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Vita Nirmala
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Journal : Nexus Biomedika

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Deteksi Gen P16 pada Sampel Blok Parafin Lidah Penderita Karsinoma Sel Skuamosa Rongga Mulut di RSUD Dr. Moewardi. Ernestine, Coraega Gena; Subiyantoro, Pradipto; Nirmala, Vita
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Oral squamous cell carcinoma is a part of the cancer in the head and neck which ranks 10th most cancers in the world with a wide geographical distribution. It is usually found in the late stage. P16 gene (CDKN2a/INK4a) is an important tumor suppressor gene  in carcinogenesis. This study aimed to detect the expression of p16 gene on paraffin block samples of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. Methods: DNA product used in this study was isolated from 14 samples on Paraffin Blocks of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. The DNA products, then, were used as a template for PCR amplification of the p16 gene. Primers were designed with the base sequences deposited in Gene Bank. PCR amplification products were run in agarose gel electrophoresis and visualized to detect the presence of the p16 gene. Later, the results were analyzed and presented in descriptive data. Results: Only 6 of 14 samples were diagnosed as oral squamous cell carcinoma. From 6 samples only one was detected positive expression of p16 gene. DNA bands were detected in 3% agarose gel. The intensity of DNA bands was visualized lowly and there was a smear. On the 5 other samples, DNA bands were detected under the marker, thus they showed a negative result or the expression of p16 gene was not detectable. Conclusions: Among 6 samples of the tongue of the oral squamous cell carcinoma patients, only one showed positive expression of p16 gene. Errors in the molecular and tumor differentiation level analysis was able to cause the negative results in this study. Therefore further research that detected expression of p16 gene on the tongue of the oral squamous cell carcinoma patients was needed. Keywords:P16 gene, Paraffin Block, oral squamous cell carcinoma.
Deteksi Gen TIMP-2 pada Karsinoma Sel Skuamosa Lidah dari Spesimen Blok Parafin Anshorie, Muhammad Rama; ., Pradipto subiyantoro; Nirmala, Vita
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background : Tongue cancer is the most common intraoral site of cancer occurs throughout the world. When it is diagnosed at early stages of development, the tongue cancer has 80 to 90 % survival rate. Although it has a high survival rate, most patients were diagnosed at a late stage. The purpose of this study was to detect the gene as an inhibitor of cancer cell metastasis factor in a paraffin block sample. Method : This research utilized DNA isolated from 14 parafin blocks which served as samples. First, the DNA were amplified using PCR with appropiate primer. Then electrophoresis were performed to detect the presence of TIMP-2 gene in all of samples. Result : TIMP-2 gene was not detected in all of 14 samples. Summary : This research showed that TIMP-2 gene was not found on agarose gel, indicating that TIMP-2 gene was not present in all of the samples. 
Deteksi Gen TIMP-2 pada Karsinoma Sel Skuamosa Lidah dari Spesimen Blok Parafin Muhammad Rama Anshorie; Pradipto subiyantoro .; Vita Nirmala
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background : Tongue cancer is the most common intraoral site of cancer occurs throughout the world. When it is diagnosed at early stages of development, the tongue cancer has 80 to 90 % survival rate. Although it has a high survival rate, most patients were diagnosed at a late stage. The purpose of this study was to detect the gene as an inhibitor of cancer cell metastasis factor in a paraffin block sample. Method : This research utilized DNA isolated from 14 parafin blocks which served as samples. First, the DNA were amplified using PCR with appropiate primer. Then electrophoresis were performed to detect the presence of TIMP-2 gene in all of samples. Result : TIMP-2 gene was not detected in all of 14 samples. Summary : This research showed that TIMP-2 gene was not found on agarose gel, indicating that TIMP-2 gene was not present in all of the samples.
Deteksi Gen P16 pada Sampel Blok Parafin Lidah Penderita Karsinoma Sel Skuamosa Rongga Mulut di RSUD Dr. Moewardi. Coraega Gena Ernestine; Pradipto Subiyantoro; Vita Nirmala
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Oral squamous cell carcinoma is a part of the cancer in the head and neck which ranks 10th most cancers in the world with a wide geographical distribution. It is usually found in the late stage. P16 gene (CDKN2a/INK4a) is an important tumor suppressor gene in carcinogenesis. This study aimed to detect the expression of p16 gene on paraffin block samples of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. Methods: DNA product used in this study was isolated from 14 samples on Paraffin Blocks of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. The DNA products, then, were used as a template for PCR amplification of the p16 gene. Primers were designed with the base sequences deposited in Gene Bank. PCR amplification products were run in agarose gel electrophoresis and visualized to detect the presence of the p16 gene. Later, the results were analyzed and presented in descriptive data. Results: Only 6 of 14 samples were diagnosed as oral squamous cell carcinoma. From 6 samples only one was detected positive expression of p16 gene. DNA bands were detected in 3% agarose gel. The intensity of DNA bands was visualized lowly and there was a smear. On the 5 other samples, DNA bands were detected under the marker, thus they showed a negative result or the expression of p16 gene was not detectable. Conclusions: Among 6 samples of the tongue of the oral squamous cell carcinoma patients, only one showed positive expression of p16 gene. Errors in the molecular and tumor differentiation level analysis was able to cause the negative results in this study. Therefore further research that detected expression of p16 gene on the tongue of the oral squamous cell carcinoma patients was needed. Keywords:P16 gene, Paraffin Block, oral squamous cell carcinoma.
Co-Authors Coraega Gena Ernestine Muhammad Rama Anshorie Pradipto Subiyantoro Pradipto Subiyantoro Pradipto subiyantoro .