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Characteristics of Purple Sweet Potato Yogurt and Its Effect on Lipid Profiles of Sprague Dawley Rats Fed with High Fat Diet Lily Arsanti Lestari; Qisthira Swasti Amirina; Dina Septari Anindyah; Ainun Nisa; Annisa Nurul Huda; Widiyani Indrianingrum; Prasetyastuti; Nurliyani
Journal of Food and Pharmaceutical Sciences Vol 8, No 2 (2020): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.679

The objectives of this study were to evaluate the effect of purple sweet potato (PSP) yogurt on thelipid profile of Sprague Dawley rats. The PSP yogurt was made from fresh PSP (F-PSP) and its powder (P-PSP).The viscosity, pH, total titratable acid, proximate, antioxidant activity, anthocyanin content, and total lacticacid bacteria (LAB) between those yogurts were compared. Selected yogurt was given to Sprague Dawley ratsfor 4 weeks. Serum lipid profiles were determined after 2 and 4 weeks of treatment. F-PSP yogurt has betterphysical, chemical and microbiological properties compared to P-PSP yogurt. The serum cholesterol andtriglyceride levels in F-PSP yogurt group was not significantly different compared to control group (P>0.05).However, the reduction of serum LDL cholesterol in F-PSP yogurt group was significantly higher than controlgroup. The serum HDL cholesterol in the yogurt group decreased after 2 weeks of treatment, but it increasedafter 4 weeks of treatment. F-PSP yogurt could be used as an alternative functional food that could lower theblood cholesterol level because of the ability to reduce the LDL cholesterol level.

Effects of quercetin on the nicotine-induced oxidative status in male Wistar rats: study on c-reactive protein (CRP) and malondialdehyde (MDA) concentrations Deri Riskiyanti Tallo Manafe; Denny Agustiningsih; . Prasetyastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 2 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTNicotine can cause atherosclerosis by activating nuclear factor-kB (NF-kB) pathway lead to induce proinflammatory cytokines release as C-reactive protein (CRP) main regulators. The increase of CRP can induce reactive oxygen species (ROS) and increase of malondialdehyde (MDA). Quercetin has been proven to have antiinflammatory and antioxidant effects. This study was conducted to evaluate effect of quercetin on serum CRP and MDA concentrations in rats induced by nicotine. This was a true experimental study with post test only control group design. Thirty six of male Wistar rats were divided into six groups. Group I as normal control received 1 mL/kg BW of NaCl 0.9% solution. Group II as negative control received 2 mg/kg BW of nicotine and Group III as positive control received 2 mg/kg BW of nicotine and atorvastatin at dose of 5 mg/kg BW. Group IV-VI as treatment groups received 2 mg/kg BW of nicotine and quercetin at dose of 25; 50 or 100 mg/kg BW, respectively. Nicotine was given subcutaneously whereas atorvastatin and quercetin were given orally once per day for 28 days, consecutively. Serum CRP and MDA concentrations were measured using Rat hs-CRP ELISA kit and TBARS assay kit, respectively. Data were analyzed using analysis of variance (ANOVA) continued using LSD post-hoc test. The results showed that quercetin reduced serum CRP and MDA concentrations in dose dependent manner. Serum CRP concentration on Group V (173.39 ± 34.85 ng/mL) and Group VI (114.15 ± 43.62 ng/mL) were significantly lower than that Group II (244.77 ± 37.95 ng/mL) (p<0.05). Furthermore, serum MDA concentration on Group IV (5.95 ± 0.11 mmol/mL), Group V (3.93 ± 0.09 mmol/mL) and Group VI (2.14 ± 0.09 mmol/mL) were significantly lower than that Group II (7.29 ± 0.06 mmol/mL) (p<0.05). In conclusion, quercetin reduces the nicotine-induced oxidative status in rats.

Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats Rahmah Dara Ayunda; Prasetyastuti Prasetyastuti; Pramudji Hastuti
Indonesian Journal of Pharmacy Vol 30 No 3, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.

