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All Journal Jurnal Sain Veteriner Buletin Peternakan Indonesian Journal of Biotechnology
Siti Isrina Oktavia Salasia
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Veterinary Other

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Characterization of Haemolysin of Staphylococcus aureus Isolated from Food of Animal Origin Dwi Ariyanti; Siti Isrina Oktavia Salasia; Syarifudin Tato
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.885 KB) | DOI: 10.22146/ijbiotech.7834

Abstract

Staphylococcus aureus is an important pathogen bacteria causing food poisoning and various infection in animals and humans. Haemolysin is one of the virulence factors of Staphylococcus aureus. The aims of the research were to characterize haemolysins of Staphylococcus aureus isolated from various food of animal origin, phenotypic- and genotypically. In the present study, eleven Staphylococcus aureus isolated from various food of animal origins from traditional markets and supermarkets in Yogyakarta, Sidoarjo, Jakarta, and Bandung were characterized for haemolysin, pheno- and genotypically. Characterization of haemolysin phenotypically based on haemolysis pattern of Staphylococcus aureus on sheep blood agar plate. Genes encoding hemolysin were amplified with specific primers by using polymerase chain reaction (PCR) technique. The results of the studies showed that Staphylococcus aureus on sheep blood agar plates revealed an alpha haemolysis pattern (18,18%), beta haemolysis (27,27%) and gamma haemolysis (54,55%). Based on amplification of the gene encoding haemolysin of Staphylococcus aureus with specific primers showed hla genes (81,81%), and hla combined with hlb genes (18,18%). The amplification of gene hla and hlb had a single amplicon with a size of approximately 534 bp and 833 bp, respectively. The haemolysin characteristics of Staphylococcus aureus from various food of animal origin could be used as important information to control staphylococcal food poisoning.Keywords : Staphylococcus aureus, haemolysin, PCR, food of animal origins
Respon Neutrofil, Adesi Pada Sel Epitel, Aglutinasi Eritrosit Terhadap Staphylococcus aureus : Kajian Hidrofobisitas In Vitro = Response of neutrophils, epithelial cells adhesion, erythrocytes agglutination of Staphyloco Khusnan .; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2690.953 KB) | DOI: 10.22146/jsv.345

Abstract

Staphylococcus aureus merupakan salah satu bakteri potensial sebagai penyebab utama mastitis pada sapi perah. Mastitis dapat menyebabkan kerugian ekonomi peternak akibat turunnya produksi susu. Infeksi bakteri dapat terjadi melalui kemampuan bakteri memasuki hospes, berkembang biak, merusak jaringan inang dan mampu bertahan dalam tubuh hospes. Penelitian ini bertujuan untuk mengetahui hubungan sifat hidrofobisitas S. aureus dan kemampuannya terhadap aglutinasi eritrosit, pelekatan dengan sel epitel dan kemampuan bertahan terhadap fagositosis sel polimorfonuklear. Dari 10 isolat S. aureus yang digunakan dalam penelitian ini, terdapat 8 isolat bersifat hidrofob dan 2 isolat bersifat hidrofil. Diantara isolat yang bersifat hidrofob terdapat 2 isolat mempunyai kemampuan mengaglutinasi eritrosit sapi perah, kambing, domba. Staphylococcus aureus yang bersifat hidrofob dan hemaglutinasi positif, lebih banyak melekat pada sel-sel epitel bukalis dan lebih banyak difagosit oleh sel-sel PMN dibanding isolat yang bersifat hidrofob tetapi hemaglutinasi negatif maupun isolat yang bersifat hidrofil. Isolat yang bersifat hidrofil tidak mampu mengaglutinasi eritrosit dan lebih sedikit melekat pada sel-sel epitel dan lebih sedikit difagosit oleh sel-sel PMN.
Efek Ekstrak Daun Bangun-Bangun (Coleus ambonicus, L.) pada Aktivitas Limfosit Tikus Putih Christin Marganingsih Santosa; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1392.143 KB) | DOI: 10.22146/jsv.483

Abstract

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Efek Ekstrak Air Daun Bangun-Bangun (Coleus ambonicus, L) pada Aktivitas Limfosit Tikus Putih Christin Marganingsih Santosa; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1392.143 KB) | DOI: 10.22146/jsv.484

Abstract

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Deteksi Protein Spesifik untuk Membedakan Daging Babi dengan Berbagai Macam Daging Spesies Lain Fikri Ardhani; Sigit Eko Prabowo; Afifah Nurul Hidayati; Siti Isrina Oktavia Salasia
Buletin Peternakan Vol 31, No 1 (2007): Buletin Peternakan Vol. 31 (1) Februari 2007
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v31i1.1216

Abstract

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Co-Authors Afif Nurul Hidayati Christin Marganingsih Santosa Dwi Ariyanti Fikri Ardhani Khusnan k Sigit Eko Prabowo Sugiyono - Syarifudin Tato