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All Journal Warta IHP (Warta Industri Hasil Pertanian)
Toshiaki NISHIGAKI
1.Department of Histopathology, School of Medicine, Shinshu University 2.Research Association of Tropical Medicinal Plants
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Mutagenicity Study of Pandanus Conoideus Oil Maeda, Takanori; Miyakita, Haruka; Goto, Manami; Ito, Akemi; Wijaya, Hendra; S. Surono, Inggrid; Nishigaki, Toshiaki
Warta Industri Hasil Pertanian Vol 30, No 02 (2013)
Publisher : Balai Besar Industri Agro

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Abstract

Pandanus conoideus (buah merah or tawi) is exclusively grown in Papua island and its neighbor areas, and indigenous people have been consuming it as functional food for thousand years. We have reported safety and anti-tumor effects of Buah Merah extract oil (S.B.M) in experimental animals. However, mutagenicity or genotoxicity of S.B.M has not been evaluated. We carried out mutagenetic evaluation of S.B.M by in vitro Ames test using salmonella typhimurium  TA98, TA100, TA1535 and TA1537 and escherichia coli WR2uvrA with or withou the activation of S9 mixture. Concentrations used for this test were 313-500ug/plate. The results show that there is no increase in revertant colonies, suggesting that S.B.M has no mutagenic activities under the conditions of this study.
Determination of Alpha-and Beta-cryptoxanthins, and Alpa-and Beta Carotenes in Buah Merah Oil By HPLC-UV Detection Wada, Mitsuhiro; Fujimoto, Kaori; Nishigaki, Toshiaki; Febriyanti, Erna; Ikeda, Rie; Nakashima, Kenichiro
Warta Industri Hasil Pertanian Vol 30, No 01 (2013)
Publisher : Balai Besar Industri Agro

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Abstract

A high-performance liquid chromatography-UV detection method for determination of Alpha-and Beta-cryptoxanthins and, Alpha-and Beta-carotenes in Buah Merah oil was developed. The separation of the four carotenoids was achieved by a combination of a Handy ODS column (150x4.6 mm, i.d.) and a Develosil Combi-RP-5 (50x4.6 mm, i.d.) via a 3-port switching valve. The mobile phase used was a mixture of CH3CN/CH3OH/ethyl acetate (=68:23:9, v/v/v). The retention times of Alpha-and Beta-cryptoxanthins and, Alpha and Beta-carotenes were 18, 20 53 and 60 min, respectively. The clean-up of Buah Merah oil was performed by liquid-liquid extraction after saponification with 13.5 M KOH solution. The calibration curves of the carotenoids showed good linearity (r> 0.000. The detection limits of four carotenoids at a signal-to noise of 3 were from 0.36 to1.14 ng/mg. Furthermore, the proposed method could be successfully applied to determine the carotenoids in 10 Buah Merah oil samples. 
(Studi Efek Antitumor Buah Merah, Pandanus conoideus, Dalam In Vitro Dan In Vivo) Nishigaki, Toshiaki; Hirose, Kunitaka; S. Surono, Ingrid; Shigematsu, Hidekazu
Warta Industri Hasil Pertanian Vol 28, No 02 (2011)
Publisher : Balai Besar Industri Agro

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Abstract

Pandanus conoideus (Buah Merah) Is Exclusively Grown In Papua Island And Its Neghbour Areas And Indigenous People Has Been Utilizing Its Extract Oil (SBM)Thousand OfDear Years. We Have First Found Relatively High quantity Of Carotenods which Carotenoids Which Consists Of Alph-And Bet-Carotene As Well As Alpha-And Beta-Cryptoxanthin In SBM. Beta -Eryptoxanthin Is a Of SBM Has Not Been Well Examined.We Evaluated Antitumor Potentials Of SBM In Vitro And In Vivo.In The In Viro Study, Proliferation Of Human Non-Small Lung Cancer Cells.A549Were Inhibited By SBM At More Than 500 Hg/mL iN mtt Assay . The In Vivo Antitumor Activity Of SBM Was Evaluated Using SBM Demonstrated Significant Antitumor Effects In Ether Tumor Volumes Or Tumor Weights. Experiment Using Nude Mice In A549 Assay Demonstrated That Antitumor Effect Of SBM May Not Be Associated With Involvement. We Conclude That Buah Merah Has Higher Potentiasls In Prevention Of Cancers, Especially Lung Cancer.Key Words : Pandanus Conoideus, Buah Merah, Carotenoid, Beta-Cryptoxanthin, Sarcomal 180, Lewis Lung Cancer, Non-Small Lung Cancer A549.
Buah Merah and Papuan People Kurniaty Roreng, Mathelda; Nishigaki, Toshiaki
Warta Industri Hasil Pertanian Vol 30, No 01 (2013)
Publisher : Balai Besar Industri Agro

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Abstract

Buah merah (Pandanus Conodeis Lam) dilaporkan telah banyak dimanfaatkan atau digunakan oleh masyarakat Papua sejak ratusan tahun yang lalu, sebagai bahan pembantu pengolahan pangan dengan cara mengekstrak menjadi pasta dalam pengolahan kentang, daging dan sayuran. Buah merah diketahui banyak mengandung karoten, lemak dan vitamin E. Selain itu mengandung protein, karbohidrat dan mineral yag kecil. Minyak buah merah mengandung empat jenis karotenoid yaitu:alpha-karoten, beta-karoten, alpha-cryptoxanthin. Dalam tulisan ini akan dibahas tentang sejarah buah merah, kandungan nilai gizi dan manfaat serta keamanannya.
Determination of Alpha-and Beta-cryptoxanthins, and Alpa-and Beta Carotenes in Buah Merah Oil By HPLC-UV Detection Wada, Mitsuhiro; Fujimoto, Kaori; Nishigaki, Toshiaki; Febriyanti, Erna; Ikeda, Rie; Nakashima, Kenichiro
Warta Industri Hasil Pertanian Vol 30, No 01 (2013)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3165.175 KB)

Abstract

A high-performance liquid chromatography-UV detection method for determination of Alpha-and Beta-cryptoxanthins and, Alpha-and Beta-carotenes in Buah Merah oil was developed. The separation of the four carotenoids was achieved by a combination of a Handy ODS column (150x4.6 mm, i.d.) and a Develosil Combi-RP-5 (50x4.6 mm, i.d.) via a 3-port switching valve. The mobile phase used was a mixture of CH3CN/CH3OH/ethyl acetate (=68:23:9, v/v/v). The retention times of Alpha-and Beta-cryptoxanthins and, Alpha and Beta-carotenes were 18, 20 53 and 60 min, respectively. The clean-up of Buah Merah oil was performed by liquid-liquid extraction after saponification with 13.5 M KOH solution. The calibration curves of the carotenoids showed good linearity (r> 0.000. The detection limits of four carotenoids at a signal-to noise of 3 were from 0.36 to1.14 ng/mg. Furthermore, the proposed method could be successfully applied to determine the carotenoids in 10 Buah Merah oil samples. 
Co-Authors Dewi, Fitriya Nur Annisa Febriyanti, Erna Fujimoto, Kaori Goto, Manami Hidekazu SHIGEMATSU Hidekazu SHIGEMATSUI Hirose, Kunitaka Ikeda, Rie Ito, Akemi Kunitaka HIROSE Kurniaty Roreng, Mathelda Maeda, Takanori Miyakita, Haruka Nakashima, Kenichiro S. Surono, Inggrid S. Surono, Ingrid Shigematsu, Hidekazu Wada, Mitsuhiro Wijaya, Hendra