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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Program Kreativitas Mahasiswa - Penelitian Proceedings of the International Conference on Applied Science and Health Acta Biochimica Indonesiana Journal of Community Empowerment for Health
Abrory Agus Cahya Pramana
Department Of Biochemistry, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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PENGEMBANGAN MARKA MOLEKULAR UNTUK KARAKTERISASI VARIETAS ANGGREK TANAH UNGGUL (SPATHOGLOTTIS) HASIL POLIPLOIDISASI DENGAN KOLKISIN Setiawan, Agus; Shochicha, Anahtadiya Nurfa; Cahya Pramana, Abrory Agus; Restiyanti, Restiyanti; Daryono, Budi Setiadi
Program Kreativitas Mahasiswa - Penelitian PKM-P 2013
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.957 KB)

Abstract

Characterization of Spathoglottis has not been observed yet especially in determination of genetic relationship and identification of colchisine-induced polyploid orchid. The aim of this research was to study about characterization of fingerprinting molecular mark in DNA Barcode profiling of Polyploid Anggrek Tanah (Spatholgottis sp.) and fenetic relationship of polyploid orchid with superior hybrid soil orchid (Spatholgottis sp.). The method of this research is collecting the orchid, germinating orchid seed, colchisine-induced PLB orchid, making simply buffer DNA isolation, genome DNA isolation, quantitative test of genome DNA, qualitative test of genome DNA, liquidity DNA genom, liquidity RAPD primer, PCR Random Amplified Polimorphism DNA (RAPD) of Orchid DNA, electrophoresis of PCR-RAPD, polymorphism RAPD , Dendogram RAPD analysis, dan creating Orchid DNA barcode. Based on the result known that RAPD molecular method could be used in detection of polyploid Spathoglottis sp. with OPAW11 primer. Electroforegram could be made as DNA bar-coding for Spathoglottis sp. that also could be used to to trace the origin orchids from Indonesia.Keywords: Spathoglottis, RAPD, bar-code DNA, colchisine
POTENTIAL CHEMOPREVENTIVE AGENT: STUDY OF APOPTOSIS IN THE EXTRACTS OF SPONGE-ASSOCIATED FUNGI FROM YOGYAKARTA AGAINST CERVICAL CANCER HeLa CELL LINE Ramadhani, Eka; Priyambada, Fajar; Pramana, Abrory Agus Cahya; Subchan, Aditya Nur; Pertiwi, Gian Aditya; Setiawibawa, Raden Aditya Aryandi; Putra, Hendy Eka; Amalia, Nur Rofika Ayu Shinta; Wijayanti, Nastiti
Proceedings of the International Conference on Applied Science and Health No 1 (2017)
Publisher : Proceedings of the International Conference on Applied Science and Health

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Abstract

Background: Cervical cancer is one of the leading-cancers affecting women. Cancer drugs that do not originate from natural ingredient, chemotherapy drugs, have side and resistant effects. Thus study about the natural products treating cancer cells is needed. Secondary metabolites isolated from sponge-associated fungi are expected to have a potency to fight cancer cells. In addition, the production of anticancer compounds from microorganisms has several advantages, including rapid growth and can be manipulated to increase productivity. The isolation and testing cytotoxicity against 3 fungal isolates from Yogyakarta have been done on the previous research. All three isolates have a potential candidate as anticancer drug. Aims: The purpose of this advanced study was studying bioactive compounds induced apoptosis pathway of sponge-associated fungi against cervical cancer HeLa cells. Methods: This study has been carried out for approximately 5 months. The method conducted in this research including the sponge cultivation (covers growth and isolation of secondary metabolites), the mycelium extraction of fungi, the cytotoxicity assay against HeLa cells using MTT Assay and Apoptosis Staining was to see the induction of apoptosis pathway. Results: Based on the research showed that ethyl acetate extract from mycelium is 0.22 grams. The cytotoxicity assay from mycelium extract showed IC50 value of 164 μg/mL against HeLa cell line. Conclusion: The findings is carrying to a possibility to develop the extracts of sponge-associated fungi as candidate of anti-cancer compound. By apoptosis staining, showed the cells coloured green are still alive, and cells undergoing apoptosis have nucleus that appears orange to red. We assuming that the apoptosis was caused by the possibility of peptide compounds that induce apoptosis through the mitochondrial pathway, by increasing the activity of the protein expression of apoptosis, which are Bcl-2 and Bcl-xl. 
Correlation between ratio of Nrf2/Keap1 and catalase gene expression in liver of hyperlipidemic rats after administration of 7-hydroxy-2-(4-hydroxy- 3-methoxyphenyl)-chromen-4-one Prasetyastuti, .; Rubi, Dianandha Septiana; Pramana, Abrory Agus Cahya; Sadewa, Ahmad Hamim
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.702 KB) | DOI: 10.19106/JMedScie/005101201903

