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All Journal Jurnal Teknologi Pertanian Jurnal Ilmiah Mahasiswa Pendidikan Kewarganegaraan EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Indonesian Journal of Medical Chemistry and Bioinformatics Journal of Clinical Microbiology and Infectious Diseases
Ika Ningsih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Immunology & microbiology Medicine & Pharmacology

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Feasibility study of tapioca production from dried cassava on small and medium industries Wijana, Susinggih; Nurika, Irnia; Ningsih, ika
Jurnal Teknologi Pertanian Vol 12, No 2 (2011)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The aim of this research was to find out the technical and financial feasibility ofproduced tapioca flour from cassava dried. The methods used  in this research aredescriptive and experimental. Analysis was done on the product quality, consumeracceptance test by hedonic scale (using expert panelist), and  best treatment by indexeffectivity method,  and different test between the best treatment and commercialproduct, also analysis of financial exhibit production cost and Break Event Point. Resultindicated that the best treatment  was the processing  tapioca by replacement soakingwater with value product  1,000 and average value 5,5 (like). Best product result ofassessment of consumer was included in the quality of A (middle) with tapioca flourcharacteristic was: yield 38%, water content 7,69%, starch content 76,21%, sulphiteresidual 14 ppm, ash content 0,95%, and white degree to MgSO4 was 77,49%. At theproduction capacity planned of 2,895 ton/9 months needed total cost of Rp.567.063.000,00, and at price sell of Rp. 2.749,00 /kg, yielded total of profite Rp.113.376.353,00. The value of Break Event Point (BEP)  at  volume was 18.203,88 /kg or price was  Rp. 50.042.476,00.Key word: feasibility, tapioca, dried cassava
PERKAWINAN MUNIK (KAWIN LARI) PADA SUKU GAYO DI KECAMATAN ATU LNTANG KABUPATEN ACEH TENGAH Ika Ningsih; Zulihar Mukmin; Erna Hayati
Jurnal Ilmiah Mahasiswa Pendidikan Kewarganegaraan Vol 1, No 1 (2016): Agustus 2016
Publisher : Universitas Syiah Kuala

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Abstract

Perkawinan Munik (kawin lari) adalah upaya seorang gadis yang ingin menikah kerena tidak direstui ataupun lamaran laki-laki yang ditolak, dengan cara mendatangi imam Kampung, namun saat ini perkawinan Munik (kawin lari) sudah bergeser, salah satunya telah melanggar nilai agama. Penelitian ini bertujuan untuk mengetahui bagaimanakah Perkawinan Munik (kawin lari) pada suku Gayo di Kecamatan Atu lintang Kabupaten Aceh Tengah. Penelitian ini menggunakan pendekatan kualitatif dengan jenis penelitian deskriftif. Teknik pengumpulan data menggunakan teknik wawancara dan observasi, dengan analisis data, penyajian data dan menarik kesimpulan. Subjek dalam penelitian ini sebanyak 8 orang yaitu, yang mengerti dan pernah terlibat langsung dalam Perkawinan Munik (kawin lari). Hasil observasi dan wawancara dalam penelitian menunjukkan bahwa pernah terjadi perkawinan munik (kawin lari) namun telah berubah, yaitu terdapat kasus munik (kawin lari) karena melanggar nilai agama, perkawinan munik (kawin lari) pada dasarnya untuk kedua orang yang telah sama-sama ingin menikah namun terhalang restu orangtua, namun pada saat ini perkawinan Munik (kawin lari) yang terjadi bukan lagi karena tidak mendapatkan restu, tetapi karena telah melakukan pelanggaran nilai agama, walaupun masih terdapat beberapa kasus yang dikarenakan tidak mendapatkan restu, faktor yang mempengaruhi hal tersebut adalah mulai hilangnya Norma adat yang disebut Sumang (melanggar nilai agama dan norma adat), lemahnya kontrol orang tua terhadap anak, pergaulan anak itu sendiri, kemajuan teknologi, dan salah menggunakan fasilitas yang telah diberikan oleh orang tua, seperti sepeda motor dan telepon genggam. Kemudian yang berubah pada perkawinan Munik (kawin lari) saat ini yaitu, tidak berlakunya lagi hukuman Adat terhadap pasangan muda-mudi yang melakukan perkawinan Munik (kawin lari) baik dari hukum Adat maupun hukuman dari kampung, adapun hukuman dari kampung belum terlaksana. Saran dalam penelitian ini adalah diharapakan perkawinan Munik (kawin lari) dapat kembali seperti semula, dan hukum adat dapat diberlakukan kembali, dan kepada aparatur kampung dapat lebih tegas dan bertanggung jawab terhadap tugasnya.Kata kunci: Kawin Lari (Munik), Suku Gayo
Permasalahan dan Pemeriksaan Actinobacillus Ika Ningsih; Edy Wiranto
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 7 No 2 (2022): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.348 KB) | DOI: 10.33019/ekotonia.v7i2.3727

