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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Indonesian Journal of Biotechnology
Siti Isrina Oktavia Salasia
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Veterinary Other

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Journal : Jurnal Veteriner

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Distribusi Gen Enterotoksin Staphylococcus aureus dari Susu Segar dan Pangan Asal Hewan Siti Isrina Oktavia Salasia; Khusnan -; Sugiyono -
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Staphylococcus aureus is a potential pathogen causing disease in human and animals due to severalvirulence factors. Staphylococcal enterotoxins which is responsible for foodborne disease is considered tobe one of the important virulence factor for the bacteria. The research was conducted to identify variousenterotoxin genes of S. aureus. Twenty three S. aureus isolates from milk cows (12 isolates) and foodanimal products (11 isolates) were used to study various enterotoxins genes (sea, seb, sec, sed, see, seg, seh,sei, and sej). The enterotoxins genes of S. aureus were investigated by using polymerase chain reaction(PCR) with specific primers. There were 3 isolates (13.04%) negative for staphylococcal enterotoxin genes.Twenty isolates (86.96%) harboured for one or more staphylococcal enterotoxin genes such as: sec (6 isolates/26.09%), see, seh for 1 isolate (4.35%), combination of 2 genes se (b,i), se (c,g), se (g,i) for 1 isolate of each(4.35%), se(c,e) for 2 isolates (8.70%), se(b,c) for 4 isolates (17.39%). Staphylococcal enterotoxin could bedetected in 3 combination genes of se(b,c,i), se(c,e,i), se(c,g,i) for 1 isolate of each (4.35%).
ISOLATION AND PHENOTIPICAL CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM SLAUGHTERED HOUSE WASTE AND CARCASS OF CHICKEN Khusnan -; Siti Isrina Oktavia Salasia; Sugiyono -
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Staphylococcus aureus is known as a potential agent that cause food poisoning. Chicken meats could be as vehicles of food poisoning cases while they contaminated by agents during slaughtered and meat processing as well as infected chickens by S. aureus itself. The aims of the research were to isolated and characterized of S. aureus from liquid wastes of chicken slaughtered house and washing water of chicken carcass. The characterization of S aureus based on mannitol salt agar (MSA), catalase and coagulation assay, production of hemolysins, hydrophobicity test, and the reaction of hemaglutination. Thirty seven samples used in this study were collected from 16 liquid wastes slaughtered chicken house and 21 samples from washing water of chicken carcasses.The research resulted 18 S. aureus isolated from liquid wastes slaughtered house (4 isolates) and from wahing water of chicken carcasses (14 isolates). All 18 isolates fermented mannitol, catalase and coagulase positive, contain of hydrophilic surfaces. Among 18 S. aureus isolates could agglutinate of rabbit erythrocytes for 55%, produced ?-hemolysis (12%) and ?-hemolysis (88%).
Co-Authors Afif Nurul Hidayati Christin Marganingsih Santosa Dwi Ariyanti Fikri Ardhani Khusnan k Sigit Eko Prabowo Sugiyono - Syarifudin Tato