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Correlation between ratio of Nrf2/Keap1 and catalase gene expression in liver of hyperlipidemic rats after administration of 7-hydroxy-2-(4-hydroxy- 3-methoxyphenyl)-chromen-4-one Prasetyastuti, .; Rubi, Dianandha Septiana; Pramana, Abrory Agus Cahya; Sadewa, Ahmad Hamim
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Hyperlipidemia results in excessive superoxide anion radicals that are the cause ofoxidative stress. Phytochemical compounds can reduce oxidative stress. The aim of thisstudy was to investigate the correlations between ratio of Nrf2/Keap1 and catalase geneexpression in livers of hyperlipidemic rats after administration of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one. Twenty-four Rattus norvegicus rats, aged 8 weeks andweighing an average of 200 g were randomly divided into 6 groups i.e. Group 1 wasnormal group (N), Group 2 was hyperlipidemic rats (HL), Group 3 was hyperlipidemicrats with simvastatin (HL+SV), and Groups 4-6 were hyperlipidemic rats with 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one doses 10 mg (HL+10), 30 mg (HL+30) or 90 mg/200 g BW (HL+90), respectively, administered orally by gavages. At the end ofthe study, the rats were euthanized and the livers were used to analyze the ratio of Nrf2/Keap 1 and catalase gene expression. Nrf2/Keap1 ratio and catalase gene expressionbetween groups were analyzed by Kruskal Wallis test. Spearman’s correlation test wasused to analyze the correlations between Nrf2/Keap1 ratio and catalase gene expression.The administration of 3 different doses of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one on hyperlipidemic rats increased catalase gene expression. There wasno correlation between ratio Nrf2/Keap1 and catalase gene expression. In conclusion,administration of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one can improvecatalase gene expression in hyperlipidemic rats. However, there is no correlation betweenthe ratio of Nrf2/Keap1 gene expression and the catalase gene expression.

The protective effects of red beetroot (Beta vulgaris l.) against oxidative stress in rats induced by high fat and fructose diet Dianandha Septiana Rubi; Abrory Agus Cahya Pramana; Sunarti
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.53

Background: One of consequence high-fat and fructose diet is oxidative stress. Consumption of antioxidants from red beetroot may increase antioxidant defense. Objectives: This study aimed to evaluate red beetroot administration on improving antioxidant defense in rats induced high fat and fructose diet. Methods: A total 20 male Wistar rats were divided into 4 groups: 1) normal control group (N), received standard diet; 2) High fat and fructose diet (HF), received high fat and fructose diet (HFFD); 8 weeks induction with HFFD and received 9g red beetroot (BA); and combination of HFFD and 9g of red beetroot from beginning of the study (HFBA). At the end of the study the levels of circulatory oxidized LDL (ox-LDL) were determined using enzyme-linked immunosorbent assay (ELISA) method. Superoxide dismutase 2 (SOD2) and catalase (CAT) gene expressions were determined by quantitative polymerase chain reaction (qPCR) method. Results: Induction HFFD increased the levels of circulatory ox-LDL levels compared to normal control (10.00±0.29 vs 12.69±0.57). Administration of red beetroot for 6 weeks and combination HFFD with red beetroot during the study significantly decreased ox-LDL levels compared to high fat and fructose group (12.69±0.57 vs 9.66±0.46) and (12.69±0.57 vs 8.59±0.18), respectively. The decreased circulatory ox-LDL levels were found negatively correlated with upregulation SOD2 (r=-0.548; P=0.012) and CAT (r=-0.460; P=0.041) gene expression in the liver tissues. Conclusion: Administration of red beetroot may ameliorate oxidative stress in rats induced high-fat and fructose diet through increasing antioxidant defense.