Abstract

Hyperlipidemia results in excessive superoxide anion radicals that are the cause ofoxidative stress. Phytochemical compounds can reduce oxidative stress. The aim of thisstudy was to investigate the correlations between ratio of Nrf2/Keap1 and catalase geneexpression in livers of hyperlipidemic rats after administration of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one. Twenty-four Rattus norvegicus rats, aged 8 weeks andweighing an average of 200 g were randomly divided into 6 groups i.e. Group 1 wasnormal group (N), Group 2 was hyperlipidemic rats (HL), Group 3 was hyperlipidemicrats with simvastatin (HL+SV), and Groups 4-6 were hyperlipidemic rats with 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one doses 10 mg (HL+10), 30 mg (HL+30) or 90 mg/200 g BW (HL+90), respectively, administered orally by gavages. At the end ofthe study, the rats were euthanized and the livers were used to analyze the ratio of Nrf2/Keap 1 and catalase gene expression. Nrf2/Keap1 ratio and catalase gene expressionbetween groups were analyzed by Kruskal Wallis test. Spearman’s correlation test wasused to analyze the correlations between Nrf2/Keap1 ratio and catalase gene expression.The administration of 3 different doses of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one on hyperlipidemic rats increased catalase gene expression. There wasno correlation between ratio Nrf2/Keap1 and catalase gene expression. In conclusion,administration of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one can improvecatalase gene expression in hyperlipidemic rats. However, there is no correlation betweenthe ratio of Nrf2/Keap1 gene expression and the catalase gene expression.
The protective effects of red beetroot (Beta vulgaris l.) against oxidative stress in rats induced by high fat and fructose diet Dianandha Septiana Rubi; Abrory Agus Cahya Pramana; Sunarti
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.53

