This research aims to analyze the effect of Total Asset Turnover, Liquidity, Working Capital, and Solvency on Profitability in Manufacturing Companies listed on the Indonesia Stock Exchange in the 2016-2020 period. The method that researcher used in this study is associative quantitative method. The population of this study were 193 manufacturing companies and a sample of 79 data was obtained through purposive sampling with data consisting of 5 years. The results showed that Total Asset Turnover, Liquidity, and Working Capital have significant effect on Profitability in Manufacturing Companies listed on the Indonesia Stock Exchange in 2016-2020. While Solvency has no effect on Profitability in Manufacturing Companies listed on the Indonesia Stock Exchange in 2016-2020. Keywords : Total Asset Turnover, Liquidity, Working Capital, Solvency, Profitability