Hubungan timbal dan krom pada pemakaian pewarna batik dengan kadar hemoglobin dan packed cell volume pada pengrajin batik di Kecamatan Lendah Kulon Progo Pramudji Hastuti; Sunarti Sunarti; Prasetyastuti Prasetyastuti; Ngadikun Ngadikun; Tasmini Tasmini; Dianandha Septiana Rubi; Sri Sutarni; Indrasari Kusuma Harahap; Kusumo Dananjoyo; Suhartini Suhartini; Ida Bagus Gd. Surya Putra Pidada; Hendro Widagdo; Martiana Suciningtyas
Journal of Community Empowerment for Health Vol 1, No 1 (2018)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

ABSTRAK Cara membatik di Indonesia mulai banyak beralih dari pemakaian bahan alami menjadi bahan cat sintesis. Cat sintetis yang digunakan sering kali mengandung logam berat di antaranya timbal (Pb) dan kromium (Cr). Pemakaian logam berat dalam waktu lama akan mengganggu kesehatan. Penelitian ini dilakukan untuk melihat hubungan kadar logam Pb dan Cr dalam darah dan limbah dengan kadar hemoglobin (Hb) dan packed cell volume (PCV) serta mengetahui prevalensi gejala toksisitas yang dirasakan pada pekerja di tiga sentra batik di Kecamatan Lendah Kulon Progo. Sebanyak 76 pegawai di tiga sentra batik terdiri atas 37 orang dari sentra SB, 20 orang dari FA dan 19 orang dari SA ditentukan kadar Hb, PCV, kadar Pb dan Cr-nya dengan metode inductively coupled plasma (ICP). Air limbah lingkungan dari ketiga lokasi sentra batik diambil dan diperiksa kadar Pb dan Cr-nya. Hasil antara ketiga lokasi diujinormalitasnya dan dianalisis dengan analisis varian atau Kruskal-Wallis. Hubungan antara kadar Pb dan Cr baik darah maupun limbah dengan kadar logam berat dalam darah dianalisis dengan uji korelasi Pearson atau Spearman. Hasil dinyatakan berbeda bermakna jika p < 0,05. Gejala penyakit yang dialami pekerja yang diperkirakan berhubungan dengan toksisitas logam berat dihitung frekuensinya. Hasil pemeriksaan antropometri, tekanan darah, kadar Hb, PCV dan logam berat dari ketiga lokasi yang diperiksa tidak berbeda bermakna (p > 0,05). Kadar Pb darah dan krom di sentra SB dan FA berbeda bermakna dengan di sentra SA (p < 0,05). Tidak terdapat korelasi yang signifikan secara statistik antara kadar Pb darah, Cr darah, dan Pb limbah dengan kadar Hb dan PCV. Gejala iritasi kulit, pusing, dan nyeri sendi dirasakan oleh 36,8-48,7% responden. Kadar timbal dan krom darah pembatik di sentra SB dan FA lebih tinggi dari pada di sentra SA. Tidak terdapat hubungan bermakna antara toksisitas Pb dan Cr dengan kadar Hb dan PCV. Gejala toksisitas di antara pekerja batik diperkirakan dapat terjadi karena toksisitas logam berat kronis akibat pemakaian cat batik. KATA KUNCI batik; hemoglobin; kromium; sel darah; timbal ABSTRACT The dye used to color batik has changed from natural to synthetic materials in several places in Indonesia. Synthetic paints often contain heavy metals including lead (Pb) and chromium (Cr). Chronic use of heavy metals can affect health. This study was conducted to examine the relationship between blood and waste water levels of Pb and Cr with hemoglobin (Hb) and packed cell volume (PCV), and to determine how many workers felt the toxicity symptoms in three batik centers in Lendah, Kulon Progo. A total of 76 batik center employees, consisted of 37, 20, and 19 people from the SB, FA, and SA centers were recruited. Determination of Hb, PCV, Pb and Cr levels were conducted with the inductively coupled plasma (ICP) method. Waste water from the three batik centers is taken and examined. The data were analyzed by Oneway ANOVA or Kruskal-Wallis. Correlation analyses were performed with Pearson or Spearman correlation test. Results were considered significant if p < 0.05. Disease symptoms experienced by workers that were estimated to be associated with heavy metal toxicity were calculated in frequency. Anthropometrics, blood pressure, Hb levels, PCV and heavy metals levels were not significantly different between centers (p>0.05). Blood Pb and Cr from the SB and FA were significantly different from those at the SA center (p<0.05). There were no statistically significant correlations between blood Pb levels, blood Cr, and Pb waste with hemoglobin and PCV levels. Symptoms of skin irritation, dizziness, and joint pain were felt by 37-49% of respondents. Blood levels of Pb and Cr in the SB and FA centers are higher than in the SA centers. There was no significant correlation between Pb and Cr toxicity with hemoglobin and PCV levels. Symptoms of toxicity among batik workers are thought to occur due to chronic heavy metal toxicity due to the use of synthetic paint.KEYWORDS batik; haemoglobin; chromium; blood cell; plumbum

