea Ayu Susantin Fajariyah Sutoyo Sutoyo Sutoyo Sutoyo Sutoyo Sutoyo Su’udah Hasanah Syafira Lailatul Ulfa Marfuah Tasya Preira Farrennina Tri Santi Kurnia umniyyah, zahratul Wijayanti, Sinta Winarsa, Rudju Wuryanti Handayani

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