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Isolation and Characterization of OsNAC6 cDNA from Rice (Oryza sativa L.) cv. Nipponbare, Batutegi, and Rojolele Rachmat, Agus; Nugroho, Satya; Nurdiani, Dini; Swastika, Maria; Sukma, Dewi; Aswidinnoor, Hajrial; Sudarsono, Sudarsono
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Transcription factors have an important function in regulating gene expression and plant responses to stresses. The ERF, bZIP, WRKY, MYB, and NAC are stress inducible transcription factors. The OsNAC6 is a member of the NAC transcription factor family in rice and its expression is induced by abiotic stresses, wounding and blast disease. Characterization of OsNAC6 gene sequences would give a better undersanding on how OsNAC gene functions biologically. The objectives of this research are to isolate the OsNAC6 cDNA from Nipponbare, Batutegi, and Rojolele cultivars, to characterize their DNA sequences, and to compare their sequences to other NAC genes from other plants available in GenBank DNA databases. Isolated cDNA and sequencing of the fragments resulted in a 912 bp DNA sequences. Translation of the sequences yielded a protein consisted of 303 amino acid residue. Blast analysis of amino acid sequences indicated identity of isolated cDNA from three Indonesian rice cultivars are the OsNAC6 gene. Deduced amino acid residues from amplified cDNAs of Nipponbare, Batutegi, and Rojolele cultivars shared 100% sequence identities to rice OsNAC6 (Acc. # BAA89800), 71-100% sequence identity to a number of OsNAC protein from Oryza sativa and 63-83% sequence identity to NAC protein from other plants.

Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis ARIS TRI WAHYUDI; ANDINI PURNAWIJAYA; DINI NURDIANI; TEDJA IMAS
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Acid-aluminium sensitive mutants of symbiotic bacterium Bradyrhizobium japonicum BJ11 (designated as AAS11) and KDR15 (designated as AAS15) were constructed by mini-Tn5 transposon mutagenesis to study genes involved in acid-aluminium tolerance (AAT) in B. japonicum. Transposon delivery was carried out through conjugation between B. japonicum strains as recipients and Escherichia coli S17-1 (ë pir) carrying pUTmini- Tn5Km1 as a donor strain. The result showed that frequency of transconjugation was in the range of 6.7 x 10-7 to 7.1 x 10-6 cell per recipients. AAS11 and AAS15 mutants did not grow on Ayanaba media (pH 4.5) containing 50 μM Aluminium. These mutants remained able to form root nodules of Siratro (Macroptilium arthropurpureum) plants revealing genes interrupted by transposon which were responsible for acid-Al tolerance did not correlate with the nodulation genes. Strains tolerant to acid-aluminium and their mutants with a wild type sensitive to acidaluminium were characterized by accumulating phosphate and aluminium absorption. Compared to the wild type acid-aluminium tolerant B. japonicum, there was approximately a three- to eight-times decrease in phosphate accumulation and a five- to seven-times increase in aluminium absorption by these mutants. These results suggest that aluminium and phosphate contents in the bacterial cells may be involved in mechanisms of acid-Al tolerance of B. japonicum grown in acid-aluminium stress conditions.

ANALISIS DATA TERPADU KESEJAHTERAAN SOSIAL DALAM BANTUAN SOSIAL PADA MASYARAKAT MISKIN CIANJUR Nurdiani, Dini
Ar-Rihlah : Jurnal Keuangan dan Perbankan Syariah Vol 3, No 2 (2023): September
Publisher : Universitas Suryakancana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35194/arps.v3i2.3935