Hubungan timbal dan krom pada pemakaian pewarna batik dengan kadar hemoglobin dan packed cell volume pada pengrajin batik di Kecamatan Lendah Kulon Progo Pramudji Hastuti; Sunarti Sunarti; Prasetyastuti Prasetyastuti; Ngadikun Ngadikun; Tasmini Tasmini; Dianandha Septiana Rubi; Sri Sutarni; Indrasari Kusuma Harahap; Kusumo Dananjoyo; Suhartini Suhartini; Ida Bagus Gd. Surya Putra Pidada; Hendro Widagdo; Martiana Suciningtyas
Journal of Community Empowerment for Health Vol 1, No 1 (2018)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

ABSTRAK Cara membatik di Indonesia mulai banyak beralih dari pemakaian bahan alami menjadi bahan cat sintesis. Cat sintetis yang digunakan sering kali mengandung logam berat di antaranya timbal (Pb) dan kromium (Cr). Pemakaian logam berat dalam waktu lama akan mengganggu kesehatan. Penelitian ini dilakukan untuk melihat hubungan kadar logam Pb dan Cr dalam darah dan limbah dengan kadar hemoglobin (Hb) dan packed cell volume (PCV) serta mengetahui prevalensi gejala toksisitas yang dirasakan pada pekerja di tiga sentra batik di Kecamatan Lendah Kulon Progo. Sebanyak 76 pegawai di tiga sentra batik terdiri atas 37 orang dari sentra SB, 20 orang dari FA dan 19 orang dari SA ditentukan kadar Hb, PCV, kadar Pb dan Cr-nya dengan metode inductively coupled plasma (ICP). Air limbah lingkungan dari ketiga lokasi sentra batik diambil dan diperiksa kadar Pb dan Cr-nya. Hasil antara ketiga lokasi diujinormalitasnya dan dianalisis dengan analisis varian atau Kruskal-Wallis. Hubungan antara kadar Pb dan Cr baik darah maupun limbah dengan kadar logam berat dalam darah dianalisis dengan uji korelasi Pearson atau Spearman. Hasil dinyatakan berbeda bermakna jika p < 0,05. Gejala penyakit yang dialami pekerja yang diperkirakan berhubungan dengan toksisitas logam berat dihitung frekuensinya. Hasil pemeriksaan antropometri, tekanan darah, kadar Hb, PCV dan logam berat dari ketiga lokasi yang diperiksa tidak berbeda bermakna (p > 0,05). Kadar Pb darah dan krom di sentra SB dan FA berbeda bermakna dengan di sentra SA (p < 0,05). Tidak terdapat korelasi yang signifikan secara statistik antara kadar Pb darah, Cr darah, dan Pb limbah dengan kadar Hb dan PCV. Gejala iritasi kulit, pusing, dan nyeri sendi dirasakan oleh 36,8-48,7% responden. Kadar timbal dan krom darah pembatik di sentra SB dan FA lebih tinggi dari pada di sentra SA. Tidak terdapat hubungan bermakna antara toksisitas Pb dan Cr dengan kadar Hb dan PCV. Gejala toksisitas di antara pekerja batik diperkirakan dapat terjadi karena toksisitas logam berat kronis akibat pemakaian cat batik. KATA KUNCI batik; hemoglobin; kromium; sel darah; timbal ABSTRACT The dye used to color batik has changed from natural to synthetic materials in several places in Indonesia. Synthetic paints often contain heavy metals including lead (Pb) and chromium (Cr). Chronic use of heavy metals can affect health. This study was conducted to examine the relationship between blood and waste water levels of Pb and Cr with hemoglobin (Hb) and packed cell volume (PCV), and to determine how many workers felt the toxicity symptoms in three batik centers in Lendah, Kulon Progo. A total of 76 batik center employees, consisted of 37, 20, and 19 people from the SB, FA, and SA centers were recruited. Determination of Hb, PCV, Pb and Cr levels were conducted with the inductively coupled plasma (ICP) method. Waste water from the three batik centers is taken and examined. The data were analyzed by Oneway ANOVA or Kruskal-Wallis. Correlation analyses were performed with Pearson or Spearman correlation test. Results were considered significant if p < 0.05. Disease symptoms experienced by workers that were estimated to be associated with heavy metal toxicity were calculated in frequency. Anthropometrics, blood pressure, Hb levels, PCV and heavy metals levels were not significantly different between centers (p>0.05). Blood Pb and Cr from the SB and FA were significantly different from those at the SA center (p<0.05). There were no statistically significant correlations between blood Pb levels, blood Cr, and Pb waste with hemoglobin and PCV levels. Symptoms of skin irritation, dizziness, and joint pain were felt by 37-49% of respondents. Blood levels of Pb and Cr in the SB and FA centers are higher than in the SA centers. There was no significant correlation between Pb and Cr toxicity with hemoglobin and PCV levels. Symptoms of toxicity among batik workers are thought to occur due to chronic heavy metal toxicity due to the use of synthetic paint.KEYWORDS batik; haemoglobin; chromium; blood cell; plumbum

