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All Journal Eksakta : Berkala Ilmiah Bidang MIPA Pharmaceutical Sciences and Research (PSR) Journal of Clinical Microbiology and Infectious Diseases Jurnal Kedokteran Meditek
Ariyani Kiranasari
Departemen Mikrobiologi Fakultas Kedokteran Universitas Pancasila
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Public Health

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Uji Kepekaan Bakteri Yang Diisolasi Dari Urin Penderita Infeksi Saluran Kemih (ISK) Terhadap Beberapa Antibiotika Pada Periode Maret–Juni 2008 Kumala, Shirly; Raisa, Nancy; Rahayu, Lestari; Kiranasari, Ariyani
Majalah Ilmu Kefarmasian Vol. 6, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Urinary tract infections (UTI) was an inflammatory response of the urethra when attacked by microorganisms. This disease can happen to people at all ages beginning with asymptomatic to moderately symptomatic infections. Thus far, antibiotic treat-ment was the best for curing the UTI although the chances for being resistance to the antibiotics were also high. Resistance to gram negative bacteri,a in particular, often occur with the antibiotics treatment. Our study at the Faculty of Medicine, University of Indonesia (UI), Bacterial isolation from patient urine sample was performed in the microbiology laboratory of UI. A total of 50 urine samples were collected from X patients volunteered in our study for bacterial isolation, however, only 23 bacterial isolates were successfully obtained. Study was carried out to moni-tor the susceptibility of bacterial isolate towards several types of antibiotics (ofloksazim, amoxyicillin, fosfomisin and sefepim) using Cakram disffusion method. Study re-sults demonstrated that susceptibility of oflokazim to both gram positive and nega-tive bacteria was very low while amoxycillin showed desecding trend of efficacy towards all types of bacteria. Fosfomisin and sefepim, on the other hand, demon-strated strong susceptibility to both gram positive and negative bacteria found in the isolates. Furthermore, it is very interesting to observe ofloksazim was resistant to gram postivie and negative bacteria. Collectively, these research findings strongly illustrated the susceptibility patten and resistance scale of baterial isolates towards various antibiotic tested in the study.
Rickettsia as an agent of neglected Rickettsia infection Tjampakasari, Conny Riana; Kiranasari, Ariyani; Abas, Ghina Mutiara
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.42

Abstract

Rickettsia is an obligate parasite of certain arthropods (especially ticks, mites, and ticks) and can cause serious diseases. OmpA dan OmpB is a surface protein that facilitates Rickettsia’s fast attachment and entry into the endothelium. Clinical symptoms are usually characterized by self-limited acute fever in both humans and other animals. Diseases caused by Rickettsia are endemic in almost all around the world, including Indonesia. Rickettsia diseases include epidemic typhus, murine typhus, scrub typhus, and spotted fever. Culture, nucleic acid amplification, and serological tests can be used as diagnostic examinations. The serological test is the gold standard of Rickettsial diagnostic, even though molecular tests have been developed. Diagnostic limitations mean that rickettsiosis is often overlooked as a cause of infection. Rickettsia eradication was carried out by giving tetracycline, chloramphenicol, and rifampin. Vaccines are not yet available so prevention and appropriate use of antibiotics based on the diagnostic examination will determine the success of eradication.
Knowledge and practice of COVID-19 pandemic prevention in a community in Jakarta Wahid, Mardiastuti H; Kiranasari, Ariyani; Ningsih, Ika; Sari, Meutia Kumala; Aziz, Mochamad Helmi
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.55

Abstract

Introduction: COVID-19 is an emerging infectious disease that causes a pandemic with high morbidity and mortality due to its wide transmission. This situation should be prevented not only by the government but also by the community. Efforts have taken place to prevent COVID-19 transmission including the obligation to wear masks, wash hands, keep a distance, stay at home, avoid crowds, and administer the vaccination. This study aims to examine the communities’ knowledge, perception, and practice in preventing the spread of the COVID-19 pandemic. Methods: This is a descriptive cross-sectional study in a suburban area in Jakarta. Ninety-seven respondents participated in this study. Data was collected by filling questionnaire via G-form and analyzed descriptively.   Results: A total of ninety-seven respondents participated in this study with aged 18-73 years. In terms of knowledge about COVID-19, the majority of the respondents know the etiology, symptoms, transmission, and the way to prevent the disease. Only less than half of them understand that corpses are capable of transmitting COVID-19. However, this research found a lack of implementation of practice in preventing COVID-19 transmission because only 62.9% of respondents always bring hand sanitizer/ABHS. Only a small percentage change clothes after leaving the house, do exercise, and take vitamins. Conclusion: Although the community has sufficient knowledge and a good perception of COVID-19 prevention, rigorous effort should be provided to enhance the quality of practice of the community, to prevent the spread of COVID-19.
Function of Moringa oleifera Lamk Leaf Extract as an Antiseptic for Pseudomonas aeruginosa and Staphylococcus aureus using Percentage Kill Method Kiranasari, Ariyani; Tjampakasari, Conny Riana; Putri, Mariza Nabila; Pratama, Vanessa Maritza
Jurnal Kedokteran Meditek Vol 30 No 3 (2024): SEPTEMBER
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v30i3.2864