Abstract

Actinobacillus termasuk bakteri oportunistik dan merupakan flora komensal dalam tubuh inang. Pada kondisi normal tidak menyebabkan penyakit dan infeksi yang disebabkan oleh bakteri ini terutama dipicu oleh invasi bakteri komensal inang atau bakteri dari lingkungan masuk ke jaringan tubuh inang. Sebagian besar spesies Actinobacillus ditemukan secara khas sebagai komensal pada saluran pernapasan, pencernaan dan genital dari beberapa spesies hewan maupun manusia. Bakteri ini bersifat bakteri Gram negatif yang berbentuk basil, tidak bergerak, tidak membentuk endospora, bersifat anaerobik fakultatif atau mikroaerofilik serta mampu memfermentasikan karbohidrat, mereduksi citrat, urease positif. Sebagian besar spesies Actinobacillus tumbuh lambat pada media perbenihan agar darah dan agar coklat. Oleh karena prevalensi dan virulensinya tidak setinggi bakteri oportunistik lainnya mengakibatkan jarang ditemukan kasus infeksi Actinobacillus dan kasusnya pada manusia tidak banyak dilaporkan. Oleh karena itu pemeriksaan laboratorium untuk menunjang diagnosis pasti infeksi oleh Actinobacillus sangat diperlukan. Pemeriksaan mikrobiologi antara lain dilakukan dengan uji mikroskopik, biakan, identifikasi biokimia, kepekaan terhadap antibiotik, uji molekuler seperti PCR (Polymerase Chain Reaction), Loop-mediated isothermal amplification (LAMP) dan hibridisasi DNA.
Knowledge and practice of COVID-19 pandemic prevention in a community in Jakarta Wahid, Mardiastuti H; Kiranasari, Ariyani; Ningsih, Ika; Sari, Meutia Kumala; Aziz, Mochamad Helmi
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.55

Abstract

Introduction: COVID-19 is an emerging infectious disease that causes a pandemic with high morbidity and mortality due to its wide transmission. This situation should be prevented not only by the government but also by the community. Efforts have taken place to prevent COVID-19 transmission including the obligation to wear masks, wash hands, keep a distance, stay at home, avoid crowds, and administer the vaccination. This study aims to examine the communities’ knowledge, perception, and practice in preventing the spread of the COVID-19 pandemic. Methods: This is a descriptive cross-sectional study in a suburban area in Jakarta. Ninety-seven respondents participated in this study. Data was collected by filling questionnaire via G-form and analyzed descriptively.   Results: A total of ninety-seven respondents participated in this study with aged 18-73 years. In terms of knowledge about COVID-19, the majority of the respondents know the etiology, symptoms, transmission, and the way to prevent the disease. Only less than half of them understand that corpses are capable of transmitting COVID-19. However, this research found a lack of implementation of practice in preventing COVID-19 transmission because only 62.9% of respondents always bring hand sanitizer/ABHS. Only a small percentage change clothes after leaving the house, do exercise, and take vitamins. Conclusion: Although the community has sufficient knowledge and a good perception of COVID-19 prevention, rigorous effort should be provided to enhance the quality of practice of the community, to prevent the spread of COVID-19.
Pathogenesis, Diagnosis, and Examination of Corynebacterium diphtheriae Ningsih, Ika; Safrullah, Muhammad Iqbal
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 3, No. 2
Publisher : UI Scholars Hub

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Abstract

Corynebacterium diphtheriae is a rod-shaped bacterium, Gram-positive (purple), growth requires the presence of oxygen or can live with oxygen or without oxygen, nonmotile, non-capsular, non-sporing, catalase positive. Most species ferment carbohydrates such as fructose, galactose, glucose, maltose, and mannose and produce an exotoxin called diphtheria toxin (DT) which can cause diphtheria, a respiratory infection characterized by sore throat and the production of a thick layer / gray pseudomembrane and generally affects children aged 15 years and under and is very vulnerable in people who are not immunized and in low immune systems. Diphtheria is a dangerous and life-threatening disease if not detected early, so clinical diagnosis must be made immediately. Therefore, clinical diagnosis methods must be supported by laboratory examinations to detect the bacteria. Examinations that can be performed for the diagnosis of bacteria of the genus Corynebacterium include culture examination on growth media, toxicity test/toxin identification, serology test, histology examination and imaging test, biomarker test, and PCR (Polymerase Chain-Reaction) test.
The Effect of Temperature for Producing Thayer-Martin Agar Media Using Sheep Blood on Neisseria Gonorrhoeae Growth with Various Inoculums Ningsih, Ika; Sumadiyo, Kayla Adyapratista
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 4, No. 1
Publisher : UI Scholars Hub