Kadar glukosa darah dan tekanan darah pada penduduk pedesaan dan perkotaan di Daerah Istimewa Yogyakarta Tasmini Tasmini; Arta Farmawati; Sunarti Sunarti; Pramudji Hastuti; Ahmad Hamim Sadewa; Prasetyastuti Prasetyastuti; Ngadikun Ngadikun
Journal of Community Empowerment for Health Vol 1, No 1 (2018)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

ABSTRAK Sebagian besar penduduk di daerah pedesaan bertaraf ekonomi menengah ke bawah, memiliki keterbatasan akses informasi, dan memiliki mata pencaharian berbeda dibanding penduduk kota. Bantar Kulon merupakan daerah pedesaan dan Kronggahan adalah daerah perkotaan di Yogyakarta. Mengingat terjadinya pergeseran pola penyakit dari penyakit menular ke penyakit tidak menular serta adanya pengaruh lingkungan dan gaya hidup terhadap terjadinya penyakit degeneratif, dilakukan pengkajian mengenai faktor risiko sindroma metabolik di dua daerah tersebut. Penelitian ini dilakukan untuk mengetahui apakah ada perbedaan kadar glukosa darah puasa (GDP) dan tekanan darah (TD), serta keluhan/ penyakit utama pada penduduk di daerah pedesaan dan perkotaan. Sebanyak 71 orang dari Bantar Kulon dan 91 orang dari Kronggahan diperiksa kadar GDP-nya menggunakan GCU Multi-Function Monitoring System (EasyTouch®). Tekanan darah diperiksa dengan sphygmomanometer raksa dan otomatis. Kadar GDP dan TD pada subjek dari kedua lokasi ditampilkan dalam bentuk deskriptif berdasarkan cut-off (GDP: ≥ 100 mg/dL; TD: ≥140/90 mmHg). Uji t atau Mann Whitney U dilakukan untuk mengetahui perbedaaan nilai variabel antara kedua lokasi. Hasil dinyatakan berbeda bermakna jika p < 0,05. Data keluhan penyakit utama ditampilkan secara deskriptif. Tidak ada perbedaan kadar GDP antara desa dan kota (p = 0,385). Persentase subjek yang memiliki GDP ≥ 100 mg/dL lebih banyak di desa dibanding di kota (42,3% vs 26,4%). Persentase hipertensi lebih tinggi di kota dibanding di desa (50,5% vs 33,8%). Berdasarkan wawancara, keluhan/ penyakit utama terbanyak pada kedua wilayah adalah hipertensi sebanyak 23 orang (32,4%) di desa dan 30 orang (33,0%) di kota. Kadar GDP di atas normal lebih banyak ditemukan di desa sedangkan hipertensi lebih banyak ditemukan di kota. Keluhan/ penyakit utama di kedua wilayah adalah hipertensi. KATA KUNCI kadar glukosa darah; penyakit metabolik; hipertensi; pedesaan; perkotaanABSTRACT Most people living in rural areas are from lower to middle income class, have limited access to information, and have different occupations compared to those in urban areas. In Yogyakarta, Bantar Kulon is a rural area, while Kronggahan is an urban area. Currently, the pattern of disease is shifting from infectious diseases to non-communicable diseases with environment and lifestyle factors as determinants. Thus, it is necessary to study the trends of risk factors for metabolic syndrome in both areas. This study aimed to seek the difference of fasting blood glucose (FBG), blood pressure (BP), and major complaints/illness between rural and urban areas. Seventy one people from Bantar Kulon and 91 people from Kronggahan were examined for FBG levels using GCU Multi-Function Monitoring System (EasyTouch®). Blood pressures were checked using sphygmomanometer. Levels of FBG and BP were presented as frequencies based on cut-offs (FBG: ≥ 100mg/dL; BP: ≥ 140/90 mmHg). T-test or Mann-Whitney U test were used to analyze the difference of variables between both areas. Results were significantly different if p < 0.05. Chief complaint ilness data were displayed descriptively. There was no difference in FBG level between rural and urban areas (p = 0.385). The percentage of subjects with FBG ≥ 100 mg / dL was higher in Bantar Kulon than inKronggahan (42.3% vs 26.4%). Percentage of hypertension was higher in urban than rural areas (50.5% vs. 33.8%). Based on interviews, the chief complaint/ illness in both areas was hypertension. The number of subjects who were diagnosed with hypertension were 23 (32.4%) and 30 (33.0%) from Bantar Kulon and Kronggahan respectively. Impaired fasting glucose was more common in rural area while hypertension is more common in urban area. The chief complaint /illness in both regions is hypertension.KEYWORDS blood glucose; metabolic syndrome; hypertension; rural area; urban area

