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O Suprijana
Jurusan Kimia FMIPA Universitas Padjadjaran, Jatinangor, Sumedang 45363
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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ANTI JAMUR HASIL FERMENTASI Streptomyces Isp. 192 PADA MEDIA PATI DAN GLUKOSA S Djajasupena; O Suprijana; S.A Desak Gede; A T.Karossi
Jurnal Kimia Terapan Indonesia Vol 11, No 2 (2009)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3158.863 KB) | DOI: 10.14203/jkti.v11i2.162

Abstract

Streptomyces ISP 192 was family of Actinomycetes which was high value microorganism that industrially important because it was capablefor producing ofbioactive compound that very useful in the field of medicine that is antibiotic. Recently antibiotic is still eligible medicine to overcome infection disease, but many more organism that cause of infection have been resistant against various antibiotics so need to look for a new antibiotic. This research cover cultivation of Streptomyces ISP 192 in potato dextrose agar (PDA) medium then fermentation by 250 mL shakeflash with 50 ml working volume. Microorganism was inoculated in fermentation medium MF1 and MF2 that contain (%)w/v variation of starch and glucose to bea source of carbon as soon as (%)v/v variation of inoculum. Fermentation process was conducted in 30°C temperature with 150 rpm shaking for seven days. Regularly for each 24 hour, sample was taken for analyzing ofpH, glucose and protein concentration, dry weight of cell and testing of anti fungal activity against Candida albicans, Microsporum gypseum, Tricophyton sp. dan Aspergillus niger. The result of research showed Streptomyces ISP 192 has high anti fungal activities against Candida albicans, Microsporum gypseum, Tricophyton sp., in usage of fementation media MF2 with 10 % glucose to be a carbonsource and 10% inoculum but not have activited against Aspergillus niger. Biosynthesis of antibiotic anti fungal start from 4th to 6th day that indication indecreasing of glucose and protein concentration as well aspH.Keywords: Streptomyses ISP 192, fermentation, testing ofantifungal activities.
PENGARUH KONSENTRASI PENGINDUKSI METANOL SERTA"SUMBER KARBON SORBITOL DAN MONITOL TERHADAP PRODUKSI a-AMILASE Saccharomycopsis fibuligera R64 DALAM Pichia pastoris Shabarni Gaffar; Dani Permana; Diana P Rahmawati; Triana N Meirina; Abu Bakar M.I. Syihab; Safrl Ismayana; Toto Subroto; O Suprijana
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5297.927 KB) | DOI: 10.14203/jkti.v13i2.152

Abstract

Pichia pastoris has been widely used as host for heterologous protein expression for commercial purposes. The advantages of P. pastoris as protein expression host is its ability to grow with high cell density and the presence of a gene promoter that canbe induced tightly, named AOXI encoding alcohol oxidase. Improvement of recombinant protein production under control of AOXI promoter in P. pastoris expression system is still a major concern. Optimization of methanol concentration as inducerand addition of carbon sources, is one of the strategies to improve the expression level. This research aims to study the effect of methanol as inducer as well as sorbitol and mannitol as additional carbon source to the expression level of recombinantSaccharomycopsis fibuligera a-amylase (Sfamy) by P. pastoris (Mut). Sorbitol and manuot known as non-repressive carbon source, to increase the growth ofP. past oris, but not inhibit the AOX1 promoter and foreign proteins expression. Sfamy was expressed in P. pastoris GS115 (His-, Mut) with addition ofcarbon source, sorbitol and mannitol saparately to the expression medium. The result showed that, the optimum concentration" of methanol inducer for Sfamy production is 0.75%. The addition of sorbitol or mannitol increased Sfamy production. Concentrations of sorbitol and mannitol 2% with0.75% methanol inducer increase the secretion level of recombinant Sfamy 2.13 times and 1.94 times respectively, compared with no additional carbon source. This result indicated that addition of carbon source can improved recombinant protein production by P. pastoris, and the used of sorbitol as additionalcarbon source is more effective compared to mannitol.Key words: a-amylase. Pichid pastoris, methanol, sorbitol,mannitol.
Co-Authors A T.Karossi Abu Bakar M.I. Syihab Dani Permana Diana P Rahmawati S Djajasupena S.A Desak Gede Safrl Ismayana Shabarni Gaffar Toto Subroto Triana N Meirina