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All Journal Jurnal Akuakultur Indonesia Jurnal Natur Indonesia BERITA BIOLOGI

Estu Nugroho

Pusat Penelitian dan Pengembangan Perikanan Budidaya

Author-ID : 333255

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

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KARAKTERISASI GENETIK IKAN LELE DUMBO BERDASARKAN MARKER RAPD FINGERPRINTING [Genetic Characterization of African Catfish Revealed by RAPD Fingerprinting Markers] Nugroho, Estu; Putera, Sabara
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

African catfish, namely lele dumbo, is an economically important fresh water fish in Nusa Tenggara Barat. There are three strains of African catfish that are well distributed among farmers. Genetic information of three strain catfish stocks is needed in order to sustain their development program. Genetic variations of the three strains of catfish, i.e. Sangkuriang, Masamo, Paiton has been evaluated using RAPD markers. DNA genome was extracted using phenol-chloroform methods. DNA was amplified using 20 primers (OPA 1 â 20). The results showed that three of the 20 primers have good amplification products. There was no significant genetic differences among three strains of catfish analyzed (P>0.05). Genetic variation of three African catfish stocks ranges from low to middle value. The highest genetic variation is found in strain Masamo with heterozygosity of 0.273 (with 70% polymorphism loci), followed by Sangkuriang 0.189 (60%) and Paiton0.147 (40%). Hybrid candidates of Masamo-Sangkuriang have eterozygosity ranges between 0.256 - 0.306 (with 66.6 7 â 73.77% polymorphism loci). Strain Masamo and Paiton has more close genetic relationship than among the both and Sangkuriang.

HERITABILITAS DAN PEROLEHAN GENETIK PADA BOBOT IKAN NILA HASIL SELEKSI [Heritability and Genetic gain on Weight of Tilapia Resulted Frown Individual Selection] Nugroho, Estu; Mayadi, Lalu; Budileksono, Sigit
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Directional-individual selection on tilapia growth had been conducted in West Nusa Tenggara Province. The base population had been constructed through di allele crossing between six tilapia strains i.e. NIRWANA, BEST, SULTANA, Citralada, JATIMBULAN and White Sleman. A total of 150 brood stock pairs were used for spawning with the ratio of female and male parent 1: 1 in net with size of 1x1x1 m. Two hundred larvae Â produced from each spawn pairs were communally reared in a pond for three months. Directional-individual selection was conducted at the size of > 50 grams. The cut-off for minimum selected fish was based on the lowest individual weight of 10% top population. The average value of heritability on body weight was 0.251 for males and 0.258 for the female population. The total value of genetic gain of body weight for four generations was 51.68% for male population and 56.78% for the female population.

KERAGAMAN GENETIK POPULASI IKAN NILA {Oreochromis niloticus) DALAM PROGRAM SELEKSI BERDASARKAN RAPD Arifin, Otong Zenal; Nugroho, Estu; Gustiano, Rudhy
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Objectives of the study was to discover genetic variability and genetic relationship of paternal half sib population of nile tilapia (Oreochromis niloticus) under Selection Program Scheme at Research Institute for Freshwater Aquaculture, in Bogor, West Java.Four populations from unrelated selected breeders were observed for genetic variability using RAPD. The analysis of amplification from each locus and fragments were used to estimate DNA polymorphisms, heterozygosity, fst and genetic distance.The range of heterozygosity of four examined populations was 0.1760-0.2168 with level polymorphic between 47.66% and 64.86%.The highest heterozygosity and polymorphic was on population 1 and the lowest one was on population 2. Fst test showed significance among the populations. The closest relationship was between population 1 and 4 (0.1978) and the furthest was population 2 and 3 (0.3289).

Co-Authors . Wahidah Azrita, Azrita Budileksono, Sigit Dahelmi Dahelmi Hafrijal Syandri Ida Ayu Putu Sri Widnyani K. Sumantadinata Mayadi, Lalu O. Carman Otong Zenal Arifin Putera, Sabara Ratu Siti Aliah Rudhy Gustiano

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