ABSTRAKCianjur adalah kabupaten di Jawa Barat dengan populasi yang tinggi, mencapai sekitar 2.506.682 jiwa, di mana 234.500 jiwa di antaranya termasuk dalam kategori masyarakat kurang mampu (data BPS Kabupaten Cianjur tahun 2020). Fokus pembahasan ini adalah strategi kebijakan pemerintah Cianjur dalam penyaluran bantuan sosial kepada masyarakat miskin, menggunakan data skunder seperti penerima bantuan sosial dan Data Terpadu Kesejahteraan Sosial (DTKS) dari tahun 2020-2022. Penelitian ini bersifat studi kasus dengan metode deskriptif analisis, menggambarkan keadaan yang menjadi fokus berdasarkan data yang terkumpul, dengan tujuan mendapatkan informasi teliti dan efisien sebagai pedoman penelitian. Metode deskriptif dilakukan dengan analisis time series, membandingkan rasio keuangan perusahaan dari satu periode ke periode berikutnya. Dengan melihat perkembangan ini, perusahaan dapat merencanakan masa depannya berdasarkan Data Terpadu Kesejahteraan Sosial (DTKS). Populasi dalam penelitian ini mencakup data Akuntabilitas Kinerja DTKS Cianjur dari tahun 2020-2022. Di Dinas Sosial Kota Cianjur, efektivitas pelayanan publik dapat dipengaruhi oleh faktor yang dianggap memiliki pengaruh dominan terhadap tingkat kinerja pelayanan publik. Beberapa faktor tersebut melibatkan kurangnya pemahaman masyarakat terhadap prosedur penerimaan bantuan, yang seharusnya terdaftar di DTKS tanpa menunggu waktu yang lama. Ketidak sabaran juga muncul karena seringnya terjadi pembaruan sistem, yang menyebabkan beberapa Nomor Induk Keluarga tidak terdaftar atau orang yang sudah bekerja di sektor swasta tidak tercatat. Dalam melakukan verifikasi dan validasi data DTKS, disarankan untuk Dinas Sosial Kota Cianjur menambah staf di bagian pengelola data DTKS, mengingat banyaknya data yang perlu diperbaiki. Peningkatan koordinasi dengan Dinas Kependudukan dan Catatan Sipil juga diperlukan untuk perbaikan data masyarakat atau sosialisasi kepada masyarakat agar segera memperbarui data seperti NIK, nama, dan alamat untuk semua anggota keluarga. ABSTRACTCianjur, a regency in West Java, faces high population density with approximately 2,506,682 inhabitants, of which 234,500 are categorized as economically disadvantaged (BPS Cianjur Regency data in 2020). This discussion focuses on the government of Cianjur's policy strategies in distributing social assistance to the impoverished community, utilizing secondary data such as recipients of social aid and Integrated Social Welfare Data (DTKS) from the period 2020-2022. The research adopts a case study approach and employs a descriptive analytical method to depict the focal points based on the gathered data. The objective is to obtain comprehensive and efficient information that can serve as a guide for the research. The descriptive method includes time series analysis, comparing financial ratios from one period to another. This approach allows for an examination of the development, enabling the formulation of future plans based on the Data Terpadu Kesejahteraan Sosial (DTKS). The population for this study comprises the Accountability Performance Data of DTKS in Cianjur from 2020 to 2022. In the Department of Social Affairs in Cianjur, the effectiveness of public services can be influenced by factors deemed to have a dominant impact on the public service performance level. These factors include the community's lack of understanding of the procedures to receive aid, resulting in delayed registration in DTKS. Impatience arises due to frequent system updates, leading to some family identification numbers not being registered or individuals with private sector employment not being accounted for. To enhance the verification and validation of DTKS data, it is recommended for the Cianjur Department of Social Affairs to increase the number of staff managing DTKS data. This is essential due to the substantial volume of data requiring correction. Additionally, improving coordination with the Department of Population and Civil Registration is crucial for data rectification or conducting public awareness campaigns to promptly update information such as NIK, names, and addresses for all family members.

PENGARUH BIAYA OPERASIONAL PENDAPATAN OPERASIONAL (BOPO) TERHADAP RETURN ON ASSET (ROA)PADA BPRS HIK PARAHYANGAN TAHUN 2020–2023 Nurdiani, Dini; Leni, Leni
Ar-Rihlah : Jurnal Keuangan dan Perbankan Syariah Vol 4, No 1 (2024): Maret
Publisher : Universitas Suryakancana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35194/arps.v4i1.4677

