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Evaluation of genetic diversity of Katingan catlle and their genetic relationship with some other local catlle through DNA microsatellite analysis Ngaji Utomo, Bambang; Noor, R.R.; Sumantri, c.; Supriatna, I; Gurnadi, E.D.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

Katingan cattle is one of Indonesian animal genetic resources located in Katingan District, Central Kalimantan. The Katingan cattle is predicted to be extint, therefore it is necessary to conserve the cattle. Breed characterization is the primary step in any conservation program. Characterization was done using 10 microsatellite markers to evaluate the genetic diversity of Katingan Cattle in three subpopulations and their genetic relationship with some other local cattle. A total number of 72 random whole blood of Katingan samples consisted of Pendahara (20 samples), Buntut Bali (20 samples), and Tumbang Lahang (32 samples) were used. The samples of Bali, PO, and Limousine Cattle were 11, 6, and 3 respectivelly. The number of 136 aleles were found with the variation from 9 aleles (ILSTS089) to 18 aleles (ILSTS013) and the average of the alele was 13.6 aleles per locus. A number of alel from Tumbang Lahang was higher (10.8 aleles) than Pendahara (10.4 aleles) and Buntut Bali subpopulation (7.3 aleles). Some loci produced polymorphic diagnostic aleles which varied from 1-7 types of allele. HEL013 and BM1818 had four aleles, while ILSTS026 and ILSTS089 had five and six aleles respectively. ILSTS029 and ILSTS036 had seven aleles. The diagnostic aleles were also found in Tumbang Lahang subpopulastion, as well as in Pendahara, and Buntut Bali. Heterozigositas values of Pendahara, Buntut Bali, and Tumbang Lahang subpopulation were 0.454, 0.478, and 0.529 respectively. While the average of heterozigositas (Ä¤) was 0.492. Subpopulation of Tumbang Lahang was closer genetically to Pendahara (0.169) than Buntut Bali (0.173) and also the subpopulation was closer genetically to PO cattle (0.259) when compared to Buntut Bali (0.311) and Pendahara (0.329). The population of Katingan cattle was within one kluster with PO Cattle. Key Words: Katingan Cattle, Microsatellite, Genetic Diversity

Oil palm by product as an alternative feedstuff in Central Kalimantan: 1. The effect of feeding palm oil mill effluent on broiler’s performance Ermin Widjaja; Wiranda G Piliang; Iman Rahayu; Bambang Ngaji Utomo
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Palm oil mill effluent, a by product of crude palm oil processing, is found in an exessive amount in Central Kalimantan. It is estimated that 400 ton of this material is produced per day by crude palm oil (CPO) factory in this region. This material contains 12.63-17.41% crude protein, 9.98-25.79% crude fiber, 7.12-15.15% crude fat and 3217-3454 kcal/kg (gross energy). An experiment was conducted on broiler chickens of Hubbard strain in Kapuas District. The objectives of the experiment was to study the performance of broilers fed solid in the diet. The experiment was arranged in a completely randomized design. The treatments were levels of solid in the diet, i.e., 0.0, 12.5, 25.0 and 37.5%. Four hundred broilers were devided into 4 treatment groups with 5 replications, consisted of 20 chickens in each replicate. The results of this experiment showed that diet containing 0.0, 12.5, 25.0 and 37.5% of solid respectively produced final live weight of broilers 2508, 2229 and 1880 g respectively, whereas control (0.0% of solid) was 2712 g. Feed conversion of the treatments were 2.39, 2.76, 3.24 respectively and without solid as control was 2.36. It is concluded that diet containing as much as 12.5% of solid can be used in broiler ration. Key Words: Solid, Broiler Chickens, Performance

The qualitative variation of Katingan cattle Bambang Ngaji Utomo; R.R. Noor; C Sumantri; I Supriatna; E.D Gunardi; B Tiesnamurti
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The characterization is main activities in order to identify important trait of Katingan cattle. Information of its phenotypic characteristic is not available yet. The aim of the study is to describe the qualitative characteristic of Katingan cattle. Three location of the study were sub-district of Tewah Sanggalang Garing (Pendahara Village), sub-district of Pulau Malan (Buntut Bali Village), and sub-district of Katingan Tengah (Tumbang Lahang Village). The samples of Katingan cattle were taken as many as possible based on the field conditions to observe pattern of colour, growth of horn and gibbosity appear among two horns. General characteristic of Katingan cattle was various in colours of coat, having horns, humped and dewlop. The main characteristics were expressed at female of Katingan cattle. There were six variations of horn shape at female, however the growth of arching forwards was dominant (78.4%). Horn shape of male was generally grown upside (98.3%). Gibbosity was founded among horn only at female cattle (92.6%). There were 9 combinations of coat colour of female Katingan Cattle, those were brown reddish (27%), brown whitish (14.1%), brown like colour of Bali cattle (13.8%), black (12.5%), brown dull (9.6%), brown sorrel (9.3%), blackish (7.1%), white brownish (5.5%) and white grayish (4.5%). Male of Katingan cattle had eight colour combinations, those were brown whitish (14.8%), brown whitish and reddish (14.8%), brown reddish (13.1%), blackish (12.3%), brown whiteish with black withers (10.7%), brown sorrel (9.8%) and brown sorrel with black withers (7.8%). Based on qualitative analysis, the Katingan cattle had variations in coat of colour, growth of horn and the gibbosity. The variation of coat colour had potentially selection related to the value of cultural. Key Words: Katingan Cattle, Qualitative, Coat Colour, Horn, Gibbosity

