Sunarso Suyoso
Departemen/Staf Medik Fungsional Ilmu Kesehatan Kulit dan Kelamin Fakultas Kedokteran Universitas Airlangga/Rumah Sakit Umum Daerah Dr. Soetomo Surabaya

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Parker ink-KOH stain, Chicago Sky Blue (CSB) stain, and Fungi Culture, for The Diagnosis of Superficial Dermatomycoses Sunarso Suyoso; Anggraeni Noviandini; Linda Astari
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 29 No. 1 (2017): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.835 KB) | DOI: 10.20473/bikk.V29.1.2017.21-29

Abstract

Background: Superficial dermatomycoses are infections of skin, nails, and hair that can be divided into dermatophyte, pityriasis versicolor (PV), and candidiasis based on the causative pathogens. Rapid diagnosis is important to initiate the treatment earlier. To establish the diagnosis, direct microscopy using potassium hydroxide and culture examinations could be performed. Although the routine examination using Parker ink-KOH staining could be done in very short time, it was lacking of color contrast and requiring considerable skill interpretation. Various contrast dyes are available including a new contrast Chicago Sky Blue (CSB) staining. Purpose: To evaluate the result of Parker ink-KOH stain, CSB stain, and culture for the diagnosis of superficial dermatomycoses. Methods: The study was an observational descriptive research. Skin scrappings from patients with clinical diagnosis of superficial dematomycoses in Dermatology and Venereology Outpatient Clinic Dr. Soetomo General Hospital were examined using Parker ink-KOH stain, CSB stain, then interpreted by a researcher and analysts. The samples were also cultured. Results: A total of 45 samples, 71.1% revealed dermatophyte patients, 22.2% PV patients, and 6.7% candidiasis patients. The fungal filaments were detected in Parker ink-KOH stain by researcher 91.11% of the samples and by analysts 95.56%. CSB stain were detected 100% in all the samples by both observers. The culture was positive in 71.1% samples. Conclusion: CSB stain provides a good color contrast and shown a promising examination as it is rapid, simple, and easy to interpret for the diagnosis of superficial dermatomycoses, thus it is suitable to apply for inexperienced clinicians in dermtology clinical setting and laboratory.
Profile of Fungal and Bacterial Infections in Inguinal Erythrosquamous Dermatoses Irma Tarida Listiyawati; Sunarso Suyoso; Rahmadewi Rahmadewi
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 29 No. 3 (2017): DESEMBER
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.28 KB) | DOI: 10.20473/bikk.V29.3.2017.204-211

Abstract

Background: Erythrosquamous dermatoses are diseases of the skin, characterized with the erythematous changing accompanied with scales. Usually, the patients were treated with antifungal and antibiotic, but there were no data to support this therapy. Purpose: To describe the profile of fungal and bacterial infections in erythrosquamous dermatoses. Methods: This is a descriptive cross-sectional study in patient with inguinal erythrosquamous dermatoses in Dermatology and Venereology Outpatient Department, Dr. Soetomo General Hospital 2016. Subjects have been collected through consecutive sampling, and the amount of subject were determined  as total sampling from May – August 2016 Results: The profile of fungal infections were tinea kruris (57.14%) and intertriginous candidiasis (9.52%). Fungal infections as a trigger were seborrheic dermatitis (14.29%) and erythema intertrigo (14.29%). Profile of bacterial infections were erythrasma (4.76%). Result of fungal culture were T. mentagrophytes (52.38%), C. parapsilosis (9.52%), S. ciferii (4.76%), C. albicans (4.76%), T. inkin (4.76%), Malaszesia spp (9.52%), and negative result (14.28%). Result of gram positive bacterial culture were S. aureus (38.10%), S. epidermidis (9.52%), S. lentus, S. haemolyticus, S. hominis 4.76%. Mixed gram positive bacteria were S. aureus-S. haemolyticus dan S. lentus-E. faecalis 4.76%. Gram positive and gram negative bacteria were S. aureus-A. baumanii, S. aureus-S. haemolyticus-A. baumanii 4.76% each. Gram negative bacteria were A. baumanii  and  K. pneumoniae 4.76% each and  contamination 9.52%. Conclusion: The profile of fungal and bacterial infections in inguinal erythrosquamous dermatoses mostly were tinea cruris. The result of fungal culture mostly were T. mentagrophytes. The result of bacterial culture mostly were gram positive bacteria, consist of S. aureus, S. epidermidis, S. lentus, S. haemolyticus, S. hominis, E. faecalis.