Penelitian ini dilatarbelakangi oleh adanya fenomena bahwa Biaya Operasional Pendapatan Operasional (BOPO) dan Return On Asset (ROA) pada BPRS HIK Parahyangan tahu 2020-2023 mengalami kondisi yang fluktuasi. Tujuan penelitian ini adalah untuk menilai seberapa besar pengaruh Biaya Operasional PendapatanOperasional (BOPO) terhadap Return On Asset (ROA) pada BPRS HIK Parahyangan tahun 2020 – 2023. Metode Penelitian ini yang digunakan adalah analisis deskriptif asosiatif dengan pengambilan data populasi secara sekunder pada laporan keuangan BPRS HIK Parahyangan. Adapun sampelnya adalah data pertahun Biaya Operasional Pendapatan Operasional (BOPO) dan Return On Asset (ROA) pada BPRS HIK Parahyangan tahun 2017-2022 yaitu sebanyak 12 data. Hasil penelitian ini menyimpulkan bahwa BOPO berpengaruh negatif dan signifikan terhadap ROA pada BPRS HIK Parahyangan. Hasil Koefisien Determinasi yang menunjukan bahwa pengaruh variabel BOPO (X) terhadap ROA (Y) sebesar 97,6%, sedangkan sisanya 2,4% dijelaskan oleh variabel lain yang tidak diteliti dalam penelitian ini. This research was motivated by the phenomenon that Operational Costs, Operational Income (BOPO) and Return On Assets (ROA) at BPRS HIK Parahyangan, 2020-2023 experienced fluctuating conditions. The aim of this research is to assess how much influence Operational Costs and Operational Income (BOPO) have on Return On Assets (ROA) at BPRS HIK Parahyangan in 2020-2023. The research method used is associative descriptive analysis with secondary population data collection in the financial reports of BPRS HIK Parahyangan. The sample is annual data on Operational Costs, Operational Income (BOPO) and Return On Assets (ROA) at BPRS HIK Parahyangan for 2017-2022, namely twelve data. The results of this research conclude that BOPO has a negative and significant effect on ROA at BPRS HIK Parahyangan. the results of the Coefficient of Determination which shows that the influence of the BOPO (X) variable on ROA is 97.6%, while the remaining 2.4% is explained by other variables not examined in this research.

Influence of Co-feeding Methanol-sorbitol Ratio on Production of Human Insulin Precursor Expressed by Mut+ Pichia pastoris Puspitasari, Dian Japany; Mahsunah, Anis Herliyati; Nurdiani, Dini; Astuti, Rika Indri; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.581-588

An increasing number of diabetic patients and the demand for insulin encourage the development of recombinant insulin production on a large scale. Human insulin precursor (HIP) expressed by Mut+ Pichia pastoris using methanol as an inducer was developed. However, methanol above 5% (w/v) or 1.56 M is toxic for the host. Sorbitol was introduced as a co-substrate with methanol. To our knowledge, the study of methanol/sorbitol co-feeding on human insulin precursor (HIP) expression by Mut+ Pichia pastoris in a bioreactor has yet to be reported. This study aimed to investigate the influence of the methanol-sorbitol co-feeding ratio on the expression of HIP expressed by P. pastoris X33 Mut+. The study was conducted by comparing the cultivation of P. pastoris/pD902-IP Mut+ in a 10-liter bioreactor under three conditions: feeding 100% methanol, mass ratio of MeOH:sorbitol 12:1 and 3:1. The oxygen consumption of methanol/sorbitol is less than the methanol feeding. The mass ratio of MeOH:sorbitol 12:1 produced the highest HIP titer (1326.5 mg/L), 1.5 times higher than methanol feeding, the lowest specific growth rate, but the highest specific productivity at the induction phase. MeOH:sorbitol mass ratio 3:1 produced the highest dry cell weight (DCW) amount (96 g/L). These results suggested that an appropriate ratio of sorbitol-methanol can be a choice to replace methanol feeding in a Mut+ P. pastoris.

A “Klepon Si Centing” Training of breastfeeding counselor cadres to prevent stunting in cimahi city Hernawati, Erni; Nurdiani, Dini; Cornelia Rahandity, Dionisia; Febriyanti Hartini, Eka; Hijriah Handayani, Elis; Ratna Sari Priatna, Fuji; Handayani, Idah; Apriliani, Irrena; Maelani, Leni; Marlina, Marlina; Anna Umilla, Rizqa; Masriyah, Siti; Suminar, Suminar; Prihatin Ningrum, Yuan
Journal Pengabdian Masyarakat Politeknik Sandi Karsa Vol 4 No 2 (2025): Abdimas Polsaka: Jurnal Pengabdian Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat,Politeknik Sandi Karsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35816/abdimaspolsaka.v4i2.93

