Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Kimia Terapan Indonesia JAKBS
Dani Permana
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Materials Science & Nanotechnology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
PENGARUH KONSENTRASI PENGINDUKSI METANOL SERTA"SUMBER KARBON SORBITOL DAN MONITOL TERHADAP PRODUKSI a-AMILASE Saccharomycopsis fibuligera R64 DALAM Pichia pastoris Shabarni Gaffar; Dani Permana; Diana P Rahmawati; Triana N Meirina; Abu Bakar M.I. Syihab; Safrl Ismayana; Toto Subroto; O Suprijana
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5297.927 KB) | DOI: 10.14203/jkti.v13i2.152

Abstract

Pichia pastoris has been widely used as host for heterologous protein expression for commercial purposes. The advantages of P. pastoris as protein expression host is its ability to grow with high cell density and the presence of a gene promoter that canbe induced tightly, named AOXI encoding alcohol oxidase. Improvement of recombinant protein production under control of AOXI promoter in P. pastoris expression system is still a major concern. Optimization of methanol concentration as inducerand addition of carbon sources, is one of the strategies to improve the expression level. This research aims to study the effect of methanol as inducer as well as sorbitol and mannitol as additional carbon source to the expression level of recombinantSaccharomycopsis fibuligera a-amylase (Sfamy) by P. pastoris (Mut). Sorbitol and manuot known as non-repressive carbon source, to increase the growth ofP. past oris, but not inhibit the AOX1 promoter and foreign proteins expression. Sfamy was expressed in P. pastoris GS115 (His-, Mut) with addition ofcarbon source, sorbitol and mannitol saparately to the expression medium. The result showed that, the optimum concentration" of methanol inducer for Sfamy production is 0.75%. The addition of sorbitol or mannitol increased Sfamy production. Concentrations of sorbitol and mannitol 2% with0.75% methanol inducer increase the secretion level of recombinant Sfamy 2.13 times and 1.94 times respectively, compared with no additional carbon source. This result indicated that addition of carbon source can improved recombinant protein production by P. pastoris, and the used of sorbitol as additionalcarbon source is more effective compared to mannitol.Key words: a-amylase. Pichid pastoris, methanol, sorbitol,mannitol.
PEMANFAATAN SISTEM MICROBIAL FUEL CELL DALAM MENGHASILKAN LISTRIK PADA PENGOLAHAN AIR LIMBAH INDUSTRI PANGAN Herlian E. Putra; Dani Permana; Agusta S. Putra; Djaenudin Djaenudin; Hari R. Haryadi
Jurnal Kimia Terapan Indonesia Vol 14, No 2 (2012)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.503 KB) | DOI: 10.14203/jkti.v14i2.341

Abstract

Microbial Fuel Cell (MFC) dapat langsung menghasilkan listrik dari oksidasi bahan organik yangterkandung dalam suatu larutan. Dibutuhkan suatu optimalisasi sistem MFC yang mencakup beberapa faktoruntuk meningkatkan hasil listrik seperti jenis media penukar kation, elektroda, luas permukaan elektroda, dandurasi reaksi. Penelitian dilakukan untuk melihat kinerja sistem MFC pada pengolahan air limbah industripangan, dalam  hal ini tahu  dan cucian beras (catering),  dengan  menggunakan tembaga sebagai elektroda, membran Poli Eter Eter Keton Tersulfonasi (SPEEK) sebagai media penukar kation, dan menggunakan mediasubstrat limbah. MFC diamati selama 80 jam. Pengukuran tegangan yang dihasilkan dilakukan setiap 4 jam.Tegangan maksimum yang dihasilkan pada media limbah tahu mencapai 80 mV (pada jam ke-28). Selama 52jam berikutnya, tegangan sedikit menurun. Sedangkan pada air limbah cucian beras, tegangan maksimum terjadipada jam ke-60 yaitu 234 mV. Selama proses 80 jam, melalui sistem MFC pada media limbah tahu, CODmampu turun 49,33% dari 6750 mg/L menjadi 3420 mg/L. Sedangkan pada limbah cucian beras, COD turundari 18840 mg/L menjadi 10560 mg/L atau sebesar 43,95 % selama 80 jam.Kata Kunci: Microbial Fuel Cell, listrik, limbah pangan.
Pengaruh Kemajuan Digital Terhadap Perubahan Media Investasi Gen Z Pada Mahasiswa UMBY Dani Permana; Maria Chandrayani Bai Nai; M.Rizky Ardi Saputra
Jurnal Akuntansi Keuangan dan Bisnis Vol. 1 No. 4 (2024): Januari - Maret
Publisher : CV. ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research uses a positivism paradigm and quantitative descriptive research methods using questionnaires. The target population of this research is regular morning students at Mercu Buana University, Yogyakarta. Samples will be taken using Slovin Formula This research aims to investigate how the development of digital technology and the internet has influenced the way Generation Z (students) use media for investment purposes. The main focus of the research is how to understand the shift in media preferences used by Generation Z in the context of investment, finance and how this is reflected in their behavior along with the development of digital technology, this research also includes an analysis of its impact on investment decisions, as well as the potential for changes in the investment paradigm of generation z in the context of higher education, the sample collection method uses a probability sampling technique using a simple random sample. random sampling) research variables consist of independent variables (digital progress) and dependent variables (changes in investment media)
Co-Authors Abu Bakar M.I. Syihab Agusta S. Putra Diana P Rahmawati Djaenudin Djaenudin Hari R. Haryadi Herlian E. Putra M.Rizky Ardi Saputra Maria Chandrayani Bai Nai O Suprijana Safrl Ismayana Shabarni Gaffar Toto Subroto Triana N Meirina