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Variation on DNA microsatellite of Holstein-Friesian dairy cows in Baturaden Dairy Cattle Breeding Center Sumantri, C; Anggraeni, A; Farajallah, A; Perwitasari, D
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Eight microsatellite loci of which the locations were three on chromosome 6 (BTA6) namely CSN 3, BM 143 and BM 415; two on chromosome 9 (BTA9) namely ETH 225 and BM 4208; and three on chromosome 10 (BTA10) namely BP 31, BM 1237 and BM 888 were used to investigate genotypic variation of Holstein-Friesian (HF) dairy cows in Baturraden Dairy Cattle Breeding Centre. Research activities were carried out through some steps involving blood collection, DNA isolation, amplification on DNA fragments by polymerase chain reaction (PCR) and separation by electrophoresis with silver staining. Frequency and heterozygosity of genes under consideration were calculated. The results showed that the eight microsatellite loci exhibited a total number of 33 alleles. Locations of those alleles were five in BM 143 and BM 4208 loci; four in BM 415, CSN 3, ETH 225, BM 1237, BM 888 loci; and three in BP 31 locus. A number of 14 out of 33 alleles might be specific alleles for HF dairy cattle in Baturraden Dairy Cattle Breeding Centre. The lowest heterozygosity per locus (Ä¥) was 0.6151 for BM 415 whilst the highest one was 0.7301 for BM 888. Additionally, the average heterozygosity for all loci (Ä¤) detected in this study was 0.6768. The genotype BB on locus BM 143 and AC on locus CSN-3 significantly affected (P<0.05) the estimated breeding value of milk yield of HF cows in this location. Key Words: Holstein-Friesian Cows, DNA Microsatellite, Allele, EBV of Milk Yield

Performances and phylogenic relationships among local sheep in Indonesia by morphological analysis Sumantri, C; Einstiana, A; Salamena, J.F; Inounu, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

The morphological discriminant and canonical analysis were carried out to estimate the phylogenic relationship and determine the discriminant variables between Indonesian local sheep of thin tail sheep from Jonggol (Bogor) and Garut and fat tail sheep from Indramayu (originated from East Java), Donggala, Madura, Kisar, Rote and Sumbawa. The number of sheep usedÂ was totally 818 heads collected from 8 populations, were Jonggol (185), Garut (74), Indramayu (100), Donggala (60), Madura (86) Kisar (231), Rote (52), and Sumbawa (30). Discriminant analysis used for body weight and body measurements were body length, wither height, chest width, chest depth, chest circumference, skull length, skull width, skull height, tail lenght, tail width, ear length and ear width. SAS package program was used to analyze the data. The results from analysis variant showed that the body weight and body measurenment of Garut sheep almost the same with sheep from Indramayu and significantly higher (P<0,05) compared to another breeds. Whereas, Kisar and Rote have body weight and body measurenment significantly lower (P<0,05) compared to another breeds. The results from Mahalanobis distance (phenogram tree) and canonical analyses showed that breeds were divided into five groups, the first group is Garut, the second is thin-tail sheep which existed in Jonggol, the third was fat-tailed sheep which existed in Kisar, Rote, and Sumbawa, the fourth is fat-tail sheep which existed in Donggala, and the fiveth was fat-tailed sheep which existed in Indramayu and Madura. The groups of sheep between Kisar and Rote had the smallest genetic distance value equal to 1,623 and groups between Donggala and Garut had the biggest genetic distance value equal to 7,994. The highest similarity between individual inside the group was obtained from Sumbawa (100%) and the lowest similarity was from Rote (72,41%). The results from canonical analyses showed that tail lenght, ear width, ear lenght, skull lenght, body lenght, and tail width were the most discriminant variables to determine the diferences between breeds. Key Words: Sheep, Genetic Distance, Analysis Discriminant and Cannonica

Morphometric performances of Katingan cattle in Central Kalimantan Utomo, Bambang Ngadji; Noor, R.R; Sumantri, C; Supriatna, I; Gunardi, E.D
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical

Behaviour and production traits correlation of five breeds of sheep Handiwirawan, Eko; Noor, R.R; Sumantri, C; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Information about correlations among behavioral traits and production traits may facilitate using easily measured traits to be used as alternative criteria for indirect selection to improve other traits in sheep. The purpose of this study was to estimate correlations among behavioral and production traits of five breeds of sheep.Â A total of 128 head of weaned lambs and 168 ewes with nursing lambs of five breeds were used in this study, i.e. Barbados Black Belly Cross (BC), Composite Garut (KG), Local Garut (LG), Composite Sumatra (KS) and St. Croix Cross (SC). Temperament was assessed in a test arena, for subsequent association with growth rate and ewe productivity. Analysis of variance of production and behavioral traits among breeds was carried out using PROC GLM of SAS software Ver. 9.0.Â PROCCORR was used to estimate correlations among production and behavioral variables. Weaned lambs that were more docile towards the observer had higher daily gains.Â Ewes that bleated more frequently when separated from their lamb had higher lamb weaning weights and lamb survival than ewes bleating fewer times under the same conditions. In BC ewes, a positive correlation was observed between frequency of bleats and lamb survival. Key Words: Sheep, Behavior, Growth rate, Ewe productivity

The qualitative variation of Katingan cattle Utomo, Bambang Ngaji; Noor, R.R.; Sumantri, C; Supriatna, I; Gunardi, E.D; Tiesnamurti, B
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 1 (2012)
Publisher : Indonesian Animal Sciences Society

The characterization is main activities in order to identify important trait of Katingan cattle. Information of its phenotypic characteristic is not available yet. The aim of the study is to describe the qualitative characteristic of Katingan cattle. Three location of the study were sub-district of Tewah Sanggalang Garing (Pendahara Village), sub-district of Pulau Malan (Buntut Bali Village), and sub-district of Katingan Tengah (Tumbang Lahang Village). The samples of Katingan cattle were taken as many as possible based on the field conditions to observe pattern of colour, growth of horn and gibbosity appear among two horns. General characteristic of Katingan cattle was various in colours of coat, having horns, humped and dewlop. The main characteristics were expressed at female of Katingan cattle. There were six variations of horn shape at female, however the growth of arching forwards was dominant (78.4%). Horn shape of male was generally grown upside (98.3%). Gibbosity was founded among horn only at female cattle (92.6%). There were 9 combinations of coat colour of female Katingan Cattle, those were brown reddish (27%), brown whitish (14.1%), brown like colour of Bali cattle (13.8%), black (12.5%), brown dull (9.6%), brown sorrel (9.3%), blackish (7.1%), white brownish (5.5%) and white grayish (4.5%). Male of Katingan cattle had eight colour combinations, those were brown whitish (14.8%), brown whitish and reddish (14.8%), brown reddish (13.1%), blackish (12.3%), brown whiteish with black withers (10.7%), brown sorrel (9.8%) and brown sorrel with black withers (7.8%). Based on qualitative analysis, the Katingan cattle had variations in coat of colour, growth of horn and the gibbosity. The variation of coat colour had potentially selection related to the value of cultural. Key Words: Katingan Cattle, Qualitative, Coat Colour, Horn, Gibbosity

Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 â exon 6) genotypes variation Dagong, Muhammad Ihsan Andi; Herman, R; Sumantri, C; Noor, R.R; Yamin, M
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 1 (2012)
Publisher : Indonesian Animal Sciences Society

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep

Polymorphism of Calpastatin gene and its effect on body weight of local sheeps Sumantri, C; Diyono, R; Farajallah, A; Inounu, I
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