Stunting is a chronic nutritional problem that is still a public health challenge in Indonesia, including in Cimahi City, which has a stunting prevalence of 9.4%. One of the efforts to prevent stunting is increasing exclusive breastfeeding coverage, which is still below the national target. This community service activity aims to increase the capacity of health cadres in breastfeeding counselling knowledge and skills through intensive community-based training. The program will be carried out in March 2025 in RW 06 Melong Village, the working area of the Central Melong Health Centre, Cimahi City, with eight cadre participants. Evaluation was carried out through pre-test and post-test using a questionnaire consisting of 20 questions that had been tested for validity and reliability, as well as participatory observation of changes in attitudes and skills of cadres. The results showed an increase in the average knowledge score of 33.1 points with a significant paired t-test result (p = 0.001). Qualitatively, participants showed increased self-confidence, skills in practising lactation techniques and oxytocin massage, and initiatives to form breastfeeding support groups to sustain education. This program contributes to cadre empowerment and is a potential strategy to support the acceleration of stunting reduction at the community level.

EFEKTIVITAS PENGGUNAAN APLIKASI FINANCIAL MANAGEMENT INFORMATION SYSTEM DALAM MENINGKATKAN KUALITAS LAPORAN KEUANGAN PADA DINAS PUTR KABUPATEN CIANJUR Oktaviani, Yugianti; Nurdiani, Dini
Jurnal Akuntansi Vol 3, No 1 (2023): September
Publisher : Universitas Suryakancana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35194/ajaki.v3i1.4659

Penelitian ini dilatarbelakangi adanya Undang-undang Nomor 32 Tahun 2004 tentang Pemerintah Daerah menyatakan bahwa pemerintah daerah diberi kewenangan mengatur urusan keuangannya dan Undang-undang Nomor 1 Tahun 2004 tentang Perbendaharaan Negara, pemerintah diwajibkan menerapkan basis akuntansi akrual secara penuh atas pengakuan dan pengukuran pendapatan mendorong pemerintah daerah untuk meningkatkan pengelolaan keuangannya. Aplikasi Financial Management Information System (FMIS) yang dirancang oleh Badan Pengawas Keuangan dan Pembangunan (BPKP). Yang dimana aplikasi FMIS ini merupakan suatu sistem informasi yang dibangun, dikembangkan dan digunakan untuk melakukan proses pengelolaan keuangan daerah mulai dari perencanaan sampai dengan pelaporan dan akuntabilitas kinerja. Penerapan aplikasi FMIS ini didasarkan pada Peraturan Kementerian Dalam Negeri (Permendagri) Nomor 90 tahun 2019 tentang klasifikasi, kodefikasi, dan Nomenklatur Perencanaan dan Pembangunan Keuangan Daerah dan Permendagri. Penelitian ini bertujuan untuk mengetahui efektivitas penggunaan aplikasi FMIS pada Dinas Pekerjaan Umum dan Tata Ruang (PUTR) dan untuk mengetahui sejauh mana FMIS berperan dalam peningkatan kualitas laporan keuangan pada Dinas PUTR. Metode penelitian yang digunakan peneliti adalah metode penelitian kualitatif deskriptif dengan pendekatan fenomenologi. Teknik pengumpulan data adalah berupa penelitian sumber dan observasi yang terdiri atas wawancara dan dokumentasi. Hasil penelitian menyimpulkan bahwa, sistem aplikasi FMIS penggunaan aplikasi FMIS ini kurang efektif tetapi masih banyak kendala yang sedang dihadapi salah satunya yaitu sistem eror, ketidak kelancaran dalam penginputan pajak. Walaupun pelaksanaannya sudah sesuai dengan SOP. Proses penggunaan aplikasi FMIS di Dinas PUTR bahwa peningkatan kualitas laporan keuangan harus menyesuaikan dengan kebutuhannya menyatakan bukti laporan SP2D bahwa bukti SP2D yang sudah dilaksanakan dengan sah. This research is Law Number 32 of 2004 concerning Regional Government states that regional governments are given the authority to regulate their financial affairs and Law Number 1 of 2004 concerning State Treasury, the government is required to implement a full accrual basis of accounting for the recognition and measurement of income encouraging regional governments to improve management. his finances. The FMIS application was designed by the Financial and Development Supervisory Agency (BPKP). The FMIS application is an information system that is built, developed and used to carry out regional financial management processes starting from planning to reporting and performance accountability. The implementation of the FMIS application is based on the Ministry of Home Affairs Regulation (Permendagri) Number 90 of 2019 concerning classification, codification and nomenclature of Regional Financial Planning and Development and the Minister of Home Affairs Regulation. This research aims to determine the effectiveness of using the FMIS application at the PUTR Service and to determine the extent to which FMIS plays a role in improving the quality of financial reports at the PUTR Service. The research method used by researchers is a descriptive qualitative research method with a phenomenological approach. Data collection techniques are in the form of source research and observation consisting of interviews and documentation. The results of the research concluded that, using the FMIS application system, the use of the FMIS application is less effective but there are still many obstacles being faced, one of which is system errors, irregularities in tax input. Even though the implementation is in accordance with the SOP. The process of using the FMIS application in the PUTR Service means that improving the quality of financial reports must be adjusted to their needs, stating that the SP2D report shows proof that the SP2D has been implemented legally.