Abstract

Moringa oleifera Lamk leaf extract has an antimicrobial properties especially against fungal, parasite, Gram positive and negative bacteria by inhibiting DNA synthesis and metabolism an damaging cell walls. The aim of this study was to determine whether M. oleifera Lamk leaf extract is effective as an antiseptic against P. aeruginosa and S. aureus bacteria. Percentage Kill was used in the study to determine the percentage of  bacteria death after contact with M. oleifera Lamk leaf extract in the 1st, 2nd and 5th minutes against the control and treatment simultaneously. The test is considered to meet the standard if each contact gives a result of ≥90%. For  discovered percentage kill to P. aeruginosa bacteria in the 1st, 2nd and 5th minutes each test showed a yield of 27.12%. , 47.01%, 57.7%. In the third time of  P. aeruginosa did not reach the standard. Whereas in S. aureus bacteria, each test showed a yield of 92.36%, 95.58% and 96.45%, where it was seen that all results reach  the standards criteria of ≥90%.  M. oleifera Lamk leaf extract was not effective in eliminating P. aeruginosa for all contacts because the standard value was below 90%. Whereas for S. aureus bacteria, M. oleifera Lamk leaf extract was very effective in eliminating bacteria for all contact times ≥90% with the highest value at the 5th minutes (96.45%).
Mobile Genetic Elements Associated with Third-Generation Cephalosporin Resistance in Klebsiella pneumoniae Enggel, Yulia; Tjampakasari, Connny Riana; Kiranasari, Ariyani
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 02 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss02/502

Abstract

Klebsiella  pneumoniae is the Enterobacteriaceae family can be found in the environment, humans and animals. These bacteria are common causes of hospital, community and healthcare associated infections. Third generation cephalosporin (3GC) is one of the broad spectrum cephalosporin antibiotic that is commonly used to treat this infection. Use of antibiotics without appropriate sensitivity guidance, natural antibiotic resistant bacteria and MGE mediated horizontal gene transfer have led to increased resistance in 3GC. Mobile genetic elements such as insertion sequences (IS), transposons, integrons and resistance plasmids facilitate horizontal gene transfer between bacteria. This element can move between chromosomes or plasmids, transferring genetic material to the recipient bacteria. Horizontal gene transfer can occur by conjugation, transformation, transduction and vesiduction. IS, transposons, integrons and resistance plasmids associated with 3GC resistance are discussed in this article. We analysed the role of these MGEs in 3GC resistance in K. pneumoniae using PRISMA methods from different academic sources. We found an association of MGE with ESBL and AmpC betalactamase gene. This element promotes the transfer of resistance genes to other bacteria. Understanding the MGEs that play a role in the spread of antibiotic resistance genes in K. pneumoniae is important to control the spread of the gene.
Morphological Characteristics and Differentiation of Acinetobacter baumannii Colony on Selective Medium CHROMagar Acinetobacter Wiranto, Edy; Kiranasari, Ariyani; Kusumawati, Lia; Mutiara; Tania, Tryna
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 01 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss01/482

Abstract

Acinetobacter is an opportunistic pathogen that causes nosocomial infections and is one of the pathogens with the highest prevalence in the world. In addition to its prevalence, the number of isolates that are resistant to antibiotics is increasing every year worldwide. Identification of these bacteria in patients is generally carried out using various methods however, each method has drawbacks such as a long time, low accuracy, and requires high costs. To overcome this, CHROMagar Acinetobacter is used and is expected to be one of the solutions for identifying Acinetobacter in patient samples due to its selectivity and accuracy. However, in this research, several non-Acinetobacter bacteria are able to grow in this media, making identification of A. baumannii using this media remain a challenge. Using phenotypic tests, 5 isolates were successfully separated from 32 A. baumannii isolates in this study (13,51%), where these 5 isolates showed the same characteristics as A. baumannii phenotypically on CHROMagar Acinetobacter results but differ in biochemical tests. Therefore, false-positive results will be obtained based solely on CHROMagar Acinetobacter results. In sum, the use of CHROMagar Acinetobacter must be followed by other conventional tests to increase the accuracy of Acinetobacter identification in specimens.
Co-Authors Abas, Ghina Mutiara Aziz, Mochamad Helmi Conny Tjampakasari Enggel, Yulia Ika Ningsih Kusumawati, Lia Lestari Rahayu Mardiastuti H Wahid Mutiara Nancy Raisa, Nancy Pratama, Vanessa Maritza Presialia, Athira Putri, Mariza Nabila Retno Kadarsih Sari, Meutia Kumala Shirly Kumala Tania, Tryna Tjampakasari, Connny Riana Tjampakasari, Conny Riana Wiranto, Edy Yeva Rosana