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Abstract

Neisseria gonorrhoeae is a Gram-negative bacterium in the form of diplococci that infects mucous membranes through direct inoculation, is fastidious, and requires special media for growth because it is susceptible to heating and dryness, so it cannot survive long outside its host. Thayer-Martin agar media is a selective culture medium for the diagnosis of Neisseria gonorrhoeae bacterial infections enriched with sheep blood. The purpose of this study was to determine the optimum temperature for various temperature variations of making Thayer-Martin agar when mixing with sheep blood, compared to the temperature according to the manufacturer's procedure. Determining this ideal temperature may help improve the accuracy of the diagnosis of Neisseria gonorrhoeae infection. This study used an experimental laboratory design. Sheep blood was mixed into Thayer-Martin agar at several different temperatures: 56°C, 65°C, 75°C, and 85°C. After agar preparation, Neisseria gonorrhoeae ATCC 43069 was inoculated onto the agar with McFarland standards of 0.5, 1.0, 2.0, and 3.0. The plates were then incubated under appropriate conditions to observe bacterial growth. Neisseria gonorrhoeae showed varying growth patterns at different Thayer-Martin agar preparation temperatures, which was confirmed using ANOVA analysis with a p-value of 0.002. At 56°C, full growth was observed consistently across all McFarland turbidity standards (0.5, 1.0, 2.0, and 3.0). At 65°C, growth varied with a combination of full and half-full growth in all standards. However, temperatures of 75°C and 85°C resulted in significantly reduced growth, showing only half-full growth in all standards. Post hoc tests revealed that agar preparation at 56°C provided the most favorable conditions for Neisseria gonorrhoeae growth compared to the other temperatures tested. This study found that 56°C provided optimal conditions for the growth of Neisseria gonorrhoeae because it resulted in maximum bacterial growth. Higher temperatures, such as 65°C, 75°C, and 85°C, resulted in reduced bacterial growth, possibly due to protein denaturation or bacterial cell damage at these temperatures.
A Comparison of The Effectiveness of Salmonella-Shigella (SS) Agar and Xylose Lysine Deoxycholate (XLD) Agar in Supporting The Growth of Salmonella sp. and Shigella sp. Ningsih, Ika; Tiro, Kharisma Winnie Chryssantium
Indonesian Journal of Medical Chemistry and Bioinformatics
Publisher : UI Scholars Hub

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Abstract

Diarrheal diseases caused by Salmonella sp. and Shigella sp. are among the most significant public health challenges worldwide, especially among children. To identify these bacteria, Salmonella-Shigella (SS) and Xylose Lysine Deoxycholate (XLD) agar media are used, which are selective and differential for Salmonella sp. and Shigella sp. Although these media share similar properties and functions for growing Salmonella sp. and Shigella sp., results from previous studies differ from those obtained in tests conducted by the Department of Microbiology, Faculty of Medicine, University of Indonesia. This laboratory-based experimental study used serial dilution with three replicates (triplicate). Based on SNI 2897 of 2008 concerning Standard Plate Count, which is considered valid at 30 -300 colonies, data with a value of 300 will be excluded, and the remaining valid data will be processed using SPSS for Windows version 29. The average growth of Salmonella typhimurium ATCC 14028 on SS agar was 417 × 106 CFU/mL, and on XLD agar was 420 × 106 CFU/mL, a difference that was not statistically significant. Then, the average growth of Shigella sonnei on SS agar was 317 × 106 CFU/mL, whereas on XLD agar it was 183 × 106 CFU/mL, a statistically significant difference. There was no difference in growth effectiveness between Salmonella sp. and Shigella sp. bacteria grown on SS and XLD agar media. Shigella sp. grew more effectively on SS agar.
Co-Authors Andi Yasmon Anis Karuniawati Ariyani Kiranasari Aziz, Mochamad Helmi Azmier Adib Conny Tjampakasari Edy Wiranto Erna Hayati Irnia Nurika Mardiastuti H Wahid Retno Kadarsih Safrullah, Muhammad Iqbal Sari, Meutia Kumala Sumadiyo, Kayla Adyapratista Susinggih Wijana Tiro, Kharisma Winnie Chryssantium Yeva Rosana Zulihar Mukmin