PENGARUH KUALITAS SUMBER DAYA MANUSIA DAN PENGENDALIAN INTERNAL TERHADAP EFEKTIVITAS PENERAPAN SISTEM FINANCIAL MANAGEMENT INFORMATION SYSTEM DI LINGKUNGAN KABUPATEN CIANJUR Amalia, Nurulita Fauzzi; Nurdiani, Dini
Jurnal Akuntansi Vol 3, No 2 (2024): Maret
Publisher : Universitas Suryakancana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35194/ajaki.v3i2.4660

Setiap Dinas di Kabupaten Cianjur menggunakan aplikasi FMIS untuk mengelola pelaporan keuangan. Penggunaan Aplikasi Financial Management Information System (FMIS) masih memiliki beberapa kendala yang dihadapi yaitu, aplikasi FMIS masih baru dan membutuhkan Sumber Daya Manusia yang berkompeten dan membutuhkan waktu yang lama dalam memahami pemakaian Aplikasi FMIS. Penelitian ini bertujuan untuk menjelaskan keterpengaruhan antara SDM dan Pengendalian Internal terhadap efektivitas penerapan FMIS baik secara parsial maupun simultan. Penelitian ini menggunakan pendekatan kuantitatif dengan menggunakan metode deskriptif dan asosiatif. Populasi dan sampling dalam penelitian ini adalah seluruh kepala bagian keuangan di 25 OPD Kabupaten Cianjur sebanyak 25 orang yang menggunakan aplikasi FMIS.teknik pengambilan sampel pada penelitian ini menggunakan total sampling. Teknik analisa data menggunakan analisis regresi linier berganda. Berdasarkan hasil uji hipotesis menggunakan pengujian parsial ( uji t) untuk variabel kualitas SDM diperoleh nilai t hitung lebih besar dari pada t tabel (2.441 > 2.073) dengan nilai signifikan (0,023 < 0,05) sedangkan variabel pengendalian internal diperoleh nilai t hitung lebih besar dari pada t tabel ( 3.315 > 2.073) dengan nilai signifikan ( 0,003 < 0,05) dan hasil uji simultan (uji F) signifikansinya adalah 0,001 < 0,05,artinya terdapat pengaruh antara kualitas SDM dan pengendalian intenal terhadap efektivitas penerapan sistem FMIS.. hasil uji koefisien determinasi (R2) menunjukkan nilai 92,3% sisanya 7,7% dipengaruhi variabel lain, yang tidak diteliti dalam penelitian ini. Every Department in Cianjur Regency uses the FMIS application to manage financial reporting. The use of the Financial Management Information System (FMIS) application still has several obstacles faced, namely, the FMIS application is still new and requires competent human resources and requires a long time to understand the use of the FMIS application. This research aims to explain the influence between HR and Internal Control on the effectiveness of implementing FMI) both partially and simultaneously. This research uses a quantitative approach using descriptive and associative methods. The population and sampling in this study were all heads of finance in 25 OPDs in Cianjur Regency, 25 people who used the FMIS application. The sampling technique in this study used total sampling. The data analysis technique uses multiple linear regression analysis. Based on the results of hypothesis testing using partial testing (t test) for the human resource quality variable, the calculated t value was greater than the t table (2,441 > 2,073) with a significant value (0.023 < 0.05) while the internal control variable obtained a greater t value. from the t table (3,315 > 2,073) with a significant value (0.003 < 0.05) and the results of the simultaneous test (F test) the significance is 0.001 < 0.05, meaning that there is an influence between the quality of human resources and internal control on the effectiveness of implementing the FMIS system.. The results of the coefficient of determination test (R2) show a value of 92.3%, the remaining 7.7% is influenced by other variables, which were not examined in this study.

