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All Journal Jurnal SainHealth Borneo Journal of Medical Laboratory Technology SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Jurnal Pijar MIPA
Dheasy Herawati
Universitas Maarif Hasyim Latif
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics

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Journal : Borneo Journal of Medical Laboratory Technology

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Perbandingan Pengukuran Kadar Vitamin C Menggunakan Spektrofotometri UV-Vis pada Panjang Gelombang UV dan Visible Khoirul Ngibad; Dheasy Herawati
Borneo Journal of Medical Laboratory Technology Vol 1 No 2 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.616 KB) | DOI: 10.33084/bjmlt.v1i2.715

Abstract

Vitamin C is a source of antioxidants which has a primary function to inhibit the occurrence of an oxidation reaction and inhibit a free radical. The source of vitamin C can be found in food and beverage. This study aims to compare the measurement of vitamin C levels using a UV-Vis spectrophotometer at UV and Visible wavelength. The method used in this study was the UV-Vis spectrophotometry method. The analysis of vitamin C levels at UV wavelength 266 nm and Visible wavelength 494 nm with a calibration curve range 0.3 to 0.8 mg/L. Validation of the methods studied included: linearity, detection limits, quantization limits, and precision. The results showed that the measurement of vitamin C levels at UV wavelengths resulted in the linearity of concentrations of 0.2 - 0.8 mg / L, the limit of detection of 0.05 mg/L, the limit of quantization of 0.17 mg/L and level of precision with RSD to 0.2015. On the other hand, the measurement of vitamin C levels in the Visible wavelength produced linearity from concentrations of 0.3 to 0.8 mg/L, the detection limit of 0.05 mg/L, quantization limit of 0.18 mg/L and the level of precision with RSD of 1.0489. Thus, the determination of vitamin C levels in samples using a UV-Vis spectrophotometer was preferred to be measured at UV wavelengths.
Co-Authors Abdul Chamid Anton Yuntarso Didik Setio Prayitno Elsa Mega Suryani Esti Rizkiana Pratiwi Evi Dwi Aprilia Suryani Evy Ratnasari Ekawati Evy Ratnasari Ekawati Gland Konay Octavianus Istik Nuril Qomari Khoirul Ngibad Lathifa Azkhiyati Muhammad Sungging Pradana Muji Harsini Nurfadilah Nurfadilah Nurul Aziza Setyawan Setyawan Setyo Dwi Santoso Siti Nur Husnul Y. Soedaryo Soedaryo Titik Sundari Zelika Nur Jannah