Abstract

Background: One of consequence high-fat and fructose diet is oxidative stress. Consumption of antioxidants from red beetroot may increase antioxidant defense. Objectives: This study aimed to evaluate red beetroot administration on improving antioxidant defense in rats induced high fat and fructose diet. Methods: A total 20 male Wistar rats were divided into 4 groups: 1) normal control group (N), received standard diet; 2) High fat and fructose diet (HF), received high fat and fructose diet (HFFD); 8 weeks induction with HFFD and received 9g red beetroot (BA); and combination of HFFD and 9g of red beetroot from beginning of the study (HFBA). At the end of the study the levels of circulatory oxidized LDL (ox-LDL) were determined using enzyme-linked immunosorbent assay (ELISA) method. Superoxide dismutase 2 (SOD2) and catalase (CAT) gene expressions were determined by quantitative polymerase chain reaction (qPCR) method. Results: Induction HFFD increased the levels of circulatory ox-LDL levels compared to normal control (10.00±0.29 vs 12.69±0.57). Administration of red beetroot for 6 weeks and combination HFFD with red beetroot during the study significantly decreased ox-LDL levels compared to high fat and fructose group (12.69±0.57 vs 9.66±0.46) and (12.69±0.57 vs 8.59±0.18), respectively. The decreased circulatory ox-LDL levels were found negatively correlated with upregulation SOD2 (r=-0.548; P=0.012) and CAT (r=-0.460; P=0.041) gene expression in the liver tissues. Conclusion: Administration of red beetroot may ameliorate oxidative stress in rats induced high-fat and fructose diet through increasing antioxidant defense.
POTENTIAL CHEMOPREVENTIVE AGENT: STUDY OF APOPTOSIS IN THE EXTRACTS OF SPONGE-ASSOCIATED FUNGI FROM YOGYAKARTA AGAINST CERVICAL CANCER HeLa CELL LINE Eka Ramadhani; Fajar Priyambada; Abrory Agus Cahya Pramana; Aditya Nur Subchan; Gian Aditya Pertiwi; Raden Aditya Aryandi Setiawibawa; Hendy Eka Putra; Nur Rofika Ayu Shinta Amalia; Nastiti Wijayanti
Proceedings of the International Conference on Applied Science and Health No. 1 (2017)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Cervical cancer is one of the leading-cancers affecting women. Cancer drugs that do not originate from natural ingredient, chemotherapy drugs, have side and resistant effects. Thus study about the natural products treating cancer cells is needed. Secondary metabolites isolated from sponge-associated fungi are expected to have a potency to fight cancer cells. In addition, the production of anticancer compounds from microorganisms has several advantages, including rapid growth and can be manipulated to increase productivity. The isolation and testing cytotoxicity against 3 fungal isolates from Yogyakarta have been done on the previous research. All three isolates have a potential candidate as anticancer drug. Aims: The purpose of this advanced study was studying bioactive compounds induced apoptosis pathway of sponge-associated fungi against cervical cancer HeLa cells. Methods: This study has been carried out for approximately 5 months. The method conducted in this research including the sponge cultivation (covers growth and isolation of secondary metabolites), the mycelium extraction of fungi, the cytotoxicity assay against HeLa cells using MTT Assay and Apoptosis Staining was to see the induction of apoptosis pathway. Results: Based on the research showed that ethyl acetate extract from mycelium is 0.22 grams. The cytotoxicity assay from mycelium extract showed IC50 value of 164 μg/mL against HeLa cell line. Conclusion: The findings is carrying to a possibility to develop the extracts of sponge-associated fungi as candidate of anti-cancer compound. By apoptosis staining, showed the cells coloured green are still alive, and cells undergoing apoptosis have nucleus that appears orange to red. We assuming that the apoptosis was caused by the possibility of peptide compounds that induce apoptosis through the mitochondrial pathway, by increasing the activity of the protein expression of apoptosis, which are Bcl-2 and Bcl-xl. 
Hypothyroidism and stunting around the Merapi Volcano Pramudji Hastuti; Ahmad Hamim Sadewa; Prasetyastuti -; Sunarti -; Arta Farmawati; Ngadikun -; Dianandha Septiana Rubi; Abrory Agus Cahya Pramana
Journal of Community Empowerment for Health Vol 4, No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.61025