ANALISIS FAKTOR IMPLEMENTASI PENYUSUNAN LAPORAN KEUANGAN PADA UMKM BERDASARKAN SAK EMKM (Studi Kasus UMKM Manisan Mekar Sari Cianjur) Rosdiana, Dian; Nurdiani, Dini; Muslim, Buchori
Jurnal Akuntansi Vol 3, No 1 (2023): September
Publisher : Universitas Suryakancana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35194/ajaki.v3i1.4655

Penelitian ini bertujuan untuk mengetahui factor implementasi penyusunan laporan keuangan berdasrkan Standar Akuntansi Keuangan Entitas Mikro, Kecil, dan Menengah (SAK EMKM) yang telah ditetapkan dan mempermudah UMKM menyusun dan menyajikan laporan keuangan yang lebih sederhana dan mudah dipahami. Penelitian ini dilakukan pada UMKM Manisan Mekar Sari Cianjur, sebuah usaha yang membuat manisan dari aneka buah-buahan yang berdiri pada tahun 2011. Adapun penelitian ini merupakan penelitian lapangan yang menggunakan metode kualitatif deskriptif sumber data yang digunakan adalah data primer dan sekunder dengan Teknik koding, pengelompokkan, dan deskripsi pembahasan untuk dapat memperoleh narasi hasil analisispenelitian. Hasil penelitian ini menunjukan bahwa, system pencatatan keuangan UMKM Manisan Mekar Sari Cianjur dicatat secara manua dan masih sederhana, dikarekana pemilik belum memahami penyusunan laporan keuangan yang sesuai dengan standar UMKM serta belum adanya sosialisasi yang di terima terkait dengan SAK EMKM yang ditetapkan. Penelitian ini juga di harapkan dapat memberikan informasi bagi pemilik UMKM Manisan Mekar Sari Cianjur dalam mengimplementasikan penyusunan laporan keuangan berdasarkan SAK EMKM This study aims to determine the implementation factors for the preparation of financial statements based on the Financial Accounting Standards for Micro, Small, and Medium Entities (SAK EMKM) that have been established and make it easier for MSMEs to compile and present simpler and easier to understand financial statements. This research was conducted on MSMEs Mekar Sari Cianjur, a business that makes sweets from various fruits that was established in 2011. This research is a field research which uses the qualitative descriptive method, the data sources used are primary and secondary data with coding techniques, grouping, and discussion descriptions to be able to obtain a narrative of the results of the research analysis. The results of this study show that, the financial recording system of Manisan Mekar Sari Cianjur MSMEs is recorded humanly and is still simple, because the owner has not understood the preparation of financial statements in accordance with MSME standards and there has been no socialization received related to the established SAK EMKM. This research is also expected to provide information for MSME owners of Manisan Mekar Sari Cianjur in implementing the preparation of financial statements based on SAK EMKM

Isolation and Characterization of OsNAC6 cDNA from Rice (Oryza sativa L.) cv. Nipponbare, Batutegi, and Rojolele Rachmat, Agus; Nugroho, Satya; Nurdiani, Dini; Swastika, Maria; Sukma, Dewi; Aswidinnoor, Hajrial; Sudarsono, Sudarsono
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Transcription factors have an important function in regulating gene expression and plant responses to stresses. The ERF, bZIP, WRKY, MYB, and NAC are stress inducible transcription factors. The OsNAC6 is a member of the NAC transcription factor family in rice and its expression is induced by abiotic stresses, wounding and blast disease. Characterization of OsNAC6 gene sequences would give a better understanding on how OsNAC gene functions biologically. The objectives of this research are to isolate the OsNAC6 cDNA from Nipponbare, Batutegi, and Rojolele cultivars, to characterize their DNA sequences, and to compare their sequences to other NAC genes from other plants available in GenBank DNA databases. Isolated cDNA and sequencing of the fragments resulted in a 912 bp DNA sequences. Translation of the sequences yielded a protein consisted of 303 amino acid residue. Blast analysis of amino acid sequences indicated identity of isolated cDNA from three Indonesian rice cultivars are the OsNAC6 gene. Deduced amino acid residues from amplified cDNAs of Nipponbare, Batutegi, and Rojolele cultivars shared 100% sequence identities to rice OsNAC6 (Acc. # BAA89800), 71-100% sequence identity to a number of OsNAC protein from Oryza sativa and 63-83% sequence identity to NAC protein from other plants.

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