Hypothyroidism and stunting around the Merapi Volcano Pramudji Hastuti; Ahmad Hamim Sadewa; Prasetyastuti -; Sunarti -; Arta Farmawati; Ngadikun -; Dianandha Septiana Rubi; Abrory Agus Cahya Pramana
Journal of Community Empowerment for Health Vol 4, No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.61025

Cangkringan is a mountainous area with the possibility of low iodine sources in the soil. Additionally, Cangkringan area is the area nearest to where eruptions of Merapi mountain have occurred, which further could reduce iodine levels in the soil. This study examined the incidence of hypothyroidism due to iodine deficiency and potential links to stunting events on the slopes of Merapi mountain. By using ELISA methods, a total of 97 mothers were tested to detect the triiodothyronine (T3) and thyroid-stimulating hormone (TSH) levels to determine the frequency of hypothyroidism, while 97 children were assessed to check the stunting rates by measuring height compared to age. We found hypothyroidism was 3 (3.1%) out of 97 mothers examined and 30 (30.9%) out of 97 children examined were stunted (18.5% short stature and 12.4% very short stature). There was a significant difference between the mothers’ TSH levels among very short stature and normal stature. We concluded that hypothyroidism in mothers is correlated with stunting children in Cangkringan, Sleman sub-district. Further research is needed to determine the frequency of hypothyroidism and stunting in other slopes of Merapi mountain, where the low-iodine soil composition is potentially related to hypothyroidism and stunting incidence. Thus, further treatment is needed by local health staff and governments to address the negative effects of hypothyroidism and stunting.

Pengenalan Nutrigenomik Pada Guru SMA Biologi dan Kimia di Kota Yogyakarta Melalui Program Kemitraan Masyarakat (PKM) Arta Farmawati; Sunarti Sunarti; Dianandha Septiana Rubi; Ahmad Hamim Sadewa; Prasetyastuti Prasetyastuti; Ngadikun Ngadikun
SWARNA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2024): SWARNA: Jurnal Pengabdian Kepada Masyarakat, Februari 2024
Publisher : LPPM Sekolah Tinggi Ilmu Ekonomi 45 Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/swarna.v3i2.1177

