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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture
R.R. Noor
Department of Animal Science and Technology, Faculty of Animal Science , Bogor Agriculture University, Jl. Agatis, Darmaga Campus, Bogor
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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ANALYSIS ON Alu-I GROWTH HORMONE (GHAlu-I) GENE IN BALI CATTLE Jakaria, J.; Noor, R.R.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.77-82

Abstract

The research was conducted to identify Alu-I locus of growth hormone (GH) gene in Bali cattle byusing 232 blood samples collected from Bali and Lombok islands. PCR-RFLP and sequencing methodswere used to detect the polymorphism and nucleotide sequence at Alu-I locus of GH gene. The resultshowed that Bali cattle from Bali island has one genotype (LL genotype), whereas Bali cattle originatingfrom Lombok island has two genotypes, namely LL and VV genotypes, respectively. The L and V allelefrequencies from Bali and Lombok islands were 1.00 and 0.00; 0.99 and 0.01, respectively. Thesequencing result of Bali cattle LL genotype showed an AGCT sequence of enzyme Alu-I restriction site.Based on polymorphic informative content (PIC) value, it can be concluded that Alu-I locus of Balicattle from Bali and Lombok islands were monomorphic and polymorphic, respectively.
THE DIFFERENTIATION OF SHEEP BREED BASED ON THE BODY MEASUREMENTS Handiwirawan, E.; Noor, R.R.; Sumantri, C.; Subandriyo, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 1 (2011): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.1.1-8

Abstract

This research was carried out to make a difference and to predict genetic distance some sheep thatare genetically related to each other based on the body size measurements. Nineteen bodies size of 323sheeps of five sheep breeds; namely Barbados Black Belly Cross (BC), Garut Local (GL), GarutComposite (GC), Sumatra Composite (SC) and St. Croix Cross (SCC) were measured. Analysis ofvariance and canonical discriminant analysis, Mahalanobis distance, plotting canonical and dendogramwere performed using PROC GLM, PROC CANDISC, PROC CLUSTER and PROC TREE of SASsoftware ver. 9.0. Index Length, Width Slope, Depth Slope, Balance and Cumulative Index of GC sheepwere significantly higher than the four other breeds. Canonical discriminant analysis successfully coulddifferentiate among the five sheep breeds. All five breed were divided into two groups: the first groupconsisted of SCC, SC and BC; and the second group consisted of the GL and GC. The results of geneticdistance estimation showed that the SCC had a value of sheep genetic distance closest to SC (10.83) andBC (27.98), while GL had the closest distance to GC (66.60). The tail width, horn base circumference,horn length (canonical 1) and variable length of the tail and body length (canonical 2) were the breeddifferentiation variable in this study.
The genetic diversity of heat shock protein 70 gene at promoter and 5' untranslated region in beef cattle Suhendro, I.; Jakaria, J.; Noor, R.R.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 2 (2021): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.2.136-144

Abstract

This study was aimed to identify genetic diversity in the promoter area and 5' UTR (untranslated region) HSP70 (heat shock protein 70) gene in several beef cattle in Indonesia. A total of 86 blood samples of Bali, Madura, PO (Peranakan Ongole), Limousine, and BB (Belgian Blue) cattle were used in this study. The extracted DNA of all blood samples was then analyzed using the direct sequencing method. The genotyping results showed the presence of five SNP (Single Nucleotide Polymorphism) in the promoter region, namely g.-393T>C, g.-343C>A, g.-202T>C and g.-69T>G and three SNPs at 5' UTR, i.e., g.19A>G, g.45C>T, and g.100_101ins30. The frequency of SNP alleles found in PO, Limousine, and BB cattle was in equilibrium, whereas in Bali and Madura cattle populations, the allele frequency was disequilibrium. In general, the level of diversity of observed alleles ranged from low to high (0.26-1.00), where SNP g.19A>G had the widest variety. It was successfully revealed in this analysis that the SNP g.-69T>G binds to both the NF-Y and CAAT box transcription factor. In addition, the 30 bp (base pair) insertions (g.100_101ins30) that were identified in Bali and Madura cattle have never been reported in previous research studies.
Co-Authors A. Saefuddin A.B.L. Ishak ACHMAD FARAJALLAH Aron Batubara B Tiesnamurti B. Tiesnamurti Bambang Ngaji Utomo Bambang Ngaji Utomo C Sumantri C. SUMANTRI C. Talib C.H. Wijaya E. Handiwirawan E.D Gunardi E.D. Gurnadi I Supriatna I. Arifiantini Jakaria Jakaria L. Budimulyati S. L.H. Prasetyo M. Doloksaribu N. Hilmia P.R. Matitaputty P.S. Hardjosworo R. Priyanto R.E. Gurnadi R.R.A. Maheswari S. Subandriyo S. Suryana Suhendro, I. T. Susanti Yayu Zurriyati