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All Journal HAYATI Journal of Biosciences Journal of Tropical Soils Jurnal Bioteknologi & Biosains Indonesia (JBBI)
DIANA NURANI
Laboratory of Industrial Technology Development for Agro and Biomedical Center for Bioindustrial Technology, Agency for the Assessment and Application of Technology (BPPT) BPPT Buiding II 15th floor, Jalan MH Thamrin No 8, Jakarta 10340, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology

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OPTIMASI PERMUKAAN RESPONS MEDIUM FERMENTASI Streptomyces prasinopilosus SEBAGAI ANTIFUNGI TERHADAP PATOGEN Ganoderma boninense Sunaryanto, Rofiq; Nurani, Diana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1326.321 KB) | DOI: 10.29122/jbbi.v6i2.3231

Abstract

Response Surface Optimization of Medium Fermentation for Streptomyces prasinopilosus as An Antifungal against Ganoderma boninenseGanoderma boninense is one of the pathogenic fungi that cause basal stem rot (BPB) on oil palm plants. This research aims to study the effect of carbon sources, nitrogen sources, and minerals on the production of Streptomyces prasinopilosus active compounds. Lactose, yeast extract, and minerals are medium components that show a real influence on the production of S. prasinopilosus active compounds. Optimization of the factors that have significant influence was predicted by the second-order model, statistically through a central composite design (CCD). The highest S. prasinopilosus active compound production, with a medium composition of 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution, was predicted by the quadratic model to reach 32269366.338 peak area unit on high-performance liquid chromatography (HPLC). The verification of the mathematical model of the production of the active compounds through experiments in the laboratory was 27,203,907.310 peak area unit. This result was 15.7% lower compared to the result of the quadratic model. Optimization increased S. prasinopilosus active compound 9-fold compared to that before optimization.Keywords: active compound; G. boninense; optimization; RSM; S. prasinopilosus ABSTRAKGanoderma boninense merupakan salah satu jamur patogen yang menyebabkan penyakit busuk pangkal batang atau biasa disebut BPB pada tanaman kelapa sawit. Penelitian bertujuan mempelajari pengaruh sumber karbon, sumber nitrogen, dan mineral terhadap produksi senyawa aktif S. prasinopilosus. Laktosa, yeast extract, dan mineral adalah komponen medium yang menunjukkan pengaruh nyata terhadap produksi senyawa aktif S. prasinopilosus. Optimasi terhadap faktor yang berpengaruh nyata diprediksi dengan model orde dua melalui rancangan statistis central composite design (CCD). Produksi senyawa aktif S. prasinopilosus tertinggi diprediksi oleh model kuadratik mencapai 32269366,338 luasan puncak kromatografi cair kinerja tinggi (KCKT) dengan komposisi medium laktosa 44,77 g L-1, yeast extract 13,02 g L-1, dan larutan mineral 15,95 mL L-1. Verifikasi model matematis produksi senyawa aktif yang dihasilkan melalui percobaan di laboratorium adalah sebesar 27.203.907,310 luasan puncak kromatogram KCKT. Hasil ini lebih rendah 15,7% dibandingkan dengan model kuadratik hasil optimasi. Optimasi meningkatkan senyawa aktif S. prasinopilosus 9 kali lipat dibandingkan sebelum optimasi.
The Potential Use of Wood Vinegar as an Alternative Inhibitor to Enhance 5-Aminolevulinic Acid Production by Bacillus paramycoides Farah; Fauzi, Ahmad; Monconegoro, Dicky Adihayyu; Nurani, Diana; Abraham, Silva
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.850-858

Abstract

The expensive production of 5-aminolevulinic acid (ALA) as a plant growth stimulator led to an attempt to get an alternative substrate to reduce the production cost. Levulinic acid is the inhibitor of ALA dehydratase in ALA production. Wood vinegar, a liquid gas produced from wood combustion in airtight conditions, is known to contain 12-17 mM of levulinic acid. This study aims to explore the use of wood vinegar as a potential substitute for levulinic acid in the extracellular production of ALA by bacteria identified as Bacillus paramycoides through 16S rRNA sequence analysis. Adding precursor and inhibitor glutamate and 1% wood vinegar increased the ALA production to 174.3 µM, while the combination of glutamate and levulinic acid raised the ALA production to 179.9 µM. This study confirmed that wood vinegar can enhance the concentration of ALA and potentially substitute levulinic acid as an inhibitor in ALA production.
The Influence of Bat Guano on Peat Soil Properties in the Oil Palm Plantations Bafadhal, Farah; Fauzi, Ahmad; Nurani, Diana; Monconegoro, Dicky Adihayyu
JOURNAL OF TROPICAL SOILS Vol. 28 No. 3: September 2023
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2023.v28i3.117-125

Abstract

Highlighting the role of bat guano as an organic fertilizer housing beneficial microbes for soil and plants. The research aimed to assess the effect of bat guano on bacterial diversity and chemical and nutrient properties of peat soil in the vicinity of a bat cave within oil palm plantation in West Sulawesi, Indonesia. Four samples, namely bat guano (BG), peat soil (PS), peat soil mixed with bat guano (PSM), and peat soil inside the cave (PSI) were assessed. Organic carbon, pH, total nitrogen, the C/N ratio, Cation Exchange Capacity (CEC), Base Saturation (BS), macronutrients, and micronutrients were measured across all samples. Bacteria from the samples were also isolated, and their capability in hydrolyzing urea, solubilizing phosphate, solubilizing potassium, and producing the Indole Acetic Acid (IAA) hormone was characterized. The influence of bat guano was found on the enhancement production of IAA hormone, pH, organic carbon, total nitrogen, C/N ratio, CEC, BS, macronutrients, and micronutrients. The results also demonstrated substantial bacterial community resemblance between PSM and BG in contrast to the original PS (3:1). Meanwhile, peat soil inside the cave was indicated unique and has bacterial diversity, which can hydrolyze urea, solubilizing phosphate, and potassium.
Co-Authors ABRAHAM, SILVA Bafadhal, Farah farah Fauzi Ahmad Muda Monconegoro, Dicky Adihayyu Rofiq Sunaryanto