Perkembangan ilmu pengetahuan dan teknologi berdampak pada pengembangan ilmu nutrisi berbasis molekuler diantaranya adalah nutrigenomik. Nutrigenomik merupakan ilmu yang mempelajari bagaimana nutrisi mempengaruhi ekspresi gen. Gen membawa deoxyribose nucleic acid (DNA) yang berisi informasi genetik yang menentukan sintesis protein, dimana protein tersebut berperan dalam berbagai proses biologis, sehingga sangat mempengaruhi kesehatan. Proses biologis merupakan proses reaksi kimia dalam tubuh yang sudah dipelajari sejak sekolah menengah pertama (SMP) oleh karena itu, pengenalan ilmu nutrigenomik pada guru SMA diharapkan dapat meningkatkan pengetahuan terhadap ilmu nutrigenomik pada guru, sehingga dapat meningkatkan kualitas siswa maupun guru sendiri. Upaya ini kami wujudkan dalam bentuk pengabdian masyarakat yang bekerja sama dengan Musyawarah Guru Mata Pelajaran (MGMP) Biologi dan Kimia Kota Yogyakarta. Kegiatan pelatihan yang diikuti 18 guru dilaksanakan dengan beberapa metode pembelajaran seperti ceramah, praktik di laboratorium, tutorial, dan training of trainer (ToT). Berdasarkan hasil evaluasi, program kemitraan masyarakat (PKM) dapat meningkatkan pengetahuan guru-guru mengenai nutrigenomik secara signifikan (43,33 ± 10,01 vs 66,44 ± 13,66) (P<0,05). Hasil ToT menunjukkan tingkat kepercayaan diri guru-guru dalam menyampaikan informasi terkait dengan nutrigenomik menjadi tinggi. Dengan demikian, program ini dapat mengenalkan sekaligus meningkatkan pengetahuan dan rasa percaya diri guru sebagai kader nutrigenomik untuk menyebarluaskan ilmu nutrigenomik kepada siswa maupun masyarakat.

Pengenalan Nutrigenomik Pada Guru SMA Biologi dan Kimia di Kota Yogyakarta Melalui Program Kemitraan Masyarakat (PKM) Farmawati, Arta; Sunarti, Sunarti; Rubi, Dianandha Septiana; Sadewa, Ahmad Hamim; Prasetyastuti, Prasetyastuti; Ngadikun, Ngadikun
SWARNA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2024): SWARNA: Jurnal Pengabdian Kepada Masyarakat, Februari 2024
Publisher : LPPM Sekolah Tinggi Ilmu Ekonomi 45 Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/swarna.v3i2.1177

Perkembangan ilmu pengetahuan dan teknologi berdampak pada pengembangan ilmu nutrisi berbasis molekuler diantaranya adalah nutrigenomik. Nutrigenomik merupakan ilmu yang mempelajari bagaimana nutrisi mempengaruhi ekspresi gen. Gen membawa deoxyribose nucleic acid (DNA) yang berisi informasi genetik yang menentukan sintesis protein, dimana protein tersebut berperan dalam berbagai proses biologis, sehingga sangat mempengaruhi kesehatan. Proses biologis merupakan proses reaksi kimia dalam tubuh yang sudah dipelajari sejak sekolah menengah pertama (SMP) oleh karena itu, pengenalan ilmu nutrigenomik pada guru SMA diharapkan dapat meningkatkan pengetahuan terhadap ilmu nutrigenomik pada guru, sehingga dapat meningkatkan kualitas siswa maupun guru sendiri. Upaya ini kami wujudkan dalam bentuk pengabdian masyarakat yang bekerja sama dengan Musyawarah Guru Mata Pelajaran (MGMP) Biologi dan Kimia Kota Yogyakarta. Kegiatan pelatihan yang diikuti 18 guru dilaksanakan dengan beberapa metode pembelajaran seperti ceramah, praktik di laboratorium, tutorial, dan training of trainer (ToT). Berdasarkan hasil evaluasi, program kemitraan masyarakat (PKM) dapat meningkatkan pengetahuan guru-guru mengenai nutrigenomik secara signifikan (43,33 ± 10,01 vs 66,44 ± 13,66) (P<0,05). Hasil ToT menunjukkan tingkat kepercayaan diri guru-guru dalam menyampaikan informasi terkait dengan nutrigenomik menjadi tinggi. Dengan demikian, program ini dapat mengenalkan sekaligus meningkatkan pengetahuan dan rasa percaya diri guru sebagai kader nutrigenomik untuk menyebarluaskan ilmu nutrigenomik kepada siswa maupun masyarakat.

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