Abstract

Cangkringan is a mountainous area with the possibility of low iodine sources in the soil. Additionally, Cangkringan area is the area nearest to where eruptions of Merapi mountain have occurred, which further could reduce iodine levels in the soil. This study examined the incidence of hypothyroidism due to iodine deficiency and potential links to stunting events on the slopes of Merapi mountain. By using ELISA methods, a total of 97 mothers were tested to detect the triiodothyronine (T3) and thyroid-stimulating hormone (TSH) levels to determine the frequency of hypothyroidism, while 97 children were assessed to check the stunting rates by measuring height compared to age. We found hypothyroidism was 3 (3.1%) out of 97 mothers examined and 30 (30.9%) out of 97 children examined were stunted (18.5% short stature and 12.4% very short stature). There was a significant difference between the mothers’ TSH levels among very short stature and normal stature. We concluded that hypothyroidism in mothers is correlated with stunting children in Cangkringan, Sleman sub-district. Further research is needed to determine the frequency of hypothyroidism and stunting in other slopes of Merapi mountain, where the low-iodine soil composition is potentially related to hypothyroidism and stunting incidence. Thus, further treatment is needed by local health staff and governments to address the negative effects of hypothyroidism and stunting.
POTENTIAL CHEMOPREVENTIVE AGENT: STUDY OF APOPTOSIS IN THE EXTRACTS OF SPONGE-ASSOCIATED FUNGI FROM YOGYAKARTA AGAINST CERVICAL CANCER HeLa CELL LINE Eka Ramadhani; Fajar Priyambada; Abrory Agus Cahya Pramana; Aditya Nur Subchan; Gian Aditya Pertiwi; Raden Aditya Aryandi Setiawibawa; Hendy Eka Putra; Nur Rofika Ayu Shinta Amalia; Nastiti Wijayanti
Proceedings of the International Conference on Applied Science and Health No. 1 (2017)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Cervical cancer is one of the leading-cancers affecting women. Cancer drugs that do not originate from natural ingredient, chemotherapy drugs, have side and resistant effects. Thus study about the natural products treating cancer cells is needed. Secondary metabolites isolated from sponge-associated fungi are expected to have a potency to fight cancer cells. In addition, the production of anticancer compounds from microorganisms has several advantages, including rapid growth and can be manipulated to increase productivity. The isolation and testing cytotoxicity against 3 fungal isolates from Yogyakarta have been done on the previous research. All three isolates have a potential candidate as anticancer drug. Aims: The purpose of this advanced study was studying bioactive compounds induced apoptosis pathway of sponge-associated fungi against cervical cancer HeLa cells. Methods: This study has been carried out for approximately 5 months. The method conducted in this research including the sponge cultivation (covers growth and isolation of secondary metabolites), the mycelium extraction of fungi, the cytotoxicity assay against HeLa cells using MTT Assay and Apoptosis Staining was to see the induction of apoptosis pathway. Results: Based on the research showed that ethyl acetate extract from mycelium is 0.22 grams. The cytotoxicity assay from mycelium extract showed IC50 value of 164 μg/mL against HeLa cell line. Conclusion: The findings is carrying to a possibility to develop the extracts of sponge-associated fungi as candidate of anti-cancer compound. By apoptosis staining, showed the cells coloured green are still alive, and cells undergoing apoptosis have nucleus that appears orange to red. We assuming that the apoptosis was caused by the possibility of peptide compounds that induce apoptosis through the mitochondrial pathway, by increasing the activity of the protein expression of apoptosis, whichare Bcl-2 and Bcl-xl.
Co-Authors Aditya Nur Subchan Aditya Nur Subchan Agus Setiawan Ahmad Hamim Sadewa Amalia, Nur Rofika Ayu Shinta Anahtadiya Nurfa Shochicha Arta Farmawati BUDI SETIADI DARYONO Dianandha Septiana Rubi Eka Ramadhani Eka Ramadhani Fajar Priyambada Fajar Priyambada Gian Aditya Pertiwi Gian Aditya Pertiwi Hendy Eka Putra Hendy Eka Putra Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ngadikun - Nur Rofika Ayu Shinta Amalia Nur Rofika Ayu Shinta Amalia Pertiwi, Gian Aditya Pramudji Hastuti Prasetyastuti Prasetyastuti, . Priyambada, Fajar Putra, Hendy Eka Raden Aditya Aryandi Setiawibawa Raden Aditya Aryandi Setiawibawa Ramadhani, Eka Restiyanti Restiyanti Setiawibawa, Raden Aditya Aryandi Subchan, Aditya Nur Sunarti Sunarti Sunarti