Hypothyroidism and stunting around the Merapi Volcano Pramudji Hastuti; Ahmad Hamim Sadewa; Prasetyastuti -; Sunarti -; Arta Farmawati; Ngadikun -; Dianandha Septiana Rubi; Abrory Agus Cahya Pramana
Journal of Community Empowerment for Health Vol 4, No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.61025

Cangkringan is a mountainous area with the possibility of low iodine sources in the soil. Additionally, Cangkringan area is the area nearest to where eruptions of Merapi mountain have occurred, which further could reduce iodine levels in the soil. This study examined the incidence of hypothyroidism due to iodine deficiency and potential links to stunting events on the slopes of Merapi mountain. By using ELISA methods, a total of 97 mothers were tested to detect the triiodothyronine (T3) and thyroid-stimulating hormone (TSH) levels to determine the frequency of hypothyroidism, while 97 children were assessed to check the stunting rates by measuring height compared to age. We found hypothyroidism was 3 (3.1%) out of 97 mothers examined and 30 (30.9%) out of 97 children examined were stunted (18.5% short stature and 12.4% very short stature). There was a significant difference between the mothers’ TSH levels among very short stature and normal stature. We concluded that hypothyroidism in mothers is correlated with stunting children in Cangkringan, Sleman sub-district. Further research is needed to determine the frequency of hypothyroidism and stunting in other slopes of Merapi mountain, where the low-iodine soil composition is potentially related to hypothyroidism and stunting incidence. Thus, further treatment is needed by local health staff and governments to address the negative effects of hypothyroidism and stunting.

Effects of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chroman-4-one on serum levels of antioxidant enzymes in hyperlipidemic rats . Prasetyastuti; Noviyanty Indjar Gama
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 55, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005502202301

Hyperlipidemia triggers oxidative stress caused by an imbalance between oxidant and antioxidant levels due to the excess production of reactive oxygen species (ROS). The increase of ROS can decrease antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). 7-OH-2-(4-OH-3-Methoxyphenyl)-chroman-4-one is exogenous antioxidants isolated from mahogany seeds (Swietenia macrophylla King). This study aimed to evaluate the effects of the 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one on serum levels of SOD, CAT, and GPx in hyperglycemic rats. Thirty-six male Wistar rats (Rattus norvegicus) were divided into the following six groups: (N) normal group, (HL) hyperlipidemia group, (P) hyperlipidemia group with simvastatin, F10, F30, and F90 hyperlipidemia group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one 10, 30 and 90 mg/200g body weight (BW), respectively. Hyperlipidemia was induced by feed enriched with cholesterol and cholic acid. Treatments were administered orally by gavages. After 4 weeks of treatments, blood sample was drawn and serum levels of SOD, CAT, and GPx enzymes were analyzed using a spectrophotometric method. Serum levels of SOD, CAT, and GPx in hyperlipidemic rats treated with 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chroman-4-one at dose 10, 30 and 90 mg/200g BW were higher than HL group. In addition, no significantly different on serum SOD and CAT between group F90 and group P was observed (p>0.05)