The objectives of this research were to identify polymorphism of calpastatin gene and to investigate any association of calpastatin genotype on body weight of local sheeps. A total number of DNA samples were collected from 288 heads of local sheeps from 8 populations. Two local sheep samples were medium tail sheeps (MTSs) of a Garut fighting type from Ciomas/Bogor (29) and a Garut meat type from Margawati (29). The remaining six local sheep population were one thin tail sheep (TTS) from Jonggol (36); and five fat tail (FTSs) from Indramayu (43), Madura (43), Sumbawa (26), Rote (36) and Donggala (46) respectively. Genomic DNAs of those blood of local sheeps were extracted by a standard phenol-chloroform protocol and amplified using a polymerase chain reaction (PCR) technique. PCR reaction was carried out in a thermocycler (Takara PCR of Thermal Cycler MP4) and PCR products were digested with Msp 1 enzyme restriction using a Restriction Fragment Length Polymorphism (RFLP) technique. The PCR-RFLP products were separated at 8% polyacrylamide gel electrophoresis (PAGE).Â A silver-staining method then was applied to detect fragments. Genetic variations between local sheep populations were calculated based on frequencies of genotypes and alelles. The association between genotype of calpastatin gene and body weight of local sheeps were calculated by General Linear Model method by SAS version 6.12. A length of 622 base pairs (bp) of the calpastatin gene of the Indonesian local sheeps was successfully amplified by the PCR technique. An MspI restriction enzyme cut the PCR product into two different length fragments, those were 336 bp and 286 bp designated as M allele of the CAST-Msp1; whilst that unsuccessfully cut PCR product resulted one fragment 622 bp designated as N allele of the CAST-Msp1. Locus of the CAST-Msp1 gene in most local sheeps studied was polymorphic, the exception was in the FTS from Rote of which monomorphic. The highest frequency of the M allele was in the fighting Garut sheep from Ciomas (0.29), whilst the lowest was in the FTS from Rote (0.00). However, frequencies of the M allele of FTSs from Sumbawa and Madura were similar (0.04). Further, frequencies of the M allele of local sheeps from Margawati, Jonggol, Indramayu, and Donggala were 0.24, 0.16, 0.13 and 0.12 respectively. The highest frequency of MN genotype was observed in the Garut fighting sheep from Ciomas (0.58), but the lowest was in the FTS from Rote (0.00). The heterosigosity was observed differentlyÂ among populations. The highest heterosigosity was also identified in the Garut fighting sheep in Ciomas (0.43), whilst FTSs both in Sumbawa and Madura were for the lowest (0.08). Results of this study showed that there was a definit association betwen calpastatin genotype and body weight of male sheeps from which the MN genotype significantly related to a higher body weight compared to that of the NN genotype. Key Words : Local Sheeps, Calpastatin Gene, Polymorphism, Live Weight

Identification of Single Nucleotide Polymorphism (SNP) in Mono Amine Oxidase A (MAO-A) Gene as a genetic marker for aggressiveness in sheep Handiwirawan, Eko; Noor, Ronny R; Sumantri, C; ., Subandriyo; Inounu, I
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v17i4.692

In the population, there are aggressive sheep in a small number which requires special management those specific animal house and routine management. The purpose of this study was to identify the variation of DNA marker SNP (single nucleotide polymorphism) as a genetic marker for the aggressive trait in several of sheep breed. The identification of point mutations in exon 8 of MAO-A gene associated with aggressive behavior in sheep may be further useful to become of DNA markers for the aggressive trait in sheep. Five of sheep breed were used, i.e.: Barbados Black belly Cross sheep (BC), Composite Garut (KG), Local Garut (LG), Composite Sumatra (KS) and St. Cross Croix (SC). Duration of ten behavior traits, blood serotonin concentrations and DNA sequence of exon 8 of MAO-A gene from the sheep aggressive and nonaggressive were observed. PROC GLM of SAS Ver. 9.0 program was used to analyze variable behavior and blood serotonin concentrations. DNA polymorphism in exon 8 of MAO-A gene was analyzed using the MEGA software Ver. 4.0. The results show that the percentage of the aggressive rams of each breed was less than 10 percent; except for the KS sheep is higher (23%).Â Based on the duration of behavior, aggressive sheep group was not significantly different with non aggressive sheep group, except duration of care giving and drinking behavior. It is known that concentration of blood serotonin in aggressive and non aggressive rams was not significantly different. The aggressive trait in sheep has a mechanism or a different cause like that occurs in mice and humans. In this study, aggressive behavior in sheep was not associated with a mutation in exon 8 of MAO-A gene. Key Words: Sheep, Aggressive, Exon 8 Of MAO-A Gene, Single Nucleotide Polymorphism

Identification of Body Size and Body Shape of Garut Sheep Fighting Type and Meat Type and Garut Cross Based on Principal Component Analysis Gunawan, A; Mulyono, RH; Sumantri, C
ANIMAL PRODUCTION Vol 11, No 1 (2009): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.712 KB)

