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Febriyanti, Erna
Balai Besar Industri Agro
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Identifikasi Kandungan Skopoletin dalam Berbagai Jenis Umbi-Umbian Wijaya, Hendra; Ramadhan Ningrum Has, Dinia; Febriyanti, Erna; Anwar, Chaerul
Warta Industri Hasil Pertanian Vol 31, No 01 (2014)
Publisher : Balai Besar Industri Agro

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Abstract

Skopoletin merupakan senyawa kumarin yang telah dibuktikan secara ilmiah mempunyai efek kesehatan terhadap manusia.  Efek kesehatan skopoletin diantaranya adalah sebagai antikanker, antioksidan, antidiabetes tipe 2, antihipertensi, antibakteri, memperbaiki memori atau daya ingat, dan pengendalian stres serta bad mood.  Berdasarkan hal tersebut, penting untuk mengetahui sumber pangan yang mengandung skopoletin.  Penelitian ini bertujuan untuk menentukan kandungan skopoletin dalam berbagai jenis umbi-umbian yang ada di Indonesia.  Metode analisis skopoletin yang digunakan adalah metode kromatografi cair kinerja tinggi-fluoresensi (KCKT-FL).  Hasil analisis menunjukkan bahwa skopoletin terdapat pada kulit dan daging ubi kayu, ubi jalar, dan ganyong.  Skopoletin tidak terdapat pada talas, garut, dan iles-iles.  Kandungan skopoletin tertinggi terdapat pada daging ubi kayu jenis Malang 4 yaitu sebesar 112.66 mg/kg (berat kering) sedangkan pada ubi jalar terdapat pada kulit ubi jalar Cilembu sebesar 69.73 mg/kg (berat kering).
Determination of Alpha-and Beta-cryptoxanthins, and Alpa-and Beta Carotenes in Buah Merah Oil By HPLC-UV Detection Wada, Mitsuhiro; Fujimoto, Kaori; Nishigaki, Toshiaki; Febriyanti, Erna; Ikeda, Rie; Nakashima, Kenichiro
Warta Industri Hasil Pertanian Vol 30, No 01 (2013)
Publisher : Balai Besar Industri Agro

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Abstract

A high-performance liquid chromatography-UV detection method for determination of Alpha-and Beta-cryptoxanthins and, Alpha-and Beta-carotenes in Buah Merah oil was developed. The separation of the four carotenoids was achieved by a combination of a Handy ODS column (150x4.6 mm, i.d.) and a Develosil Combi-RP-5 (50x4.6 mm, i.d.) via a 3-port switching valve. The mobile phase used was a mixture of CH3CN/CH3OH/ethyl acetate (=68:23:9, v/v/v). The retention times of Alpha-and Beta-cryptoxanthins and, Alpha and Beta-carotenes were 18, 20 53 and 60 min, respectively. The clean-up of Buah Merah oil was performed by liquid-liquid extraction after saponification with 13.5 M KOH solution. The calibration curves of the carotenoids showed good linearity (r> 0.000. The detection limits of four carotenoids at a signal-to noise of 3 were from 0.36 to1.14 ng/mg. Furthermore, the proposed method could be successfully applied to determine the carotenoids in 10 Buah Merah oil samples. 
Determination of Alpha-and Beta-cryptoxanthins, and Alpa-and Beta Carotenes in Buah Merah Oil By HPLC-UV Detection Wada, Mitsuhiro; Fujimoto, Kaori; Nishigaki, Toshiaki; Febriyanti, Erna; Ikeda, Rie; Nakashima, Kenichiro
Warta Industri Hasil Pertanian Vol 30, No 01 (2013)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3165.175 KB)

Abstract

A high-performance liquid chromatography-UV detection method for determination of Alpha-and Beta-cryptoxanthins and, Alpha-and Beta-carotenes in Buah Merah oil was developed. The separation of the four carotenoids was achieved by a combination of a Handy ODS column (150x4.6 mm, i.d.) and a Develosil Combi-RP-5 (50x4.6 mm, i.d.) via a 3-port switching valve. The mobile phase used was a mixture of CH3CN/CH3OH/ethyl acetate (=68:23:9, v/v/v). The retention times of Alpha-and Beta-cryptoxanthins and, Alpha and Beta-carotenes were 18, 20 53 and 60 min, respectively. The clean-up of Buah Merah oil was performed by liquid-liquid extraction after saponification with 13.5 M KOH solution. The calibration curves of the carotenoids showed good linearity (r> 0.000. The detection limits of four carotenoids at a signal-to noise of 3 were from 0.36 to1.14 ng/mg. Furthermore, the proposed method could be successfully applied to determine the carotenoids in 10 Buah Merah oil samples. 
Co-Authors Anwar, Chaerul Fujimoto, Kaori Ikeda, Rie Nakashima, Kenichiro Ramadhan Ningrum Has, Dinia Toshiaki NISHIGAKI Wada, Mitsuhiro Wijaya, Hendra