Characteristics of Purple Sweet Potato Yogurt and Its Effect on Lipid Profiles of Sprague Dawley Rats Fed with High Fat Diet Lily Arsanti Lestari; Qisthira Swasti Amirina; Dina Septari Anindyah; Ainun Nisa; Annisa Nurul Huda; Widiyani Indrianingrum; Prasetyastuti; Nurliyani
Journal of Food and Pharmaceutical Sciences Vol 8, No 2 (2020): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.679

The objectives of this study were to evaluate the effect of purple sweet potato (PSP) yogurt on thelipid profile of Sprague Dawley rats. The PSP yogurt was made from fresh PSP (F-PSP) and its powder (P-PSP).The viscosity, pH, total titratable acid, proximate, antioxidant activity, anthocyanin content, and total lacticacid bacteria (LAB) between those yogurts were compared. Selected yogurt was given to Sprague Dawley ratsfor 4 weeks. Serum lipid profiles were determined after 2 and 4 weeks of treatment. F-PSP yogurt has betterphysical, chemical and microbiological properties compared to P-PSP yogurt. The serum cholesterol andtriglyceride levels in F-PSP yogurt group was not significantly different compared to control group (P>0.05).However, the reduction of serum LDL cholesterol in F-PSP yogurt group was significantly higher than controlgroup. The serum HDL cholesterol in the yogurt group decreased after 2 weeks of treatment, but it increasedafter 4 weeks of treatment. F-PSP yogurt could be used as an alternative functional food that could lower theblood cholesterol level because of the ability to reduce the LDL cholesterol level.

Pengenalan Nutrigenomik Pada Guru SMA Biologi dan Kimia di Kota Yogyakarta Melalui Program Kemitraan Masyarakat (PKM) Arta Farmawati; Sunarti Sunarti; Dianandha Septiana Rubi; Ahmad Hamim Sadewa; Prasetyastuti Prasetyastuti; Ngadikun Ngadikun
SWARNA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2024): SWARNA: Jurnal Pengabdian Kepada Masyarakat, Februari 2024
Publisher : LPPM Sekolah Tinggi Ilmu Ekonomi 45 Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/swarna.v3i2.1177

Perkembangan ilmu pengetahuan dan teknologi berdampak pada pengembangan ilmu nutrisi berbasis molekuler diantaranya adalah nutrigenomik. Nutrigenomik merupakan ilmu yang mempelajari bagaimana nutrisi mempengaruhi ekspresi gen. Gen membawa deoxyribose nucleic acid (DNA) yang berisi informasi genetik yang menentukan sintesis protein, dimana protein tersebut berperan dalam berbagai proses biologis, sehingga sangat mempengaruhi kesehatan. Proses biologis merupakan proses reaksi kimia dalam tubuh yang sudah dipelajari sejak sekolah menengah pertama (SMP) oleh karena itu, pengenalan ilmu nutrigenomik pada guru SMA diharapkan dapat meningkatkan pengetahuan terhadap ilmu nutrigenomik pada guru, sehingga dapat meningkatkan kualitas siswa maupun guru sendiri. Upaya ini kami wujudkan dalam bentuk pengabdian masyarakat yang bekerja sama dengan Musyawarah Guru Mata Pelajaran (MGMP) Biologi dan Kimia Kota Yogyakarta. Kegiatan pelatihan yang diikuti 18 guru dilaksanakan dengan beberapa metode pembelajaran seperti ceramah, praktik di laboratorium, tutorial, dan training of trainer (ToT). Berdasarkan hasil evaluasi, program kemitraan masyarakat (PKM) dapat meningkatkan pengetahuan guru-guru mengenai nutrigenomik secara signifikan (43,33 ± 10,01 vs 66,44 ± 13,66) (P<0,05). Hasil ToT menunjukkan tingkat kepercayaan diri guru-guru dalam menyampaikan informasi terkait dengan nutrigenomik menjadi tinggi. Dengan demikian, program ini dapat mengenalkan sekaligus meningkatkan pengetahuan dan rasa percaya diri guru sebagai kader nutrigenomik untuk menyebarluaskan ilmu nutrigenomik kepada siswa maupun masyarakat.

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