This research was done to identify the body size and shape Â among three types of local sheeps of Garut fighting type, Garut meat type and their crossbred fighting and meat type in Garut district (Margawati, Sukawening and Wanaraja) and Bogor district (Ciomas and Cinagara).Â The total number of sheep from Garut district used in this study were 520 head consisting of crossbred sheep from Margawati (71 head), fighting sheep from Wanaraja (79 head), meat sheep from Wanaraja (84 head), fighting sheep from Sukawening (87 head) and meat sheep from Sukawening (62 head). Whereas the total number of sheep from Bogor district were 137 head consisting of those of fighting sheep from Ciomas (66 head) and from Cinagara (70 head). Some body measurenments measured in this study were body weight, body length, wither height, chest width, chest circumfrence, cranium, tail length and tail width.Â Analysis of all those sizes were based on principal component analysis (PCA) then visualized into the group of crowded diagram.Â Different group of crowed builded on the base of scoring in body size and body shape derivated from covarian matrix were able to identifed morphologycal penotypic differences among garut sheep studied.Â Â The results from canonicalÂ analyses showed that body lenght, chest girth,chest width, and wither height were the most discriminant variables had impact to the diferences score size between Â types of Garut sheep. However, the tail length and tail width were variables had impact to diferences score shape. The Â crowed diagram showed that an intersection among three types (fighting, meat and crossbred) of sheeps suspected as resulted from the same genetic resource, namely Garut sheep.Â Genetic flow was suspected from crossbred in Margawati to meat sheep in Cinagara as well as the sheeps from Sukawening and Wanaraja to fighting sheep in Ciomas.Â Close genetic distances that were found among fighting and meat sheeps in Sukaweining as well as fighting and meat sheeps in Wanaraja could be considered that these two groups of sheeps as the genetic resource of fighting sheep in Ciomas. (Animal Production 11(1): 8-14 (2009)Â Key Words: garut sheep, principal component analysis (PCA), body size and shape

Karakteristik morfologi dan aktivitas harian Nuri Talaud (eos histrio talautensis, muller p l s, 1776) di penangkaran Keintjem, J. R.M.; Fuah, A. M.; Sumantri, C; Masyâ€™ud, B.
ZOOTEC Vol 41, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Tujuan dari penelitian ini adalah untuk mengidentifikasi Karakteristik morfologi dan determinasi seks nuri talaud jantan dan betina dari Nuri Talaud (eos histrio talautensis) di penangkaran. Penelitian dilakukan pada bulan September 2016 hingga Maret 2017 di Badan Konservasi Alam di Manado, Sulawesi Utara. Objek yang diamati adalah sepasang burung Nuri Talaud yang telah mencapai tubuh dewasa (2,5 - 3 tahun). Jantan dan betina ditimbang setelah pengamatan bentuk tubuh, kepala dan ekor yang dipuasakan terlebih dahulu. Metode yang digunakan untuk karakteristik morfologi Nuri Talaud melihat bentuk secara visual bentuk kepala bulat, tubuh langsing dan memanjang serta bulu ekor runcing untuk jantan dan bentuk tubuh kepala tidak bulat, badan tidak langsing dan lebar dan ekor yang tidak runcing untuk betina. Karakteristik morfologi Nuri Talaud yang ditemukan dalam pemiliharaan ex situ ada empat bentuk yaitu: bentuk tubuh, bobot badan, bentuk kepala, bentuk ekor. Terdapat perbedaan bentuk antara burung Nuri Talaud jantan dan betina. Analisis morfometri terdapat delapan ukuran yaitu : panjang kepala, lebar kepala, panjang dada, lebar dada, panjang paruh atas, lebar paruh atas, tinggi paruh atas dan panjang ekor. Hasil analisis ke delapan ukuran antara jantan dan betina Nuri Talaud. Aktivitas harian yang ditemukan jantan dan betina Nuri Talaud yaitu: lokomotif, ingesti, istirahat dan perawatan tubuh dimana jantan lebih aktif dari pada betina.Â Kata kunci: Nuri talaud, karakteristik morfologi, penangkaran

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