Evaluation of genetic diversity of Katingan catlle and their genetic relationship with some other local catlle through DNA microsatellite analysis Bambang Ngaji Utomo; R.R. Noor; c. Sumantri; I Supriatna; E.D. Gurnadi
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Katingan cattle is one of Indonesian animal genetic resources located in Katingan District, Central Kalimantan. The Katingan cattle is predicted to be extint, therefore it is necessary to conserve the cattle. Breed characterization is the primary step in any conservation program. Characterization was done using 10 microsatellite markers to evaluate the genetic diversity of Katingan Cattle in three subpopulations and their genetic relationship with some other local cattle. A total number of 72 random whole blood of Katingan samples consisted of Pendahara (20 samples), Buntut Bali (20 samples), and Tumbang Lahang (32 samples) were used. The samples of Bali, PO, and Limousine Cattle were 11, 6, and 3 respectivelly. The number of 136 aleles were found with the variation from 9 aleles (ILSTS089) to 18 aleles (ILSTS013) and the average of the alele was 13.6 aleles per locus. A number of alel from Tumbang Lahang was higher (10.8 aleles) than Pendahara (10.4 aleles) and Buntut Bali subpopulation (7.3 aleles). Some loci produced polymorphic diagnostic aleles which varied from 1-7 types of allele. HEL013 and BM1818 had four aleles, while ILSTS026 and ILSTS089 had five and six aleles respectively. ILSTS029 and ILSTS036 had seven aleles. The diagnostic aleles were also found in Tumbang Lahang subpopulastion, as well as in Pendahara, and Buntut Bali. Heterozigositas values of Pendahara, Buntut Bali, and Tumbang Lahang subpopulation were 0.454, 0.478, and 0.529 respectively. While the average of heterozigositas (Ĥ) was 0.492. Subpopulation of Tumbang Lahang was closer genetically to Pendahara (0.169) than Buntut Bali (0.173) and also the subpopulation was closer genetically to PO cattle (0.259) when compared to Buntut Bali (0.311) and Pendahara (0.329). The population of Katingan cattle was within one kluster with PO Cattle. Key Words: Katingan Cattle, Microsatellite, Genetic Diversity

Palm oil by-product as an alternative feedstuff in Central Kalimantan: 2. The effect of feeding palm oil mill effluent on cholesterol, fatty acid and vitamin A of broilers Ermin Widjaja; Bambang Ngaji Utomo
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Solid heavy paste, a byproduct of crude palm oil processing, is found in an excessive amount in Central Kalimantan. It is estimated that 400 tons of this material were produced per day by crude palm oil (CPO) factories in this region. This material contains 12.63-17.41% crude protein, 9.98-25.79% crude fiber, 7.12-15.15% crude fat, 3217-3454 kcal/kg (gross energy) and 1.5% CPO. Considering its composition, this material can be used as an ingridient in broiler’s diet. This study was conducted on broiler chickens of Hubbard strain in Kapuas district. The objectives of the experiment were to study the cholesterol, fatty acid and vitamin A retained in meat and liver of broilers as a result of feeding ration containing solid heavy paste. This experiment was arranged in a completely randomized design. The treatments were levels of solid in the diet, i.e., 0.0, 12.5, 25 and 37.5%. Four hundred broilers were divided into 4 treatmen groups with 5 replications, consisted of 20 chickens in each replication. Data was analyzed based on statistical and descriptive analyses. The results of this experiment indicated that 25% solid in the diet decreased the total fat and cholesterol in the meat and liver, but increased polyunsaturated fatty acid contained in meat. The highest contain of vitamin A in meat and liver was found in the group that received diet containing 12.5% solid. Key Words: Solid, Broiler Chickens, Cholesterol, Fatty Acid, Vitamin A

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